Protein Alignment Ca-alpha1D and Scn8a

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: XP_017172001.1
• Gene: Scn8a / 20273
• MGIID: 103169
• Length: 1988
• Species: Mus musculus
• Alignment Length: 2003
• Identity: 488/2003 - (24%)
• Similarity: 845/2003 - (42%)
• Gaps: 559/2003 - (27%)

  Fly   747 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFL 811
            ||:.||..|.:|.:.|:|:....|..:|:.||..||:.:....|...|         :.|||.|.
Mouse   111 ALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWS---------KNVEYTFT 166

  Fly   812 VIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLR 876
            .|:|.|.::||:|.||.:....:||:.||.|||:::::..::..:     |..:|.|||.|||||
Mouse   167 GIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFV-----DLGNVSALRTFRVLR 226

  Fly   877 PLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC---------- 931
            .|:.:|.:|.|:.::.::::::..|..:.:|.:|.:.::|:|||:||.|.|...|
Mouse   227 ALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVVWPINFNES 291

  Fly   932 ------RDEITGEYEEN----------IRP--CG----VGYQCPPGYKCYGGWDGPNDGITNFDN 974
                  |.....||..|          :.|  ||    .| |||.|::|......||.|.|:||.
Mouse   292 YLENGTRGFDWEEYINNKTNFYMVPGMLEPLLCGNSSDAG-QCPEGFQCMKAGRNPNYGYTSFDT 355

  Fly   975 FGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVL--------- 1030
            |..|.|.:|:.:|.:.|.::......|.|..:. ::|:.::.:|:|:::||||.|:
Mouse   356 FSWAFLALFRLMTQDYWENLYQLTLRAAGKTYM-IFFVLVIFVGSFYLVNLILAVVAMAYEEQNQ 419

  Fly  1031 ---------------------------------------------------------SGEFSKER 1038
                                                                     |.|.||..
Mouse   420 ATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEDGVGSPRSSSELSKLS 484

  Fly  1039 NK-AKNRGDFQKLREKQQIEE-DLRGYLDWITQAEDIE---------PD-AVG--------GLIS 1083
            :| ||.|.:.:|.|:::::.| :.:|..:.:.::|..:         || .:|        .|:|
Mouse   485 SKSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLS 549

  Fly  1084 ---------------------DGKGKQP---NEMDSTE--------------------------- 1097
                                 .|:.:.|   ||....|
Mouse   550 IPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSY 614

  Fly  1098 ------------------------------------------NLGEEMPEVQM---------TES 1111
                                                      ::|..:|||::         ||.
Mouse   615 SGYSGYSQCSRSSRIFPSLRRSVKRNSTVDCNGVVSLIGPGSHIGRLLPEVKIDKAATDSATTEV 679

  Fly  1112 RWRKM----------------KKDFDRVNRRMRRACRKAVK----------------SQAF---- 1140
            ..:|.                :|  ||:|..|.......|:                :..|
Mouse   680 EIKKKGPGSLLVSMEQLASYGRK--DRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWE 742

  Fly  1141 ---YWL------------------IIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCE 1184
               ||:                  |.:.:.|||..:|.||:......::.....|:.|.|:||.|
Mouse   743 CHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAE 807

  Fly  1185 MLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWR 1249
            |.||:.::....||...:|.||.|:|..|:.|     .|:....|:||||..|||||||:.|.|.
Mouse   808 MFLKLIAMDPYYYFQEGWNIFDGFIVSLSLME-----LGLADVEGLSVLRSFRLLRVFKLAKSWP 867

  Fly  1250 SLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGG-------KFNFDGKEEKYRMNFDCF 1307
            :|:.|:..:.||:.::.:|.|:|.:.:.|||::|||:||.       |.:.:.|..::.||  .|
Mouse   868 TLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISQECKLPRWHMN--DF 930

  Fly  1308 WQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAI-----A 1367
            :.:.|.||:::.|| |...|:..:...|     .|:..|.|:::.:.||.::||:|||:     :
Mouse   931 FHSFLIVFRVLCGE-WIETMWDCMEVAG-----QAMCLIVFMMVMVIGNLVVLNLFLALLLSSFS 989

  Fly  1368 VDNLADAD----------SLSEVEK-----------------------EEEPHDESAQKKSH--- 1396
            .||||..|          |:..::|                       |.:|.||..:||::
Mouse   990 ADNLAATDDDGEMNNLQISVIRIKKGVAWAKVKVHAFMQAHFKQREADEVKPLDELYEKKANCIA 1054

  Fly  1397 ---------------SPTPTIDGM----------DDHLSI------------------------- 1411
                           :...|..|:          :||:|.
Mouse  1055 NHTGVDIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTE 1119

  Fly  1412 DIDMEQQELDDEDKMDHETLSDE--------EVREMCEE--EEEVDEEGMIT---ARPRRMSEVN 1463
            |:..|......:||:| :|.|.|        ||.|:..|  ||.:|.:...|   .:..:..:||
Mouse  1120 DVSSESDPEGSKDKLD-DTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVN 1183

  Fly  1464 TATKILPIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAEN-PLRANDDLN 1527
            ....:     |.|:::..:|      |..:..|:.|...|:..|:.||..||.|: .:.....:.
Mouse  1184 IEEGL-----GKSWWILRKT------CFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIR 1237

  Fly  1528 KVLNKFDYFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSS---NAIS 1589
            .:|...|..||.:|.:|::||..:||||    .|..:|:..||.|:|.|||:||::::   :.:.
Mouse  1238 TILEYADKVFTYIFILEMLLKWTAYGFV----KFFTNAWCWLDFLIVAVSLVSLIANALGYSELG 1298

  Fly  1590 VVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKC 1654
            .:|.||.||.||||||::|.:|::.||..::.|:.:|.|::|| ||:.::
Mouse  1299 AIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLV-CLIFWL--------------- 1347

  Fly  1655 VVVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFKC---TDGSKMTQDECYGTYLVYDDGDVHK 1716
                                :|:::||.||.||:..|   |...:...||      |.:..|..|
Mouse  1348 --------------------IFSIMGVNLFAGKYHYCFNETSEIRFEIDE------VNNKTDCEK 1386

  Fly  1717 ---PRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAY 1778
               ....|..|.|.:.:||:|..|.|.|..|:||:||..::|.::||.|.:..|.:.....:..|
Mouse  1387 LMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPDYEGNIYMYIY 1451

  Fly  1779 YIIYIIIIAFFMVNIFVGFVIVTFQNE----GEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPK- 1838
            ::|:||..:||.:|:|:|.:|..|..:    |.|:....:..|...|.:: .|.:|..::.||:
Mouse  1452 FVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMK-KLGSKKPQKPIPRP 1515

  Fly  1839 -HGIQYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFK 1902
             :.||..|:.|||..:|:..|.:||.:|.||:.::...|......:|..:|::|...|..|.|.|
Mouse  1516 LNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLK 1580

  Fly  1903 LAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISI 1967
            :.|.| ..||...||:|||::|:.|.:.:..:             ||:|     |......|..:
Mouse  1581 MFALR-HYYFTIGWNIFDFVVVILSIVGMFLA-------------DIIE-----KYFVSPTLFRV 1626

  Fly  1968 NFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGG 2032
              .||.|:.|:::|:...:||||||:..:.|..||..:.||:.|:.||:::.||..|..:..:.|
Mouse  1627 --IRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAG 1689

  Fly  2033 NAITANNNFQTFQQAVLVLFRSATGEAWQEIMMSCSAQPDVKCDMNSDTPGE----PCGS-SIAY 2092
              |....||:||..:::.||:..|...|..:::....:|. .|.::.:.||.    .||: |:..
Mouse  1690 --IDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPP-DCSLDKEHPGSGFKGDCGNPSVGI 1751

  Fly  2093 PYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWS-ILGPHHLDEFIRLWSEYDPDAKGRIKHLDV 2156
            .:|:|:.::...:::|:::|:|::||...|.:.: .|.....:.|..:|.::||||...|::..:
Mouse  1752 FFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKL 1816

  Fly  2157 VTLLRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDAN 2221
            ......:..||...|  |:.:   .|::|::|:.|...:.....|||..:..|.  ..|.:|
Mouse  1817 ADFADALEHPLRVPK--PNTI---ELIAMDLPMVSGDRIHCLDILFAFTKRVLG--DSGELD--- 1871

  Fly  2222 SELRATIKQIWKRTNPKLL--DQVVPPPGNDDE----VTVGKFYATYLIQDYF--RRFKKRKEQE 2278
             .||..:::.:..:||..:  :.:........|    |.:.:.|..:|.:..|  |:....|.:.
Mouse  1872 -ILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICRKITSNKLEN 1935

  Fly  2279 G------KEGHPDSNTVTLQAGLRTLHEVSPALK----RAISGNLDELDQEPE 2321
            |      ||..|.:      |.|.:...|:...|    ||..|..:...::.|
Mouse  1936 GGTHREKKESTPST------ASLPSYDSVTKPDKEKQQRAEEGRRERAKRQKE 1982

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	93/373 (25%)
		Ion_trans	1137..1379	CDD:459842	87/288 (30%)
		Ion_trans	1496..1809	CDD:459842	99/326 (30%)
		Ion_trans	1851..2126	CDD:459842	78/279 (28%)
		GPHH	2135..2188	CDD:465306	13/52 (25%)
		Ca_chan_IQ	2198..2273	CDD:462591	16/82 (20%)
Scn8a	XP_017172001.1	Ion_trans	131..421	CDD:459842	88/305 (29%)
		Na_trans_cytopl	546..710	CDD:463401	18/165 (11%)
		Ion_trans	760..973	CDD:459842	71/225 (32%)
		Na_trans_assoc	999..1200	CDD:461936	34/212 (16%)
		Ion_trans	1205..1481	CDD:459842	98/321 (31%)
		Na_channel_gate	1473..1525	CDD:240441	12/52 (23%)
		Ion_trans	1530..1784	CDD:459842	78/277 (28%)
		IQ	1902..1923	CDD:197470	4/20 (20%)