DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Cul3 and Cul4b

DIOPT Version :9

Sequence 1:	NP_723909.2	Gene:	Cul3 / 34896	FlyBaseID:	FBgn0261268	Length:	934	Species:	Drosophila melanogaster
Sequence 2:	NP_001103612.1	Gene:	Cul4b / 72584	MGIID:	1919834	Length:	970	Species:	Mus musculus

Alignment Length:	954	Identity:	322/954 - (33%)
Similarity:	500/954 - (52%)	Gaps:	121/954 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 AGSNNFNGATVGNVDSAVPCRIARQNVLGQQARSMN----------------------------- 64
            |.:.....||.||. |..|...|::..|...:.|.|
Mouse    90 AAAQEVRSATDGNT-STTPPTSAKKRKLNSSSSSSNSSNEREDFDSTSSSSTPPQPRDSASPSTS 153

  Fly    65 ---INVPNASS----MQQHQQSQNRLSAQGL-------ASASRRSYHLAASGGRVRADNRINTNH 115
               :.||.|:|    :|:..:.::.|...|:       :|:|..|....|:..:.:...::.|..
Mouse   154 SFCLGVPVATSSHVPIQKKLRFEDTLEFVGIDTKMAEESSSSSSSSSPTAATSQQQQQQQLKTKS 218

  Fly   116 PLTPTPLSMPVPAPAAPAVVKTETSATTSGPSTSASASAESTEKRFKEIARKYPLWLPEYKRRAF 180
            .|    :|.......|..:.|:.|:.::...|...||. :...|.||:
Mouse   219 IL----ISSVASVHHANGLAKSSTAVSSFANSKPGSAK-KLVIKNFKD----------------- 261

  Fly   181 NASMDEKYVETIWASLKNAIQEIQKKNNSGLSFEQLYRNAYNMVLHKHGNRLYYGLREVVSEHLE 245
            ...:.|.|.:..|..||.|::.||...:...:.|:||:...|:..||....||..||::..:|::
Mouse   262 KPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSHKISANLYKQLRQICEDHIK 326

  Fly   246 ---HKVRADVLEALHSNFLPKLNQAWTDHQTSMVMIRDILMYMDRVYVQQRE-VDNVYNLGLILF 306
               |:.|.|.|:::  .||.|:::.|.:|...|:|||.|.:::||.||.|.. :.:::::||.||
Mouse   327 AQIHQFREDSLDSV--LFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELF 389

  Fly   307 RDQVVRYSEIQKALREKLLGMVMEERHGEAINHLAIKNACSMLITLGINSRTVYEEDFEKPFLAQ 371
            |..::...::|....:.:|.::..||:||||:...:::..|||..|     .:|::.||:.||.:
Mouse   390 RAHIISDQKVQTKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDL-----QIYQDSFEQQFLQE 449

  Fly   372 SAAFYKFESQNFLAENNAGVYIKKVEARITEESSRAALYLDKDTEPRIVRVVEEELIKKHMRPIV 436
            :...|..|.|..:.|.....|:..|..|:.||:.|...|||:.|:..::..||::|:.:|:..|:
Mouse   450 TNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIASVEKQLLGEHLTAIL 514

  Fly   437 EMENSGVVYMIKNSKTEDLACTYKLFSRLKEEGLKVIADTMSAYLREQGRMLVKEEENGNTNPIT 501
            :   .|:..::..::.:||:..|:||||:: .|::|:......|::..|..:|...|...    |
Mouse   515 Q---KGLNSLLDENRIQDLSLLYQLFSRVR-GGVQVLLQQWIEYIKAFGSTIVINPEKDK----T 571

  Fly   502 FVQNLLDLKDRFDQFLVHSFANDRIFKNVISSDFEHFLNLN-NKSPEYLSLFIDDKLKKGGKGMS 565
            .||.|||.||:.|..:...|..:..|.|.:...||.|:|.. ||..|.::.::|.||:.|.|..:
Mouse   572 MVQELLDFKDKVDHIIDTCFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEAT 636

  Fly   566 EQEIESILDKTMVLFRFLLEKDVFERYYKTHLAKRLLLNKSVSDDFEKNMISKLKTECGCQFTSK 630
            ::|:|.:|||.|::|||:..|||||.:||..||||||:.||.|.|.||:|:||||.|||..||||
Mouse   637 DEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSK 701

  Fly   631 LEGMFKDMSVSNTIMDEFKNFVNNNNLSLGGVELTVRILTTGFWPTQTATPNCNIPAAPREAFDI 695
            ||||||||.:|..||.:||.::.|.|:. |.:||||.|||.|:|||.... ..::|....:..:|
Mouse   702 LEGMFKDMELSKDIMIQFKQYMQNQNVP-GNIELTVNILTMGYWPTYVPM-EVHLPPEMVKLQEI 764

  Fly   696 FKNFYLNKHSGRQLTLQPQMGTAYINAVFYGRKAVESEKDKDAPSSSSSGCGVPTTTRKHILQVS 760
            ||.|||.|||||:|..|..:|...:.|.|     .|.:|:                     ||||
Mouse   765 FKTFYLGKHSGRKLQWQSTLGHCVLKAEF-----KEGKKE---------------------LQVS 803

  Fly   761 TYQMCVLLLFNNRDVLTYDDIHQETDIPERELVRALQSLSMGKPAQRLLVRNSKTKTKDIEPTDE 825
            .:|..|||:||..:..:.::|...|.|.:.||.|.||||:.||  .|:|.:|  .|.||||..|:
Mouse   804 LFQTMVLLMFNEGEEFSLEEIKHATGIEDGELRRTLQSLACGK--ARVLAKN--PKGKDIEDGDK 864

  Fly   826 FYVNDAFNSKFHRVKIQTVAAKGESEPERKETRGKVDEDRKHEIEAAIVRIMKARKRLAHNLLVS 890
            |..||.|..|..|:||..:..| |:..|:..|..:|.:||:::|:||||||||.||.|:||||||
Mouse   865 FICNDDFKHKLFRIKINQIQMK-ETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVS 928

  Fly   891 DVTSQLKSRFLPSPVFIKKRIEGLIEREYLQRSPEDRKVYNYLA 934
            :|.:|||  |...|..:|||||.||:|:|::|..|:...|||:|
Mouse   929 EVYNQLK--FPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA 970

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Cul3	NP_723909.2	Cullin	196..831	CDD:279260	233/639 (36%)
		CULLIN	582..724	CDD:214545	75/141 (53%)
		Cullin_Nedd8	866..926	CDD:287520	34/59 (58%)
Cul4b	NP_001103612.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..157		11/67 (16%)
		Nuclear localization signal. /evidence=ECO:0000250\|UniProtKB:Q13620	112..115		0/2 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	189..211		4/21 (19%)
		Cullin	275..870	CDD:279260	233/641 (36%)
		CULLIN	653..793	CDD:214545	75/141 (53%)
		Cullin_Nedd8	899..964	CDD:214883	38/66 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5647
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53685
OrthoDB	1	1.010	-	-		D1040292at2759
OrthoFinder	1	1.000	-	-		FOG0000491
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.830