Protein Alignment Sos and sos-1

DIOPT Version :9

• Sequence 1: NP_476597.2
• Gene: Sos / 34790
• FlyBaseID: FBgn0001965
• Length: 1596
• Species: Drosophila melanogaster
• Sequence 2: NP_001360576.1
• Gene: sos-1 / 178846
• WormBaseID: WBGene00004947
• Length: 1493
• Species: Caenorhabditis elegans
• Alignment Length: 1666
• Identity: 467/1666 - (28%)
• Similarity: 735/1666 - (44%)
• Gaps: 449/1666 - (26%)

  Fly    69 WRGLFTPSLKKVLEQVHPRV----TAKEDALLYVEKLCLRLLAMLCAKPLPHSVQDVEEKVNKSF 129
            |..:|...:.::...|||.:    .|.|....:::.:...|:.....     ||.:|::...|.|
 Worm    30 WAAIFDERIYQICNIVHPGLPIDNAAVEHIRYFLQSIVFELIEARAT-----SVVEVDKTAKKLF 89

  Fly   130 PAPIDQWALNEA-----------------KEVINSKKRKSVL------PTEKVHTLLQKDVLQYK 171
            ...: |....||                 |.|:.|:.|.:.:      |.||.    :||..:.:
 Worm    90 AFGL-QTVCKEAWDNMHQQLQKHKYQKALKTVLESQHRLAAVIKETLGPREKE----KKDREKKE 149

  Fly   172 IDSSVSAFLVAVLEYISADILKMAGDYVIKIAHCE--ITKEDIEVVMNADRVLMD----MLNQSE 230
            |: .::.::....|.::.|:|::.|:||..|.:.|  ||..:::|.||.|:.||:    :.|:.|
 Worm   150 IE-RIACYIYYACESVTEDVLRLTGNYVKNIRNSEQKITMANLDVAMNGDKALMELRTKLRNEEE 213

  Fly   231 AHILPSP-----LS----LPAQRA-------SATYEETVKELIHDEKQYQRDLHMIIRVFREELV 279
            |.   ||     ||    ..|:..       |.|||....:.:.||:::.|:|:. |.|||..:.
 Worm   214 AE---SPGGFGFLSEFEEFVAEETEEKTLSNSQTYESVAVDFLRDERRFIRELNR-INVFRRRIE 274

  Fly   280 KIVS---DPRELEPIFSNIMDIYEVTVTLLGSLEDVIEMSQEQSAPCVGSCFEELAEAEEFDVYK 341
            .:.:   |.:.:..:|.|:.:|:::.:.:..:|||.||:|..|   |:|....|..||.|||.|.
 Worm   275 SVAATDVDKQIVCNLFGNLTEIHDLALKIERTLEDAIELSDTQ---CIGMGIWEHGEAYEFDTYT 336

  Fly   342 KY------AYDVTSQAS---RDALNNLLSKPGASSL----------TTAGHGFRDAVKYYLPKLL 387
            .|      ..:.|..|:   .|.:..||.....:||          :..|..||.||:|.||:||
 Worm   337 FYIRRDGGEMNETRHATYVINDNIKALLESERFASLFQSGEHYLGSSLDGQSFRLAVQYVLPQLL 401

  Fly   388 LVPICHAFVYFDYIKHLKDLSSSQDD-------IESFEQVQGLLHPLHCDLE-KVMASLSKERQV 444
            .:||.|.:.|.:||..|..||||::|       ..:||:|.|.:..:..:|: |:...|.::
 Worm   402 HIPIFHIYQYHEYITRLHQLSSSEEDRRDLNDCRSAFERVVGCVSDMSPELKTKITQFLDQQ--- 463

  Fly   445 PVSGRVRRQLAIERTRELQMKVEHWEDKDVGQNCNEFIREDSL----------SKLGSGKRIWSE 499
               .:..:...::|..|:|..::.:....:|:.|||..::..|          |::...|:..:|
 Worm   464 ---AKSEKIYNVKRLNEIQSSIDGFTGSPIGKTCNELEKDGDLGMIRPSLQFSSEITKNKKWKTE 525

  Fly   500 RKVFLFDGLMVLCK--ANTKKQTPSAGATAYDYRLKEKYFMRRVDINDRPDSDDLKNSFELAPRM 562
            |.|::||.::||||  .||.|             .|::..:..:|:.|.||| ::.|.|::....
 Worm   526 RFVYIFDQMIVLCKRHRNTLK-------------FKDRLAVHSIDVFDIPDS-EVTNCFKIESHD 576

  Fly   563 Q---PPIV-LTAKNAQHKHDWMADLLMVITKSMLDRHLDSILQDIERKHPLRMPSPEIYKFAVPD 623
            :   |.|. ...||.:.|..|||.|:.|.|||:|||.||:..::..::.||.:|.|:.|:|:.||
 Worm   577 KSSLPKIYHFVCKNPEEKRQWMAVLVKVTTKSVLDRILDNHEKEEAKRIPLVVPGPDQYRFSEPD 641

  Fly   624 SGDNIVLEE-RESAGVPMIKGATLCKLIERLTYHIYADPTFVRTFLTTYRYFCSPQQLLQLLVER 687
            :.|||..|: ..|:|:|:||..|:.|||||||||.|.|..::.|||.:||.||:|..|..||:||
 Worm   642 TEDNISFEDYTSSSGIPVIKCGTVLKLIERLTYHSYTDSKYILTFLISYRSFCTPNDLFSLLLER 706

  Fly   688 FNIPDPSLVYQDTGTAGAGGMGGVGGDKEHKNSHRED------------WKRYRKEYVQPVQFRV 740
            ||||.|..:.|..    .|| |.:.|..:...||...            .:::|||:.||:|.||
 Worm   707 FNIPTPKKLQQPK----QGG-GPLAGRYDTVQSHGLSAISSSSCINPLCEQKFRKEFQQPIQLRV 766

  Fly   741 LNVLRHWVDHHFYDFEKDPMLLEKLLNFLEHV------NGKSMRKWVDSVLKIVQRK-------- 791
            |:|:..||..|:|||:.||:||:.|..||...      ..|..:|:..::|.:::::
 Worm   767 LSVINQWVKLHWYDFQCDPVLLDALELFLNRCCDPREGLSKQHKKFCKTILALIEKRVKNPPGIM 831

  Fly   792 ---NEQ------EKSNKKIVYAYGHDP--PPIEHHLSVPN------------------DEITLLT 827
               ||.      ::.:....:.:|.|.  || :|.:....                  |...|||
 Worm   832 QQPNENGDKGAADEGHVNSAFVFGDDQQHPP-QHQVYTNESPKETNQVLWHTAQKGDVDHYDLLT 895

  Fly   828 LHPLELARQLTLLEFEMYKNVKPSELVGSPWTKKDKEVKSPNLLKIMKHTTNVTRWIEKSITEAE 892
            |||:|:.||||||..::|:.::|.|||.:.|||.:|..|||.||::..|:|.:|.|:.:||.|.|
 Worm   896 LHPIEIGRQLTLLHSDLYRAIQPIELVEAAWTKAEKWRKSPQLLRLTDHSTLLTYWVSRSIVETE 960

  Fly   893 NYEERLAIMQRAIEVMMVMLELNNFNGILSIVAAMGTASVYRLRWTFQGLPERYRKFLEECRELS 957
            :.|||:|:..|.:|||.|..||:||.|:::..:|:.::.::||:|.:.||....:|..:....|.
 Worm   961 SLEERMAMFNRVLEVMSVFEELHNFTGLVAFYSALNSSCIFRLKWCWDGLDNEKKKCFDRFNTLC 1025

  Fly   958 DDHLKKYQERLRSINPPCVPFFGRYLTNILHLEEGN--------------------PDLLAN--- 999
            :...::.|:||.||||||:||||.||:||..||:||                    .||.|:
 Worm  1026 ERRWQEMQKRLSSINPPCIPFFGHYLSNIYFLEQGNSTFVNKSPPHGAAGAQKQQKDDLKASDPE 1090

  Fly  1000 ------TELINFSKRRKVAEIIGEIQQYQNQPYCLNEESTIRQFFEQLDPFNGL-SDKQMSDYLY 1057
                  .:|::|.|.||::.:|.|||.:|:|.|.|..|.|||||||.::|.|.. |::.:.:|||
 Worm  1091 NSNKQFKQLVSFLKLRKISNVIREIQIFQDQRYSLTLEPTIRQFFESINPKNDFKSNEDLEEYLY 1155

  Fly  1058 NESLRIEPRGCKTVPKFPRKWPH--IPLKSPGIKPRRQNQTNSSSKLSNSTSSVAAAAAASSTAT 1120
            |:||.|:|:|..| |....|..|  ..|:|||:||
 Worm  1156 NKSLEIQPKGLDT-PTAELKPKHNASTLRSPGVKP------------------------------ 1189

  Fly  1121 SIATASAPSLHASSIMDAPTAA----AANAGSGTLAGEQSPQHNPHAFSVFAPVIIPERNTSSWS 1181
                              |.||    :||...|.....|:....|||.             ||.|
 Worm  1190 ------------------PKAAGNHYSANHPIGLHLHSQNSHSAPHAM-------------SSQS 1223

  Fly  1182 GTPQHTRTDQNNGEVSVPAPHLPKKPGAHVWANNNSTLASASAMDVVFS-----PALPEHLPPQS 1241
            .|..:|..          :.|..|:..:|    |.........:|:.|.     |.:|...||..
 Worm  1224 STVPNTPL----------SAHETKRSLSH----NQDDAPLQQFVDIRFERKGTHPKIPVLQPPPL 1274

  Fly  1242 LPDSN--------PFASDTEAPPSPLPKLVVSPRHETGNRSPFHGRMQNSPTHSTASTVTLTGMS 1298
            ||.|:        .....|:||..|.||       .:|        |.::.|..|..|.|.|..|
 Worm  1275 LPRSSRANQSNSVSLPPTTQAPMPPAPK-------SSG--------MMSTATSPTTLTTTTTPSS 1324

  Fly  1299 TSGGEEFCAGGFYFNSAHQGQPGAVPISPHVNVPMATNMEYRAVPPPLPPRRKERTESCADMAQK 1363
                    |||                                .||.|.|||         |.|:
 Worm  1325 --------AGG--------------------------------PPPKLHPRR---------MTQQ 1340

  Fly  1364 RQAP--DAPTLPPRDGELSP--------------PPIPPRLNHSTGISYLRQSHGKSKEFVGNSS 1412
            ..:|  .:|..|.||.. ||              ||:|||.:.|:.:|....:.|.:      ||
 Worm  1341 PMSPLAKSPLTPSRDNS-SPSAFQFPVVYEASTAPPLPPRPSTSSDVSSSPSTSGST------SS 1398

  Fly  1413 LLLPNTSSIMIRRNSAIEKRAAATSQPNQAAAGPISTTLVTVSQAVATDEPLPLPISPAASSSTT 1477
            ....|...:.:..: ..::|...:::|:..|                          |:.|||:|
 Worm  1399 ATKENQEQLRVIFD-RFQRRITFSNRPSFCA--------------------------PSTSSSST 1436

  Fly  1478 TSPLTPAMSPMSPNIPSHPVESTSSSYAHQLRMRQQQQQQT 1518
                        ..|...|..:|:|:   :::..||||..|
 Worm  1437 ------------ERIFRVPGATTTST---KIKPSQQQQSNT 1462

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sos	NP_476597.2	Histone	90..216	CDD:278551	32/150 (21%)
		H2A	155..>196	CDD:305064	9/40 (23%)
		RhoGEF	249..432	CDD:238091	64/211 (30%)
		PH_SOS	476..586	CDD:269963	35/125 (28%)
		PH	482..587	CDD:278594	32/120 (27%)
		RasGEFN	637..791	CDD:214571	67/171 (39%)
		RasGEF	825..1062	CDD:238087	110/266 (41%)
sos-1	NP_001360576.1	RhoGEF	248..447	CDD:383033	62/202 (31%)
		PH-like	493..604	CDD:388408	35/124 (28%)
		RasGEF_N	659..794	CDD:366199	60/139 (43%)
		RasGEF	900..1069	CDD:366198	72/168 (43%)
		Neogenin_C	1148..1377	CDD:368988	81/369 (22%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C160167489
Domainoid	1	1.000	160	1.000	Domainoid score	I2478
eggNOG	1	0.900	-	-		E1_KOG3417
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4117
Inparanoid	1	1.050	553	1.000	Inparanoid score	I613
Isobase	1	0.950	-	0	Normalized mean entropy	S3092
OMA	1	1.010	-	-		QHG45786
OrthoDB	1	1.010	-	-		D262477at33208
OrthoFinder	1	1.000	-	-		FOG0002651
OrthoInspector	1	1.000	-	-		oto18146
orthoMCL	1	0.900	-	-		OOG6_101209
Panther	1	1.100	-	-	LDO	PTHR23113
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2063
SonicParanoid	1	1.000	-	-		X2024
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	18	17.750