DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smg5 and Smg6

DIOPT Version :9

Sequence 1:	NP_001285914.1	Gene:	Smg5 / 34804	FlyBaseID:	FBgn0019890	Length:	1177	Species:	Drosophila melanogaster
Sequence 2:	NP_001002764.1	Gene:	Smg6 / 103677	MGIID:	2144117	Length:	1418	Species:	Mus musculus

Alignment Length:	1229	Identity:	209/1229 - (17%)
Similarity:	379/1229 - (30%)	Gaps:	483/1229 - (39%)

- Green bases have known domain annotations that are detailed below.

  Fly    37 ILSQEVKDLYRSLYTASK---QLDD--AKRNVQSVGQLFQHEIEEKRSLLVQLCKQIIFKDYQ-S 95
            :.:.:.::|:|.|..|..   ||.:  ::..:.:.|   ..::.:.|:.|:||.::.|..|.: |
Mouse   572 VRNMQQQELHRLLRVADNQELQLSNLLSRDRISTEG---MEKMAQLRTELLQLYERCILLDIEFS 633

  Fly    96 VGKKVREVMWRRGYYEFIAFVKKNWHKQCQD--AATAQENMQKLERFLCAGIFNYKRLSARMEEI 158
            ..:.|.:::|:..:|:.|    :.:.:..:|  :...::...:|...|..|...:..|..:::..
Mouse   634 DSQNVDQILWKNAFYQVI----EKFRQLLKDPNSENPEQIRNRLLELLDEGSDFFDSLLQKLQVT 694

  Fly   159 YDLDLKYLIDFSIISDGYISDMIGDTMESSHSGTHAVDKSL-EAVSFALDTIHSSLLSLGDLHRY 222
            |...|:..:|                      |.....|.| :.|.:||.:...|::..||:.||
Mouse   695 YKFKLEDYMD----------------------GLAIRSKPLRKTVKYALISAQRSMICQGDISRY 737

  Fly   223 FLDFRIDSKLSISKEQVAKYYLEAFKLNPAIGMAQNQLGTLHYGQNHDLDSTYHYLYSLVCIIPF 287
                |..:..:.:..:...:||:|..:.|..|...|||..|.......||:.|:|:.||....|.
Mouse   738 ----REQANDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPI 798

  Fly   288 ELSENNLNKLF---AKHTEYLERMDPEKLDFNIEDFFARFYLIVDIFFFDKEVPDFNALCHCVLI 349
            ..::.:|..||   .:..|.:|:...|:.|.:                     ||
Mouse   799 LTAKESLMSLFEETKRKAEQMEKKQHEEFDMS---------------------PD---------- 832

  Fly   350 DLRKILCSQQLRVPEPSIFKIVSILFFCLSKLKMINSPK-------------------VYSLHAF 395
                     :.|..:.|.|:.|.   ...::|::...|.                   :.||
Mouse   833 ---------KWRKGKKSTFRHVG---DDTTRLEIWIHPSHSRSAQGTESGKDSEQENGLGSL--- 882

  Fly   396 LVAACSDLMDACIVSI---EQTILARAQDNERFQAEYEERFQEFDTVVRKSRNEYKNWLAAVGEC 457
               :.|||....|:|.   ...:..|. ..|.|.|..|:..:||..:::.|.       :.:|
Mouse   883 ---SPSDLNKRFILSFLHAHGKLFTRI-GMETFPAVAEKVLKEFQVLLQHSP-------SPIG-- 934

  Fly   458 ERKDKKLMP------RPHSLKDCSSDSRDSQHSGEEAKTQEAADGDKIGEAVSTRSSDEAKESDL 516
            ..:..:||.      ....||||.|         ||.::........:|.|:.         |.|
Mouse   935 STRMLQLMTINMFAVHNSQLKDCFS---------EECRSVIQEQAASLGLAMF---------SLL 981

  Fly   517 KTPLSCKSKRRGMRLRRRRRKIAQSDDSSCYDSESELDTDFYSDEEDYTDLSSNFDTDFSDIDAA 581
            ....:|..|                |.:....|..|       |:||..|:
Mouse   982 VQRCTCLLK----------------DSAKAQLSSPE-------DQEDQDDI-------------- 1009

  Fly   582 DPGEPCGEERYAAANYSKKERKAGGGGGGGVGGVVYSDNEDVVIEEEKIVYPNEVERRSNSQEAD 646
                                                                     :.:|...|
Mouse  1010 ---------------------------------------------------------KVSSFVPD 1017

  Fly   647 SEELLRSFEVLQLNFKSAEDAQSKPVAPPPVSFSEPPKKLVYRNKYTKINPNIVIDCLHNEPTIR 711
            .:|||.|.:|.........|..:    |||.|...|
Mouse  1018 LKELLPSVKVWSDWMLGYPDTWN----PPPTSLDLP----------------------------- 1049

  Fly   712 ALKMLFD-WLRINPEILIGCYHSNPEFVHKIMKLLNYCNIDIFTRKVYFERDLLTTANVRDSLVE 775
             |::..| |                      ..|.::|||             ||..|
Mouse  1050 -LQVAVDVW----------------------STLADFCNI-------------LTAVN------- 1071

  Fly   776 LFDVRANVPLTEDFAFKNFDIFQSTQITLDWETIIRERV----TPQEESIL-------------R 823
                ::.|||.:|   .:.|:   |.:.|:.:.::...|    .||:...:             |
Mouse  1072 ----QSEVPLYKD---PDDDL---TLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKR 1126

  Fly   824 IFKMVDFGFFICKQKKFNYRFCVKTRRFTETQKKKQREEKKGGGSRRRERRTAPKTNRTKRNEGR 888
            :..:..|...:|.|::....|                   |||  :.......|.|...:.....
Mouse  1127 VTVLKYFLEALCGQEEPLLAF-------------------KGG--KYVSVAPVPDTMGKEMGSQE 1170

  Fly   889 TRRYSDRKNGIVRKSYTSNEE------KDTVEECR----KMPRKGYLRNRNAEKAAAVITSATGT 943
            .::..|.:..:|.:.:..:.|      :|.:.|.|    .:.||...:.|..||..||:..
Mouse  1171 GKQLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLED---- 1231

  Fly   944 SVAGGTSASTTTNSVIEKLNLQSSSGADEERSEAKSKKCELMGKLWLQNEVETLEEELRDSPAHV 1008
                                                           |:::..:|.|:|..
Mouse  1232 -----------------------------------------------QSQMRQMELEIRPL---- 1245

  Fly  1009 VMSPFVVVDSKALTEYNGIVKSLVRTKKCIVLIPNAVLNELDDLKKR--------------SENV 1059
                |:|.|:....::...:..|:.::|.|:::|..|:||||.|.|.              .|..
Mouse  1246 ----FLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKA 1306

  Fly  1060 RHVIRWLEQEFKQGNRHLRA--QRDNESQPLSLLK--ISRKMDRDASVFLQIAQFCNHLVANHAD 1120
            |..|.:||:.|:..:..|||  .|.||.:.::...  |:.::..:..:.|..   |.|...:.|.
Mouse  1307 RKSIEFLERRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSC---CLHYCKDKAK 1368

  Fly  1121 PHVS-------ELQKSVVTYLSGDNLHEKNRQQQNFSYTGILDSIPVR-------YAQI 1165
            .::.       .|.:.||......||..|          .:..::|||       :||:
Mouse  1369 DYMPTSKEEPIRLLREVVLLTDDRNLRVK----------ALTRNVPVRDIPAFLTWAQV 1417

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smg5	NP_001285914.1	EST1	99..222	CDD:287360	21/125 (17%)
		EST1_DNA_bind	240..478	CDD:287359	54/268 (20%)
		PIN_Smg5	1012..1173	CDD:189054	42/186 (23%)
Smg6	NP_001002764.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	23..86
		EJC-binding motif 1, mediates interaction with the EJC. /evidence=ECO:0000250	39..59
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	102..123
		EJC-binding motif 2, mediates interaction with the EJC. /evidence=ECO:0000250	132..152
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	178..402
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	416..468
		DUF3824	<479..552	CDD:289625
		EST1	637..740	CDD:287360	24/132 (18%)
		EST1_DNA_bind	750..1102	CDD:287359	100/608 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	820..840		6/59 (10%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	856..882		1/25 (4%)
		PIN_Smg6	1240..1415	CDD:189055	42/195 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2162
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900