Protein Alignment nAChRalpha5 and chrna2b

DIOPT Version :10

• Sequence 1: NP_001356885.1
• Gene: nAChRalpha5 / 34826
• FlyBaseID: FBgn0028875
• Length: 837
• Species: Drosophila melanogaster
• Sequence 2: XP_697298.4
• Gene: chrna2b / 568849
• ZFINID: ZDB-GENE-041001-99
• Length: 550
• Species: Danio rerio
• Alignment Length: 544
• Identity: 206/544 - (37%)
• Similarity: 288/544 - (52%)
• Gaps: 47/544 - (8%)

  Fly   294 IFLLIYLNLSAKVCLA--------GYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQII 350
            :||.|.| |...:|.:        .:.|..|...|.|.||...|||.|.||.:.:.|||::.|:|
Zfish     9 VFLRITL-LCTIICESISCCEKTHSHAEDELFKKLFDGYNKWSRPVPNTSDVVIVKFGLSIAQLI 72

  Fly   351 DVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQT 415
            |||||||::.||||||.||||..|||..|||..|..:|:|...||.||:::||:||..|..|:.|
Zfish    73 DVDEKNQMMTTNVWLKQEWNDYKLRWKPSDYDNVTSIRVPSELIWVPDIVLYNNADGEFAVTHMT 137

  Fly   416 NVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGGDISSY 480
            ...:.:.|...:|||.|:||:|.||:|:||||.|.|:|||||||||..::||:..:.| .|:..|
Zfish   138 KAHLFHTGKVRWVPPAIYKSSCSIDVTFFPFDQQNCKMKFGSWTYDKAKIDLERIENT-VDLKDY 201

  Fly   481 VLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLP 545
            ..:|||.::...|..|...|:||.|.|.|||:..||||..|:|..||||||:||:.:.:|.|.||
Zfish   202 WESGEWAIINAVGTYNTKKYDCCHEIYPDITYFFIIRRLPLFYTINLIIPCLLISCLTVLVFYLP 266

  Fly   546 PDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHR 610
            .|.|||::|.:::||||||||.::.|.:|:||..:||:|.|....|..|..|:|.|:.:||.|||
Zfish   267 SDCGEKITLCISVLLSLTVFLLLITEIIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHHR 331

  Fly   611 NADTHEMSEWIRIVFLCWLPWILRMSRP---------------------GRPLILEFPTTPC--- 651
            :..||:|..|:..|||..:|..|.|.||                     .||......|:..
Zfish   332 SPSTHKMPCWVHSVFLDLIPRWLFMRRPAPDSRRRQKLLLLQRQVPGMTARPANASLSTSTSWFR 396

  Fly   652 ---SDTSSERKHQILSDVELKERSSKSLLANVLDIDDDFRHNCRP--MTPGGTLPHNPAFYRTVY 711
               |......:|....|:||      ..|::.:.:.......| |  :||.......|| .|...
Zfish   397 GQNSGEDESCRHFSYKDLEL------GTLSSAISLSLQASSPC-PLGLTPPPLQKFGPA-PRLEV 453

  Fly   712 GQGDDGSIGPIGSTRMPDAVTHHTCIKSSTEYELGLILKEIRFITDQLRKDDECNDIANDWKFAA 776
            |.......|....|:..|.:..|..........:...|:.:.:|.|.||.:|....:..|||:.|
Zfish   454 GTASRQLSGRANVTKRLDNMIPHDNPGFPLSPSVLQALEGVHYIADHLRAEDADFSVKEDWKYVA 518

  Fly   777 MVVDRLCLIIFTMFTILATIAVLL 800
            ||:||:.|.:|.:..:|.||.:.|
Zfish   519 MVIDRIFLWMFIIVCLLGTIGLFL 542

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
nAChRalpha5	NP_001356885.1	LIC	313..802	CDD:455710	200/517 (39%)
chrna2b	XP_697298.4	LIC	6..543	CDD:455710	206/544 (38%)
		LGIC_ECD_nAChR_A2	35..241	CDD:349816	102/206 (50%)
		Cys-loop	159..173	CDD:349816	9/13 (69%)