DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Dif and Nfkb2

DIOPT Version :9

Sequence 1:	NP_001162998.1	Gene:	Dif / 35045	FlyBaseID:	FBgn0011274	Length:	987	Species:	Drosophila melanogaster
Sequence 2:	NP_001008350.1	Gene:	Nfkb2 / 309452	RGDID:	1307189	Length:	898	Species:	Rattus norvegicus

Alignment Length:	922	Identity:	230/922 - (24%)
Similarity:	345/922 - (37%)	Gaps:	266/922 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    70 PEPSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETG-KTFPTIEVCNYDGPVIIV 133
            |.|....||:|.|||:|.....||||.|||.:.|.:||  :|||.| ||:||:::|||:||..|.
  Rat    30 PAPETADGPYLVIVEQPKQRGFRFRYGCEGPSHGGLPG--ASSEKGRKTYPTVKICNYEGPAKIE 92

  Fly   134 VSCVTSDEPFRQHPHWLVSKE--EADACKSGIYQKKLPPEERRL--------VLQKVGIQCAKKL 188
            |..||..:|.|.|.|.||.|:  |...|...:..|.:..:...|        .:.::.||..::.
  Rat    93 VDLVTHSDPPRAHAHSLVGKQCSELGVCAVSVGPKDMTAQFNNLGVLHVTKKNMMEIMIQKLQRQ 157

  Fly   189 EMR---DSLVERERRNIDPFNAKFDHKDQIDKINRYEL-RLCYQAFITV--GNSKVPLDPIVSSP 247
            .:|   ..|.|.|||.::. .||     ::.|:....: ||.:.||:..  |:..:||.|::|.|
  Rat   158 RLRSKPQGLTEAERRELEQ-EAK-----ELKKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQP 216

  Fly   248 IYGK----SSELTITRLCSCAATANGGDEIIMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQP 308
            |:..    :|.|.|:|:...|.:..||||:.:||:|:.||||||||||.|::|   |.|..:|.|
  Rat   217 IHDSKSPGASNLKISRMDKTAGSVRGGDEVYLLCDKVQKDDIEVRFYEDDENG---WQAFGDFSP 278

  Fly   309 TDVFKQMAIAFKTPRYRNTEITQSVNVELKLVRPSDGATSAPLPFEYYPNPGTVTFARLQRKLKR 373
            |||.||.||.|:||.|...:|.:.|.|.|:|.|...|..|....|.|||    :...:.:.:.||
  Rat   279 TDVHKQYAIVFRTPPYHKMKIERPVTVFLQLKRKRGGDVSDSKQFTYYP----LVEDKEEVQRKR 339

  Fly   374 RQELDVFQQILSLDS---------------------------------ETTATKYSCPP------ 399
            |:.|..|.|.....|                                 ::.|....|.|
  Rat   340 RKALPTFSQPFGGGSHMGGGSGGSAGGYGGAGGGGSLGFFSSFAYSPYQSGAAPMGCFPGGGGGA 404

  Fly   400 ------MDLE----EDNNTVSSD----DQQSSGTLNEF-EIAVKKMQMRQRGESHEMDGGTVTEY 449
                  .|.|    |:.....|:    :||:..||... |...:...:.||.....:|.|...
  Rat   405 QMAGSGRDAEAGEAEEQPRAPSEAPQSEQQALDTLQRAREYNARLFGLAQRSARALLDYGVTA-- 467

  Fly   450 TDNDNEPDMEIELEVPQLLPQLADDCTQTSTPMEEILQHPQLQSRPLSNVETITEWMKSSEFERT 514
                   |....|...:.|....|:  ...||:...:.|.|     ...:|.|.:.:..:::
  Rat   468 -------DARALLAGQRHLLMAQDE--NGDTPLHLAIIHGQ-----TGVIEQIAQVIYHAQY--- 515

  Fly   515 DSLTVENGDTQSLSNSTAQTAFTNVSSLTANTTITNQL--------------DEEAH-GLLANGS 564
              |.|.|     |:|...||.. :::.:|..|.:.:.|              |...| .|.|..:
  Rat   516 --LGVIN-----LTNHLHQTPL-HLAVITGQTRVVSFLLQVGADPTLLDRHGDSAVHLALRAGAA 572

  Fly   565 RRDTLEN---------PAVKELPENE-----------KEPE----MVSASAQAS----------M 595
            ..|.|:.         |.:..:|:.|           :.||    :|...|:..          :
  Rat   573 APDLLQAVLHSGAHALPQMLHMPDFEGLYPVHLAVHARSPECLDLLVDCGAEVEAAERQGGRTPL 637

  Fly   596 DLDENFDETATYTSL-----------QIAFNNPIDI----------------GLPKESKQAAPIY 633
            .|....:|....|.|           ..|.|.|:.:                |....::...|:.
  Rat   638 HLATEMEELGLVTHLVTKLHANVNARTFAGNTPLHLAAGLGSPTLTRLLLKAGADIHAENEEPLC 702

  Fly   634 P-PTNPFGQLDDDE----------------LVVLTNPPAPRIKVNAAQTPATPPASPPLPLPPRT 681
            | |:.|....|.|.                |.:..:.....:.:||||....||.:||.|..|..
  Rat   703 PLPSPPTSGSDSDSEGPETDTQRNFRGHTPLDLTRSTKVKTLLLNAAQNTTEPPLAPPSPAGPGL 767

  Fly   682 PSPVSDQRLPPLP-----PKPQRNSQDLSAVSDSISVSRSRNGSLS---SNRTTPSPI------- 731
              .:.|..|..|.     |:.|.:..:|:          .|.|..|   :.|.||||.
  Rat   768 --SLGDAALQNLEQLLDGPEAQGSWAELA----------ERLGLRSLVDTYRKTPSPSGSLLRSY 820

  Fly   732 ---------IIMRTPD----------QSPTKRQPTPSASPKKRQGFFSRFFSRRRSKTDDEESLT 777
                     ::....|          ::|..|...||...|:...:.|      :|...:.|.|.
  Rat   821 KLAGGDLVGLLEALSDMGLHEGVRLLKAPETRDKLPSTEVKEDSAYGS------QSVEQEAEKLC 879

  Fly   778 PGGSPNKQLPGG 789
            |...|    |||
  Rat   880 PPPEP----PGG 887

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dif	NP_001162998.1	RHD-n_Dorsal_Dif	78..252	CDD:143647	66/194 (34%)
Dif	NP_001162998.1	RHD_dimer	256..358	CDD:292797	48/101 (48%)
Nfkb2	NP_001008350.1	RHD-n_NFkB2	38..221	CDD:143650	66/190 (35%)
		IPT_NFkappaB	228..327	CDD:238582	48/101 (48%)
		ANK	482..619	CDD:238125	28/154 (18%)
		ANK repeat	486..523	CDD:293786	10/51 (20%)
		Ank_4	487..546	CDD:290365	15/74 (20%)
		ANK	520..687	CDD:238125	28/172 (16%)
		ANK repeat	525..556	CDD:293786	5/31 (16%)
		Ank_2	530..627	CDD:289560	16/97 (16%)
		ANK repeat	558..585	CDD:293786	6/26 (23%)
		ANK repeat	598..629	CDD:293786	5/30 (17%)
		Ank_2	604..697	CDD:289560	12/92 (13%)
		ANK repeat	632..664	CDD:293786	4/31 (13%)
		ANK repeat	666..697	CDD:293786	4/30 (13%)
		Death_NFkB2_p100	773..847	CDD:176776	14/83 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166351519
Domainoid	1	1.000	99	1.000	Domainoid score	I6882
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D391433at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24169
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.950