Protein Alignment Ca-alpha1D and scn1laa

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: NP_956426.2
• Gene: scn1laa / 393101
• ZFINID: ZDB-GENE-040426-751
• Length: 1955
• Species: Danio rerio
• Alignment Length: 1940
• Identity: 495/1940 - (25%)
• Similarity: 807/1940 - (41%)
• Gaps: 556/1940 - (28%)

  Fly   747 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFL 811
            ||:..|..|.||.:.|:|:....|.|:|:.||.||| |..|||..|....|        |||||.
Zfish   108 ALYIFSSFNLLRRISIQILVHFLFNFIIICTILANC-AFMVYTQTPDWAKN--------VEYVFT 163

  Fly   812 VIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLR 876
            .|:|.|.::||.|.||.:....:||:.||.|||.::|:..::..:     |...|.|||||||||
Zfish   164 GIYTLEALVKITARGFCVGRFTFLRDPWNWLDFIVIVMAYVTEFV-----DLGSVSALRAFRVLR 223

  Fly   877 PLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKL-------------- 927
            .|:.:|.||.|:.::.::::::..|..:.:|.||.:.::|::||:||.|.|
Zfish   224 ALKTISVVPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALVGLQLFMGILKQKCVYSTSFTENK 288

  Fly   928 -----HKACRDEITGEYEENIRP-------CG----VGYQCPPGYKCYGGWDGPNDGITNFDNFG 976
                 :|.|||.|.....:...|       ||    .| |||.||.|......|:.|.|:||:||
Zfish   289 EACYKNKTCRDYINNPSNQYFLPGKKDALICGNSSDAG-QCPEGYTCLKAGKNPDYGYTSFDSFG 352

  Fly   977 LAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGV------------ 1029
            .|.|::|:.:|.:.|.::......|.|..:. ::|:.::.||:|:::||||.|
Zfish   353 WAFLSLFRLMTQDFWENLYQQTLRAAGKTYM-IFFVLVIFLGSFYLVNLILAVVAMAYDEQNQAT 416

  Fly  1030 ------------------------------------LSGE--------------FSKERNKAKNR 1044
                                                ||||              .:|||...|.:
Zfish   417 IDEALKKEEEFNAMLEQITRQEQEAEAAAEQSKCEGLSGEKTDSSSDASKLSSKSAKERRNRKKK 481

  Fly  1045 GDFQKLREKQQIEEDLRGYLDWITQAEDIEPD---AVGGLI------------------------ 1082
            ...|...||::         |.|.:..|.:|:   ||.|.|
Zfish   482 RQHQGESEKEE---------DSIKEGNDRKPNFTFAVDGKIRRSRDRSYTSPRQSPLSFQGSLLT 537

  Fly  1083 --SDGKG----------KQPNEMDSTENLGEE--------------------------------- 1102
              .|.|.          ...|..|..:::.||
Zfish   538 PRRDSKASVFSFRGRIHSDNNYADDEQSMFEETGSRRDSLFLPTRPERLFSNVSKSSLTPHLLLP 602

  Fly  1103 --------------------------------MPEVQM-------------TESRWRKMKKDFDR 1122
                                            .|||..             .|.|.|...::|.:
Zfish   603 SNGKARYVDDSNGMVMVTSGASAPNSPVIALNTPEVSRKRAMFDDTCADSDDERRSRCNSQNFSK 667

  Fly  1123 VNRRMRR--------------ACRKAVKSQAFYW------------------------------- 1142
            ..|:..|              .|.:..|.:...|
Zfish   668 AYRQSMRERALSVASGSNNPAECSEIFKQRCEEWWFEFAENYLIWDCSPTWLRIKEVVHMIVMDP 732

  Fly  1143 ----LIIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFN 1203
                .|.:.:.:||..:|.|.|.......:.....||.|||:||.||:.|:.:|....||:..:|
Zfish   733 FADLFITICIVINTVFMAMECYPMDGPYSSMLSTANVVFIGIFTAEMIFKVIALDPYSYFLEGWN 797

  Fly  1204 RFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASL 1268
            .||..:|..|:.|..|.|.    ..|:..||..|||||||:.|.|.:|:.|:..:.||:.::::|
Zfish   798 IFDSIIVTLSLMELCLANV----LSGIGFLRPFRLLRVFKLAKSWPTLNMLIKIIGNSLGALSNL 858

  Fly  1269 LLLLFLFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDC---------FWQALLTVFQIMTGEDWN 1324
            .|:|.:.:.|||::|||:||..:.    |...:::.||         |:.:.|.||:::.|| |.
Zfish   859 TLVLAIIVFIFAVVGMQLFGKSYG----ECVCKISSDCMLPRWHMNDFFHSFLIVFRVLCGE-WI 918

  Fly  1325 AVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEEEPHDE 1389
            ..|:..:...|     ..|....|:::.:.||.::||:|||:.:.:.: ||:|:...:|:|.:.:
Zfish   919 ETMWDCMEVAG-----QPLCLTVFMMVMVIGNLVVLNLFLALLLSSFS-ADNLAVSGEEDENNLQ 977

  Fly  1390 SA-----------------------------------------QKKSHSPTPTID------GMD- 1406
            .|                                         ::|..:...||:      |.|
Zfish   978 IAVRRIRKGFTFIKTCLQSFFRSGCLRKKKQKSADGNMIVNCKERKDSTNHTTIELVKSTGGTDL 1042

  Fly  1407 ----------DHLSIDIDMEQQE-----LDDED--------KMDHETLSD--------------- 1433
                      ..|::.:.:..:|     |:.||        :.|.|.|||
Zfish  1043 DSDTENFISNPSLTVSVPIAAEESDFECLNTEDFSSLSSDLEEDKEKLSDVASSFSEGSTVDLRP 1107

  Fly  1434 -----EEVREMCEEEEEVDEEGMITA---RPRRMSEVNTATKILPIPPGTSFFLFSQTNRFRVFC 1490
                 |:..|:..|||...|. ..||   |..|..:|:....|     ...::|..||      |
Zfish  1108 LGKDGEDALELVLEEEAYPEP-CFTAGCVRRFRCCQVDEEKGI-----WKKWWLLRQT------C 1160

  Fly  1491 HWLCNHSNFGNIILCCIMFSSAMLAAEN-PLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGF 1554
            :.:..||.|...|:..|:.||..||.|: .|.....:..:|...|..||.:|.:|::||.::|||
Zfish  1161 YNIVEHSWFETFIVFMILLSSGALAFEDIYLDTRKSVKTILEFADKVFTYIFILEMLLKWVAYGF 1225

  Fly  1555 VLH-DGAFCRSAFNLLDLLVVCVSLISLVSSS---NAISVVKILRVLRVLRPLRAINRAKGLKHV 1615
            ..: ...:|     .||.::|.|||||||:::   :.:|.:|.||.||..|||||::|..|::.|
Zfish  1226 AKYFTNLWC-----WLDFVIVDVSLISLVANALNYSELSAIKSLRTLRAFRPLRALSRFDGMRVV 1285

  Fly  1616 VQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNIMLVTYLLQFMFAVIG 1680
            |..::.|:.:|.|::|| ||:.::                                   :|:::|
Zfish  1286 VNALLGAIPSIMNVLLV-CLIFWL-----------------------------------IFSIVG 1314

  Fly  1681 VQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVS 1745
            |..|.|||.:|.:   :|.::......||:..|..:.....| |.|...:||:|..|.|.|..|:
Zfish  1315 VNFFAGKFSECIN---ITTEQRLPVSEVYNKTDCERMGKGVR-WRNVIINFDNVGNGYLALLQVA 1375

  Fly  1746 TFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNE----G 1806
            ||:||..::|.::||......|.:.....:..|::|:||:.|||.:|:|:|.:|..|..:    |
Zfish  1376 TFKGWMPIMYAAVDSRNPEEQPEYEVNLYMYLYFVIFIILGAFFTLNLFIGVIIDNFNQQKKKFG 1440

  Fly  1807 EQEYKNCDLDKNQRNCIEFALK-------AKPVRRYIPKHGIQYKVWWFVTSSSFEYTIFILIMI 1864
            .|:....:..|...|    |:|       .||:.|  ||:.||..::..||..:|:..|.:||.:
Zfish  1441 GQDIFMTEEQKKYYN----AMKKLGSKKPQKPIPR--PKNKIQGLIFDLVTKQAFDIFIMVLIWL 1499

  Fly  1865 NTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFI 1929
            |.||:.::..:|.:..|..|..:|:||..:|..|.|.|:.|.| ..:|...|||||||:|:.|.:
Zfish  1500 NMVTMMLETEDQSIEMTRALYWINVIFIVLFTTECVLKMIALR-HYFFTVGWNVFDFIVVILSIV 1563

  Fly  1930 DIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFF---RLFRVMRLVKLLSKGEGIRTL 1991
            .:..:|:..|                       ..:|...|   ||.|:.|:::|:...:|||||
Zfish  1564 GMSLTEVIKK-----------------------YFVSPTLFRVIRLARIGRVLRLIRSAKGIRTL 1605

  Fly  1992 LWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSAT 2056
            |:..:.|..||..:.||:.|:.||||:.||..|..:....|  |....||:||..::|.||:..|
Zfish  1606 LFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKTAG--IDDMFNFETFPNSMLCLFQITT 1668

  Fly  2057 GEAWQEIM--MSCSAQPDVKCDMNSDTPGE----PCGS-SIAYPYFISFYVLCSFLIINLFVAVI 2114
            ...|..::  |..|..||  ||...:..|.    .||: ||...:|:|:.::|..:::|:::|||
Zfish  1669 SGGWDGLLAPMLNSKPPD--CDPTKNFTGSSVVGDCGNPSIGIFFFVSYIIICFLIVVNMYIAVI 1731

  Fly  2115 MDNFDYLTRDWS-ILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMA 2178
            ::||...|.:.: .|.....|.|..:|.::||.|...:::..:......:.|||...|  |:|.
Zfish  1732 LENFSVATEESAEPLSEDDFDRFYEVWEKFDPKATQFMEYGKLSDFADSLDPPLRIPK--PNRF- 1793

  Fly  2179 CKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQV 2243
              :|:||::|:.:...:.....|||..:..|.  ..|.:|..:.::.....|    :||.
Zfish  1794 --QLMSMDLPMVTGDRIHCLDILFAFTKRVLG--EGGEMDMLHGQMEDRFMQ----SNPS----- 1845

  Fly  2244 VPPPGNDDEVT--------------VGKFYATYLIQDYF---RRFKKR-KEQEGK 2280
               ..:.:.:|              :.:.:..:|::|..   |.|||: |.|.||
Zfish  1846 ---KSSYEPITTTLRRKHEDMSAAVIQRAFRKHLVRDSMKKVRNFKKKNKHQNGK 1897

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	107/366 (29%)
		Ion_trans	1137..1379	CDD:459842	79/285 (28%)
		Ion_trans	1496..1809	CDD:459842	98/321 (31%)
		Ion_trans	1851..2126	CDD:459842	89/284 (31%)
		GPHH	2135..2188	CDD:465306	14/52 (27%)
		Ca_chan_IQ	2198..2273	CDD:462591	14/91 (15%)
scn1laa	NP_956426.2	Ion_trans	128..416	CDD:459842	100/303 (33%)
		Na_trans_cytopl	527..682	CDD:463401	15/154 (10%)
		Ion_trans	731..945	CDD:459842	68/227 (30%)
		Na_trans_assoc	974..1161	CDD:461936	31/198 (16%)
		Ion_trans	1166..1438	CDD:459842	97/316 (31%)
		Na_channel_gate	1430..1482	CDD:240441	14/57 (25%)
		Ion_trans	1487..1742	CDD:459842	89/282 (32%)
		GPHH	1753..1801	CDD:465306	14/52 (27%)