DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ck and MYO5B

DIOPT Version :9

Sequence 1:	NP_001285949.1	Gene:	ck / 34882	FlyBaseID:	FBgn0000317	Length:	2167	Species:	Drosophila melanogaster
Sequence 2:	NP_001073936.1	Gene:	MYO5B / 4645	HGNCID:	7603	Length:	1848	Species:	Homo sapiens

Alignment Length:	1796	Identity:	511/1796 - (28%)
Similarity:	787/1796 - (43%)	Gaps:	376/1796 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    64 GVEDMISLGDLHEAGILRNLLIRYKE-NLIYTYTGSILVAVNPYQILPIYTGDQIKLYKERKIGE 127
            |..|:.:|..|||..:|.||.:|:.| |.||||.|.:|||:|||:.||||..|.|..|..:.:|:
Human    70 GENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYTYSGQNMGD 134

  Fly   128 LPPHIFAIGDNAYAHMKRYRQDQCIVISGESGAGKTESTKLILQYLAAISGKHSW--IEQQILEA 190
            :.|||||:.:.||..|.|..::|.|::|||||||||.|.|..::|.|.:.|..|.  ||:::|.:
Human   135 MDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASETNIEEKVLAS 199

  Fly   191 NPILEAFGNAKTIRNDNSSRFGKYIDIHFSANGVIEGAKIEQYLLEKSRIVSQNHSERNYHVFYC 255
            :||:||.|||||.||||||||||||.|.|.....|.||.:..|||||||:|.|...|||||:||.
Human   200 SPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQ 264

  Fly   256 ILAGLSADEKSRLDLGMAADYKYLTGGNSITCEGRDDAAEFSDIRSAMKVLLFSDQEIWEIIKLL 320
            :.|.....|...|.|..|.|:.|.:.|...:.||.|||.:|...|.|..:|...:.....|.|::
Human   265 LCAAAGLPEFKELALTSAEDFFYTSQGGDTSIEGVDDAEDFEKTRQAFTLLGVKESHQMSIFKII 329

  Fly   321 AALLHCGNIKYKATVVDNLDATEIPEHINVERVAGLLGLPIQPLIDALTRRTLFAHGETVVSTLS 385
            |::||.|::..:|. .|....:..|:.:.:.....|||:....:...|..|.|....||.|.|:|
Human   330 ASILHLGSVAIQAE-RDGDSCSISPQDVYLSNFCRLLGVEHSQMEHWLCHRKLVTTSETYVKTMS 393

  Fly   386 RDQSVDVRDAFVKGIYGRMFVHIVRKINTAIFKPRGTS---RNAIGVLDIFGFENFDQNSFEQFC 447
            ..|.::.|:|..|.||.::|..||..||.|:.    ||   .:.||||||:|||.|:.|||||||
Human   394 LQQVINARNALAKHIYAQLFGWIVEHINKALH----TSLKQHSFIGVLDIYGFETFEVNSFEQFC 454

  Fly   448 INYANENLQQFFVQHIFKLEQEEYNHEAINWQHIEFVDNQDALDLIAIKQLNIMALIDEEARFPK 512
            ||||||.|||.|..|:||||||||..|.|.|..|:|.|||..:|||..| |.|:.|:|||.:.||
Human   455 INYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAK-LGILDLLDEECKVPK 518

  Fly   513 GTDQTMLAKLHKTHGSHKNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSPDLLHLVSQ 577
            ||||....||:..|.|.:::.||:.. ||:|.:.|||..|.|.:.|||:|||||...:.::::..
Human   519 GTDQNWAQKLYDRHSSSQHFQKPRMS-NTAFIIVHFADKVEYLSDGFLEKNRDTVYEEQINILKA 582

  Fly   578 STNKFLRQIFAQDIE-----------------------MGAETRKRTPTLSTQFRKSLDALMKTL 619
            |....:..:|..|.:                       |....::...|:..|||.||..||:||
Human   583 SKFPLVADLFHDDKDPVPATTPGKGSSSKISVRSARPPMKVSNKEHKKTVGHQFRTSLHLLMETL 647

  Fly   620 SSCQPFFIRCIKPNELKKPMMFDRGLCCRQLRYSGMMETIRIRRAGYPIRHGFREFVERYRFLIP 684
            ::..|.::||||||:.|.|..||.....:|||..|::|||||..||||.|..:.:|..|||.|:.
Human   648 NATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWAYHDFFNRYRVLVK 712

  Fly   685 GVPPAHRTDCQAATSRICAVVL-----GKSDYQLGHTKVFLKDAHDLFLEQER-DRVLTRKILIL 743
            ....|: ||.:|    ||..||     ....:|.|.||:|.:.....:||:.| |:..|..|:|
Human   713 KRELAN-TDKKA----ICRSVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRTATIMI- 771

  Fly   744 QRSIRGWVYRRRFLRLRAAAITVQRFWKGYAQR----------------KRYRNMRV--GYMR-- 788
            |:::|||:.:.::.||:.|.:|:||:.:|:..|                |.||..|.  .|.|
Human   772 QKTVRGWLQKVKYHRLKGATLTLQRYCRGHLARRLAEHLRRIRAAVVLQKHYRMQRARQAYQRVR 836

  Fly   789 -----LQALIRSRVLSHRFRH-LRGH-IVGLQAHARGYLVRREYGHKMWAVIKIQSHVRRMIAMR 846
                 :||..|:..:...:|. |..| ...:|.|.||::.||.:.....|.|.||...|.:.|.|
Human   837 RAAVVIQAFTRAMFVRRTYRQVLMEHKATTIQKHVRGWMARRHFQRLRDAAIVIQCAFRMLKARR 901

  Fly   847 RYRKLRLEHKQFAE------------VLQL-RKLEEQELLHRGNKHAREIAEQ------------ 886
            ..:.||:|.:. ||            |:|| ||::||      ||..:.::||
Human   902 ELKALRIEARS-AEHLKRLNVGMENKVVQLQRKIDEQ------NKEFKTLSEQLSVTTSTYTMEV 959

  Fly   887 --------HYRDRLHELERREIQEQLENRR----RVEVNMNIINDA-ARKQEE---PVDDGKLVE 935
                    ||:....|.....:||::|:.|    |......|:.|| :|:::|   .|.|.:...
Human   960 ERLKKELVHYQQSPGEDTSLRLQEEVESLRTELQRAHSERKILEDAHSREKDELRKRVADLEQEN 1024

  Fly   936 AMFDFLPDSSSDAPTPHGGRETSVFNDLPHAQNVNQDDIIAPIHISEDEEDLSEFKFQKFAATYF 1000
            |:   |.|...........:....|     |||..:::::.    .|.||:.|.::
Human  1025 AL---LKDEKEQLNNQILCQSKDEF-----AQNSVKENLMK----KELEEERSRYQ--------- 1068

  Fly  1001 QGNVNHQYAKKALKHPLLPLHTQGDQLAAQALWITILRFT-GDMPEPKYHTMDRMDTTSVMSKVT 1064
              |:..:|::         |..:.|.|..:   :||::.| |....|...:....| ::..|..|
Human  1069 --NLVKEYSQ---------LEQRYDNLRDE---MTIIKQTPGHRRNPSNQSSLESD-SNYPSIST 1118

  Fly  1065 ATLGRNFIRSKEFQEAQLMGL-----DPDAFLKQKPRSIRHKLVSLTLKRKNKLGEDVRRRLQDD 1124
            :.:|..   ....|:.:.:||     |...|||     ::.::..|..:|| ||...:.:|.|.|
Human  1119 SEIGDT---EDALQQVEEIGLEKAAMDMTVFLK-----LQKRVRELEQERK-KLQVQLEKREQQD 1174

  Fly  1125 --EYTADSYQSWLQSRPTSNLEKLHFIIGHGILRAELRDEIYCQICKQLTNNPLKSSHARGWILL 1187
              :..|:..|:.:...|.::|..      :.:.|.||..|          |..||:.
Human  1175 SKKVQAEPPQTDIDLDPNADLAY------NSLKRQELESE----------NKKLKND-------- 1215

  Fly  1188 SLCVGCFAPSEKFVNYLRAFIREGPPGYAPYCEERLKRTFNNGTRNQPPSWL-----------EL 1241
                         :|.||..:.:             :.|.||.:...|.|:.           ||
Human  1216 -------------LNELRKAVAD-------------QATQNNSSHGSPDSYSLLLNQLKLAHEEL 1254

  Fly  1242 QATKSKKPIMLPITFMDGNTKTLLADSATTARELCNQLSD-KISLKDQFGFSLYIALFDKVSSLG 1305
            :..|.:..|:        .|:.:.||.    |.|..:.:: .|:.:               ||..
Human  1255 EVRKEEVLIL--------RTQIVSADQ----RRLAGRNAEPNINAR---------------SSWP 1292

  Fly  1306 SGGDHV--MDAISQCEQYAKEQGAQERNAPWRLFFRKEIFAPWHEPTHDQVATNLIYQ---QVVR 1365
            :...||  .|||   |.|........:...|...             ::.....|.||   ||.|
Human  1293 NSEKHVDQEDAI---EAYHGVCQTNSKTEDWGYL-------------NEDGELGLAYQGLKQVAR 1341

  Fly  1366 --GVKFGEYRCDKEEDLAMIAAQQYFIEYSTDMSME---RLFTLLPNFIPDFCLSGVDKAIERWA 1425
              ..:......:.||::..:.||...::...|...:   :...|.|....:|   ||.:.|.|..
Human  1342 LLEAQLQAQSLEHEEEVEHLKAQLEALKEEMDKQQQTFCQTLLLSPEAQVEF---GVQQEISRLT 1403

  Fly  1426 ALVLQAYKKSYYVKDKIAPL-----KIKEDIVSYAKYKWPLLFS--------RFYEAYRNSGPNL 1477
                   .::..:|:.:..|     |:|:.:..|.|....|..:        :.:|..|......
Human  1404 -------NENLDLKELVEKLEKNERKLKKQLKIYMKKAQDLEAAQALAQSERKRHELNRQVTVQR 1461

  Fly  1478 PKNDVIIAVNWTGVYVVDDQEQVLLELSFPEITAVSSQK---TNKVFTQTFSLSTVRGEEFTFQS 1539
            .:.|      :.|:.....:::.||..:.  :|.:..|.   |............:|..::|
Human  1462 KEKD------FQGMLEYHKEDEALLIRNL--VTDLKPQMLSGTVPCLPAYILYMCIRHADYT--- 1515

  Fly  1540 PNAEDIR--DLVVYFLDGLKKRSKYVIALQDYRAPSDGTSF-----------LSFFKGDLIILED 1591
              .:|::  .|:...::|:||      .|:.:....:.|||           |..:.||    |.
Human  1516 --NDDLKVHSLLTSTINGIKK------VLKKHNDDFEMTSFWLSNTCRLLHCLKQYSGD----EG 1568

  Fly  1592 ESCGESVLNNGWCIGRCDRSQERGDFPAETVYVLPTLSKPPQDIL---------------ALFNI 1641
            .....:...|..|:...|.::.|......::.:...|.|..:.:|               .|..:
Human  1569 FMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGVLQPMIVSAMLENESIQGLSGV 1633

  Fly  1642 EEAHHGRRLSMASNG-------GAVEPRDRPHTLM-EYALD 1674
            :...:.:|.|..::|       ..:...:..||:| :..||
Human  1634 KPTGYRKRSSSMADGDNSYCLEAIIRQMNAFHTVMCDQGLD 1674

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ck	NP_001285949.1	MYSc	64..733	CDD:214580	295/703 (42%)
		MYSc_Myo7	77..721	CDD:276832	286/677 (42%)
		MyTH4	<1138..1245	CDD:214535	19/117 (16%)
		B41	1251..1467	CDD:214604	41/239 (17%)
		FERM_B-lobe	1355..1439	CDD:271216	18/91 (20%)
		FERM_C1_MyoVII	1461..1559	CDD:270019	16/110 (15%)
		SH3	1560..1624	CDD:302595	12/74 (16%)
		MyTH4	1701..1849	CDD:214535
		B41	1856..2068	CDD:214604
		FERM_M	1966..2068	CDD:278785
		FERM_C2_MyoVII	2064..2159	CDD:270020
MYO5B	NP_001073936.1	Requires for interaction with LIMA1. /evidence=ECO:0000269\|PubMed:29880681	21..40
		MYSc	64..760	CDD:214580	294/701 (42%)
		MYSc_Myo5	83..749	CDD:276831	286/677 (42%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	596..630		1/33 (3%)
		Actin-binding. /evidence=ECO:0000255	640..662		11/21 (52%)
		IQ	860..881	CDD:197470	7/20 (35%)
		CCDC144C	895..1172	CDD:291576	72/328 (22%)
		RILP-like	<980..1080	CDD:304877	26/131 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1093..1123		6/30 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1166..1192		5/25 (20%)
		Myo5b_CBD	1474..1845	CDD:271261	36/218 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100101
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810