Protein Alignment Ca-alpha1D and Scn7a

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: NP_033161.2
• Gene: Scn7a / 20272
• MGIID: 102965
• Length: 1681
• Species: Mus musculus
• Alignment Length: 1768
• Identity: 434/1768 - (24%)
• Similarity: 750/1768 - (42%)
• Gaps: 390/1768 - (22%)

  Fly   747 ALFC-LSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVF 810
            ::|| ||..|.||...|:.:....|..|||:::..:.|.:.:        ||:....| .:|...
Mouse    97 SIFCTLSPLNSLRRAAIKALVHPLFRLLILISVLTDSILMCM--------SNLPEWIL-AIENTL 152

  Fly   811 LVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAIS--TALSQLMKDAFDVKALRAFR 873
            |.|:..|.::|::|.|....:.::|.:.||.|||::.:...|:  :.||..:.    :|.:|.||
Mouse   153 LGIYAFEILVKVIARGIWAGSFSFLGDLWNWLDFSVTLFELITRFSPLSSFLM----LKTIRTFR 213

  Fly   874 VLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKL-HKACR----- 932
            :|:.:.|..|:.|:.:.|...||   .||....|.||.:.:::::|:.||.|.| ||..|
Mouse   214 ILKIIPLNHGLQSIVMTLAQCLK---KLFGAIALALFFLAVFSLLGMGLFMGNLKHKCLRWPEEN 275

  Fly   933 ----------------DEITGEYEENIR---PCG----VGYQCPPGYKCYGGWDGPNDGITNFDN 974
                            :.|...|.|..:   .||    .| |||.||.|......|::|.|:|||
Mouse   276 ENETLHNRTGSLNYSPERINFYYMEGAKYALLCGNRTDAG-QCPEGYVCVKEGTNPDNGFTSFDN 339

  Fly   975 FGLAMLTVFQCVTLEGWTDVLY-SIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGEFSKER 1038
            ||.::|.:|:.:| :.:.::|| .|..|.|..:. ::|:.:....||::.:|.||:|:..:.||:
Mouse   340 FGWSLLAMFRLMT-QDYPELLYHQILYASGKVYM-IFFVMISFWFAFYLTSLFLGILTMTYEKEK 402

  Fly  1039 NKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTENLGE-- 1101
            .:|.........:.:|.::|     ||....|.::|...:     :.|.:.|..:::|.::.|
Mouse   403 QRACEESGGLDPKCQQTVKE-----LDEENDAAEMETTQI-----EMKKRSPTSINTTLDILEDT 457

  Fly  1102 -----EMPEVQMTESR------------------WRKMKKDFDRVNRRMRRACRKAVKSQAFYWL 1143
                 |.||....:..                  |.|:.:..|||          .....|..:|
Mouse   458 TLGHREEPETSRKKCPICWHKFIKTCFIWKCSPCWVKLNEFADRV----------ITHPLADLFL 512

  Fly  1144 IIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCF 1208
            :|.:| ||...||.||:...:.|.:.....|:.|||::|.||:||:.::...|||...:|.||..
Mouse   513 VICIV-LNICFLALEHFPMSEELRSLLHVGNLVFIGIYTIEMILKIIAMHPYGYFQISWNIFDSI 576

  Fly  1209 VVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLF 1273
            :|:..:||.||.:..     |::||..|.|:.: |:.||.....:|:..|.:|:.::..|:|||.
Mouse   577 LVVLELTEILLADVE-----GLAVLITVPLIFI-KLGKYGPPFKSLMRILGSSLMALKDLVLLLC 635

  Fly  1274 LFIVIFALLGMQVFGGKFNFDGKEEKYRMNFDC---------FWQALLTVFQIMTGEDWNAVMYV 1329
            :|:...|:.||::||..:    |:....:..||         |..|.:|||:|:.|| |...::.
Mouse   636 IFVYFSAVFGMKLFGRSY----KDCVCHIKEDCQPQRWHMSDFLHAYMTVFRILCGE-WIETLWE 695

  Fly  1330 GINAYGGVSSYGALACI-YFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKE---------- 1383
            .:..      .|...|| :::::.:.||.::|.:|:.: |.:.:..|:.|||.||
Mouse   696 CMEV------AGQAWCIPFYMMVILIGNLLILYLFVTL-VSSFSYYDATSEVNKEAKNLQLAMAR 753

  Fly  1384 --------------------EEPHDE---------------------------SAQKKSHSPTPT 1401
                                .|..|:                           ..|..|...||.
Mouse   754 IKSGINSMLLKLMCTERSVPTEATDQICDPSVKENISGHTLSELSNTQTFLRYKDQSSSTEKTPV 818

  Fly  1402 IDGMDDHL------SIDIDMEQQELDDEDKMDHETL-------SDEEVRE------------MCE 1441
            .:.....|      |..:.:...|.|.|:..:.||.       |.|::::            :.|
Mouse   819 TESESQSLIASPSASETVPIASGESDIENLDNKETRSKSGNGGSKEKMKQSSSSECSTVDIAISE 883

  Fly  1442 EEEEVDEE----------------GMITARPRRMSEV-----NTATKILPIPPGTSFFLFSQTNR 1485
            |||.|.|.                |.| :|..|..::     .|..||:            :.:.
Mouse   884 EEEMVYEHEKSKLHKNGYERKSSTGQI-SRESRNGKIWKNIRKTCCKIV------------ENSW 935

  Fly  1486 FRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKF-DYFFTAVFTIELILKL 1549
            |..|         .|.:.|.|    :..||.|:.........|:|.:: |..|..:|.:|::||.
Mouse   936 FECF---------IGLVTLLC----TGTLALEDIYIDQRKTTKILLEYADMIFAYIFILEMLLKW 987

  Fly  1550 ISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSS----NAISVVKILRVLRVLRPLRAINRAK 1610
            ::|||    .||..:.:..||.:||.|..:||:..:    |.::.:|.||.||||.
Mouse   988 VAYGF----KAFFSNNWYKLDFMVVIVFCLSLIGKTREDLNPLTSIKFLRALRVLS--------- 1039

  Fly  1611 GLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNIMLVTYLLQFM 1675
                                      ||       :..|.|::.::.......::.||..::..:
Mouse  1040 --------------------------QF-------ERMKVVLRALIKTTLPTVSVFLVCLMIWLL 1071

  Fly  1676 FAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLRE-REWSNNRFHFDDVAKGML 1739
            |:|||||||.|||::|.|   .|:.|.:..:.|.:.....|....| ..|.|.:.:||:|..|.|
Mouse  1072 FSVIGVQLFAGKFYECID---PTKGERFPVFEVMNKSQCEKLLFNESMPWENAKLNFDNVGNGFL 1133

  Fly  1740 TLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQN 1804
            :|..|:||.||..::..:|||...|..|...:...:.:|:||::|...|..:.:.:|.:|..|..
Mouse  1134 SLLQVATFNGWISIMNSAIDSVGVNMQPSFEYNLYMYSYFIIFVIFGLFLPLCMLIGVIIRNFNK 1198

  Fly  1805 EG-EQEYKNCDL---DKNQRNCIEFALKA---KPVRRYIPKHGIQYKVWWFVTSSSFEYTIFILI 1862
            :. :|...|..:   .|.|...::..|.|   ||..|  |::..|..::..||...|...|.:||
Mouse  1199 QKIKQGGSNIFITVKQKKQYRALKKLLYADVQKPTPR--PRNKFQGFLFDLVTHRVFNVIIILLI 1261

  Fly  1863 MINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGS 1927
            .....|:.::...|.......:..:|.||..:|.||.:.||.|||. :||..||||.||::|:.|
Mouse  1262 CFQATTIMIQKDEQSPQMETAIFWMNSIFVMLFTLECILKLTAFRC-HYFTSAWNVHDFMVVIFS 1325

  Fly  1928 FIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEG---IR 1989
            ...::                       ...:.|...:..:..:|..:.|::.:|..|:|   ..
Mouse  1326 ITGLL-----------------------LPLTIGQYFVPPSLVQLILLSRVIHILRPGKGPKVFH 1367

  Fly  1990 TLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRS 2054
            .|:...|.:..||..::|||.|:.||||:.||..|..:..:.|  |...:||:||..::|.||:.
Mouse  1368 DLMLPLILALPALLNISLLIFLVMFIYAIFGMYNFAYVKKEAG--INDVSNFETFGSSMLCLFQV 1430

  Fly  2055 ATGEAWQEIMMSCSAQPDVKCDMNSDTPGE----PCGS-SIAYPYFISFYVLCSFLIINLFVAVI 2114
            .|...|..::.:........||.:...||.    .||| |:...||:|:.::...:|:|:::.:|
Mouse  1431 TTFSGWDGMLDAIFNSQWSDCDPDKINPGTQVKGDCGSPSVGISYFVSYILISWLIIVNMYIVLI 1495

  Fly  2115 MDNFDYLTRDWS-ILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTL---LRKISPPLGFGKLCPH 2175
            |:.....::..| .|.......|.|:|:.:|||   |.:::|...|   ...:.|||...|  |:
Mouse  1496 MEFLSIPSQKKSRTLSEDDFRRFFRVWNRFDPD---RTQYIDSSKLSDFAAALDPPLFMAK--PN 1555

  Fly  2176 RMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNP-KL 2239
            :   .:||:|::|:.:...:.....|.|..:..:.  .|..::...||    |:..:...|| |:
Mouse  1556 K---GQLVAMDLPMAAGDRIHCLDILLAFTKRVMG--KDERVEKILSE----IESGFMLANPFKI 1611

  Fly  2240 LDQVVPPPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEGHP--DSNTVTLQAGLRTLHEVS 2302
            ..:.:.......:..|.   || :||..::.::.|:..:.....|  |.....|.:
Mouse  1612 TYEPITTTLKRKQEAVS---AT-IIQRAYKSYRLRQNDKNVSDTPAIDDRRDDLTS--------- 1663

  Fly  2303 PALKRAISGNLDE 2315
               |.|.||.::|
Mouse  1664 ---KGAHSGKIEE 1673

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	85/306 (28%)
		Ion_trans	1137..1379	CDD:459842	73/251 (29%)
		Ion_trans	1496..1809	CDD:459842	84/319 (26%)
		Ion_trans	1851..2126	CDD:459842	74/282 (26%)
		GPHH	2135..2188	CDD:465306	17/55 (31%)
		Ca_chan_IQ	2198..2273	CDD:462591	14/75 (19%)
Scn7a	NP_033161.2	I. /evidence=ECO:0000250|UniProtKB:Q01118	101..402		90/319 (28%)
		Ion_trans	118..405	CDD:459842	85/305 (28%)
		II. /evidence=ECO:0000250|UniProtKB:Q01118	488..757		83/297 (28%)
		Ion_trans	505..739	CDD:459842	73/252 (29%)
		Na_trans_assoc	743..927	CDD:461936	26/184 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	806..875		13/68 (19%)
		III. /evidence=ECO:0000250|UniProtKB:Q01118	916..1224		93/381 (24%)
		Ion_trans	933..1204	CDD:459842	86/332 (26%)
		Na_channel_gate	1195..1246	CDD:240441	12/52 (23%)
		IV. /evidence=ECO:0000250|UniProtKB:Q01118	1233..1531		88/328 (27%)
		Ion_trans	1250..1497	CDD:459842	73/272 (27%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1647..1681		8/39 (21%)