DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1D and Cacna1f

DIOPT Version :9

Sequence 1:	NP_723952.2	Gene:	Ca-alpha1D / 34950	FlyBaseID:	FBgn0001991	Length:	2705	Species:	Drosophila melanogaster
Sequence 2:	NP_062528.2	Gene:	Cacna1f / 54652	MGIID:	1859639	Length:	1984	Species:	Mus musculus

Alignment Length:	2074	Identity:	1068/2074 - (51%)
Similarity:	1307/2074 - (63%)	Gaps:	323/2074 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly   703 PETMNGTTIGP---------------SGAGGQKGGAAAGAAGQKRQQRRGK----PQPDRPQRAL 748
            |...|||..||               |||.|      .|...::.|..:.|    ....|..|||
Mouse    14 PSPANGTGPGPEWGLCPGPPTVGTDTSGASG------LGTPRRRTQHNKHKTVAVASAQRSPRAL 72

  Fly   749 FCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVI 813
            |||::.||:|..||.|||||||:.||||||||||:||.||.|:|..|||..|..||:||||||||
Mouse    73 FCLTLTNPIRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVI 137

  Fly   814 FTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQ---LMKDA---------FDV 866
            ||.|.|:||:|||.|||..||:|||||||||.|||:|..|..|.|   ...||         |||
Mouse   138 FTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDV 202

  Fly   867 KALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC 931
            |||||||||||||||||||||.:|||||:||:|||.|||||||||||||||||||||.|::||.|
Mouse   203 KALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTC 267

  Fly   932 RDEITG---EYEENIRPC---GVGYQCPPGY-KCYGGWDGPNDGITNFDNFGLAMLTVFQCVTLE 989
              ...|   |.||:..||   |.|..|...: :|.|.|.|||.||||||||..||||||||:|:|
Mouse   268 --YFLGSDMEAEEDPSPCASSGSGRSCTLNHTECRGRWPGPNGGITNFDNFFFAMLTVFQCITME 330

  Fly   990 GWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGEFSKERNKAKNRGDFQKLREKQ 1054
            |||||||.:|||||.:..|:||:|:||.|:|||:||:||||||||||||.|||.|||||||||||
Mouse   331 GWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQ 395

  Fly  1055 QIEEDLRGYLDWITQAEDI---EPDAVGGLIS---DGK-GKQP--NEMDS--------------- 1095
            |:|||||||||||||||::   :|...|.|.|   :|: |.:|  :|:.:
Mouse   396 QMEEDLRGYLDWITQAEELDLHDPSVDGNLASLAEEGRAGHRPQLSELTNRRRGRLRWFSHSTRS 460

  Fly  1096 ------------------TENLGEEMPEVQMTESRWRKMKK--------DFDRVNRRMRRACRKA 1134
                              |:..|:|..|.....|..|.:.|        .|.|.||.:|..||:|
Mouse   461 THSTSSHASLPASDTGSMTDTPGDEDEEEGTMASCTRCLNKIMKTRICRHFRRANRGLRARCRRA 525

  Fly  1135 VKSQAFYWLIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFV 1199
            |||.|.||.:::||||||..:|:||:||..||...|||.|...:.|||.|||||:|.||...|..
Mouse   526 VKSNACYWAVLLLVFLNTLTIASEHHGQPLWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSVYVA 590

  Fly  1200 SLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQS 1264
            |.|||||||||.|.|.||.|...|.|.|||:||||||||||:||||::|.||||||||||||::|
Mouse   591 SFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKS 655

  Fly  1265 IASLLLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYV 1329
            ||||||||||||:||:|||||:||||||||....| |..||.|.|||||||||:||||||.|||.
Mouse   656 IASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTK-RSTFDTFPQALLTVFQILTGEDWNVVMYD 719

  Fly  1330 GINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEEEPHDESAQKK 1394
            ||.||||....|.|.|:||||||||||||||||||||||||||..|:.:..:|..|...|....|
Mouse   720 GIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDAGTAKDKGREKSSEGNPPK 784

  Fly  1395 SHSPTPTIDGMDDHLSIDIDMEQQEL----DDEDKMDHET------LSDEEVREMCEEEEEVDEE 1449
                                 |.:.|    ::||.....:      :.:||..|..|||||.:|.
Mouse   785 ---------------------ENKVLVPGGENEDAKGARSEGAAPGMEEEEEEEEEEEEEEEEEN 828

  Fly  1450 GMITARPRRMSEVNTATKILPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAML 1514
            |  ......:.||....|::|||.|::||..||||..|..||.|.:|..|.::||..|:.||..|
Mouse   829 G--AGHVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRKACHTLIHHHIFTSLILVFIILSSVSL 891

  Fly  1515 AAENPLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLI 1579
            |||:|:||:...|.:|..|||.||::||:|::||:..:|..||.|:||||.|||||||||.||||
Mouse   892 AAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNLLDLLVVSVSLI 956

  Fly  1580 SLVSSSNAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIG 1644
            |....|:|||||||||||||||||||||||||||||||||.
Mouse   957 SFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVF------------------------ 997

  Fly  1645 VQLFKYVVKCVVVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVY 1709
                        |||:||||||:||.|||||||.||||||||||:.|||.:|.|..||.|::|:|
Mouse   998 ------------VAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTLKECKGSFLIY 1050

  Fly  1710 DDGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPI 1774
            .||||.:|.:|||.|.|:.|:||:|...|:.|||||||||||.|||.:||:|.|:.|||:|:...
Mouse  1051 PDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDANAEDEGPIYNYHVE 1115

  Fly  1775 VAAYYIIYIIIIAFFMVNIFVGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKH 1839
            ::.::|:|||||||||:||||||||:||:.:|||||:||:||||||.|:|:||||:|:||||||:
Mouse  1116 ISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKN 1180

  Fly  1840 GIQYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLA 1904
            ..||:||..|.|::|||.:|:||::|||.|||:.|.|...:...:|.|||:||.:|.:|.|.|:.
Mouse  1181 PHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKII 1245

  Fly  1905 AFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINF 1969
            ||:.|:||.||||.||.:||:||.:||..:|:.:             |....:.|..|:.|||.|
Mouse  1246 AFKPKHYFADAWNTFDALIVVGSVVDIAVTEVNN-------------GGHLGESSEDSSRISITF 1297

  Fly  1970 FRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNA 2034
            ||||||||||||||||||||||||||||||||||||||||.::||||||:|||:|||:||..|..
Mouse  1298 FRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQ 1362

  Fly  2035 ITANNNFQTFQQAVLVLFRSATGEAWQEIMMSCSAQPDVKCDMNSD-TPGE--PCGSSIAYPYFI 2096
            |..|||||||.||||:|||.||||||||||:  ::.|..:||..|| .|||  .||||.|..|||
Mouse  1363 INRNNNFQTFPQAVLLLFRCATGEAWQEIML--ASLPGNRCDPESDFGPGEEFTCGSSFAIVYFI 1425

  Fly  2097 SFYVLCSFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLR 2161
            ||::||:|||||||||||||||||||||||||||||||||.|:||||||.|||||||||||.|||
Mouse  1426 SFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLR 1490

  Fly  2162 KISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRA 2226
            :|.||||||||||||:||||||:||:||||||||.|||||||:|||||.|||:||:|.||.|||.
Mouse  1491 RIQPPLGFGKLCPHRVACKRLVAMNVPLNSDGTVTFNATLFALVRTSLKIKTEGNLDQANQELRM 1555

  Fly  2227 TIKQIWKRTNPKLLDQVVPPPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQE--GKEGHPDSNTV 2289
            .||:||||...||||:|:||| :::||||||||||:|||||||:|::|||:.  |:|. |.|.:.
Mouse  1556 VIKKIWKRIKQKLLDEVIPPP-DEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGREA-PTSTSS 1618

  Fly  2290 TLQAGLRTLHEVSPALKRAISGNLDELDQE----------------PEPMHRRHHTLFGSVWSSI 2338
            .||||||:|.::.|.:::|::.:.:|.::|                |||......:...|.|:|
Mouse  1619 ALQAGLRSLQDLGPEIRQALTYDTEEEEEEEEAVGQEAEEEEAENNPEPYKDSIDSQPQSRWNS- 1682

  Fly  2339 RRHGNGTFRRSAKATASQS-NGALAIGGSASAALGVGG--SSLVLGSSDP---AGGDYLY----- 2392
                    |.|......:. ..:|:.|.|....|....  .|::...|.|   :.|.:::
Mouse  1683 --------RISVSLPVKEKLPDSLSTGPSDDDGLAPNSRQPSVIQAGSQPHRRSSGVFMFTIPEE 1739

  Fly  2393 -----------DTLN--RSVADGVNNIT-----------------------RNIMQARL---AAA 2418
                       |..|  :.|.|...::.                       .::.:.||   ..|
Mouse  1740 GSIQLKGTQGQDNQNEEQEVPDWTPDLDEQAGTPSNPVLLPPHWSQQHVNGHHVPRRRLLPPTPA 1804

  Fly  2419 GKLQDE----LQGAGSGGEL---RTFGESISMRPLAKNGGGAATVAGTLPPEANAINYDNRNRGI 2476
            |:....    ||..||..:|   .|:....:..|....|..||      ||:          :|.
Mouse  1805 GRKPSFTIQCLQRQGSCEDLPIPGTYHRGRTSGPSRAQGSWAA------PPQ----------KGR 1853

  Fly  2477 LLHPYNNVYAP-------------NGALPGHER---MIQSTPASPYDQRRLPTSSDMNGLAESLI 2525
            ||      |||             .|.|.|..|   .:|...|.|....|      ..|.|:||:
Mouse  1854 LL------YAPLLLVEESTVGEGYLGKLGGPLRTFTCLQVPGAHPNPSHR------KRGSADSLV 1906

  Fly  2526 GGVLAAEGLGKYC-DSEFVGTAAREMREALDMTPEEMNLAAHQIL--------SNEHSL 2575
            ..||.:||||.:. |..||..|.:|:.:|..:|.:||:.||..:|        |:|.|:
Mouse  1907 EAVLISEGLGLFAQDPRFVALAKQEIADACHLTLDEMDSAASDLLAQRTTSLYSDEESI 1965

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:395416	195/293 (67%)
		Ion_trans	1137..1379	CDD:395416	167/241 (69%)
		Ion_trans	1496..1809	CDD:395416	179/312 (57%)
		Ion_trans	1851..2126	CDD:395416	171/277 (62%)
		GPHH	2135..2188	CDD:407139	44/52 (85%)
		Ca_chan_IQ	2199..2271	CDD:400901	52/71 (73%)
Cacna1f	NP_062528.2	Ion_trans	109..383	CDD:278921	180/275 (65%)
		Ion_trans	546..763	CDD:278921	155/217 (71%)
		Ion_trans	891..1110	CDD:278921	148/254 (58%)
		Ion_trans	1210..1455	CDD:278921	161/259 (62%)
		GPHH	1463..1526	CDD:293510	53/62 (85%)
		Ca_chan_IQ	1528..1599	CDD:285917	52/71 (73%)
		CAC1F_C	1623..1981	CDD:293490	83/380 (22%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	383	1.000	Domainoid score	I823
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2021	1.000	Inparanoid score	I52
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48156
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	1	1.000	-	-		otm43218
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	1	1.100	-	-	O	PTHR45628
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X533
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.980