Protein Alignment Ca-alpha1D and Scn1a

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: NP_001300926.1
• Gene: Scn1a / 20265
• MGIID: 98246
• Length: 2009
• Species: Mus musculus
• Alignment Length: 2022
• Identity: 491/2022 - (24%)
• Similarity: 841/2022 - (41%)
• Gaps: 572/2022 - (28%)

  Fly   747 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFL 811
            ||:.|:..||||.:.|:|:....|..||:.||..||:.:.:..| |....|        |||.|.
Mouse   107 ALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP-PDWTKN--------VEYTFT 162

  Fly   812 VIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLR 876
            .|:|.|.::||:|.||.|.:..:||:.||.||||::....::..:     |..:|.|||.|||||
Mouse   163 GIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFV-----DLGNVSALRTFRVLR 222

  Fly   877 PLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC---------- 931
            .|:.:|.:|.|:.::.::::::..|..:.:|.:|.:.::|:|||:||.|.|...|
Mouse   223 ALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASL 287

  Fly   932 -----RDEITGEYE--------------------------ENIRP---CG----VGYQCPPGYKC 958
                 ...||.:|.                          |.:..   ||    .| |||.||.|
Mouse   288 EEHSIEKNITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAG-QCPEGYMC 351

  Fly   959 YGGWDGPNDGITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVM 1023
            ......||.|.|:||.|..|.|::|:.:|.:.|.::......|.|..:. ::|:.::.||:|:::
Mouse   352 VKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYM-IFFVLVIFLGSFYLI 415

  Fly  1024 NLILGVLSGEFSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQA-------EDIEPDAVGGL 1081
            ||||.|::..: :|:|:|......||..|.||:.|.|:...:...||       ...||.|.|.|
Mouse   416 NLILAVVAMAY-EEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAATTASEHSREPSAAGRL 479

  Fly  1082 I-----------------------------SDGKGKQPNEMDSTEN------------------- 1098
            .                             |.|:.|..:|...:|:
Mouse   480 SDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLT 544

  Fly  1099 ----------------------------------------------------------------- 1098
                                                                             
Mouse   545 YEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLF 609

  Fly  1099 ------------------------------------------------------LGEEMPEV--- 1106
                                                                  :|:.:|||
Mouse   610 VPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIID 674

  Fly  1107 --------QMTESRWRKMKK-------DF-DRVNRRMRRACRKAVKSQAF--------------- 1140
                    ..||:..||.:.       || :..::|.|.....::.:...
Mouse   675 KPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWY 739

  Fly  1141 -------------YWL------------------IIVLVFLNTGVLATEHYGQLDWLDNFQEYTN 1174
                         |||                  |.:.:.|||..:|.|||...:..::.....|
Mouse   740 KFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGN 804

  Fly  1175 VFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLL 1239
            :.|.|:||.||.||:.::....||...:|.||.|:|..|:.|..|.|..     |:||||..|||
Mouse   805 LVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVE-----GLSVLRSFRLL 864

  Fly  1240 RVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGG-------KFNFDGKE 1297
            ||||:.|.|.:|:.|:..:.||:.::.:|.|:|.:.:.|||::|||:||.       |...|.|.
Mouse   865 RVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKL 929

  Fly  1298 EKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNV 1362
            .::.||  .|:.:.|.||:::.|| |...|:..:...|     .|:....|:::.:.||.::||:
Mouse   930 PRWHMN--DFFHSFLIVFRVLCGE-WIETMWDCMEVAG-----QAMCLTVFMMVMVIGNLVVLNL 986

  Fly  1363 FLAI-----AVDNLADADSLSE------------------------------VEKEE-----EPH 1387
            |||:     :.||||..|..:|                              |:|::     :|.
Mouse   987 FLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVKKQKILDEIKPL 1051

  Fly  1388 DESAQKK-----SHSP--------TPTIDGMDDHLSIDIDMEQQELDDEDKM------------- 1426
            |:...:|     :|:.        ...::|....:.....:|:..:|:.|.|
Mouse  1052 DDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVP 1116

  Fly  1427 ------DHETLSDEEV---REMCEEEEEVDE-----EGMITARPRRMSEVNTATKILPI--PPGT 1475
                  |.|.|:.|:.   .::.|.:|:::|     ||...       ::....:..|:  |..|
Mouse  1117 IAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTV-------DIGAPAEEQPVIEPEET 1174

  Fly  1476 ----SFFLFSQTNRF------------------RVFCHWLCNHSNFGNIILCCIMFSSAMLAAEN 1518
                :.|......||                  |..|..:..|:.|...|:..|:.||..||.|:
Mouse  1175 LEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFED 1239

  Fly  1519 -PLRANDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLH-DGAFCRSAFNLLDLLVVCVSLISL 1581
             .:.....:..:|...|..||.:|.:|::||.::||:..: ..|:|     .||.|:|.|||:||
Mouse  1240 IYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWC-----WLDFLIVDVSLVSL 1299

  Fly  1582 VSSS---NAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVI 1643
            .:::   :.:..:|.||.||.||||||::|.:|::.||..::.|:.:|.|::|| ||:.::
Mouse  1300 TANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLV-CLIFWL---- 1359

  Fly  1644 GVQLFKYVVKCVVVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLV 1708
                                           :|:::||.||.|||:.|.:   .|..:.:....|
Mouse  1360 -------------------------------IFSIMGVNLFAGKFYHCVN---TTTGDIFEISEV 1390

  Fly  1709 YDDGDVHKPRLRER----EWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIH 1769
            .:..|..|  |.||    .|.|.:.:||:|..|.|:|..|:||:||..::|.::||......|.:
Mouse  1391 NNHSDCLK--LIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKY 1453

  Fly  1770 NFRPIVAAYYIIYIIIIAFFMVNIFVGFVIVTFQNE----GEQEYKNCDLDKNQRNCIEFALKAK 1830
            .....:..|::|:||..:||.:|:|:|.:|..|..:    |.|:....:..|...|.:: .|.:|
Mouse  1454 EESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMK-KLGSK 1517

  Fly  1831 PVRRYIPKHGIQYK--VWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTA 1893
            ..::.||:.|.:::  |:.|||...|:.:|.|||.:|.||:.::..:|..:.|.:|..:|::|..
Mouse  1518 KPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIV 1582

  Fly  1894 VFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKK 1958
            :|..|.|.||.:.| ..||...||:|||::|:.|.:.:..:|:..|
Mouse  1583 LFTGECVLKLISLR-HYYFTIGWNIFDFVVVILSIVGMFLAELIEK------------------- 1627

  Fly  1959 SAGSNLISINFF---RLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVG 2020
                ..:|...|   ||.|:.|:::|:...:||||||:..:.|..||..:.||:.|:.||||:.|
Mouse  1628 ----YFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFG 1688

  Fly  2021 MQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSATGEAWQEIMMSC--SAQPDVKCDMNSDTPG 2083
            |..|..:..:.|  |....||:||..:::.||:..|...|..::...  |..||  ||.|...||
Mouse  1689 MSNFAYVKREVG--IDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPD--CDPNKVNPG 1749

  Fly  2084 E----PCGS-SIAYPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWS-ILGPHHLDEFIRLWSE 2142
            .    .||: |:...:|:|:.::...:::|:::|||::||...|.:.: .|.....:.|..:|.:
Mouse  1750 SSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEK 1814

  Fly  2143 YDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRT 2207
            :||||...::...:......:.|||...:  |:::   :|::|::|:.|...:.....|||..:.
Mouse  1815 FDPDATQFMEFEKLSQFAAALEPPLNLPQ--PNKL---QLIAMDLPMVSGDRIHCLDILFAFTKR 1874

  Fly  2208 SLSIKTDGNIDDANSELRATIKQIWKRTNP-KLLDQVVPP--PGNDDEVTVGKFYATYLIQDYFR 2269
            .|.  ..|.:|    .||..:::.:..:|| |:..|.:..  ....:||:     |..:.:.|.|
Mouse  1875 VLG--ESGEMD----ALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVS-----AVIIQRAYRR 1928

  Fly  2270 RFKKRKEQEGKEGHPDSNTVTLQAGLRTLHEVSPALKRAISGNLDE--------------LDQEP 2320
            ...||..::....:   |...|:.|...|.:....:.|....::.|              .|:..
Mouse  1929 HLLKRTVKQASFTY---NKNKLKGGANLLVKEDMLIDRINENSITEKTDLTMSTAACPPSYDRVT 1990

  Fly  2321 EPMHRRH 2327
            :|:..:|
Mouse  1991 KPIVEKH 1997

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	95/322 (30%)
		Ion_trans	1137..1379	CDD:459842	88/299 (29%)
		Ion_trans	1496..1809	CDD:459842	97/325 (30%)
		Ion_trans	1851..2126	CDD:459842	86/284 (30%)
		GPHH	2135..2188	CDD:465306	12/52 (23%)
		Ca_chan_IQ	2198..2273	CDD:462591	17/77 (22%)
Scn1a	NP_001300926.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	28..60
		I. /evidence=ECO:0000250|UniProtKB:P04774	110..454		110/360 (31%)
		Ion_trans	127..433	CDD:459842	95/322 (30%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	455..528		12/72 (17%)
		Na_trans_cytopl	555..718	CDD:463401	12/162 (7%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	584..627		0/42 (0%)
		II. /evidence=ECO:0000250|UniProtKB:P04774	750..1022		89/284 (31%)
		Ion_trans	767..980	CDD:459842	73/225 (32%)
		Na_trans_assoc	1006..1213	CDD:461936	29/213 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1129..1163		6/40 (15%)
		III. /evidence=ECO:0000250|UniProtKB:P04774	1200..1514		102/360 (28%)
		Ion_trans	1217..1492	CDD:459842	96/320 (30%)
		Na_channel_gate	1484..1536	CDD:240441	11/52 (21%)
		IV. /evidence=ECO:0000250|UniProtKB:P04774	1523..1821		100/325 (31%)
		Ion_trans	1540..1796	CDD:459842	86/283 (30%)
		S1-S2 loop of repeat IV. /evidence=ECO:0000269|PubMed:27281198	1561..1571		1/9 (11%)
		S3b-S4 loop of repeat IV. /evidence=ECO:0000269|PubMed:27281198	1619..1636		3/39 (8%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1984..2009		3/14 (21%)