Protein Alignment spel1 and Msh2

DIOPT Version :10

• Sequence 1: NP_523565.2
• Gene: spel1 / 34842
• FlyBaseID: FBgn0015546
• Length: 917
• Species: Drosophila melanogaster
• Sequence 2: NP_112320.2
• Gene: Msh2 / 81709
• RGDID: 620786
• Length: 933
• Species: Rattus norvegicus
• Alignment Length: 934
• Identity: 389/934 - (41%)
• Similarity: 585/934 - (62%)
• Gaps: 38/934 - (4%)

  Fly    13 TLNMDTNARRNFIKFHAKLGEKPATTVRFFDHTDRYTVHGSDDCELVAKIVYKSTAFIGALLPDD 77
            ||.::..|...|::|...:.|||:||||.||..|.||.|| :|..|.|:.|:|:...|..:.|..
  Rat     8 TLQLEGAAEVGFVRFFEGMPEKPSTTVRLFDRGDFYTAHG-EDALLAAREVFKTQGVIKYMGPAG 71

  Fly    78 KKETLQFVSMSKGNFELAVRELLLVRNYRVEVYVKN--------SSDWEIEYRGSPGNLLQFEDI 134
            .| |||.|.:||.|||..|::|||||:|||||| ||        .:||.:.|:.|||||.|||||
  Rat    72 AK-TLQTVVLSKMNFESFVKDLLLVRHYRVEVY-KNKAGNKASKENDWYLAYKASPGNLSQFEDI 134

  Fly   135 LFSNKEVLVGNSIISL-LVKLDGGGQRRVGVASVEQNDCKFQLLEFLDDDFFTELEATVVLLGPK 198
            ||.|.::.....|:.: |..:|  |||:|||..|:....|..|.||.|:|.|:.|||.::.:|||
  Rat   135 LFGNNDMSTSIGIMGIKLSTVD--GQRQVGVGYVDSTQRKLGLCEFPDNDQFSNLEALLIQIGPK 197

  Fly   199 ECLLPSIE--GEYSAVKTLLDRNGVMITMPKK--SGDNDLLQDLNRLLRFAKGQQEDATGLKELQ 259
            ||:||..|  |:...::.::.|.|::||..|:  ....|:.|||||||:..||:|.::..|.|::
  Rat   198 ECILPGGETAGDMGKLRQVIQRGGILITERKRIDFSTKDIYQDLNRLLKGRKGEQMNSAVLPEME 262

  Fly   260 LQLASNALKTAIKYLDLVNDAGNLGHYEIKQLDLNRFVHLDSAAVAALNIMPKPGTHPSMPSYRW 324
            .|:|.::|...||:|:|::|..|.|.:|:...|.::::.||.|||.|||:. :.....:..|
  Rat   263 NQVAVSSLSAVIKFLELLSDDSNFGQFELATFDFSQYMKLDMAAVRALNLF-QGSVEDTTGS--- 323

  Fly   325 QSVLGVLDHCRTPQGHRLMGQWVKQPLRSRNILNDRHNIVQCLLESPDTMETLSLDYLKRIPDIL 389
            ||:..:|:.|:|.||.||:.||:||||..:|.:.:|.|:|:..:|..:..::|..|.|:|.||:.
  Rat   324 QSLAALLNKCKTAQGQRLVSQWIKQPLMDKNRIEERLNLVEAFVEDSELRQSLQEDLLRRFPDLN 388

  Fly   390 MLTKKLMRRKANLQDLFRIYQVILRTPKILKVLHELD---NSTIESVICAPFKSFLKDLTGLKQM 451
            .|.||..|:.|||||.:|:||.:.:.|.:::.|.:..   .:.:.:|...|......|.:..::|
  Rat   389 RLAKKFQRQAANLQDCYRLYQGVNQLPNVIQALEKYQGRHQALLLAVFVTPLTDLRSDFSKFQEM 453

  Fly   452 VEQVVDFEAIERGEYLVKASFDSRLMELQQMMTELYSKMEELQFKCSQELNLDGKNQVKLESVAK 516
            :|..:|.:.:|..|:|||.|||..|.||:::|..|..||:......::.|.||...|:||:|.|:
  Rat   454 IETTLDMDQVENHEFLVKPSFDPNLSELREVMDGLEKKMQSTLISAARGLGLDPGKQIKLDSSAQ 518

  Fly   517 LGHHFRITVKDDSVLRKNKNYRIVDVIKGGVRFTSDKLEGYADEFASCRTRYEEQQLSIVEEIIH 581
            .|::||:|.|::.|||.|||:..||:.|.||:||:.:|....:|:...:..|||.|.:||:||::
  Rat   519 FGYYFRVTCKEEKVLRNNKNFSTVDIQKNGVKFTNSELSSLNEEYTKNKGEYEEAQDAIVKEIVN 583

  Fly   582 VAVGYAAPLTLLNNELAQLDCLVSFAIAARSAPTPYVRPKMLEEGARELVLEDVRHPCLELQEHV 646
            ::.||..|:..||:.||.||.:||||..:.:||.|||||.:||:|...::|:..||.|:|:|:.|
  Rat   584 ISSGYVEPMQTLNDVLAHLDAVVSFAHVSNAAPVPYVRPVILEKGKGRIILKASRHACVEVQDDV 648

  Fly   647 NFIANSVDFKKEECNMFIITGPNMGGKSTYIRSVGTAVLMAHIGAFVPCSLATISMVDSILGRVG 711
            .||.|.|.|:|::....|||||||||||||||..|..||||.||.||||..|.:|:||.||.|||
  Rat   649 AFIPNDVHFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESAEVSIVDCILARVG 713

  Fly   712 ASDNIIKGLSTFMVEMIETSGIIRTATDKSLVIIDELGRGTSTYEGCGIAWSIAEHLAKETKCFT 776
            |.|:.:||:||||.||:||:.|:|:||..||:||||||||||||:|.|:||:|:|::|.:...|.
  Rat   714 AGDSQLKGVSTFMAEMLETASILRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYIATKIGAFC 778

  Fly   777 LFATHFHEITKLAETLSTVKNCHMAAVADADDFTLLYQVRSGVMEKSFGIQVARLANFPEHVVQN 841
            :|||||||:|.||..:.||.|.|:.|:...:..|:||||::||.::||||.||.|||||.||::.
  Rat   779 MFATHFHELTALASQIPTVNNLHVTALTTEETLTMLYQVKTGVCDQSFGIHVAELANFPRHVIEC 843

  Fly   842 AQEVYNEFED--------EHVDKQKKEDKALLEKIQ---VAIQQLSTAGN--NVDINVEDLTQLV 893
            |::...|.|:        .|.:.|....:..||:.|   :.::.||....  ..|::.|.::..:
  Rat   844 AKQKALELEEFQSIGTSQGHDETQPAAKRRCLEREQGEKIILEFLSKVKQVPFTDLSEESVSVKL 908

  Fly   894 TQFTKDIEQLDSDYFKSVLATSEA 917
            .|...::...::.:...:::..:|
  Rat   909 KQLKAEVLAKNNSFVNEIISRVKA 932

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
spel1	NP_523565.2	MutS_I	24..132	CDD:426350	56/115 (49%)
		MutS	174..890	CDD:440019	310/735 (42%)
Msh2	NP_112320.2	MutS_I	18..132	CDD:426350	56/116 (48%)
		MutS	160..867	CDD:440019	307/710 (43%)
		Interaction with EXO1. /evidence=ECO:0000250	601..671		33/69 (48%)