DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dif and Nfkb1

DIOPT Version :9

Sequence 1:	NP_001162998.1	Gene:	Dif / 35045	FlyBaseID:	FBgn0011274	Length:	987	Species:	Drosophila melanogaster
Sequence 2:	NP_032715.2	Gene:	Nfkb1 / 18033	MGIID:	97312	Length:	971	Species:	Mus musculus

Alignment Length:	1068	Identity:	247/1068 - (23%)
Similarity:	401/1068 - (37%)	Gaps:	267/1068 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    55 HIPLHLQNQNMNQNL--PE-PSARSGPHLRIVEEPTSNIIRFRYKCEGRTAGSIPGMNSSSETGK 116
            |:...|.:...|..|  || |.:..||:|:|:|:|.....||||.|||.:.|.:||. ||.:..|
Mouse    14 HLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGA-SSEKNKK 77

  Fly   117 TFPTIEVCNYDGPVIIVVSCVTSDEPFRQHPHWLVSKEEADACKSGIYQKKLPPEERRLVLQKVG 181
            ::|.:::|||.||..::|..||:.:....|.|.||.|.    |:.|:......|::..:....:|
Mouse    78 SYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKH----CEDGVCTVTAGPKDMVVGFANLG 138

  Fly   182 IQCAKKLEMRDSLVERER----RNIDPFNAKFDHKD----------------------------Q 214
            |....|.::.::|..|..    |..:|  ....|.|                            |
Mouse   139 ILHVTKKKVFETLEARMTEACIRGYNP--GLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQ 201

  Fly   215 IDKINRYELRLCYQAFI--TVGNSKVPLDPIVSSPIYG----KSSELTITRLCSCAATANGGDEI 273
            ..:::...:||.:.||:  :.|:....|:|:||..||.    .:|.|.|.|:...|....||:||
Mouse   202 TKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEI 266

  Fly   274 IMLCEKIAKDDIEVRFYETDKDGRETWFANAEFQPTDVFKQMAIAFKTPRYRNTEITQSVNVELK 338
            .:||:|:.||||::||||.:::| ..|....:|.||||.:|.||.||||:|::..||:..:|.::
Mouse   267 YLLCDKVQKDDIQIRFYEEEENG-GVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQ 330

  Fly   339 LVRPSDGATSAPLPFEYYPNPGTVTFARLQRKLKRRQELDVFQQILSLDSETTA----------- 392
            |.|.||..||.|.||.|||.  ......:||  ||::.:..|.......|...|
Mouse   331 LRRKSDLETSEPKPFLYYPE--IKDKEEVQR--KRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGG 391

  Fly   393 -------------TKYSCPPM-DLEEDNNTVSSDDQQSSGTLN-EFEIAVKKMQMRQRGESHEMD 442
                         :.|..||. .:........|:...:.||:| :|:...|........||..:
Mouse   392 GGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHGTINTKFKNGPKDCAKSDDEESLTL- 455

  Fly   443 GGTVTEYTDNDNEPDMEIELEVPQLLPQLADDCTQTSTPMEEILQHPQLQSRPLSNVETITEWMK 507
                         |:.|.|.|.|. ||..   ||:|               .|::...|:.:  |
Mouse   456 -------------PEKETEGEGPS-LPMA---CTKT---------------EPIALASTMED--K 486

  Fly   508 SSEFERTDSLTVE------------------NGDTQSLSNSTAQTAFTNVSS-----------LT 543
            ..:....|:|.:|                  .||.:.|  ...|...|.|..           :.
Mouse   487 EQDMGFQDNLFLEKALQLARRHANALFDYAVTGDVKML--LAVQRHLTAVQDENGDSVLHLAIIH 549

  Fly   544 ANTTITNQLDEEAHGLLAN---GSRRDTLENPAVKELPENEKEPEMVSASAQASMDLD--ENFDE 603
            .:..:...|.|...||:::   ..|.|..:.|.  .|....|:.::|....:...||.  :.:..
Mouse   550 LHAQLVRDLLEVTSGLISDDIINMRNDLYQTPL--HLAVITKQEDVVEDLLRVGADLSLLDRWGN 612

  Fly   604 TATYTSLQIAFNNPIDIGLPKESKQAAPIYPPTNPFGQLDDDELVVLTNPPAPRIKVNAAQTPAT 668
            :..:.:.:...:..:.|.|  :|::|||:....|..| |:...:.|::|
Mouse   613 SVLHLAAKEGHDRILSILL--KSRKAAPLIDHPNGEG-LNAIHIAVMSN---------------- 658

  Fly   669 PPASPPLPLPPRTPSPVSDQRLPPLPPKPQRNSQDLSAVSDSISVS-------RSRNGSLSSNRT 726
              :.|.|.|.....:.|:.|.     .|..|.:..|:...|:||::       .:...|.:.:.|
Mouse   659 --SLPCLLLLVAAGAEVNAQE-----QKSGRTALHLAVEYDNISLAGCLLLEGDAHVDSTTYDGT 716

  Fly   727 TPSPIIIMRTPDQSPTKRQPTPSASPKKRQGF------FSRFFSRRRS--KTDDEESLTPGGSP- 782
            ||..|         ...|..|..|:..|..|.      |...:....|  |..::|.:.||.:|
Mouse   717 TPLHI---------AAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWEKAGEDEGVVPGTTPL 772

  Fly   783 ----NKQ-------------------LPGGSKATTPTGSREPSVAHFSLGDPNRSSTRSMQPLGK 824
                |.|                   ||.|........:|........:.||:::.....|.||.
Mouse   773 DMAANWQVFDILNGKPYEPVFTSDDILPQGDMKQLTEDTRLQLCKLLEIPDPDKNWATLAQKLGL 837

  Fly   825 SEGRNGKPVGRSSSSVSGKRPAHLDADVIHIPLKGGDSENSL--LRPESYSNASTL---SYGRPL 884
            ....|...:.          ||.....:.:..:.||..:..:  |:...|:.|..:   ::..|.
Mouse   838 GILNNAFRLS----------PAPSKTLMDNYEVSGGTIKELMEALQQMGYTEAIEVIQAAFRTPA 892

  Fly   885 DRKT--LSTLQLADIPISDGNMELIAIADRQSIKNLCEGAYGVVLDPSVD-----LSEAEHFALY 942
            ...:  ::|.|:..:|:|..       :.||.|..|.:.  ..|.|..|:     ||..|..
Mouse   893 TTASSPVTTAQVHCLPLSSS-------STRQHIDELRDS--DSVCDSGVETSFRKLSFTESL--- 945

  Fly   943 TSKSP-----EPPFGGECGEGGAATGGV 965
            |..||     :.|.|  .|:.|...|.:
Mouse   946 TGDSPLLSLNKMPHG--YGQEGPIEGKI 971

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dif	NP_001162998.1	RHD-n_Dorsal_Dif	78..252	CDD:143647	56/211 (27%)
Dif	NP_001162998.1	RHD_dimer	256..358	CDD:292797	47/101 (47%)
Nfkb1	NP_032715.2	RHD-n_NFkB1	40..241	CDD:143651	56/207 (27%)
		IPT_NFkappaB	248..349	CDD:238582	47/101 (47%)
		Nuclear localization signal. /evidence=ECO:0000255	358..363		4/6 (67%)
		GRR	370..392		2/21 (10%)
		Interaction with CFLAR. /evidence=ECO:0000250	433..971		120/635 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	439..470		10/45 (22%)
		ANK repeat	538..575	CDD:293786	4/36 (11%)
		Ank_4	539..598	CDD:290365	10/60 (17%)
		ANK	572..701	CDD:238125	30/156 (19%)
		ANK repeat	577..608	CDD:293786	6/32 (19%)
		Ank_2	582..676	CDD:289560	20/116 (17%)
		Essential for interaction with HIF1AN. /evidence=ECO:0000250	646..680		9/57 (16%)
		ANK repeat	646..677	CDD:293786	8/49 (16%)
		Ank_5	666..722	CDD:290568	13/69 (19%)
		ANK repeat	680..712	CDD:293786	6/31 (19%)
		Death_NFkB1_p105	812..886	CDD:260063	14/83 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167847945
Domainoid	1	1.000	115	1.000	Domainoid score	I6003
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24169
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.940