DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rk and LGR5

DIOPT Version :9

Sequence 1:	NP_476702.1	Gene:	rk / 34819	FlyBaseID:	FBgn0003255	Length:	1360	Species:	Drosophila melanogaster
Sequence 2:	NP_003658.1	Gene:	LGR5 / 8549	HGNCID:	4504	Length:	907	Species:	Homo sapiens

Alignment Length:	984	Identity:	283/984 - (28%)
Similarity:	434/984 - (44%)	Gaps:	134/984 - (13%)

- Green bases have known domain annotations that are detailed below.

  Fly   123 QLSTASTATDVIAGKRTLHSICERWLQKHCHC--TGSLEVLRLSCRGIGILAVPVNLPNEVVVLD 185
            ||:|        .|......:..|....||||  .|.: :||:.|..:|:..:|.||......||
Human    17 QLAT--------GGSSPRSGVLLRGCPTHCHCEPDGRM-LLRVDCSDLGLSELPSNLSVFTSYLD 72

  Fly   186 LGNNNLTKLEANSFFMAPNLEELTLSDNSIINMDPNAFYGLAKLKRLSLQNCGLKSLPPQSFQGL 250
            |..||:::|..|.......||||.|:.|::..:...||.||..||.|.|||..|:.:|.::.|.|
Human    73 LSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNL 137

  Fly   251 AQLTSLQLNGNALVSLDGDCLGHLQKLRTLRLEGNLFYRIPTNALAGLRTLEALNLGSNLLTIIN 315
            ..|.||:|:.|.:..:...|...|..||.|.|:.|....||..|...|..|:|:.|..|.:..|.
Human   138 RSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIP 202

  Fly   316 DEDFPRMPNLIVLLLKRNQIMKISAGALKNLTALKVLELDDNLISSLPEGLSKLSQLQELSITSN 380
            |..|..:.:|:||.|..|:|..:.......|.:|:.|:|:.|.:...|..:..||.|:||...||
Human   203 DYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSN 267

  Fly   381 RLRWINDTELPRSMQMLDMR--ANPLSTISPGAFRGMSKLRKLILSDVRTLRSFPELEACHALEI 443
            .:|.|.:.....:..::.:.  .||:..:...||:.:.:||.|.|:....:..||:|.....||.
Human   268 NIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLES 332

  Fly   444 LKLDRAGIQEVPANLCRQTPRLKSLELKTNSLKRIPNLSSCRDLRLLDLSSNQIEKIQGKPFNGL 508
            |.|..|.|..:|..:|.|.|.|:.|:|..|.|:.:|:.|.|:.|:.:||..|:|.:|:
Human   333 LTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIK------- 390

  Fly   509 KQLNDLLLSYNRIKALPQDAFQGIPKLQLLDLEGNEISYIHKEAFSGFTALEDLNLGNNIFPELP 573
                             .|.||.:..|:.|:|..|:|:.||..|||...:|..|:|.:|:....|
Human   391 -----------------VDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFP 438

  Fly   574 ESGLRALLHLKTFNNPKLREFPPPDTFPRIQTLILSYAYHCCAFLPLVAMSSQKKTSQVQEAVLF 638
            .:||..|.|||...|..|:.....:.||.::.:.:.|||.||||
Human   439 ITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAF--------------------- 482

  Fly   639 PSDAEFDMTLWNNSMMNIWPQMHNLSKQLGASMHDPWETAINFNEEQLQTQTGGQIATSYMEEYF 703
                            .:....:.:|.|        |....|.:.:.|..:..|      |.:..
Human   483 ----------------GVCENAYKISNQ--------WNKGDNSSMDDLHKKDAG------MFQAQ 517

  Fly   704 EEHDVSGPATGYGFGTGLFSGMSTEDF---------QPGSVQCLPMPGPFLPCADLFDWWTLRCG 759
            :|.|:                   |||         ...||||.|.||||.||..|.|.|.:|.|
Human   518 DERDL-------------------EDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIG 563

  Fly   760 VWVVFLLSLLGNGTVVFVLLCSRSKMDVPRFLVCNLAAADFFMGIYLGILAIVDAATLGEFRMFA 824
            ||.:.:|:|..|..|...:..|...:...:.|:..:||.:...|:...:||.|||.|.|.|....
Human   564 VWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHG 628

  Fly   825 IPWQMSVLCQLSGFLAVLSSELSVYTLAVITLERNYAITHAIHLNKRLSLKQAGYIMSVGWVFAL 889
            ..|:..|.|.:.|||::.:||.||:.|.:..|||.:::.::.....:........|:.:..:.||
Human   629 AWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLAL 693

  Fly   890 IMALMPLVGVSDYRKFAVCLPFETTTGPASLTYVISLMFINGCAFLTLMGCYLKMYWAIRGSQAW 954
            .||.:||:|.|.|....:|||..... |:::.|:::|:.:|...||.:...|.|:|..:......
Human   694 TMAAVPLLGGSKYGASPLCLPLPFGE-PSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLE 757

  Fly   955 NTNDSRIAKRMALLVFTDFLCWSPIAFFSITAIFGLQLISLEQAKIFTVFVLPLNSCCNPFLYAI 1019
            |..|..:.|.:|||:||:.:...|:||.|.:::..|..||.|..|...:.|:||.:|.||.||.:
Human   758 NIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYIL 822

  Fly  1020 MTKQFKKDCVTL---------CKH-----FEESRVVGGGGPGGRGAVARTKRG---DLPPPLLPA 1067
            ....||:|.|:|         .||     .....|........:..|..|...   ||||..:|:
Human   823 FNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPS 887

  Fly  1068 AAVAHPPGC 1076
            .|......|
Human   888 PAYPVTESC 896

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rk	NP_476702.1	leucine-rich repeat	158..179	CDD:275380	7/20 (35%)
		LRR_RI	180..>383	CDD:238064	69/202 (34%)
		leucine-rich repeat	183..204	CDD:275380	7/20 (35%)
		LRR_8	203..263	CDD:290566	25/59 (42%)
		leucine-rich repeat	205..228	CDD:275380	10/22 (45%)
		leucine-rich repeat	229..252	CDD:275380	10/22 (45%)
		LRR_8	252..311	CDD:290566	20/58 (34%)
		leucine-rich repeat	253..276	CDD:275380	7/22 (32%)
		leucine-rich repeat	277..300	CDD:275380	9/22 (41%)
		LRR_8	299..359	CDD:290566	18/59 (31%)
		leucine-rich repeat	301..324	CDD:275380	7/22 (32%)
		leucine-rich repeat	325..348	CDD:275380	7/22 (32%)
		LRR_RI	327..567	CDD:238064	71/241 (29%)
		leucine-rich repeat	349..371	CDD:275380	6/21 (29%)
		LRR_8	370..425	CDD:290566	16/56 (29%)
		leucine-rich repeat	372..393	CDD:275380	7/20 (35%)
		leucine-rich repeat	394..415	CDD:275380	4/22 (18%)
		leucine-rich repeat	418..486	CDD:275380	25/67 (37%)
		leucine-rich repeat	441..464	CDD:275378	9/22 (41%)
		LRR_8	487..545	CDD:290566	13/57 (23%)
		leucine-rich repeat	487..510	CDD:275380	6/22 (27%)
		leucine-rich repeat	511..534	CDD:275380	3/22 (14%)
		LRR_8	533..589	CDD:290566	21/55 (38%)
		leucine-rich repeat	535..558	CDD:275380	10/22 (45%)
		leucine-rich repeat	559..580	CDD:275380	7/20 (35%)
		7tm_4	764..>892	CDD:304433	34/127 (27%)
		7tm_1	770..1017	CDD:278431	74/246 (30%)
LGR5	NP_003658.1	LRRNT	33..70	CDD:214470	12/37 (32%)
		leucine-rich repeat	68..91	CDD:275380	7/22 (32%)
		PLN00113	71..>426	CDD:215061	120/378 (32%)
		leucine-rich repeat	92..115	CDD:275380	10/22 (45%)
		leucine-rich repeat	116..139	CDD:275380	10/22 (45%)
		leucine-rich repeat	140..163	CDD:275380	7/22 (32%)
		leucine-rich repeat	164..187	CDD:275380	9/22 (41%)
		leucine-rich repeat	188..211	CDD:275380	7/22 (32%)
		leucine-rich repeat	212..235	CDD:275380	7/22 (32%)
		leucine-rich repeat	236..258	CDD:275380	6/21 (29%)
		leucine-rich repeat	259..282	CDD:275380	7/22 (32%)
		leucine-rich repeat	283..304	CDD:275380	4/20 (20%)
		leucine-rich repeat	307..353	CDD:275378	16/45 (36%)
		leucine-rich repeat	354..375	CDD:275378	8/20 (40%)
		leucine-rich repeat	376..389	CDD:275378	5/12 (42%)
		leucine-rich repeat	400..423	CDD:275378	10/22 (45%)
		leucine-rich repeat	445..459	CDD:275378	6/13 (46%)
		7tmA_LGR5	558..831	CDD:320485	85/273 (31%)
		TM helix 1	559..585	CDD:320485	8/25 (32%)
		TM helix 2	592..617	CDD:320485	6/24 (25%)
		TM helix 3	637..667	CDD:320485	12/29 (41%)
		TM helix 4	679..701	CDD:320485	6/21 (29%)
		TM helix 5	721..750	CDD:320485	8/28 (29%)
		TM helix 6	759..789	CDD:320485	11/29 (38%)
		TM helix 7	799..824	CDD:320485	10/24 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2087
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20807
Inparanoid	1	1.050	311	1.000	Inparanoid score	I2595
Isobase	1	0.950	-	0	Normalized mean entropy	S5677
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D171884at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm41437
orthoMCL	1	0.900	-	-		OOG6_106088
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X2481
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	11	10.680