Protein Alignment Ca-alpha1D and scn2a

DIOPT Version :10

• Sequence 1: NP_723952.2
• Gene: Ca-alpha1D / 34950
• FlyBaseID: FBgn0001991
• Length: 2705
• Species: Drosophila melanogaster
• Sequence 2: XP_031749252.1
• Gene: scn2a / 100486750
• XenbaseID: XB-GENE-6447552
• Length: 1970
• Species: Xenopus tropicalis
• Alignment Length: 1954
• Identity: 469/1954 - (24%)
• Similarity: 808/1954 - (41%)
• Gaps: 586/1954 - (29%)

  Fly   747 ALFCLSVKNPLRALCIRIVEWKPFEFLILLTIFANCIALAVYTPYPGSDSNVTNQTLEKVEYVFL 811
            ||:.|:..||:|...|:|:....|..||:.||..||:.:.:..|         .:..:.|||.|.
 Frog   107 ALYILTPFNPVRQAAIKILVHSLFNMLIMCTILTNCVFMTLSNP---------PEWTKNVEYTFT 162

  Fly   812 VIFTAECVMKILAYGFVLHNGAYLRNGWNLLDFTIVVIGAISTALSQLMKDAFDVKALRAFRVLR 876
            .|:|.|.::||||.||.|....:||:.||.|||:::|:..::..:     :..:|.|||.|||||
 Frog   163 GIYTLESLIKILARGFCLEKFTFLRDPWNWLDFSVIVLAYVTEFV-----NLGNVSALRTFRVLR 222

  Fly   877 PLRLVSGVPSLQVVLNSILKAMVPLFHIALLVLFVIIIYAIIGLELFSGKLHKAC---------- 931
            .|:.:|.:|.|:.::.::::::..|..:.:|.:|.:.::|:|||:||.|.|...|
 Frog   223 ALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRNKCLLWPYNSSVD 287

  Fly   932 -------RDEITGEYEENIRP--CG----VGYQCPPGYKCYGGWDGPNDGITNFDNFGLAMLTVF 983
                   .:.:..:.|....|  ||    .| :||.||.|......||.|.|:||.|..|.|::|
 Frog   288 ISLYEHFNESLFYKLENQKDPLVCGNSSDTG-KCPEGYVCVKAGRNPNYGYTSFDTFNWAFLSLF 351

  Fly   984 QCVTLEGWTDVLYSIQDAMGSDWQWMYFISMVILGAFFVMNLILGVLSGEFSKERNKA------K 1042
            :.:|.:.|.::......|.|..:. ::|:.::.||:|:::||||.|::..: :|:|:|      :
 Frog   352 RLMTQDCWENLYQLTLRAAGKTYM-IFFVLVIFLGSFYLVNLILAVVAMAY-EEQNQATIEEAVQ 414

  Fly  1043 NRGDFQKLREKQQIEEDLRGYLDWITQAEDIEPDAVGGLISDGKGKQPNEMDSTENLG-EEMPEV 1106
            ...:||:|:::|:                  |..|:...::|..|:   ..:.:|.:| |:..|.
 Frog   415 KEAEFQRLKKQQE------------------EAQALAAAVADVFGE---SQELSEEVGLEDASEC 458

  Fly  1107 QMTESR-----------WRKMKK------------------------------DFDRVNRRM--- 1127
            ..|.|:           |||.:|                              :|.|..||:
 Frog   459 SSTVSKLSSKSAKERRNWRKQRKQVAHCDGQEKGDKEKCYKSGSEDSIKRKGLNFTREGRRLEEY 523

  Fly  1128 ----------------------------------------------------------------- 1127
                                                                             
 Frog   524 RKSSMPHQSLLSIQGPVFSPRRNSKTSIFSFKDRAKDVGSENDFADDERSTFEEDSRRDPLFVPG 588

  Fly  1128 -RRACRKAVKSQ----------------------------------------------------- 1138
             .||.|.:..||
 Frog   589 RHRARRNSNISQTSRSSRILPLLSMNGKMHSTVDCNGVVSLVGGSSAPIGLFPEGTTTETDRKRR 653

  Fly  1139 -------------------------------------------AFY--------------WL--- 1143
                                                       .:|              ||
 Frog   654 PSSYKISMDLMEDPSLRQRAQSIASILTNTMEELQESRRKCPSCWYKFANCFLIWDCCEAWLKVK 718

  Fly  1144 ---------------IIVLVFLNTGVLATEHYGQLDWLDNFQEYTNVFFIGLFTCEMLLKMYSLG 1193
                           |.:.:.|||..:|.||.....:..:.....|..|.|:||.||:||:.:|.
 Frog   719 RIVKLIVMDPFVDLAITISIVLNTIFMAVEHAHMTPYFISVLTTGNQVFTGIFTAEMVLKLIALD 783

  Fly  1194 FQGYFVSLFNRFDCFVVIGSITETLLTNTGMMPPLGVSVLRCVRLLRVFKVTKYWRSLSNLVASL 1258
            ...||...:|.||..:|..|:.|..|::||     |.||||..|||||||:.|.|.:|:.|:..:
 Frog   784 PYYYFQEGWNIFDGLIVSLSLMELGLSSTG-----GFSVLRSFRLLRVFKLAKSWPTLNKLIKII 843

  Fly  1259 LNSIQSIASLLLLLFLFIVIFALLGMQVFGGKFNFDGKE---------EKYRMNFDCFWQALLTV 1314
            .||:.::.:|.|:|.:.:.|||::|||:||..:    ||         |..|.:.:.|:.:.|.|
 Frog   844 GNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY----KECVCKIAENCELPRWHMNDFFHSFLIV 904

  Fly  1315 FQIMTGEDWNAVMYVGINAYGGVSSYGALACIYFIILFICGNYILLNVFLAIAV-----DNLADA 1374
            |:::.|| |...|:..:...|     .::..:.|:::.:.||.::||:|||:.:     |||:..
 Frog   905 FRVLCGE-WIETMWDCMEVAG-----QSMCLLVFMLVMVIGNLVVLNLFLALLLSSFSSDNLSAT 963

  Fly  1375 DSLSEVEKEE--------------------------EPHDESAQKKSHSPTPTIDGMDD-----H 1408
            |..||:...:                          :...:..:.|....||   |.:|     :
 Frog   964 DEDSEINNIQIAVGRIQRGVASGKIILREFFEKLFLQKQKDINETKQLEETP---GKNDTCVLGN 1025

  Fly  1409 LSIDIDMEQQELDDEDKMDHETLSDEEVREMCEEEEEVDEEGMITARPRRMSEVN--TATKILPI 1471
            ..::|..|:.::.|.|.|..|..|  .|.:..|.|:|.            |:.:|  :.|..:||
 Frog  1026 AVVEILKEKNDVRDSDGMTSEIGS--SVEKSAENEKEY------------MTVINKTSLTVAVPI 1076

  Fly  1472 PPGTSFFLFSQTNRF-------------------------------------------------- 1486
            .||.|.|....|..|
 Frog  1077 APGESDFENPNTEEFSSESDPEETKEKIKVSSSSEGSTIDIRAPGVERIEEFLDYEYEETFDPEP 1141

  Fly  1487 ----------------------------RVFCHWLCNHSNFGNIILCCIMFSSAMLAAEN-PLRA 1522
                                        |..|:.:..||.|.:.|:..|:.||..||.|: .:..
 Frog  1142 CFTDDCVASFTCCQINIENGTGRDWWNLRKTCYTIVEHSWFESFIIFMILLSSGALAFEDIYVEQ 1206

  Fly  1523 NDDLNKVLNKFDYFFTAVFTIELILKLISYGFVLH-DGAFCRSAFNLLDLLVVCVSLISLVSSS- 1585
            ..::..:|...|..|..:|.:|::||.::|||:.: ..|:|     .||.::|.:||:||::::
 Frog  1207 RRNVKAILEYADKVFAYIFIMEMLLKWVAYGFIKYFTNAWC-----WLDFIIVDISLVSLIANAL 1266

  Fly  1586 --NAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIVLVTCLLQFMFAVIGVQLF 1648
              :.:..:|.||.||.||||||::|.:|::.||..:|.|:.:|.|::|| ||:.::
 Frog  1267 GYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLV-CLIFWL--------- 1321

  Fly  1649 KYVVKCVVVAIKTIGNIMLVTYLLQFMFAVIGVQLFKGKFFKC---TDGSKMTQDECYGTYLVYD 1710
                                      :|:::||.||.|||:.|   |.|......:...    |.
 Frog  1322 --------------------------IFSIMGVNLFAGKFYYCVNTTSGVPFPATDVNN----YS 1356

  Fly  1711 DGDVHKPRLREREWSNNRFHFDDVAKGMLTLFTVSTFEGWPGLLYVSIDSNKENGGPIHNFRPIV 1775
            |..::........|.|.:.:||:|..|.|.|..|:||:||..::|.::||.:.|..|.:.....:
 Frog  1357 DCRMYINTTGAARWKNVKVNFDNVGAGYLALLQVATFKGWMPIMYAAVDSREINEQPKYENNTTM 1421

  Fly  1776 AAYYIIYIIIIAFFMVNIFVGFVIVTFQNE----GEQEYKNCDLDKNQRNCIEFALK-------A 1829
            ..|::.:||..:||.:|:|:|.:|..|..:    |.|:....:..|...|    |:|       .
 Frog  1422 YLYFVAFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYN----AMKKLGSKKPQ 1482

  Fly  1830 KPVRRYIPKHGIQYKVWWFVTSSSFEYTIFILIMINTVTLAMKFYNQPLWYTELLDALNMIFTAV 1894
            ||:.|  |.:..|..::.|||..:|:..|.|||.:|.||:.::..:|.......|..:|.:|..:
 Frog  1483 KPIPR--PANCCQGLIFDFVTKQAFDIIIMILIFLNMVTMMIETDDQSQEMEMYLYRINAVFIIL 1545

  Fly  1895 FALEFVFKLAAFRFKNYFGDAWNVFDFIIVLGSFIDIVYSEIKSKDTSQIAECDIVEGCKSTKKS 1959
            |..||:.||.:.| :.||...||:||.::|:.|.:.:..:             ||:|     :..
 Frog  1546 FTGEFLLKLISLR-QYYFTIGWNIFDLVVVILSIVGMFLA-------------DIIE-----RYF 1591

  Fly  1960 AGSNLISINFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIVLLFFIYAVVGMQVF 2024
            ....|..:  .||.|:.|:::|:...:||||||:..:.|..||..:.||:.|:.||||:.||..|
 Frog  1592 FSPTLFRV--IRLARIGRILRLIKAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNF 1654

  Fly  2025 GKIALDGGNAITANNNFQTFQQAVLVLFRSATGEAWQEIM--MSCSAQPDVKCDMNSDTPGE--- 2084
            ..:....|  |....||:||..:::.||:..|...|..::  :..|.:||  ||.|::.||.
 Frog  1655 AYVKKQSG--IDDMFNFETFGNSMICLFQITTSAGWDLLLEPILNSGEPD--CDPNAEHPGSLFK 1715

  Fly  2085 -PCGS-SIAYPYFISFYVLCSFLIINLFVAVIMDNFDYLTRDWS-ILGPHHLDEFIRLWSEYDPD 2146
             .||: |:...:|:|:.::...:::|:::|||::||...|.:.: .||....:.|..:|.::||.
 Frog  1716 GDCGNPSVGIFFFVSYIIISFLIVVNMYIAVILENFSVATEESAEPLGEDDFEMFYEVWEKFDPS 1780

  Fly  2147 AKGRIKHLDVVTLLRKISPPLGFGKLCPHRMACKRLVSMNMPLNSDGTVLFNATLFAVVRTSLSI 2211
            |...|::..:......:.|||...|  |:.:   :|::|::|:.|...:.....|||..:..|.
 Frog  1781 ASQFIEYSKLSDFADALDPPLRVPK--PNNI---QLIAMDLPMVSGDRIHCLDILFAFTKRVLG- 1839

  Fly  2212 KTDGNIDDANSELRATIKQIWKRTNPKLLDQVVPPPGNDDEVTVGK---FYATYLIQDYFRRFKK 2273
             ..|.:|    .||..:::.:..:||..:      |......|:.:   ..:..:||..:|||:.
 Frog  1840 -EGGEMD----SLRQPMEERFMTSNPSKV------PYEPITTTLRRKQEEQSAVVIQRCYRRFRL 1893

  Fly  2274 RKEQ 2277
            :|.|
 Frog  1894 KKRQ 1897

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ca-alpha1D	NP_723952.2	Ion_trans	767..1042	CDD:459842	91/303 (30%)
		Ion_trans	1137..1379	CDD:459842	87/383 (23%)
		Ion_trans	1496..1809	CDD:459842	94/324 (29%)
		Ion_trans	1851..2126	CDD:459842	84/281 (30%)
		GPHH	2135..2188	CDD:465306	13/52 (25%)
		Ca_chan_IQ	2198..2273	CDD:462591	17/77 (22%)
scn2a	XP_031749252.1	Ion_trans	127..408	CDD:459842	90/297 (30%)
		Na_trans_cytopl	532..678	CDD:463401	5/145 (3%)
		Ion_trans	727..940	CDD:459842	71/227 (31%)
		Na_trans_assoc	966..1175	CDD:461936	31/225 (14%)
		Ion_trans	1179..1454	CDD:459842	93/319 (29%)
		Na_channel_gate	1446..1498	CDD:240441	12/57 (21%)
		Ion_trans	1503..1758	CDD:459842	84/279 (30%)
		GPHH	1770..1817	CDD:465306	13/51 (25%)