DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1D and cacna1da

DIOPT Version :9

Sequence 1:	NP_723952.2	Gene:	Ca-alpha1D / 34950	FlyBaseID:	FBgn0001991	Length:	2705	Species:	Drosophila melanogaster
Sequence 2:	XP_021335251.1	Gene:	cacna1da / 403310	ZFINID:	ZDB-GENE-030616-135	Length:	2093	Species:	Danio rerio

Alignment Length:	2151	Identity:	1111/2151 - (51%)
Similarity:	1341/2151 - (62%)	Gaps:	397/2151 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly   700 ANIPETMNGTTIGPSGAGGQKGGAA-AGAAGQKRQ------QRRGKPQPDRPQRALFCLSVKNPL 757
            ||.|.....|...|..|........ .|:..||::      :::|.....|||||||||::.||:
Zfish     3 ANGPAPPAATPAAPPAAAVPVPSVVPVGSLAQKKRAQYAKSKKQGSSANTRPQRALFCLNLNNPI 67

  Fly   758 RALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKI 822
            |..||.:||||||:..||:.|||||:|||||.|:|..|||.||..||.|||.||:|||.|..:||
Zfish    68 RRACISLVEWKPFDIFILIAIFANCMALAVYVPFPEDDSNSTNHDLETVEYAFLIIFTIETFLKI 132

  Fly   823 LAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKD---------------------AFDV 866
            :|||.|:|..||:|||||:|||.|||||..|..|..|.|:                     .|||
Zfish   133 IAYGLVMHQNAYVRNGWNMLDFVIVVIGLFSVVLEVLTKEGGEKEEVGENLSAHGHGGKPGGFDV 197

  Fly   867 KALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC 931
            ||||||||||||||||||||||||||||:||||||.|||||||||||||||||||||.||:|.:|
Zfish   198 KALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHASC 262

  Fly   932 RDEITGEYEENIRPCGV---GYQCP-PGYKCYGGWDGPNDGITNFDNFGLAMLTVFQCVTLEGWT 992
            ..:.|...|:...||.|   |..|| .|..|..||.|||.||||||||..||||||||:|:||||
Zfish   263 YFQGTDILEDEPAPCAVNGHGRTCPINGTLCKEGWQGPNGGITNFDNFMFAMLTVFQCITMEGWT 327

  Fly   993 DVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGEFSKERNKAKNRGDFQKLREKQQIE 1057
            ||||.:.||||.:..|:||:|:||.|:|||:||:||||||||||||.|||.||||||||||||:|
Zfish   328 DVLYWMNDAMGLELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLE 392

  Fly  1058 EDLRGYLDWITQAEDIEPD-------------AVGGLI-------------SDGKGKQP---NEM 1093
            |||:||||||||||||:|:             .:..|:             ||.....|   .|.
Zfish   393 EDLKGYLDWITQAEDIDPENEEEEEESKRNRVTLASLMEKKKKGFGWFSQSSDTHASMPASETES 457

  Fly  1094 DSTEN-LGEEM------PEVQMTES-----RWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIV 1146
            .:||| .||:.      |..|....     |||       |.||..||.||.||||..||||:|:
Zfish   458 MNTENEKGEDEKATCCGPTCQKISKSKFSRRWR-------RWNRLCRRNCRLAVKSVPFYWLVII 515

  Fly  1147 LVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVI 1211
            ||||||..:::|||.|..||...|:..|...:.:||||||:||||||.|.||||||||||||||.
Zfish   516 LVFLNTLTISSEHYNQPMWLTQVQDVANKVLLAMFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVC 580

  Fly  1212 GSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFI 1276
            |.||||:|....:|.|||:||.|||||||:||||::|.||||||||||||::|||||||||||||
Zfish   581 GGITETILVEFEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFI 645

  Fly  1277 VIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYG 1341
            :||:||||||||||||||..:.| |..||.|.|||||||||:||||||||||.||.||||.||.|
Zfish   646 IIFSLLGMQVFGGKFNFDETQTK-RSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSG 709

  Fly  1342 ALACIYFIILFICGNYILLNVFLAIAVDNLADADSLS--EVEKEEEPHDESAQKKSHSPTPTIDG 1404
            .:.||||||||||||||||||||||||||||||:||:  :.:|.:|
Zfish   710 MIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTDDTKKPDE------------------- 755

  Fly  1405 MDDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPR-RMSEVNTATKI 1468
                    ||    |::||.|...|   ||:     :..||.:||..:.|.|| ::||:....||
Zfish   756 --------ID----EIEDEAKAGEE---DEK-----DNAEEDEEEPDVPAGPRPKISELVKKEKI 800

  Fly  1469 LPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKF 1533
            .|||.|::||:||.||..||.||.|.||..|.|:||..||.|||.||||:|:|.....|.:|..|
Zfish   801 TPIPEGSAFFIFSNTNPVRVACHKLINHHIFTNLILVFIMLSSASLAAEDPIRNFSARNIILGYF 865

  Fly  1534 DYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLR 1598
            ||.|||:||:|::||:.:||..||.|||||:.|||||||||.|||:|....|:||||||||||||
Zfish   866 DYAFTAIFTVEIVLKMTTYGAFLHKGAFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLR 930

  Fly  1599 VLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIG 1663
            |||||||||||||||||||||.                                    |||:|||
Zfish   931 VLRPLRAINRAKGLKHVVQCVF------------------------------------VAIRTIG 959

  Fly  1664 NIMLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNR 1728
            |||:||.|||||||.||||||||||::|.|.:|.:.:||.|||::|.:|||::|.:::|.|.|:.
Zfish   960 NIMIVTTLLQFMFACIGVQLFKGKFYRCNDEAKSSPEECKGTYIMYKEGDVNQPIIQKRHWHNSD 1024

  Fly  1729 FHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNI 1793
            |:||:|...|:.|||||||||||.|||.:||||:||.|||:|:|..::.::|||||||||||:||
Zfish  1025 FNFDNVLMAMMALFTVSTFEGWPALLYKAIDSNRENMGPIYNYRVEISIFFIIYIIIIAFFMMNI 1089

  Fly  1794 FVGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTI 1858
            ||||||||||.:||:|||||:||||||.|:|:||||:|:||||||:..|||.|:.|.|:.|||.:
Zfish  1090 FVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIM 1154

  Fly  1859 FILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFII 1923
            |:||::||:.||::.|.|...:..::|.|||:|||||.:|.|.||.||:.::||.||||.||.:|
Zfish  1155 FVLILLNTICLAVQHYGQSELFNYVMDILNMVFTAVFTVEMVLKLIAFKPRHYFTDAWNTFDALI 1219

  Fly  1924 VLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGI 1988
            |:||.:||..:|:...:..|:.|..      :|:.||   .|||.|||||||||||||||:||||
Zfish  1220 VVGSVVDIAITEVNPTEAPQVDESG------NTEDSA---RISITFFRLFRVMRLVKLLSRGEGI 1275

  Fly  1989 RTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFR 2053
            |||||||||||||||||||||.:|||||||:|||||||||:.....|..|||||||.||||:|||
Zfish  1276 RTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKIAMVDHTQINRNNNFQTFPQAVLLLFR 1340

  Fly  2054 SATGEAWQEIMMSCSAQPDVKCDMNSD-TPGE--PCGSSIAYPYFISFYVLCSFLIINLFVAVIM 2115
            .||||||||||::|  .|...||..|| .|||  .||||.|..|||:||:||:||||||||||||
Zfish  1341 CATGEAWQEIMLAC--MPGKLCDPESDYNPGEEMTCGSSFAIIYFITFYMLCAFLIINLFVAVIM 1403

  Fly  2116 DNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACK 2180
            |||||||||||||||||||||.|:||||||:|||||||||||||||:|.||||||||||||:|||
Zfish  1404 DNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACK 1468

  Fly  2181 RLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQVVP 2245
            |||:|||||||||||:|||||||:|||:|.|||:||::.||.||||.||:|||||:.||||||||
Zfish  1469 RLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKRTSMKLLDQVVP 1533

  Fly  2246 PPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEG-HPDSN--TVTLQAGLRTLHEVSPALKR 2307
            |.| ||||||||||||:|||||||:|||||| ||..| ||..|  .:.|||||||||::.|.::|
Zfish  1534 PAG-DDEVTVGKFYATFLIQDYFRKFKKRKE-EGLVGVHPAQNNTAIALQAGLRTLHDIGPEIRR 1596

  Fly  2308 AISGNL--DEL----DQEPEPMHRRHHTLFGSVWSSI----RR---------------------- 2340
            |||.:|  |||    .:|.|.::||:..|||:..:.|    ||
Zfish  1597 AISCDLQDDELVDFIPEEDEEIYRRNGGLFGNHINHINGDPRRSSGHQTNATQRPLQVQPPPHYV 1661

  Fly  2341 -------------------------------HGNGTFRRSAKATASQSNGA----LAIGG----- 2365
                                           |.|.::.:|.|:|....|.|    |..||
Zfish  1662 HMEQPVGRLGRANAMAQQNHHRHHHHHHHHHHHNNSYNKSPKSTNINLNNANVSSLPNGGHNRYY 1726

  Fly  2366 SASAALGVGGSSLVLGSSDPAGGDY------------LYDTLNRSVADGVNNITRNIMQARLAAA 2418
            ..:.|.|..||..         |:|            .|:|..||  .|.:.....|.:.....
Zfish  1727 EHAPANGYPGSYY---------GEYDKPRTPHGQRRRYYETYIRS--QGSDRRRPTIRREEEYE- 1779

  Fly  2419 GKLQDELQGAGSGGELRTFGESISM------------------------------RPLAKNGGGA 2453
               :|...|....||  .|.|..||                              :|| .:....
Zfish  1780 ---EDRYSGEYYSGE--EFYEDDSMLSGDRYPNSDQEYETPRGYHHPDSYYEDDEQPL-YHDSHR 1838

  Fly  2454 ATVAGTLP--PEAN---AINYD------------NRNRGILLH---------------------- 2479
            :.....||  |:.|   :.|::            ::...:.||
Zfish  1839 SPKRRLLPPTPQGNRRPSFNFECLRRQSSQDDLPHQRTALPLHLMQHQVMAVAGLDSSRAHRLSP 1903

  Fly  2480 --------------------PYNNVYAP---------NGALPGHERMIQS---TPASPYDQR--- 2509
                                ||   |.|         :.|...|..:.:|   |....|.||
Zfish  1904 TRSTRSWASPPPTPASKDRTPY---YTPLIRVDRPLRDSASSSHSSIRKSSWYTDDPEYQQRNFS 1965

  Fly  2510 ----RLPTS-----SDMNGLAESLIGGVLAAEGLGKYC-DSEFVGTAAREMREALDMTPEEMNLA 2564
                ::|..     ....|.|.||:..||.:||||:|. |.:||.....|:.:|.:||.:||..|
Zfish  1966 PVHLQVPPEYRNQYLQKRGSATSLVEAVLISEGLGRYAKDPKFVAATKHEIADACEMTIDEMESA 2030

  Fly  2565 AHQILS 2570
            |..:|:
Zfish  2031 ASHLLN 2036

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:395416	191/299 (64%)
		Ion_trans	1137..1379	CDD:395416	177/243 (73%)
		Ion_trans	1496..1809	CDD:395416	184/312 (59%)
		Ion_trans	1851..2126	CDD:395416	174/277 (63%)
		GPHH	2135..2188	CDD:407139	45/52 (87%)
		Ca_chan_IQ	2199..2271	CDD:400901	56/71 (79%)
cacna1da	XP_021335251.1	Ion_trans	77..377	CDD:306908	191/299 (64%)
		Ion_trans	509..744	CDD:306908	174/235 (74%)
		Ion_trans	828..1105	CDD:306908	184/312 (59%)
		Ion_trans	1150..1414	CDD:306908	173/274 (63%)
		GPHH	1415..1485	CDD:318993	62/69 (90%)
		Ca_chan_IQ	1487..1558	CDD:312334	56/71 (79%)
		CAC1F_C	1583..2092	CDD:318975	99/475 (21%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	376	1.000	Domainoid score	I823
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H578
Inparanoid	1	1.050	1984	1.000	Inparanoid score	I53
OMA	1	1.010	-	-		QHG48156
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	1	1.000	-	-		otm25328
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	1	1.100	-	-	LDO	PTHR45628
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X533
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.980