DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1D and Cacna1s

DIOPT Version :9

Sequence 1:	NP_723952.2	Gene:	Ca-alpha1D / 34950	FlyBaseID:	FBgn0001991	Length:	2705	Species:	Drosophila melanogaster
Sequence 2:	NP_446325.1	Gene:	Cacna1s / 682930	RGDID:	70983	Length:	1850	Species:	Rattus norvegicus

Alignment Length:	1936	Identity:	1016/1936 - (52%)
Similarity:	1280/1936 - (66%)	Gaps:	203/1936 - (10%)

- Green bases have known domain annotations that are detailed below.

  Fly   732 RQQRRGKPQPD---RPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPG 793
            |:::..||.|:   ||.||||||:::||||..||.:|||||||.:|||||||||:|||||.|.|.
  Rat    12 RKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISVVEWKPFETIILLTIFANCVALAVYLPMPE 76

  Fly   794 SDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQ 858
            .|:|..|..|||:||.||::|:.|..|||:||||:.|..||||:|||:|||.||.:|..:..|.|
  Rat    77 DDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTAILEQ 141

  Fly   859 L-----------MKDA-FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFV 911
            :           .|.| .||||||||||||||||||||||||||||||.|||:|||||||||||:
  Rat   142 VNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFM 206

  Fly   912 IIIYAIIGLELFSGKLHKAC----RDEITGEYEENIRPC---GVGYQCP-PGYKCYGGWDGPNDG 968
            :|||||||||||.||:||.|    .|.:.....|...||   |.|..|. .|.:|.|||.|||.|
  Rat   207 VIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHG 271

  Fly   969 ITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGE 1033
            ||:|||||.:||||:||:::||||||||.:.||:|::|.|:||:::::||:||::||:|||||||
  Rat   272 ITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGE 336

  Fly  1034 FSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAE--DIEPDAVGGLISDGKGKQPNEMDST 1096
            |:|||.|||:||.|||||||||:|||||||:.||||.|  |::....|.|..|..|.....:...
  Rat   337 FTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEI 401

  Fly  1097 ENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYG 1161
            |.|.:.:..::    .||:.       ||..|..|...|||:.||||:|::|.|||..:|:||:.
  Rat   402 EGLNKIIQFIR----HWRQW-------NRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHN 455

  Fly  1162 QLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMP 1226
            |..||.:.|:..|...:.|||.|||:|||.||.:.||:|:|||||||||...|.|.||..:|.|.
  Rat   456 QPLWLTHLQDVANRVLLALFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMT 520

  Fly  1227 PLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF 1291
            |||:|||||:||||:||:||||.|||||||||||||:||||||||||||::||||||||:|||::
  Rat   521 PLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFMIIFALLGMQLFGGRY 585

  Fly  1292 NFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGN 1356
            :|:..|.: |.|||.|.|||::|||::||||||:|||.||.||||.|..|.|.||||||||:|||
  Rat   586 DFEDTEVR-RSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPSYPGVLVCIYFIILFVCGN 649

  Fly  1357 YILLNVFLAIAVDNLADADSLSEVEK----------------EEEPHDESAQKKSHSPTPTIDGM 1405
            ||||||||||||||||:|:||:..:|                ::...:.|...|.....|..:|:
  Rat   650 YILLNVFLAIAVDNLAEAESLTSAQKAKAEERKRRKMSRGLPDKSEEERSTMTKKLEQKPKGEGI 714

  Fly  1406 DDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKILP 1470
            .....:.||..:..:       :|........:...::||.:.|...:.|||.::|:....|.:|
  Rat   715 PTTAKLKIDEFESNV-------NEVKDPYPSADFPGDDEEDEPEIPASPRPRPLAELQLKEKAVP 772

  Fly  1471 IPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDY 1535
            ||..:|||:||.||:.||.||.:.|.:.|.|.||..|:.|||.||||:|:||:...|::|..|||
  Rat   773 IPEASSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDY 837

  Fly  1536 FFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLRVL 1600
            .||||||:|::||:.:||..||.|:|||:.||:||||||.|||||:...|:||||||||||||||
  Rat   838 VFTAVFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVL 902

  Fly  1601 RPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNI 1665
            |||||||||||||||||||.||::|||||||||.|||||||            |
  Rat   903 RPLRAINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFA------------C----------- 944

  Fly  1666 MLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFH 1730
                         ||||||||||:.|.|.||||::||.|.|.:|.|||..:..||.|:|.:|.||
  Rat   945 -------------IGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFH 996

  Fly  1731 FDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFV 1795
            ||:|...|::|||||||||||.|||.:||||:|:.||::|.|..:|.::|||||:|||||:||||
  Rat   997 FDNVLSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFV 1061

  Fly  1796 GFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIFI 1860
            ||||||||.:||.|||||:||||||.|:::||||:|:|.||||:..||:||:.||||.|||.:|.
  Rat  1062 GFVIVTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFA 1126

  Fly  1861 LIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVL 1925
            |||:||:.|.|:.|||......:.|.||:.||.:|.||.:.||.||:.:.||||.||||||:||:
  Rat  1127 LIMLNTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMILKLIAFKPRGYFGDPWNVFDFLIVI 1191

  Fly  1926 GSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRT 1990
            ||.||::.|||.:...|......:..||.:......:. ||..||||||||||:||||:.||:||
  Rat  1192 GSIIDVILSEIDTLLASSGGLYCLGGGCGNVDPDESAR-ISSAFFRLFRVMRLIKLLSRAEGVRT 1255

  Fly  1991 LLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSA 2055
            ||||||||||||||||||||:|||||||:|||:|||||:..|..|..|||||||.||||:|||.|
  Rat  1256 LLWTFIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCA 1320

  Fly  2056 TGEAWQEIMMSCSAQPDVKCDMNSD-TPGE--PCGSSIAYPYFISFYVLCSFLIINLFVAVIMDN 2117
            ||||||||:::||.  ..:||..|| .|||  .||::.||.||||||:||:||||||||||||||
  Rat  1321 TGEAWQEILLACSY--GKRCDPESDYAPGEEYACGTNFAYYYFISFYMLCAFLIINLFVAVIMDN 1383

  Fly  2118 FDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRL 2182
            |||||||||||||||||||..:|:||||:|||||||||||||||:|.|||||||.||||:|||||
  Rat  1384 FDYLTRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRL 1448

  Fly  2183 VSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQVVPPP 2247
            |.|||||||||||.|||||||:|||:|.|||:||.:.||.||||.||:|||||:.||||||:||.
  Rat  1449 VGMNMPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPI 1513

  Fly  2248 GNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEGH-PDSNTVTLQAGLRTL-HEVSPALKRAIS 2310
            | ||||||||||||:|||::||:|.||  ||...|: |..:||.:||||||: .|.:|.:.||||
  Rat  1514 G-DDEVTVGKFYATFLIQEHFRKFMKR--QEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAIS 1575

  Fly  2311 GNL---DELDQE------PEPMHRRHHTLFGSVWSSIRRHGN----------------------- 2343
            |:|   :||::.      .|.:.||...|||.|.:.:.|..:
  Rat  1576 GDLTAEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEMEMEELES 1640

  Fly  2344 -----------GTFRRSAKATASQSNGALAIGGSASAALGVGGSSLVLGSSDPAGGDYLYDTLNR 2397
                       || ...|:|..:.:|..:|.|.|:.             .:.|......|:  ..
  Rat  1641 PVFLEDFPQNPGT-HPLARANTNNANANVAYGNSSH-------------RNSPVFSSIRYE--RE 1689

  Fly  2398 SVADGVNNITRNIMQARLAAAGKLQD--ELQGAGSGGELRTFGESISMRPLAKNGGGAATVAGTL 2460
            .:.:....:||.         |....  .:.|..|...:......:|.|.:.|         |.:
  Rat  1690 LLEEAGRPVTRE---------GPFSQPCSVSGVNSRSHVDKLERQMSQRRMPK---------GQV 1736

  Fly  2461 PPEANAINYDNRNRGILLHPYNNVYAPNGALPGHERMIQSTPASPYDQRRLPTSSDMNGLAES-- 2523
            ||....::.:.       ||.:.    .|  .|.......|..|...:.|:|.:|.....|.:
  Rat  1737 PPSPCQLSQEK-------HPVHE----EG--KGPRSWSTETSDSESFEERVPRNSAHKCTAPATT 1788

  Fly  2524 -LIGGVLAAEGLGKY-CDSEFVGTAAREMREALDMTPEEMNLAAHQILSNE 2572
             ||...|...||... .|:.||....:.:.:|..|.|||:.:||.::|..|
  Rat  1789 MLIQEALVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKRE 1839

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:395416	180/294 (61%)
		Ion_trans	1137..1379	CDD:395416	162/241 (67%)
		Ion_trans	1496..1809	CDD:395416	186/312 (60%)
		Ion_trans	1851..2126	CDD:395416	172/277 (62%)
		GPHH	2135..2188	CDD:407139	42/52 (81%)
		Ca_chan_IQ	2199..2271	CDD:400901	53/71 (75%)
Cacna1s	NP_446325.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..23		4/10 (40%)
		I. /evidence=ECO:0000305	38..337		182/298 (61%)
		Ion_trans	50..345	CDD:306908	180/294 (61%)
		Selectivity filter of repeat I. /evidence=ECO:0000250\|UniProtKB:P07293	290..293		0/2 (0%)
		Binding to the beta subunit. /evidence=ECO:0000250\|UniProtKB:Q02789	357..374		13/16 (81%)
		II. /evidence=ECO:0000305	418..664		162/246 (66%)
		Ion_trans	431..672	CDD:306908	162/241 (67%)
		Selectivity filter of repeat II. /evidence=ECO:0000250\|UniProtKB:P07293	612..615		2/2 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	673..717		5/43 (12%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	731..758		4/26 (15%)
		III. /evidence=ECO:0000305	786..1068		187/317 (59%)
		Ion_trans	798..1075	CDD:306908	186/312 (60%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	988..1077		58/88 (66%)
		Selectivity filter of repeat III. /evidence=ECO:0000250\|UniProtKB:P07293	1012..1015		2/2 (100%)
		IV. /evidence=ECO:0000305	1105..1384		170/281 (60%)
		Ion_trans	1117..1392	CDD:306908	172/277 (62%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250\|UniProtKB:P07293	1321..1324		2/2 (100%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1337..1403		51/65 (78%)
		Phenylalkylamine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1349..1391		32/41 (78%)
		GPHH	1393..1463	CDD:318993	59/69 (86%)
		Ca_chan_IQ	1465..1536	CDD:312334	53/71 (75%)
		Interaction with calmodulin. /evidence=ECO:0000250\|UniProtKB:Q13698	1522..1542		14/21 (67%)
		CAC1F_C	1558..1838	CDD:318975	69/326 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1697..1779		19/112 (17%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	383	1.000	Domainoid score	I788
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2028	1.000	Inparanoid score	I47
OMA	1	1.010	-	-		QHG48156
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	1	1.000	-	-		otm45290
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	1	1.100	-	-	O	PTHR45628
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X533
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.980