DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1D and egl-19

DIOPT Version :9

Sequence 1:	NP_723952.2	Gene:	Ca-alpha1D / 34950	FlyBaseID:	FBgn0001991	Length:	2705	Species:	Drosophila melanogaster
Sequence 2:	NP_001023079.1	Gene:	egl-19 / 177513	WormBaseID:	WBGene00001187	Length:	1877	Species:	Caenorhabditis elegans

Alignment Length:	1964	Identity:	1108/1964 - (56%)
Similarity:	1345/1964 - (68%)	Gaps:	221/1964 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly   720 KGGAAAGAAGQKRQQRRGKPQP-------DRPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLT 777
            :...||.::....::|..:.:|       :|.:|:|.|||:.||:|.|||.||||||||||||..
Worm    33 QAAVAASSSQDATKKRPAQRKPLRQTNVVERSERSLLCLSLNNPIRKLCISIVEWKPFEFLILFM 97

  Fly   778 IFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLL 842
            |.|||||||:|.|||..||:..|..||.:||||:|:||.|||:||:|.||:.|..|||||.||:|
Worm    98 ICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNIL 162

  Fly   843 DFTIVVIGAISTALSQLMKDAFDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALL 907
            ||.|||||.:||.||::....|||||||||||||||||||||||||||||:||:||:||.|||||
Worm   163 DFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALL 227

  Fly   908 VLFVIIIYAIIGLELFSGKLHKACRDEITGEY-EENIRPCGV-----GYQCPPG---------YK 957
            |||||:||||||||||.||||..|.|..||:. :::..|||.     .::|.|.         ::
Worm   228 VLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWE 292

  Fly   958 CYGG--WDGPNDGITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAF 1020
            |...  |.|||:|||||||||||||||||||:|||||||:|.:.||:|.:|.|:||:::||||:|
Worm   293 CSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSF 357

  Fly  1021 FVMNLILGVLSGEFSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDG 1085
            ||:||:||||||||||||.||:.||.|||.|||||:||||:||||||||||||||        ..
Worm   358 FVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEP--------VN 414

  Fly  1086 KGKQPNEMDSTENLGEEMPE------VQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLI 1144
            :.:|.:|..:...:|||..|      ..:..|:|....|..:::|||.|||||:.||||.||||:
Worm   415 EDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLV 479

  Fly  1145 IVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFV 1209
            |:||.|||.||.:|||||.:|||:||...|:||:.||:.|||||||||||..|..|.||||||||
Worm   480 ILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFV 544

  Fly  1210 VIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFL 1274
            ||.||.|.:|....:|.||||||||..||||:|||||||.||.|||:|||||::||.||||||||
Worm   545 VISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFL 609

  Fly  1275 FIVIFALLGMQVFGGKFNFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSS 1339
            |||||||||||||||||||:.::.|.|.|||.|.|||||||||:||||||.|||.||.::|||.:
Worm   610 FIVIFALLGMQVFGGKFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIESFGGVGT 674

  Fly  1340 YGALACIYFIILFICGNYILLNVFLAIAVDNLADADSLSEVEKEEEPHDESAQKKSHSPTPTIDG 1404
            .|.:.|||:|:|||||||||||||||||||||||||||:..||||
Worm   675 LGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEE-------------------- 719

  Fly  1405 MDDHLSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKIL 1469
                       ||||::.||:...|  .::|..|...:|.|.||| |.:|||||||||..|:.:.
Worm   720 -----------EQQEIEGEDEEFEE--GEDEGEEHGMDEPEGDEE-MTSARPRRMSEVPAASTVK 770

  Fly  1470 PIPPGTSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFD 1534
            |||..:|.|:.|.||.|||||:.:.|||.|.|.:|.||:.||||||||:||:||...|.:||.||
Worm   771 PIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFD 835

  Fly  1535 YFFTAVFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLRV 1599
            ||||:|||:|:.||:|.:|.|.|.|:|||:||||||:|||.|||.|.|..::|:|||||||||||
Worm   836 YFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRV 900

  Fly  1600 LRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGN 1664
            ||||||||||||||||||||||||                                    |||||
Worm   901 LRPLRAINRAKGLKHVVQCVIVAV------------------------------------KTIGN 929

  Fly  1665 IMLVTYLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRF 1729
            |||||::||||||:||||||||.||.|.|.||||:.||.|.|:.|:|||..||..::|.||||.|
Worm   930 IMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDF 994

  Fly  1730 HFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIF 1794
            :||:|...|::||.||||||||.||||:||||:|:.|||||.|..||.::|.:||:|||||:|||
Worm   995 NFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIF 1059

  Fly  1795 VGFVIVTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIF 1859
            |||||||||||||:||:||:||||||.||||||||||.|||||::.:||:|||||||.:|||.||
Worm  1060 VGFVIVTFQNEGEREYENCELDKNQRKCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIF 1124

  Fly  1860 ILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIV 1924
            ::|::|||:||.|.|.....:.:.||..|:|||.|||.|.|.|:.|...|||..|.|||||.::|
Worm  1125 LIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVV 1189

  Fly  1925 LGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIR 1989
            :||||||.|.::                     ...|:|||||||||||||||||||||:|||||
Worm  1190 VGSFIDITYGKL---------------------NPGGTNLISINFFRLFRVMRLVKLLSRGEGIR 1233

  Fly  1990 TLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRS 2054
            ||||||:||||||||||||||||||||||:|||.|||:|||...:|..||||.:|..|:||||||
Worm  1234 TLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRS 1298

  Fly  2055 ATGEAWQEIMMSCSAQPDVKCDMNSD------TPGEPCGSSIAYPYFISFYVLCSFLIINLFVAV 2113
            |||||||:||:|||.:.||:||..||      .....||::.||||||||::|||||:|||||||
Worm  1299 ATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNNFAYPYFISFFMLCSFLVINLFVAV 1363

  Fly  2114 IMDNFDYLTRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMA 2178
            ||||||||||||||||||||:||:|||||||||||||||||||||||||||||||||||||||:|
Worm  1364 IMDNFDYLTRDWSILGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLA 1428

  Fly  2179 CKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQV 2243
            |||||||||||||||||.|||||||:|||:|.|.|:||||:||.:||:.||:|||||:..|||:|
Worm  1429 CKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGNIDEANEQLRSAIKRIWKRTHKDLLDEV 1493

  Fly  2244 VPPPGNDDEVTVGKFYATYLIQDYFRRFKKRKEQEGKEGHP--DSNTVTLQAGLRTLHEVSPALK 2306
            |||.|.:|:|||||||||:||||||||||||||.|.|...|  ....:.||||||||||:.|.||
Worm  1494 VPPAGKEDDVTVGKFYATFLIQDYFRRFKKRKEMEAKGVLPAQTPQAMALQAGLRTLHEIGPELK 1558

  Fly  2307 RAISGNLDELD---QEPEPMHRRHHTLFGSVWSSIRRHGNGT---------------FR-RSAKA 2352
            ||||||| |.|   .||||.|||.|:||.::...:...|:.:               |: ||...
Worm  1559 RAISGNL-ETDFNFDEPEPQHRRPHSLFNNLVHRLSGAGSKSPTEHERIEKGSTLLPFQPRSFSP 1622

  Fly  2353 T-----------ASQSNGALAIGGSASAALGVGGSSLVLGSSDPAGGDYLYDTLN-----RSVAD 2401
            |           .||.:....|..|.:.. .|.||:..|.:..|. .::::|..:     |...|
Worm  1623 THSLAGAEGSPVPSQMHRGAPINQSINLP-PVNGSARRLPALPPY-ANHIHDETDDGPRYRDTGD 1685

  Fly  2402 --GVNNITRNIMQARLAAAGKLQDELQGAGSGGELRTFGESISMR-PLAKNGGGAATVAGTLPPE 2463
              |.:..:|.::..|.......::|......|...|:...:..:| |:....|.|..:|| :..|
Worm  1686 RAGYDQSSRMVVANRNLPVDPDEEEQWMRSGGPSNRSDRRNPHLREPMLVARGAALALAG-MSSE 1749

  Fly  2464 ANAINYDNRNRGILLHPYNNVYAPNGALPGHERMIQSTPASPYDQRRLPTSSDMNGLAESLIGGV 2528
            |                |...|.|.|.        ..:...|:..|.:...::.:...:.|||..
Worm  1750 A----------------YEGTYRPVGE--------GKSVRLPFSSRPVLRPAEDSRPVDRLIGQS 1790

  Fly  2529 LAAEGLGKYCDSEFVGTAAREMREALDMTPEEMNLAA------------HQILS-NEHSLS--LI 2578
            |   |||:|.|:..||.|.||:.||..:..:|::|||            |.|.. ||:|.|  |.
Worm  1791 L---GLGRYADARIVGAARREIEEAYSLGEQEIDLAADSLAPLMQHVGMHDIRDINENSRSALLR 1852

  Fly  2579 GSSNGSIFGGSAGG 2592
            .:.|.|....|.||
Worm  1853 PAENSSRQHDSRGG 1866

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:395416	190/291 (65%)
		Ion_trans	1137..1379	CDD:395416	176/241 (73%)
		Ion_trans	1496..1809	CDD:395416	195/312 (63%)
		Ion_trans	1851..2126	CDD:395416	172/280 (61%)
		GPHH	2135..2188	CDD:407139	50/52 (96%)
		Ca_chan_IQ	2199..2271	CDD:400901	51/71 (72%)
egl-19	NP_001023079.1	Ion_trans	105..379	CDD:278921	175/273 (64%)
		Ion_trans	493..714	CDD:278921	162/220 (74%)
		Ion_trans	815..1074	CDD:278921	184/294 (63%)
		Ion_trans	1134..1376	CDD:278921	162/262 (62%)
		GPHH	1384..1447	CDD:293510	59/62 (95%)
		Ca_chan_IQ	1449..1521	CDD:285917	51/71 (72%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160159151
Domainoid	1	1.000	391	1.000	Domainoid score	I402
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H578
Inparanoid	1	1.050	1996	1.000	Inparanoid score	I18
Isobase	1	0.950	-	0	Normalized mean entropy	S2960
OMA	1	1.010	-	-		QHG48156
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	1	1.100	-	-	LDO	PTHR45628
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	1	1.000	-	-		X533
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	15	14.890