Protein Alignment Smg5 and EBS1

DIOPT Version :10

• Sequence 1: NP_609685.1
• Gene: Smg5 / 34804
• FlyBaseID: FBgn0019890
• Length: 1177
• Species: Drosophila melanogaster
• Sequence 2: NP_010492.3
• Gene: EBS1 / 851787
• SGDID: S000002614
• Length: 884
• Species: Saccharomyces cerevisiae
• Alignment Length: 1136
• Identity: 197/1136 - (17%)
• Similarity: 339/1136 - (29%)
• Gaps: 482/1136 - (42%)

  Fly    89 IFKDYQSVGKKVREVMWRRGYYEFIAFVKKNWHKQCQDAATAQENMQKLERFLCAGIFNYKRLSA 153
            |||.:|         |||    .:|.|.|:|..:|.:.....:.|.:.|:.|.....| |..:..
Yeast    92 IFKWFQ---------MWR----NYILFEKENKKQQTKFIDFRKMNSKMLKFFKTVQNF-YVNVIN 142

  Fly   154 RMEEIYDLDLKYLIDFSIISDGYISDM-----IGD-----TMESSHSGTHAVDKSLEAVSFALDT 208
            .:.:.||:.:  |:...||.|..:||:     :||     |..||....|.:.          ..
Yeast   143 TVYKKYDISV--LLPKRIIQDLKLSDIENTTNVGDILAVKTFNSSSPLAHLIP----------TL 195

  Fly   209 IHSSLLSLGDLHRY--FLDFRIDSKLSISKEQVAKYYLEAFKLN----PAIGMAQNQLGTLHYGQ 267
            .|..||.||..:||  .|: .|.:|.|||.   .|..|:.|:|.    |:.|...:|.|.: :.|
Yeast   196 FHRCLLFLGTAYRYKTLLE-EISNKYSISN---FKKSLDFFRLASLVLPSAGETYSQAGAI-FLQ 255

  Fly   268 NHDLD-STYHYLYSLVCIIPFELSENNLNKLFAKHTEYLERMDPEKLDFNIEDFFARFYLIVDIF 331
            ..:|. :.::::..::..:|..:|..|...|...:...|.|              :....|::.:
Yeast   256 TGNLGIAVFNFVKGMMTKMPSPVSIKNFGALMVDNKSSLNR--------------SLHTTIMNTY 306

  Fly   332 FFDKEVPDFNALCHCVLIDLRKILCSQQLRVPEPSIFKIVSILFFCLSKLKMINSPKVYSLHAFL 396
            ..:.:.|                      |.|...|.:     |:.|.....:.||
Yeast   307 LQESKGP----------------------RTPAKEILE-----FYFLGLFGSVWSP--------- 335

  Fly   397 VAACSDLMDACIVSIEQTILARAQDNERFQAEYEERFQEFDTVVRKSRNEYKNWLAAVGECERKD 461
                                                               .:|        |.|
Yeast   336 ---------------------------------------------------TSW--------RDD 341

  Fly   462 KKLMPRPHSLKDCSSDSRDSQHSGEEAKTQEAADGDKIGEAVSTRSSDEAKE--SDLKTPLSCKS 524
            .|    |:.|           ::|.:.:..|.|    :.|.:|.|..:..|.  .:|...:.
Yeast   342 TK----PNQL-----------NNGIKLRHLENA----LYETMSARYLNNIKTIFHNLIITIG--- 384

  Fly   525 KRRGMRLRRRRRKIAQSDDSSCYDSESELDTDFYSDEEDYTDLSSNFDTDFSDIDAADPGEPCGE 589
               |..|..:||.          |..::...|..|:|.||.:.:..:
Yeast   385 ---GFHLLLKRRS----------DVSAKTLKDLRSNELDYLNFAFKY------------------ 418

  Fly   590 ERYAAANYSKKERKAGGGGGGGVGGVVYSDNEDVVIEEEKIVYPNEVERRSNSQEADSEELLRSF 654
                                  :..::                 |::.:.|.|:..:..|:|...
Yeast   419 ----------------------IAHIL-----------------NDIVKESWSENPEVSEILGMV 444

  Fly   655 EVLQLNFKSAEDAQSKPVAPPPVSFSEPPKKLVYRNKYTKINPNIVIDCLHNEPTIRALKMLFDW 719
            .                                            :|:|               |
Yeast   445 R--------------------------------------------IINC---------------W 450

  Fly   720 LRINPEILIGCYHSNPEFVHKIMKLLNYCNIDIFTRK---------------VYFERDLLTTANV 769
            ::.||.:| ....||.|||:.:..|:|    ||..:|               .:||.||
Yeast   451 IKANPMVL-QYSQSNLEFVNALAYLIN----DIVKKKPSPSFSITEHIPKRTYWFEEDL------ 504

  Fly   770 RDSLVELFDVRANVPLTEDFAFKNFDIFQSTQITLDWETIIR---------------ERVTPQEE 819
                           :.:..:|.||.:....    |:|.|:.               ::::...|
Yeast   505 ---------------MVKGLSFVNFQLSDFD----DYEKILEMDHSLDRLIGNPPLCDKLSASSE 550

  Fly   820 SILRIFKMVDFGFFICK---------QKKFNYRFCVKTRRFTETQKKKQREEKKGGGSRRRERRT 875
            .:||:..:|:....:.:         ..|..|.|..|. .|.||.|...:..|:.....:.:|:.
Yeast   551 MLLRLQAVVNISSQLLQNNNCGVEWSDNKSRYIFNKKI-GFKETVKNSMKTSKQSNEKAKLQRKN 614

  Fly   876 APKT----------NRTKRNE-----GRTRRYS--------DRKNGIVRKSYTSNEEKDTVEECR 917
            .|.|          .|..|:.     ..|..||        |..|  |:.|.|....|..||..
Yeast   615 KPSTTNGSISMADLERQMRSSSLDSFSPTMGYSGSSVPMAPDTFN--VKPSGTITGNKVNVELL- 676

  Fly   918 KMPRKGYLRNRNAEKAAAVITSATGTSVAGGTSASTTTNSVIEKLNLQSS-SGADEERSEAKSKK 981
                |..|..:||:  .|:...:.|.|.|..:|:::|..|..:..|:.|| .....|:|.|||.:
Yeast   677 ----KIELSGQNAD--GAITNISPGYSNAAISSSNSTDESSFDLNNILSSMQQKHAEKSFAKSMQ 735

  Fly   982 -----------CE-----LMGKLWLQNEVETLEEELRDSPAHVVMSPFVVVDSKALTEYNGIVKS 1030
                       |.     :.|.|:...:..:: ..|..:..:..|.|     |.::..|
Yeast   736 GVNEQIPANDVCHQAQRPMQGGLYSSQQPSSM-SSLNSAYQNASMPP-----SASMVSY------ 788

  Fly  1031 LVRTKKCIVLIPNAVLNE----------LDDLKKRSE-------NVRHVIRWLEQEFK---QGNR 1075
                       |...||:          ..:|:.:||       |..:. .||..:::   ..:.
Yeast   789 -----------PYPFLNQQGQGVFPPYNAQNLQWQSEAYSLKSMNFANP-TWLGDQYQTSAPSSA 841

  Fly  1076 HLRAQRDNESQPLSLLKISRKMDRDASVFLQ 1106
            :.:|||....||         |.:|...::|
Yeast   842 YAQAQRQMFQQP---------MQQDVGKYMQ 863

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smg5	NP_609685.1	EST1	99..227	CDD:463062	35/139 (25%)
		EST1_DNA_bind	240..479	CDD:431239	31/243 (13%)
		MSCRAMM_ClfA	<470..>580	CDD:468110	19/111 (17%)
		PIN_Smg5-like	1012..1173	CDD:350232	20/115 (17%)
EBS1	NP_010492.3	EST1	79..214	CDD:463062	38/147 (26%)
		EST1_DNA_bind	228..516	CDD:431239	76/574 (13%)