DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment cass and API5

DIOPT Version :9

Sequence 1:	NP_477454.1	Gene:	cass / 35053	FlyBaseID:	FBgn0286781	Length:	536	Species:	Drosophila melanogaster
Sequence 2:	NP_001136402.1	Gene:	API5 / 8539	HGNCID:	594	Length:	524	Species:	Homo sapiens

Alignment Length:	539	Identity:	249/539 - (46%)
Similarity:	337/539 - (62%)	Gaps:	28/539 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDNIERLYKCYEILSEAGDKISEHVDEYKEILKAVKGTSKEKRLASQFIGNFFKHFPDLADTAID 65
            |..:|.||:.|.||::|.:::.:|.|.|:.||..|||.:||||||:|||..||||||:|||:||:
Human     1 MPTVEELYRNYGILADATEQVGQHKDAYQVILDGVKGGTKEKRLAAQFIPKFFKHFPELADSAIN 65

  Fly    66 AQFDLCEDDDTQIRRQAIKDLPKLCQGNADATIRVGDTLAQLLILDDPTELQQVNNSLLAIIKLD 130
            ||.|||||:|..|||||||:||:...|  :...||.|.|.|||..||..|...|||:||:|.|:|
Human    66 AQLDLCEDEDVSIRRQAIKELPQFATG--ENLPRVADILTQLLQTDDSAEFNLVNNALLSIFKMD 128

  Fly   131 TKSSIAGLFQQISTGDETTRERCLKFIATKLLTMGPTVITKEIEDYIVEEIKKALQDVTADEFHL 195
            .|.::.|||.||..|::..|||.:||::|||.|:...|:|||:|:.|:.|.||.|:|||.:||.|
Human   129 AKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPDEVLTKEVEELILTESKKVLEDVTGEEFVL 193

  Fly   196 CMTILGATKLGSTITGHAELVKLATEQAELNNTDADIIAVDDEVVERFIQCASAAAPYFSKTIKS 260
            .|.||...|...|::|..:||:|..|||:|..|   ....|.:.|:|.:||...|.|.|||.:.|
Human   194 FMKILSGLKSLQTVSGRQQLVELVAEQADLEQT---FNPSDPDCVDRLLQCTRQAVPLFSKNVHS 255

  Fly   261 TAFVAHVCDKLLPIKTWNMIATAVSQDQIQLRLLKVFAEMITNTDKLDNASERINAVYNVLLEYM 325
            |.||.:.|:::||  ....:.|.|....|||.:||:.|||.:....::.....:..:::.|||||
Human   256 TRFVTYFCEQVLP--NLGTLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLETNLRKLFDKLLEYM 318

  Fly   326 PLP---KLSDEDLGDTPPSFQFSHAECLLYALHTLGKNHPNSLSFVEDAEKLKDFRARLQYLARG 387
            |||   ..:.|:.|:..|..|||:.|||||:.|.||:..|:.|:...:|||||||:.||||.|||
Human   319 PLPPEEAENGENAGNEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARG 383

  Fly   388 TQGYIKKLEESLKGKTGEELKTEENQLKQTALKTTSNINILIRDLFHSPPIFKHDIVLSWIVPKN 452
            .|.||::|..:|:|||||.||||||::|..|||.|:|||:||:||||.||.:|..:.|||
Human   384 LQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSW----- 443

  Fly   453 NKLGKRHAPITFGEKAAANGKDKDQEPEKKSR-PSND-QKFYSPPSGKYSNKVNQSYGNNNRTRQ 515
                |....:..|:|.|:........|:|.|. |..| ::.|:|||||||:.:    ||.| ..|
Human   444 ----KPVQKVEIGQKRASEDTTSGSPPKKSSAGPKRDARQIYNPPSGKYSSNL----GNFN-YEQ 499

  Fly   516 RGG--GGGGGSGGGYRNRR 532
            ||.  |..||.|.|.|..|
Human   500 RGAFRGSRGGRGWGTRGNR 518

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
cass	NP_477454.1	API5	4..505	CDD:283550	235/505 (47%)
API5	NP_001136402.1	ARM-like and Heat-like helical repeats	2..360		167/364 (46%)
		API5	4..496	CDD:399130	237/511 (46%)
		HEAT repeat	63..91	CDD:293787	19/27 (70%)
		HEAT repeat	97..139	CDD:293787	22/41 (54%)
		Leucine-zipper	370..391		14/20 (70%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	452..524		29/72 (40%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:22334682	454..475		6/20 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165155360
Domainoid	1	1.000	440	1.000	Domainoid score	I557
eggNOG	1	0.900	-	-		E1_KOG2213
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4809
Inparanoid	1	1.050	443	1.000	Inparanoid score	I1658
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54362
OrthoDB	1	1.010	-	-		D1385954at2759
OrthoFinder	1	1.000	-	-		FOG0006081
OrthoInspector	1	1.000	-	-		oto88711
orthoMCL	1	0.900	-	-		OOG6_105172
Panther	1	1.100	-	-	LDO	PTHR12758
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4470
SonicParanoid	1	1.000	-	-		X3853
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.800