Protein Alignment Ca-alpha1D and scn8aa

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: XP_068072894.1
• Gene: scn8aa / 58152
• ZFINID: ZDB-GENE-000828-1
• Length: 1960
• Species: Danio rerio
• Alignment Length: 2004
• Identity: 482/2004 - (24%)
• Similarity: 826/2004 - (41%)
• Gaps: 600/2004 - (29%)

  Fly   747 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFL 811
            ||:.:|..|..|.:.|:|:....|...|:.||..||:.:....|...|         ::|||.|.
Zfish   111 ALYMISPFNLARRIAIKILIHSVFSMFIMCTILTNCVFMTFSNPPEWS---------KQVEYTFT 166

  Fly   812 VIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLR 876
            .|:|.|..:||:|.||.:....:||:.||.|||.:     ||.|......|..:|.|||.|||||
Zfish   167 GIYTFESAVKIIARGFCIDGFTFLRDPWNWLDFMV-----ISMAYVTEFVDLGNVSALRTFRVLR 226

  Fly   877 PLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC---------- 931
            .|:.:|.:|.|:.::.::::::..|..:.:|.:|.:.::|:|||:||.|.|.:.|
Zfish   227 ALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVIWPINITEM 291

  Fly   932 RDEITG-------EY---EEN--IRP-------CGVGY---QCPPGYKCYGGWDGPNDGITNFDN 974
            ..::.|       ||   |.|  ..|       ||...   :||.||.|......||.|.|:||:
Zfish   292 FPDVNGSRAFNWDEYILNETNFYFLPDQLDALLCGNSSDSGRCPEGYTCMKAGRNPNYGYTSFDS 356

  Fly   975 FGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGEFSKERN 1039
            ||.|.|.:|:.:|.:.|.::......|.|..:. ::|:.::.:|:|:::||||.|::..: :|:|
Zfish   357 FGWAFLALFRLMTQDFWENLYQLTLRAAGKTYM-IFFVLVIFVGSFYLVNLILAVVAMAY-EEQN 419

  Fly  1040 KAKNRGDFQKLREKQQIEEDLRGYLDWI-TQAEDIEPDAV---GGLIS--------DGKGK---Q 1089
            :|       .:.|.::.|.:.:..|:.: .|.||.:.:|:   .|.:|        ||:|.   .
Zfish   420 QA-------TMEEAERKEAEFKAMLEQLKKQQEDAQANAMATSAGTVSEDVVEDDGDGEGNLSCS 477

  Fly  1090 PNEMDSTENLGEEMPEVQMTESRWRKMKKDFD-------------------------RVNRR--- 1126
            .:||....:  :...|.:..:.:||:.::|.:                         |:.||
Zfish   478 SSEMSKLSS--KSAKERRNRKKKWRQKEQDKEKGDSEKFVKSESDDGSRRRFRFPDNRLGRRSSI 540

  Fly  1127 --------------MRRACRKAVKSQ--------------------------------------A 1139
                          .||..:.::.|:                                      .
Zfish   541 MNQSLLSIPGSPFPSRRNSKSSIFSRCKDGGSENEFADDEHSTVEEYDERRDSFLSPQRRSSYTG 605

  Fly  1140 FY--------------------------------------------------------------- 1141
            ||
Zfish   606 FYGKRNSTVDCNGVVSLIGPGPGGRLLPEVIIDKAATDDSPTTDLEIKKKLSGSLMVSVEQLNTS 670

  Fly  1142 ------------------------------------------------WL--------------- 1143
                                                            |:
Zfish   671 FGRKERANSVMSALTNTLVEELEESQRKCPPCWYKFANTFLIWECGPLWMSIKEIVNLIVMDPFV 735

  Fly  1144 ---IIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRF 1205
               |.:.:.|||..:|.|||......::.....|:.|.|:||.||..|:.::....||...:|.|
Zfish   736 DLAITICIVLNTLFMAMEHYPMTPQFEHVLSVGNLVFTGIFTAEMFAKLVAMDPYYYFQEGWNIF 800

  Fly  1206 DCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLL 1270
            |.|:|..|:.|..|.|..     |:||||..|||||||:.|.|.:|:.|:..:.||:.::.:|.|
Zfish   801 DGFIVSLSLMELGLANVE-----GLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTL 860

  Fly  1271 LLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDC---------FWQALLTVFQIMTGEDWNAV 1326
            :|.:.:.|||::|||:||..:    |:...::..||         |:.:.|.||:::.|| |...
Zfish   861 VLAIIVFIFAVVGMQLFGKSY----KDCVCKIAQDCELPRWHMNDFFHSFLIVFRVLCGE-WIET 920

  Fly  1327 MYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAI-----AVDNLADADSLSE------- 1379
            |:..:...|     .|:..|.|:::.:.||.::||:|||:     :.||||.:|...|
Zfish   921 MWDCMEVAG-----QAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAASDDDGEMNNLQIA 980

  Fly  1380 --------------------------VEKEEEPHDESAQKKSHSPTPTIDGMDDHLSIDI--DME 1416
                                      |..|.:|.|:...:|       ::.:.:|..:||  |::
Zfish   981 VIRIKKGIAWVKAKVRELVNIILGRKVTDEAKPLDDMYDRK-------LNCIANHTGVDISRDLD 1038

  Fly  1417 QQE----------------LDDEDKM-------------------DHETLSDEEVREMCEEE--E 1444
            .|:                :.|:|.|                   |.|.|:.|:.....|.|  :
Zfish  1039 YQKNGNGTTSGIGSSVGKYMIDDDHMSFIHNPNLTVCVPIAVGESDFENLNTEDFSSESEAEGSK 1103

  Fly  1445 EVDE----EG-MITARPRRMSEVNTATKI-------------------------LPIPPGTS--- 1476
            |:|:    || .|..:|    ||..|..:                         :||..|..
Zfish  1104 ELDDISSSEGSTIDIKP----EVEEAVVVETVEEYVDPEACWTEACIARYKCCDVPITEGWGKNW 1164

  Fly  1477 FFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENP-LRANDDLNKVLNKFDYFFTAV 1540
            :||       |..|:.:..|:.|..:|:..|:.||..||.|:. :.....:..:|...|..||.:
Zfish  1165 WFL-------RKTCYLIVEHNWFETLIIFMILLSSGALAFEDVYIEQRKTIRIILEYADMVFTYI 1222

  Fly  1541 FTIELILKLISYGFVLH-DGAFCRSAFNLLDLLVVCVSLISLVSSS---NAISVVKILRVLRVLR 1601
            |.:|::||.::||||.: ..|:|     .||..:|.||::||::::   :.:..:|.||.||.||
Zfish  1223 FILEMLLKWVAYGFVKYFTNAWC-----WLDFFIVDVSIVSLIANALGYSDLGPIKSLRTLRALR 1282

  Fly  1602 PLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNIM 1666
            ||||::|.:|::.||..::.|:.:|.|::|| ||:.::
Zfish  1283 PLRALSRFEGMRVVVNALVGAIPSIMNVLLV-CLIFWL--------------------------- 1319

  Fly  1667 LVTYLLQFMFAVIGVQLFKGKFFKCTDGSKM---------TQDECYGTYLVYDDGDVHKPRLRER 1722
                    :|:::||.:|.||::.|.:.::.         .:.||:.         :.:....|.
Zfish  1320 --------IFSIMGVNMFAGKYYYCYNETEKAYFELDVVNNKSECFA---------LIEQNYTEV 1367

  Fly  1723 EWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIA 1787
            .|.|.:.:||:|..|.|.|..|:||:||..::|.::||.:....|.:.....:..|::|:||..:
Zfish  1368 RWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRRVEDQPKYEDNIYMYIYFVIFIIFGS 1432

  Fly  1788 FFMVNIFVGFVIVTFQNE----GEQEYKNCDLDKNQRNCIEFALK-------AKPVRRYIPKHGI 1841
            ||.:|:|:|.:|..|..:    |.|:....:..|...|    |:|       .||:.|  |::.:
Zfish  1433 FFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYN----AMKKLGSKKPQKPIPR--PQNKL 1491

  Fly  1842 QYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAF 1906
            |..|:.|||...|:.:|.|||.:|.||:.::..:|......:|..:|.||...|..|||.||.|.
Zfish  1492 QGMVFDFVTQQVFDISIMILICLNMVTMMVETDDQSQETENILYWINFIFIVAFTSEFVLKLFAL 1556

  Fly  1907 RFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFR 1971
            | ..||.:.||:||.::|:.|.:.:..:             |::|     |......|..:  .|
Zfish  1557 R-HYYFTNGWNIFDCVVVILSIVGMFLA-------------DLIE-----KYFVSPTLFRV--IR 1600

  Fly  1972 LFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAIT 2036
            |.|:.|:::|:...:||||||:..:.|..||..:.||:.|:.||:::.||..|..:..:.|  |.
Zfish  1601 LARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKRESG--ID 1663

  Fly  2037 ANNNFQTFQQAVLVLFRSATGEAWQEIMMS-CSAQPDVKCDMNSDTPGEP----CGSSIAYPYFI 2096
            ...||:||..:::.||...|...|..::.. .:..||  |:...:.||..    ||:.....:|.
Zfish  1664 DMYNFETFGNSMICLFMITTSAGWDGLLAPILNYPPD--CEPTKENPGTSVKGNCGNPSVGIFFF 1726

  Fly  2097 SFYVLCSFLI-INLFVAVIMDNFDYLTRDWS-ILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTL 2159
            ..|::.|||| :|:::|:|::||...|.:.: .|.....:.|..:|.::||.|...|....:...
Zfish  1727 VMYIIVSFLIVVNMYIAIILENFSVATEESADPLCEDDFESFYEIWEKFDPTASQFITFAKLGDF 1791

  Fly  2160 LRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSEL 2224
            ...:..||...|  |:.:   .|::|::|:.|...:.....|||..:..|.  ..|::|    .:
Zfish  1792 ADTLEHPLRVPK--PNTI---ELIAMDLPMVSGDRIHCLDILFAFTKRVLG--DSGDLD----MM 1845

  Fly  2225 RATIKQIWKRTNPKLLDQVVPPP------GNDDEVTVGKFYATYLIQDYFRRFKKRK-------- 2275
            |..:::.:...||   .:|...|      ...:.::.|      :||..||....||
Zfish  1846 RQQMEERFIAANP---SKVSFEPITTTLRRKQEHMSAG------VIQRAFRAHLIRKGFICKRLL 1901

  Fly  2276 ----------EQEGKEGHPDSNTVTLQAGLRTLHEVSPALKRAISGNLDELDQE 2319
                      .|:.|||.|.:      |.|.:...|:...|.    .|:|.|.:
Zfish  1902 SSSRLENGGTNQDKKEGTPST------ASLPSYDSVTKPEKE----KLEESDSK 1945

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	94/306 (31%)
		Ion_trans	1137..1379	CDD:459842	87/422 (21%)
		Ion_trans	1496..1809	CDD:459842	91/330 (28%)
		Ion_trans	1851..2126	CDD:459842	84/280 (30%)
		GPHH	2135..2188	CDD:465306	12/52 (23%)
		Ca_chan_IQ	2198..2273	CDD:462591	16/80 (20%)
scn8aa	XP_068072894.1	Ion_trans	131..423	CDD:459842	95/314 (30%)
		ARGLU	<416..>448	CDD:405931	9/38 (24%)
		Na_trans_cytopl	544..682	CDD:463401	5/137 (4%)
		Ion_trans	732..945	CDD:459842	71/227 (31%)
		Na_trans_assoc	971..1172	CDD:461936	35/218 (16%)
		Ion_trans	1177..1453	CDD:459842	90/325 (28%)
		Na_channel_gate	1445..1497	CDD:240441	13/57 (23%)
		Ion_trans	1502..1756	CDD:459842	84/278 (30%)