Protein Alignment spel1 and msh2

DIOPT Version :10

• Sequence 1: NP_523565.2
• Gene: spel1 / 34842
• FlyBaseID: FBgn0015546
• Length: 917
• Species: Drosophila melanogaster
• Sequence 2: XP_002935427.2
• Gene: msh2 / 100380043
• XenbaseID: XB-GENE-977518
• Length: 932
• Species: Xenopus tropicalis
• Alignment Length: 936
• Identity: 399/936 - (42%)
• Similarity: 588/936 - (62%)
• Gaps: 48/936 - (5%)

  Fly    14 LNMDTNARRNFIKFHAKLGEKPATTVRFFDHTDRYTVHGSDDCELVAKIVYKSTAFIGALLPDDK 78
            |:||:.|...||.|:..:.|||.||||.||..|.|||||. |....||.|:|:...|..|....|
 Frog     9 LSMDSGAENGFISFYQSMPEKPDTTVRVFDRNDYYTVHGG-DAIFAAKEVFKTNGVIKYLGSGSK 72

  Fly    79 KETLQFVSMSKGNFELAVRELLLVRNYRVEVYVKNSS--------DWEIEYRGSPGNLLQFEDIL 135
            |  |:.|.:||.|||..|::|||||.|||||| ||.|        ||::.::.|||||.|||:||
 Frog    73 K--LESVVLSKMNFESVVKDLLLVRQYRVEVY-KNKSGGKSSKENDWQLAFKASPGNLTQFEEIL 134

  Fly   136 FSNKEVLVGNSIISL-LVKLDGGGQRRVGVASVEQNDCKFQLLEFLDDDFFTELEATVVLLGPKE 199
            |.|.::.....::.: ||..:  |||.|||..|:....|..:.||.|:|.|:.|||.:|.:||||
 Frog   135 FGNSDMSTAVGVVGIKLVSAE--GQRLVGVGYVDSTLRKLGVCEFPDNDQFSNLEALLVQIGPKE 197

  Fly   200 CLLP--SIEGEYSAVKTLLDRNGVMITMPKKS--GDNDLLQDLNRLLRFAKGQQEDATGLKELQL 260
            |::|  ...|:...::.::.|.|::||..||:  ...|.:|||||||:..||:|..:..|.|::.
 Frog   198 CVMPGGDTAGDMGKLRQIVKRGGILITDRKKAEFTTKDSVQDLNRLLKAKKGEQVTSAALPEMEK 262

  Fly   261 QLASNALKTAIKYLDLVNDAGNLGHYEIKQLDLNRFVHLDSAAVAALNIMP-----KPGTHPSMP 320
            |:|.:||.:.:|||:|::|..|.|.:.:...||::::.||:|||.|||:.|     ..||
 Frog   263 QVAMSALASVMKYLELLSDESNFGQFVMTNFDLSQYMKLDNAAVGALNLFPGSAEDTSGT----- 322

  Fly   321 SYRWQSVLGVLDHCRTPQGHRLMGQWVKQPLRSRNILNDRHNIVQCLLESPDTMETLSLDYLKRI 385
                ||:.|:|:.|:||||.||:.||:||||..:|.:.:|.|:|:..:...:..:.|..|.|:|.
 Frog   323 ----QSLAGLLNKCKTPQGQRLVNQWIKQPLMDKNRIEERLNLVEAFVTDAELRQCLQEDLLRRF 383

  Fly   386 PDILMLTKKLMRRKANLQDLFRIYQVILRTPKILKVLHELDNS---TIESVICAPFKSFLKDLTG 447
            ||:..|.||..|:.|||||.:|:||.:.:.|.:::.:.:.:.:   .:.:|...|......|.:.
 Frog   384 PDLNRLAKKFQRQTANLQDCYRLYQAVNQLPGVIQAIEKYEGTHQMLLLAVFATPLSDLSSDFSK 448

  Fly   448 LKQMVEQVVDFEAIERGEYLVKASFDSRLMELQQMMTELYSKMEELQFKCSQELNLDGKNQVKLE 512
            .::|:|..:|.:.:|..|:|||||||..|.||::.|.||...|:......::||.||....:|||
 Frog   449 FQEMIETTLDMDQVENHEFLVKASFDPNLTELREKMNELEKNMQAALNGAARELGLDAGKSIKLE 513

  Fly   513 SVAKLGHHFRITVKDDSVLRKNKNYRIVDVIKGGVRFTSDKLEGYADEFASCRTRYEEQQLSIVE 577
            |.:::||.||:|.|::..||.||.:..:|:.|.|||||:.||...::|:...|..|||.|.:||:
 Frog   514 SNSQVGHFFRVTCKEEKALRNNKKFTTIDIQKNGVRFTNGKLSSLSEEYMRNREEYEEAQNAIVK 578

  Fly   578 EIIHVAVGYAAPLTLLNNELAQLDCLVSFAIAARSAPTPYVRPKMLEEGARELVLEDVRHPCLEL 642
            |||.::.||..|:..||:.:||||..||||..:.|||.|||||.:||:|..:::|:..||||:|:
 Frog   579 EIISISAGYVDPIQTLNDVIAQLDAAVSFAYVSNSAPVPYVRPVILEKGQGKIMLQSARHPCIEM 643

  Fly   643 QEHVNFIANSVDFKKEECNMFIITGPNMGGKSTYIRSVGTAVLMAHIGAFVPCSLATISMVDSIL 707
            |:.|.||.|.:.|:||:....|||||||||||||||..|..||||.||:||||..|.||:||.||
 Frog   644 QDDVAFIPNDITFEKEKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGSFVPCDSAEISIVDCIL 708

  Fly   708 GRVGASDNIIKGLSTFMVEMIETSGIIRTATDKSLVIIDELGRGTSTYEGCGIAWSIAEHLAKET 772
            .||||.|:.:||:||||.||:||:.|:|:||:.||:||||||||||||:|.|:||:|:|:::.:.
 Frog   709 ARVGAGDSQLKGVSTFMAEMLETASILRSATENSLIIIDELGRGTSTYDGFGLAWAISEYISTKI 773

  Fly   773 KCFTLFATHFHEITKLAETLSTVKNCHMAAVADADDFTLLYQVRSGVMEKSFGIQVARLANFPEH 837
            |.|.:|||||||:|.||:.:.||.|.|:.|:...|..|:||:::.||.::||||.||.|||||:|
 Frog   774 KAFCMFATHFHELTALADQVPTVNNLHVTALTTEDTLTMLYRIKRGVCDQSFGIHVAELANFPKH 838

  Fly   838 VVQNAQE---VYNEF---------EDEHVDKQKKEDKALLEKIQVAIQQLSTAGNNVDINVEDLT 890
            |::.|:|   ...||         :||...|::.::|...|||.........|....:::.|::.
 Frog   839 VIETAKEKALQLEEFQFVGNPDDCDDEPTRKRRCKEKEEGEKIIQDFLSRVKALPLTEMSEEEIK 903

  Fly   891 QLVTQFTKDIEQLDSDYFKSVLATSE 916
            ..:.||..|:...::.:...|::.::
 Frog   904 SKLQQFRSDVLARNNSFVHEVISRTK 929

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
spel1	NP_523565.2	MutS_I	24..132	CDD:426350	57/115 (50%)
		MutS	174..890	CDD:440019	318/739 (43%)
msh2	XP_002935427.2	MutS_I	18..131	CDD:426350	57/116 (49%)
		MutS	152..852	CDD:440019	316/710 (45%)