DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ca-alpha1D and Cacna1s

DIOPT Version :9

Sequence 1:	NP_723952.2	Gene:	Ca-alpha1D / 34950	FlyBaseID:	FBgn0001991	Length:	2705	Species:	Drosophila melanogaster
Sequence 2:	NP_001074492.1	Gene:	Cacna1s / 12292	MGIID:	88294	Length:	1852	Species:	Mus musculus

Alignment Length:	1930	Identity:	1023/1930 - (53%)
Similarity:	1291/1930 - (66%)	Gaps:	189/1930 - (9%)

- Green bases have known domain annotations that are detailed below.

  Fly   732 RQQRRGKPQPD---RPQRALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPG 793
            |:::..||.|:   ||.||||||:::||||..||.||||||||.:|||||||||:|||||.|.|.
Mouse    12 RKKQPKKPVPEILPRPPRALFCLTLQNPLRKACISIVEWKPFETIILLTIFANCVALAVYLPMPE 76

  Fly   794 SDSNVTNQTLEKVEYVFLVIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQ 858
            .|:|..|..|||:||.||::|:.|..|||:||||:.|..||||:|||:|||.||.:|..:..|.|
Mouse    77 DDNNTLNLGLEKLEYFFLIVFSIEAAMKIIAYGFLFHQDAYLRSGWNVLDFIIVFLGVFTVILEQ 141

  Fly   859 L-----------MKDA-FDVKALRAFRVLRPLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFV 911
            :           .|.| .||||||||||||||||||||||||||||||.|||:|||||||||||:
Mouse   142 VNIIQTNTAPMSSKGAGLDVKALRAFRVLRPLRLVSGVPSLQVVLNSIFKAMLPLFHIALLVLFM 206

  Fly   912 IIIYAIIGLELFSGKLHKAC----RDEITGEYEENIRPC---GVGYQCP-PGYKCYGGWDGPNDG 968
            :|||||||||||.||:||.|    .|.:.....|...||   |.|..|. .|.:|.|||.|||.|
Mouse   207 VIIYAIIGLELFKGKMHKTCYFIGTDIVATVENEKPSPCARTGSGRPCTINGSECRGGWPGPNHG 271

  Fly   969 ITNFDNFGLAMLTVFQCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGE 1033
            ||:|||||.:||||:||:::||||||||.:.||:|::|.|:||:::::||:||::||:|||||||
Mouse   272 ITHFDNFGFSMLTVYQCISMEGWTDVLYWVNDAIGNEWPWIYFVTLILLGSFFILNLVLGVLSGE 336

  Fly  1034 FSKERNKAKNRGDFQKLREKQQIEEDLRGYLDWITQAE--DIEPDAVGGLISDGKGKQPNEMDST 1096
            |:|||.|||:||.|||||||||:|||||||:.||||.|  |::....|.|..|..|.....:...
Mouse   337 FTKEREKAKSRGTFQKLREKQQLEEDLRGYMSWITQGEVMDVDDLREGKLSLDEGGSDTESLYEI 401

  Fly  1097 ENLGEEMPEVQMTESRWRKMKKDFDRVNRRMRRACRKAVKSQAFYWLIIVLVFLNTGVLATEHYG 1161
            |.|.:.:..::    .||:.       ||..|..|...|||:.||||:|::|.|||..:|:||:.
Mouse   402 EGLNKIIQFIR----HWRQW-------NRVFRWKCHDLVKSKVFYWLVILIVALNTLSIASEHHN 455

  Fly  1162 QLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLGFQGYFVSLFNRFDCFVVIGSITETLLTNTGMMP 1226
            |..||.:.|:..|...:.|||.|||:|||.||.:.||:|:|||||||||...|.|.||..:|.|.
Mouse   456 QPLWLTHLQDVANRVLLTLFTIEMLMKMYGLGLRQYFMSIFNRFDCFVVCSGILEILLVESGAMS 520

  Fly  1227 PLGVSVLRCVRLLRVFKVTKYWRSLSNLVASLLNSIQSIASLLLLLFLFIVIFALLGMQVFGGKF 1291
            |||:|||||:||||:||:||||.|||||||||||||:|||||||||||||:||||||||:|||::
Mouse   521 PLGISVLRCIRLLRLFKITKYWTSLSNLVASLLNSIRSIASLLLLLFLFIIIFALLGMQLFGGRY 585

  Fly  1292 NFDGKEEKYRMNFDCFWQALLTVFQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGN 1356
            :|:..|.: |.|||.|.|||::|||::||||||:|||.||.||||.:..|.|.||||||||:|||
Mouse   586 DFEDTEVR-RSNFDNFPQALISVFQVLTGEDWNSVMYNGIMAYGGPTYPGVLVCIYFIILFVCGN 649

  Fly  1357 YILLNVFLAIAVDNLADADSLSEVEKEEEPHDESAQKKSHSPTPTIDGMDDHLSIDIDMEQQ--- 1418
            ||||||||||||||||:|:||:..:|.:.  :|..::|.....|. ...::..::...:||:
Mouse   650 YILLNVFLAIAVDNLAEAESLTSAQKAKA--EERKRRKMSKGLPD-KSEEERATVTKKLEQKSKG 711

  Fly  1419 ---------ELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVNTATKILPIPPG 1474
                     ::|:.:...:|........:...::||.:.|..::.|||.::|:....|.:|||..
Mouse   712 EGIPTTAKLKIDEFESNVNEVKDPYPSADFPGDDEEDEPEIPVSPRPRPLAELQLKEKAVPIPEA 776

  Fly  1475 TSFFLFSQTNRFRVFCHWLCNHSNFGNIILCCIMFSSAMLAAENPLRANDDLNKVLNKFDYFFTA 1539
            :|||:||.||:.||.||.:.|.:.|.|.||..|:.|||.||||:|:||:...|::|..|||.|||
Mouse   777 SSFFIFSPTNKIRVLCHRIVNATWFTNFILLFILLSSAALAAEDPIRADSMRNQILEYFDYVFTA 841

  Fly  1540 VFTIELILKLISYGFVLHDGAFCRSAFNLLDLLVVCVSLISLVSSSNAISVVKILRVLRVLRPLR 1604
            |||:|::||:.:||..||.|:|||:.||:||||||.|||||:...|:||||||||||||||||||
Mouse   842 VFTVEIVLKMTTYGAFLHKGSFCRNYFNILDLLVVAVSLISMGLESSAISVVKILRVLRVLRPLR 906

  Fly  1605 AINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLFKYVVKCVVVAIKTIGNIMLVT 1669
            |||||||||||||||.||::|||||||||.|||||||            |
Mouse   907 AINRAKGLKHVVQCVFVAIRTIGNIVLVTTLLQFMFA------------C--------------- 944

  Fly  1670 YLLQFMFAVIGVQLFKGKFFKCTDGSKMTQDECYGTYLVYDDGDVHKPRLREREWSNNRFHFDDV 1734
                     ||||||||||:.|.|.||||::||.|.|.:|.|||..:..||.|:|.:|.||||:|
Mouse   945 ---------IGVQLFKGKFYSCNDLSKMTEEECRGYYYIYKDGDPTQIELRPRQWIHNDFHFDNV 1000

  Fly  1735 AKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIVAAYYIIYIIIIAFFMVNIFVGFVI 1799
            ...|::|||||||||||.|||.:||||:|:.||::|.|..:|.::|||||:|||||:||||||||
Mouse  1001 LSAMMSLFTVSTFEGWPQLLYKAIDSNEEDTGPVYNNRVEMAIFFIIYIILIAFFMMNIFVGFVI 1065

  Fly  1800 VTFQNEGEQEYKNCDLDKNQRNCIEFALKAKPVRRYIPKHGIQYKVWWFVTSSSFEYTIFILIMI 1864
            ||||.:||.|||||:||||||.|:::||||:|:|.||||:..||:||:.||||.|||.:|.|||:
Mouse  1066 VTFQEQGETEYKNCELDKNQRQCVQYALKARPLRCYIPKNPYQYQVWYVVTSSYFEYLMFALIML 1130

  Fly  1865 NTVTLAMKFYNQPLWYTELLDALNMIFTAVFALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFI 1929
            ||:.|.|:.|||......:.|.||:.||.:|.||.|.||.||:.:.||||.||||||:||:||.|
Mouse  1131 NTICLGMQHYNQSEQMNHISDILNVAFTIIFTLEMVLKLIAFKPRGYFGDPWNVFDFLIVIGSII 1195

  Fly  1930 DIVYSEIKSKDTSQIAECDIVEGCKSTKKSAGSNLISINFFRLFRVMRLVKLLSKGEGIRTLLWT 1994
            |::.|||.:...|......:..||.:......:. ||..||||||||||||||::.||:||||||
Mouse  1196 DVILSEIDTFLASSGGLYCLGGGCGNVDPDESAR-ISSAFFRLFRVMRLVKLLNRAEGVRTLLWT 1259

  Fly  1995 FIKSFQALPYVALLIVLLFFIYAVVGMQVFGKIALDGGNAITANNNFQTFQQAVLVLFRSATGEA 2059
            ||||||||||||||||:|||||||:|||:|||||:..|..|..|||||||.||||:|||.|||||
Mouse  1260 FIKSFQALPYVALLIVMLFFIYAVIGMQMFGKIAMVDGTQINRNNNFQTFPQAVLLLFRCATGEA 1324

  Fly  2060 WQEIMMSCSAQPDVKCDMNSD-TPGE--PCGSSIAYPYFISFYVLCSFLIINLFVAVIMDNFDYL 2121
            ||||:::||.  ...||..|| .|||  .||::.||.||||||:||:||||||||||||||||||
Mouse  1325 WQEILLACSY--GKLCDPESDYAPGEEHTCGTNFAYYYFISFYMLCAFLIINLFVAVIMDNFDYL 1387

  Fly  2122 TRDWSILGPHHLDEFIRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRLVSMN 2186
            |||||||||||||||..:|:||||:|||||||||||||||:|.|||||||.||||:||||||.||
Mouse  1388 TRDWSILGPHHLDEFKAIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKFCPHRVACKRLVGMN 1452

  Fly  2187 MPLNSDGTVLFNATLFAVVRTSLSIKTDGNIDDANSELRATIKQIWKRTNPKLLDQVVPPPGNDD 2251
            |||||||||.|||||||:|||:|.|||:||.:.||.||||.||:|||||:.||||||:||.| ||
Mouse  1453 MPLNSDGTVTFNATLFALVRTALKIKTEGNFEQANEELRAIIKKIWKRTSMKLLDQVIPPIG-DD 1516

  Fly  2252 EVTVGKFYATYLIQDYFRRFKKRKEQEGKEGH-PDSNTVTLQAGLRTL-HEVSPALKRAISGNL- 2313
            ||||||||||:|||::||:|.||  ||...|: |..:||.:||||||: .|.:|.:.|||||:|
Mouse  1517 EVTVGKFYATFLIQEHFRKFMKR--QEEYYGYRPKKDTVQIQAGLRTIEEEAAPEIHRAISGDLT 1579

  Fly  2314 --DELDQE------PEPMHRRHHTLFGSVWSSIRRHGN--------------------------- 2343
              :||::.      .|.:.||...|||.|.:.:.|..:
Mouse  1580 AEEELERAMVEAAMEEGIFRRTGGLFGQVDNFLERTNSLPPVMANQRPLQFAEIEMEELESPVFL 1644

  Fly  2344 -------GTFRRSAKATASQSNGALAIGGSASAALGVGGSSLVLGSSDPAGGDYLYDTLNRSVAD 2401
                   || ...|:|..:.:|..:|.|.|:.             .::|......|:......||
Mouse  1645 EDFPQNPGT-HPLARANTNNANANVAYGNSSH-------------RNNPVFSSICYEREFLGEAD 1695

  Fly  2402 GVNNITRNIMQARLAAAGKLQDELQGAGSGGELRTFGESISMRPLAKNGGGAATVAGTLPPEANA 2466
              ..:||.         |.|....:  .||...|:..:.:. ||:.:.|    ...|.:||....
Mouse  1696 --MPVTRE---------GPLSQPCR--ASGPHSRSHVDKLK-RPMTQRG----MPEGQVPPSPCQ 1742

  Fly  2467 INYDNRNRGILLHPYNNVYAPNGALPGHERMIQSTPASPYDQRRLPTSSDMNGLAES---LIGGV 2528
            :......     ||...    .|..| ..|.:: ||.|...:..:|.:|.....|.:   ||...
Mouse  1743 VTGAKAE-----HPVQK----EGKGP-TSRFLE-TPNSRNFEEHVPRNSAHRCTAPATAMLIQEA 1796

  Fly  2529 LAAEGLGKY-CDSEFVGTAAREMREALDMTPEEMNLAAHQILSNE 2572
            |...||... .|:.||....:.:.:|..|.|||:.:||.::|..|
Mouse  1797 LVRGGLDSLAADANFVMATGQALADACQMEPEEVEVAATELLKQE 1841

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:395416	180/294 (61%)
		Ion_trans	1137..1379	CDD:395416	162/241 (67%)
		Ion_trans	1496..1809	CDD:395416	186/312 (60%)
		Ion_trans	1851..2126	CDD:395416	173/277 (62%)
		GPHH	2135..2188	CDD:407139	42/52 (81%)
		Ca_chan_IQ	2199..2271	CDD:400901	53/71 (75%)
Cacna1s	NP_001074492.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..23		4/10 (40%)
		I. /evidence=ECO:0000305	38..337		183/298 (61%)
		Ion_trans	68..345	CDD:278921	165/276 (60%)
		Selectivity filter of repeat I. /evidence=ECO:0000250\|UniProtKB:P07293	290..293		0/2 (0%)
		Binding to the beta subunit. /evidence=ECO:0000269\|PubMed:28351836	357..374		13/16 (81%)
		II. /evidence=ECO:0000305	418..664		162/246 (66%)
		Ion_trans	449..672	CDD:278921	152/223 (68%)
		Selectivity filter of repeat II. /evidence=ECO:0000250\|UniProtKB:P07293	612..615		2/2 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	675..712		6/39 (15%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	731..757		4/25 (16%)
		III. /evidence=ECO:0000305	768..1068		197/335 (59%)
		Ion_trans	816..1075	CDD:278921	178/294 (61%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	988..1077		58/88 (66%)
		Selectivity filter of repeat III. /evidence=ECO:0000250\|UniProtKB:P07293	1012..1015		2/2 (100%)
		IV. /evidence=ECO:0000305	1105..1384		171/281 (61%)
		Ion_trans	1135..1392	CDD:278921	162/259 (63%)
		Selectivity filter of repeat IV. /evidence=ECO:0000250\|UniProtKB:P07293	1321..1324		2/2 (100%)
		Dihydropyridine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1337..1403		51/65 (78%)
		Phenylalkylamine binding. /evidence=ECO:0000250	1349..1392		33/42 (79%)
		Phenylalkylamine binding. /evidence=ECO:0000250\|UniProtKB:P07293	1349..1391		32/41 (78%)
		GPHH	1400..1463	CDD:293510	52/62 (84%)
		Ca_chan_IQ	1465..1536	CDD:285917	53/71 (75%)
		Interaction with calmodulin. /evidence=ECO:0000250\|UniProtKB:Q13698	1522..1542		14/21 (67%)
		CAC1F_C	<1679..1840	CDD:293490	44/189 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	383	1.000	Domainoid score	I823
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2021	1.000	Inparanoid score	I52
Isobase	1	0.950	-	0	Normalized mean entropy	S2960
OMA	1	1.010	-	-		QHG48156
OrthoDB	1	1.010	-	-		D172471at2759
OrthoFinder	1	1.000	-	-		FOG0000179
OrthoInspector	1	1.000	-	-		otm43218
orthoMCL	1	0.900	-	-		OOG6_100303
Panther	1	1.100	-	-	O	PTHR45628
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	1	1.000	-	-		X533
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.960