DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sos and Rapgef6

DIOPT Version :9

Sequence 1:	NP_476597.2	Gene:	Sos / 34790	FlyBaseID:	FBgn0001965	Length:	1596	Species:	Drosophila melanogaster
Sequence 2:	XP_006532623.1	Gene:	Rapgef6 / 192786	MGIID:	2384761	Length:	1614	Species:	Mus musculus

Alignment Length:	1678	Identity:	319/1678 - (19%)
Similarity:	545/1678 - (32%)	Gaps:	488/1678 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   140 EAKEVI---NSKKRKSVLPTEKVHTLLQKDV-----------LQYKIDSSVSAFLVAVLEYIS-- 188
            |..|:|   |||..:.        ::||:::           :.||.:.......|.:..|:|
Mouse   111 EPSEMIVVENSKDNED--------SILQREIPARQSRRRFRKINYKGERQTIIDGVDINNYLSLP 167

  Fly   189 ADILKM--AGDYVIKIAHCEITKEDIEVVMNA-DRVLMDMLNQSEAHILPSPLS-LPAQRASATY 249
            ||:.||  ..:...::.|...::....:..:: ...|.|:...:|:.:....|: ||.....:..
Mouse   168 ADLTKMHLTDNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSED 232

  Fly   250 EETVKELIHDEKQYQ-RDLHMIIRVFREELVKIVSDPRE------LE-----PIFSNIMDIYEVT 302
            ||..:|.|......| |||      .||.|.|..:|..:      ||     |.|:|      :|
Mouse   233 EEEEEEEIDRTDPLQGRDL------VRECLEKEPADKTDDDVEQLLEFMHQLPAFAN------MT 285

  Fly   303 VTLLGSLEDVIEMSQEQSAPCVGSCFEELAEAEEFDVYKKYAYDVTSQASRDA-LNNLLSKPGAS 366
            :::...|..|:.....:.|..|     .|.:.:|.|.:..........:..|. :.||.......
Mouse   286 MSVRRELCSVMVFEVVEQAGAV-----ILEDGQELDSWYVILNGTVEISHPDGKIENLFMGNSFG 345

  Fly   367 SLTTAG----HGFRDAVKYYLPKLLLVPICHAFVYFDYIKHLKDLSSSQDDIESFEQVQGLLHPL 427
            .:.|..    ||   ||:..:.....|.|...    ||.:.|..:..:...:|.    :|.:..:
Mouse   346 IVPTLDKQHMHG---AVRTKVDDCQFVCIAQQ----DYWRILNHVEKNTHKVEE----EGEIVMV 399

  Fly   428 HCDLEKVMASLSKERQVPVSGRVRRQLAIERTRE---LQMKVEH-------WED----------- 471
            |           :.|::..||..:..:.|:.|.|   :.:..||       .||
Mouse   400 H-----------EHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLET 453

  Fly   472 -KDVGQNCNEFIREDSL-SKLGSGKRIWSERKVFLFDG--------------------------L 508
             .|||....|:.:.|:| .|:.....:|.......|:|                          |
Mouse   454 PLDVGIKLLEWFKIDNLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFERNLEDTKMNGHLRLL 518

  Fly   509 MVLCKANTK----------KQTP--------------------SAGATAYDYRLKEKYFMRRVDI 543
            .:.|.|..|          :::|                    .:|:.|.|..||....:..|:
Mouse   519 NIACAAKAKWRQVVLQKASRESPLHFCLTGGSEKGFGVFVEEVESGSKAADAGLKRGDQVMEVN- 582

  Fly   544 NDRPDSDDLKNSFELAPRMQPPIVLTAKNAQHKHDWMADLLMVITKSMLDR---------HLDSI 599
            ....::..|..:.|:. |....:.||.|..           :.:.|.:|.|         |:..|
Mouse   583 GQNFENITLAKALEIL-RNNTHLALTVKTN-----------IFVFKELLSRTEQEKSGVPHIPKI 635

  Fly   600 LQDIERKHPLRMPSPEIYKFAVPDSGDNIVLEERESAGVPMIKGATLCKLIERLTYHIY------ 658
            .:....:|.:: ..|...:.|..:.|:..:.....|.|...|:     |::::..:.|.
Mouse   636 AEKKSNRHSIQ-DVPGDMEQAPQEKGNKKIKANTVSGGRNKIR-----KILDKTRFSILPPKLFS 694

  Fly   659 -------ADPTFVRTFLTTYRYFCSPQQLLQLLVERFNIPDPSLVYQDTGTAGAGGMGGVGGDKE 716
                   .|.:.|.|     |: |.....:..:....:...|.|:...|..........||
Mouse   695 DGGLSQSQDDSIVGT-----RH-CRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSAVG---- 749

  Fly   717 HKNSHREDWKRYRKEYVQPVQFRVLNVLRHWVDHHFYDFEKDPMLLEKLLNFLEH--VNGKS--- 776
                          .|..|.|  |:.|.:......:....||....|.:...::.  :.|.|
Mouse   750 --------------FYYIPDQ--VIRVFKADQQSCYIIISKDTTAKEVVCQAVQEFGLTGASDTY 798

  Fly   777 --------------MRKWVDSVLKIVQR---------KNEQEKSNKKIVYAYGHDPPPIEHHLSV 818
                          .|:..|...|:..|         ||..|..    ......|...:     :
Mouse   799 SLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETE----TLCSDEDAQEL-----L 854

  Fly   819 PNDEITLLTLHPLELARQLTLLEFEMYKNVKPSELVGSPWTKKDKEVKSPNLLKIMKHTTNVTRW 883
            ...::::|.|..:|:|.||::.:|::::|::|:|.:...: |.|.:..:.:|.:........|.|
Mouse   855 KESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLF-KLDSKTGNTHLKQFEDIVNQETFW 918

  Fly   884 IEKSITEAENYEERLAIMQRAIEVMMVMLELNNFNGILSIVAAMGTASVYRLRWTFQGLPERYRK 948
            :...|....|..:|:.|::..|::.:...|..|||.:.:|::.:..|.|.|||.|::.||.:|.|
Mouse   919 VASEILSESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRGTWEKLPSKYEK 983

  Fly   949 FLEECRELSDD--HLKKYQERL--RSINPPCVPFFGRYLTNILHLEEGNPDLLANTELINFSKRR 1009
            .|::.::|.|.  ::.||:..|  :|:.||.:|.|.....::..|.|||...:..  |:||.|.|
Mouse   984 HLQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDG--LVNFEKLR 1046

  Fly  1010 KVAEIIGEIQQYQNQPYCLNEESTIRQFFEQLDPFNGLSDKQMSDYLYNESLRIEPRGCKTVPKF 1074
            .:|:.|..|               ||.....:||                ::....|
Mouse  1047 MIAKEIRHI---------------IRMTSANMDP----------------AMMFRQR-------- 1072

  Fly  1075 PRKWPHIPLKSPGIKPRRQNQTNSSSKLSNSTSSVAAAAAASSTATSIATASAPSLHASSIMDAP 1139
            .::|..:...|.|              .:||........|....|...:..:|..|:.    ||.
Mouse  1073 KKRWRSLGSLSQG--------------STNSNMLDVQGGAHKKRARRSSLLNAKKLYE----DAQ 1119

  Fly  1140 TAAAANAGSGTL---AGEQ-----SPQHNPHAFSVFAPVIIPERNTSSWSGTPQHTRTDQNNGEV 1196
            .|........:|   ..|:     |.|..| |:......:..:|:..|...:|...|:.....:|
Mouse  1120 MARKVKQYLSSLDIDTDEEKFQMMSLQWEP-AYGTLTKNLTEKRSAKSSEMSPVPLRSVGQTAKV 1183

  Fly  1197 SVPAPHLPKKPGAHVWANNNSTLASASAMDVVFSPALPE-------------------------H 1236
            .:..||...:.......|.:.......|.|.|.|.:||:                         |
Mouse  1184 HLHQPHRVSQVLQVPAVNLHPIRKKGQAKDHVLSTSLPQKGLGPTEEVSVKKHTEDTISVASSLH 1248

  Fly  1237 LPPQSLPDSNPFASDTEAPPSPLPKLVVSPRHETGNRSPFHGRMQNSPTHSTASTVTLTGMSTSG 1301
            ..|.:.|.::|....|..|.|....|..|...|..:||            |..|..::..||.:|
Mouse  1249 SSPPASPQNSPRKGYTLTPSSKCDNLSDSSHSEISSRS------------SIVSNGSVDSMSAAG 1301

  Fly  1302 GEEFCAGGFYFNSAHQGQP-GAVP-------ISPHVNVP--------------------MATNME 1338
            .:|.|:.    :|....:| ||:.       ||.|..:.                    .:..|.
Mouse  1302 QDERCSS----HSLAVPEPTGALEKTDHPSGISDHSQLAHGWMLSKPCLIKGVAVSSSLSSEEMS 1362

  Fly  1339 YRAVPPPLPPRRKERTESCA----DMAQKRQAP--------------DAPTLPPRDGELSPPPIP 1385
            :..|........:....||:    |..|..|..              |.|.......:..|...|
Mouse  1363 HEHVVLEAADSGRGSWTSCSSSSHDNFQSLQNQKSWDFLNSYRHMHLDDPIAEVEPTDCEPCACP 1427

  Fly  1386 PRLNHSTG-------ISYLRQSHGKSKEFVGNSSLLLPNTSSIMIRRNSAIEKRAAATSQPNQAA 1443
            ...:.:.|       .:.||||...|...   |....||..::         ||....|.|.:|.
Mouse  1428 KGCSRTCGQCKGSLETNQLRQSWASSSSL---SDTCEPNYGTV---------KRRVLESAPAEAP 1480

  Fly  1444 AG--PISTT---LVTVSQAVATD------------------EPLPLP-----ISPAASSSTTTSP 1480
            .|  |..||   ..||:.  :||                  ||.|.|     ||.|.........
Mouse  1481 DGLEPRDTTDPVYKTVTS--STDKGLIVYCVTSPKKGDRYREPPPTPPGYLGISLADLKEGPHPH 1543

  Fly  1481 LTP-------AMSPMSPN----IPSHPVESTSSSYAHQLRMRQQQQQQTHPAI 1522
            |.|       ..|.|..|    :|..|..|.:|::... ::..|.|:.:||.:
Mouse  1544 LKPPDYSVAVQRSKMMLNSLSRLPPAPPSSHTSAWVPS-KIGSQPQRHSHPKL 1595

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sos	NP_476597.2	Histone	90..216	CDD:278551	18/93 (19%)
		H2A	155..>196	CDD:305064	11/55 (20%)
		RhoGEF	249..432	CDD:238091	42/199 (21%)
		PH_SOS	476..586	CDD:269963	24/166 (14%)
		PH	482..587	CDD:278594	23/161 (14%)
		RasGEFN	637..791	CDD:214571	28/194 (14%)
		RasGEF	825..1062	CDD:238087	62/240 (26%)
Rapgef6	XP_006532623.1	CAP_ED	37..>82	CDD:381773
		CAP_ED	280..380	CDD:237999	22/117 (19%)
		RasGEFN	412..525	CDD:214571	19/112 (17%)
		PDZ_signaling	529..609	CDD:238492	12/81 (15%)
		RA_PDZ-GEF1	756..838	CDD:340483	13/83 (16%)
		RasGEF	862..1086	CDD:238087	66/279 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2063
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.940