Protein Alignment Ca-alpha1D and Scn7a

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: NP_001375437.1
• Gene: Scn7a / 64155
• RGDID: 61922
• Length: 1680
• Species: Rattus norvegicus
• Alignment Length: 1758
• Identity: 424/1758 - (24%)
• Similarity: 742/1758 - (42%)
• Gaps: 395/1758 - (22%)

  Fly   747 ALFC-LSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVF 810
            ::.| ||..:.||...|:::....|..|||:::..:.|.:.:        ||:....| .||...
  Rat    96 SILCTLSPLSSLRRAVIKVLVHPLFRLLILISVLTDSILMCM--------SNLPEWIL-AVENTL 151

  Fly   811 LVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAI--STALSQLMKDAFDVKALRAFR 873
            |.|:|.|.::|::|.|....:.::|.:.||.|||::.:...|  |:.||.|..    .|.:|..|
  Rat   152 LGIYTFEILVKVIARGIWAGSFSFLGDLWNWLDFSVTLFELITRSSPLSSLPM----FKTIRTLR 212

  Fly   874 VLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKL-HKACR----- 932
            :|:.:.|..|:.|:.|.|...||.::....:|   ||.:.:.::.|:.||.|.| ||..|
  Rat   213 ILKIIPLNHGLQSIVVTLVQCLKKLLGAIALA---LFFLTVSSLFGMGLFMGNLKHKCVRWPQED 274

  Fly   933 -DEI----TGE-----------YEENIR---PCG----VGYQCPPGYKCYGGWDGPNDGITNFDN 974
             :::    ||.           |.|..|   .||    .|. ||.||.|......|::|.|:|||
  Rat   275 GNDVMYNGTGSQYHILERENFYYMEGARYALLCGNKTDAGL-CPEGYMCVKEGSNPDNGFTSFDN 338

  Fly   975 FGLAMLTVFQCVTLEGWTDVLY-SIQDAMGSDWQWMYFISMVILG---AFFVMNLILGVLSGEFS 1035
            ||.|:|.:|:.:| :.:.::|| .|..|.|.    :|.|..|::.   ||::.:|.||:|:..:.
  Rat   339 FGWALLAMFRLMT-QDYPELLYHQILYASGK----IYMIFFVLISFWFAFYMASLFLGILTMAYE 398

  Fly  1036 KERNKAKNRG---DFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTE 1097
            :|:.:|....   |.:..:..::.||:..|.....||.|             .|.:.|..:::|.
  Rat   399 QEKQRASEESRDMDSKCHQTVKEFEEEHEGAEMETTQIE-------------MKKRSPTSINTTL 450

  Fly  1098 NLGE-------EMPEVQMTESR------------------WRKMKKDFDRVNRRMRRACRKAVKS 1137
            ::.|       |.||....|..                  |.|:.:..||:           :..
  Rat   451 DILEDTALGHKEEPETSRKECPLCWYKFTKTCFIWKCSPCWIKLNEFADRI-----------ITH 504

  Fly  1138 QAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLF 1202
            ..|...:::.:.||...||.||:...:.|.:.....|:.|||::|.||:||:.::...|||...:
  Rat   505 PLFDLFLVICIILNICFLALEHFPMSEELMSLLAIGNLVFIGIYTIEMILKIIAMHPYGYFQISW 569

  Fly  1203 NRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIAS 1267
            :.||..:|:..:||.||.:..     .::|...|.|:.: |:.||.....||:..|..::.::..
  Rat   570 HIFDSILVVLGLTEMLLADIE-----EITVFILVPLIFI-KLGKYAPPFKNLMRILGRALVALKD 628

  Fly  1268 LLLLLFLFIVIFALLGMQVFGGKF-----NFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVM 1327
            |:||:.:||...|:.||::||..:     :.|...::.|.:...|..|.:|||:|:.|| |...:
  Rat   629 LVLLVSIFIYFSAVFGMKLFGRSYKDCVCHVDQDCQRQRWHMSDFLHAYVTVFRILCGE-WIETL 692

  Fly  1328 YVGINAYGGVSSYGALACI-YFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKE-------- 1383
            :..:..      .|...|| :::::.:.||.::|.:|:|: |.:.|..|:.:||.||
  Rat   693 WECMEV------AGEAWCIPFYMMVILIGNLLILYLFVAL-VSSFASYDATTEVSKEAKNLQLAV 750

  Fly  1384 ----------------------EEPHDESA---------------------------QKKSHSPT 1399
                                  .|..|::.                           |......|
  Rat   751 AWIKMVINCVLLKILCKEKTVSTEATDQTCDPSVKENISGHTLSELSNTQTFLRYKDQSSGTEKT 815

  Fly  1400 PTIDGMDDHL------SIDIDMEQQELDDEDKMDHET-------LSDEEVRE------------M 1439
            |..:.....|      |..:.:...|.|.|:..:.||       .|.|::::            :
  Rat   816 PVTESESQSLIASPSVSETVPIASGESDIENLDNKETRSKSANGSSKEKMKQSSSSECSTVDIAI 880

  Fly  1440 CEEEEEVDEEGMITARPRRMSEVNTATKILPIPPGTSFFLFSQTNR-------FRVFCHWLCNHS 1497
            .||||.|.|..............::|.::            |:.:|       .|..|..:..:|
  Rat   881 SEEEEMVYEHEKSKLHKNGYERKSSAGQV------------SRESRNGKIWRNIRKTCCKIVENS 933

  Fly  1498 NF----GNIILCCIMFSSAMLAAENPLRANDDLNKVLNKF----DYFFTAVFTIELILKLISYGF 1554
            .|    |.:.|.|    :..||.|:   ...|..|.:..|    |..|..:|.:|::||.::|||
  Rat   934 WFECFIGLVTLLC----TGTLALED---IYIDQRKTIKIFLEYGDMIFAYIFILEMLLKWVAYGF 991

  Fly  1555 VLHDGAFCRSAFNLLDLLVVCVSLISLVSSS----NAISVVKILRVLRVLRPLRAINRAKGLKHV 1615
                .|:..:.:..||.:||.|..:||:..:    |.::.:|.||.||||.
  Rat   992 ----KAYFSNNWYKLDFMVVIVLCLSLIGKTREDLNPLASIKFLRALRVLS-------------- 1038

  Fly  1616 VQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNIMLVTYLLQFMFAVIG 1680
                                 ||       :..|.|::.::.......::.||..::..:|:|:|
  Rat  1039 ---------------------QF-------ERMKVVLRALIKTTLPAVSVFLVCLMIWLLFSVMG 1075

  Fly  1681 VQLFKGKFFKCTDGSK---------MTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDVAK 1736
            |.||.|||::|.|.::         |.:.:|..  ||:::         ...|.|.:.:||:|..
  Rat  1076 VFLFAGKFYECIDPTRGERFSVFEVMNKSQCEN--LVFNE---------SMPWENAKLNFDNVGN 1129

  Fly  1737 GMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVT 1801
            |.|:||.|:||.||..::..:|||......|.......:..|:||:::...|..:.:.:|.:|..
  Rat  1130 GFLSLFQVATFNGWISIMNSAIDSVGVYMQPSFEHSLHMYTYFIIFVVFGLFLPLCMLIGVIIRN 1194

  Fly  1802 FQNEG-EQEYKNCDL---DKNQRNCIE---FALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIF 1859
            |..:. :|...|..:   .|.|...::   :|...||..|  |::..|..:...||...|...|.
  Rat  1195 FNKQKIKQGGSNIFITVKQKKQYRALKKLLYADSQKPAAR--PRNKFQGFICDVVTHRVFNVIII 1257

  Fly  1860 ILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIV 1924
            :||.....|:.::...|.......:..:|.:||.:|.||.:.||.|||. :||..||||.||::|
  Rat  1258 LLICFQATTIMIQNDEQSPQIETAVFWMNSLFTMLFTLECILKLTAFRC-HYFTSAWNVHDFMVV 1321

  Fly  1925 LGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEG-- 1987
            :.|...::                       ...|.|...:..:..:|..:.|::.:|..|:|
  Rat  1322 VFSITGLL-----------------------LPLSIGQYFVPPSLVQLLLLSRIIHVLRPGKGPK 1363

  Fly  1988 -IRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVL 2051
             ...|:...:.|..||..:||||.|:.||||:.||..|..:..:.|  |...:||:||..::|.|
  Rat  1364 VFHDLMLPLMLSLPALLNIALLIFLVMFIYAIFGMYNFAYVKKEAG--INDVSNFETFGSSMLCL 1426

  Fly  2052 FRSATGEAWQEIMMSCSAQPDVKCDMNSDTPGE----PCGS-SIAYPYFISFYVLCSFLIINLFV 2111
            |:..|...|..::.:........||.:...||.    .||| |:...||:|:.::...:|:|::|
  Rat  1427 FQVTTFSGWDGMLDAIFNSQWSDCDPDKINPGTQVRGDCGSPSVGIFYFVSYILISWLIIVNMYV 1491

  Fly  2112 AVIMDNFDYLT----RDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTL---LRKISPPLGF 2169
            .:||   ::|:    |....|.......|.::|:.:|||   |.:::|...|   ...:.|||..
  Rat  1492 VLIM---EFLSIPSKRKNRTLSEDDFRRFFKVWNRFDPD---RTQYIDSTKLSDFAAALDPPLFM 1550

  Fly  2170 GKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKR 2234
            .|  |::   .:||:|::|:.:...:.....|.|..:..:.  .|..::...||    |:..:..
  Rat  1551 AK--PNK---GQLVAMDLPMAAGDRIHCLDILLAFTKRVMG--KDERVEKILSE----IESGFML 1604

  Fly  2235 TNP-KLLDQVVPPPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQ-------EGKEGHPDSNTVTL 2291
            .|| |:..:.:.......:..|.   || :||..::.::.|:..       |..:|..|.|:..:
  Rat  1605 ANPFKITYEPITTTLKRKQEAVS---AT-IIQRAYKSYRLRQSDKKIQDIPEIDDGREDPNSKGV 1665

  Fly  2292 QAG 2294
            .:|
  Rat  1666 HSG 1668

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	90/309 (29%)
		Ion_trans	1137..1379	CDD:459842	68/247 (28%)
		Ion_trans	1496..1809	CDD:459842	82/334 (25%)
		Ion_trans	1851..2126	CDD:459842	79/286 (28%)
		GPHH	2135..2188	CDD:465306	16/55 (29%)
		Ca_chan_IQ	2198..2273	CDD:462591	14/75 (19%)
Scn7a	NP_001375437.1	I. /evidence=ECO:0000250|UniProtKB:Q01118	100..401		94/322 (29%)
		Ion_trans	117..405	CDD:459842	90/309 (29%)
		II. /evidence=ECO:0000250|UniProtKB:Q01118	487..756		76/293 (26%)
		Ion_trans	504..738	CDD:459842	68/247 (28%)
		Na_trans_assoc	742..926	CDD:461936	25/195 (13%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	807..874		12/66 (18%)
		III. /evidence=ECO:0000250|UniProtKB:Q01118	915..1223		88/371 (24%)
		Ion_trans	932..1203	CDD:459842	82/334 (25%)
		Na_channel_gate	1194..1245	CDD:240441	11/52 (21%)
		IV. /evidence=ECO:0000250|UniProtKB:Q01118	1232..1530		91/331 (27%)
		Ion_trans	1249..1496	CDD:459842	77/275 (28%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1646..1680		5/23 (22%)