DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mhc and Myh4

DIOPT Version :9

Sequence 1:	NP_001162991.1	Gene:	Mhc / 35007	FlyBaseID:	FBgn0264695	Length:	1962	Species:	Drosophila melanogaster
Sequence 2:	NP_034985.2	Gene:	Myh4 / 17884	MGIID:	1339713	Length:	1939	Species:	Mus musculus

Alignment Length:	1922	Identity:	1071/1922 - (55%)
Similarity:	1432/1922 - (74%)	Gaps:	13/1922 - (0%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 DPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEGYLLGEIKATKGDIVSVGLQGGEVRDIKSEK 76
            :..|||..|.::|...|:||:|:|.|.::.|.||.|:...:::.:|..|:...:||....:|.::
Mouse    12 EAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESYVKATVQSREGGKVTAKTEGGATVTVKDDQ 76

  Fly    77 VEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAK 141
            |..:||||::||||||.||.|:.|.||:||::||.|.:||||||||||.:||||..|||......
Mouse    77 VFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVA 141

  Fly   142 MYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGAS--KK 204
            .||||:|.|.|||||:|||.||..|||:..|||:|||||||||||.|||:||.||||:..:  ||
Mouse   142 AYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKK 206

  Fly   205 TDEA--AKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLL 267
            .:||  .|.:|:||||::..||:|||||||||||||||||||||||||||.|||||.||||||||
Mouse   207 KEEATSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLL 271

  Fly   268 EKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTD 332
            ||:||..|...||||||||||||...|.:.::.|:|.|.||:..||||::||.||||.||...||
Mouse   272 EKSRVTFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMATD 336

  Fly   333 QAFDILGFTKQEKEDVYRITAAVMHMGGMKFKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKN 397
            .|.|||||:..||..:|::|.||||.|.|||||:.||||||.||.|...:.:.|...::|:|.|.
Mouse   337 TAVDILGFSADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKA 401

  Fly   398 LLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETLDTQQKRQHFIGVLDIA 462
            |..||:|||||:||:|:.||||.||:|||.|.:::::|.|:|.:.|:.|||:|.||:||||||||
Mouse   402 LCYPRVKVGNEYVTKGQTVQQVYNSVGALAKSMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIA 466

  Fly   463 GFEIFEYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYKREGIDWAFIDFGMDLLACIDLIEKPM 527
            |||||::|..|||||||||||||||||||||||||||||:|||||.||||||||.|||:||||||
Mouse   467 GFEIFDFNTLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIDWEFIDFGMDLAACIELIEKPM 531

  Fly   528 GILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITG 592
            ||.||||||.|||||||.:|..||...|||||..||||||.| |:..|||::.||||.|.|||.|
Mouse   532 GIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAK-GKAEAHFSLVHYAGTVDYNIIG 595

  Fly   593 WLEKNKDPLNDTVVDQFKKSQNKLLIEIFA-DHAGQSGGGEQAKGGRGKKGGGFATVSSAYKEQL 656
            ||:|||||||:|||..::||..|.|..:|: ..|.::.||...|||: |||..|.|||:.::|.|
Mouse   596 WLDKNKDPLNETVVGLYQKSGLKTLAFLFSGGQAAEAEGGGGKKGGK-KKGSSFQTVSALFRENL 659

  Fly   657 NSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKM 721
            |.|||.|:||.||||||:||||.|.||.::..||:|||.||||||||||||||||:|::|.|||.
Mouse   660 NKLMTNLKSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQ 724

  Fly   722 RYKIMCPKLL---QGVEKDKKATEIIIKFIDLPEDQYRLGNTKVFFRAGVLGQMEEFRDERLGKI 783
            |||::....:   |.:: .|||:|.::..||:...||:.|:|||||:||:||.:||.|||:|.::
Mouse   725 RYKVLNASAIPEGQFID-SKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQL 788

  Fly   784 MSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEI 848
            ::..||..||||.|..|||:.|:|.::..:|.|:|.::.::.|||.||:.|:||||..:..|.|:
Mouse   789 ITRTQAVCRGYLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEM 853

  Fly   849 ARLEEKAKKA-EELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKN 912
            |.::|..:|| |:|..:|.| |||||.....|:.||..|...:..|...|.|.:||..:|...|.
Mouse   854 ANMKEDFEKAKEDLAKSEAK-RKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCDQLIKTKI 917

  Fly   913 DLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNL 977
            .||.:::::.||...||:...:|..:|:|.:.|.|.|||||:||||.:.|.|::|...:::::||
Mouse   918 QLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNL 982

  Fly   978 NDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKK 1042
            .:|:|..||.|.||.||||...|.:|:|.::|||.|||:|.|.|.|.||||.:|:||.|||:|||
Mouse   983 TEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKK 1047

  Fly  1043 VRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQI 1107
            :|.|:|::|||:||||||.||:..|:|.:|::|::.:::|:.|:|::.:|:||||.:.::.|::|
Mouse  1048 LRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKI 1112

  Fly  1108 KELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSK 1172
            |||||||||||||:||||.:||||||||:||:|||||:.|||||||||||||||:|||||||..|
Mouse  1113 KELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQK 1177

  Fly  1173 LRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEKEKNEYYGQLNDLRAGVDHI 1237
            :|||||||.:|||:|.|.|||||.|:|||:.||:|.|.::|.|.||||:|...:::||.:.::.:
Mouse  1178 MRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETV 1242

  Fly  1238 TNEKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSK 1302
            :..|...||:.:.|:..|:||::|.:|..|.:|:....|.:|..|:.:..|||:|.::.|||||:
Mouse  1243 SKAKGNLEKMCRTLEDQLSEVKTKEEEQQRLINELSTQKARLHTESGEFSRQLDEKDAMVSQLSR 1307

  Fly  1303 IKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEA 1367
            .|.:.|.|:|:.||..:|||:.:..|....::..||.|.||||.|||.|.||:|||.:||||:|.
Mouse  1308 GKQAFTQQIEELKRQLEEESKAKNALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEV 1372

  Fly  1368 QVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVD 1432
            ..||:|||:|.:.|:|||||||:||..||.:|||.:|::|.||..||||||||..|||||.::|:
Mouse  1373 AQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVE 1437

  Fly  1433 RANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAV 1497
            |:||...|.:|||:.|||::.|||.|.::..|||:|||||.|:.|||||::|.||||..:|||.:
Mouse  1438 RSNAACAALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDQLETL 1502

  Fly  1498 RRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQL 1562
            :||||||..|:.||.:||.|||::|||:||.:|:::.||.||||:||||||:||.||.|:||.||
Mouse  1503 KRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQEKSELQASLEEAEASLEHEEGKILRIQL 1567

  Fly  1563 ELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIA 1627
            ||:||:.||||:|.||:||.:..::||.|.::|||::|:||.:.:.:|||:|||:|.|:||:||.
Mouse  1568 ELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQ 1632

  Fly  1628 LDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLE 1692
            |:|||:..|||.:|::..|..|||.|..|::..|.:||.:|||.:.|||||.:|.|:||.|..||
Mouse  1633 LNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQDDLKEQLAMVERRANLMQAEIEELRASLE 1697

  Fly  1693 QADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKA 1757
            |.:|.||.|||||.||.|::..:..||.|:...|:|||:::..:..::::::.||:|:|||||||
Mouse  1698 QTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKA 1762

  Fly  1758 MVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELE 1822
            :.|||.:|:||:.|||.:...|:::|.:||.:|:||.||||||..|||||||.|||||.||||||
Mouse  1763 ITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELE 1827

  Fly  1823 NELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEI 1887
            ||::.||:|:.:|.|.|||.|||||||::|:||||||..|:||||||||.|:|.||||.|||||.
Mouse  1828 NEVENEQKRNIEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAEEQ 1892

  Fly  1888 AALNLAKFRKAQQELEEAEERADLAEQAISKFRAKGR 1924
            :.:|||||||.|.||||||||||:||..::|.|.|.|
Mouse  1893 SNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSR 1929

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mhc	NP_001162991.1	Myosin_N	36..73	CDD:280832	9/36 (25%)
		MYSc	81..777	CDD:214580	443/703 (63%)
		MYSc_Myh1_insects_crustaceans	100..765	CDD:276874	423/672 (63%)
		Myosin_tail_1	842..1897	CDD:279860	559/1055 (53%)
		V_Alix_like	1557..1877	CDD:187408	174/319 (55%)
		ATP-synt_B	<1671..1771	CDD:304375	47/99 (47%)
		TMPIT	1739..>1825	CDD:285135	51/85 (60%)
Myh4	NP_034985.2	Myosin_N	35..73	CDD:308391	10/37 (27%)
		MYSc_Myh4	100..770	CDD:276879	423/672 (63%)
		Actin-binding. /evidence=ECO:0000250	659..681		16/21 (76%)
		Actin-binding. /evidence=ECO:0000250	761..775		8/13 (62%)
		SMC_N	850..1927	CDD:330553	576/1077 (53%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1128..1147		14/18 (78%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1153..1172		17/18 (94%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1276..1299		7/22 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	1105	1.000	Domainoid score	I20
eggNOG	1	0.900	-	-		E2759_KOG0161
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2092	1.000	Inparanoid score	I42
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41153at33208
OrthoFinder	1	1.000	-	-		FOG0000014
OrthoInspector	1	1.000	-	-		otm43784
orthoMCL	1	0.900	-	-		OOG6_100110
Panther	1	1.100	-	-	O	PTHR45615
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2660
SonicParanoid	1	1.000	-	-		X58
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	13	12.900