DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sec71 and Arfgef2

DIOPT Version :9

Sequence 1:	NP_609675.2	Gene:	Sec71 / 34785	FlyBaseID:	FBgn0028538	Length:	1653	Species:	Drosophila melanogaster
Sequence 2:	NP_851597.2	Gene:	Arfgef2 / 296380	RGDID:	631430	Length:	1791	Species:	Rattus norvegicus

Alignment Length:	1811	Identity:	1077/1811 - (59%)
Similarity:	1299/1811 - (71%)	Gaps:	206/1811 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 TKTKEMFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLPK 70
            ::||.||:.||||||||||:::|..||||:::|..||::|||||       |...|..||.|
  Rat     4 SQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEL-------EKQRLGAAAPP--- 58

  Fly    71 NDAASIINAETYFLPFELACKSRSPRIVVTALDCLQKLIAYGHLTGSIQDSANPGHLLIDRIVVT 135
              .|:.|.|:.|||||||||:|:|||:|.|:||||||||||||:||:..||..||..||||||.|
  Rat    59 --KANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVET 121

  Fly   136 IYGCFSGPQTDEAVQLQIIKALLTVVTSQHVEIHEFTLLQAVRTCYDIYLSSKNLVNQTTARATL 200
            :..||.||||||.|||||||||||.|||.|:||||.|:||.|||||:|||:||||:|||||:|||
  Rat   122 VCNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATL 186

  Fly   201 TQMLNVIFARMENQVY----ELPPP---------------------------NSNPT-------- 226
            ||||||||.||||||.    ||..|                           .|.||
  Rat   187 TQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKTELP 251

  Fly   227 ---------------NGSIHSE---DCNGSGEESLRDSD---EVIASELLAEIISAAYNEAMKDQ 270
                           ||..|.|   ..:|..|.| ..||   :.:..::|.:::::|..||.:.|
  Rat   252 NGDHARSSLGKVNSENGEAHRERGSSISGRAEPS-GGSDNGAQEVVKDILEDVVTSAVKEAAEKQ 315

  Fly   271 ESVGEPEP----------------------TLNG--NDYSSHSDHDSVELHSENDAVVTAKFTHI 311
               |.|||                      ..||  :|..|.|..|::|..::... |.|:|:||
  Rat   316 ---GLPEPDQAPGVPECQECTVPPAVDENSQTNGIADDRQSLSSADNLEPDAQGHP-VAARFSHI 376

  Fly   312 LQKDAFLVFRALCKLSMKPLPDGHPDPKSHELRSKVLSLHLLLLILQNAGPVFRSNEMFIMAIKQ 376
            ||||||||||:|||||||||.:|.||||||||||||:||.|||.:||||||||||:|||:.||||
  Rat   377 LQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRSHEMFVTAIKQ 441

  Fly   377 YLCVALSNNGVSLVPEVFELSLSIFVALLSNFKVHLKRQIEVFFKEIFLNILEANSSSFEHKWMV 441
            |||||||.||||.||:||||||:||:.||||||:|||.|||||||||||||||.::|||||:|||
  Rat   442 YLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMV 506

  Fly   442 IQALTRICADAQSVVDIYVNYDCDFSAANLFERLVNDLSKIAQGRQALELGANPMQEKSMRIRGL 506
            ||.|||||||||.|||||||||||.:|||:|||||||||||||||...|||..|:||.|:|.:||
  Rat   507 IQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGL 571

  Fly   507 ECLVSILKCMVEWSKDLYVNPNMPVPPMQVQSPTSTEQDQAD------------TTIQ-TMHSGS 558
            ||||||||||||||||||||||......|.:.|   :|:..|            |::: |:.||:
  Rat   572 ECLVSILKCMVEWSKDLYVNPNHQATLGQERLP---DQEMGDGKGLDMARRCSVTSVESTVSSGT 633

  Fly   559 SHSLNSNQEQLQDLPEALEERKMRKEVMETGIELFNRKPQKGVQFLQEKQLLGATCGDIARWLHE 623
                   |..:.|.||..|..|.:||::|.||||||:||::|:|||||:.:|||...|||::||:
  Rat   634 -------QTAIPDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQ 691

  Fly   624 DERLDKTVIGNYIGENDDHSKEVMCAYIDAFDFRQMEVVAALRFLLEGFRLPGEAQKIDRLMEKF 688
            :||||.|.:|.::|::...:||||.||:|..||.:.|.|:|||..||||||||||||||||||||
  Rat   692 EERLDSTQVGEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKF 756

  Fly   689 ASRYCECNPKNQLFQSADTVYVLAFSIIMLTTDLHSPQVKHKMTKEQYIKMNRGISDSKSDLPEE 753
            |:||.|||....||.||||.||||:|||||||||||||||:||||||||||||||:||| |||||
  Rat   757 AARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSK-DLPEE 820

  Fly   754 YLSSIYDEISEHEIKMKNNSGMLQQAKPTGKQAFITEKRRKLLWNMEMEVISLTATNLMQSVSHV 818
            ||||||:||...:|.||.........|.| ||...:||:|:||:|:|||.::.||..||::|||.
  Rat   821 YLSSIYEEIEGKKIAMKETKEHTMATKST-KQNVASEKQRRLLYNVEMEQMAKTAKALMEAVSHA 884

  Fly   819 KSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPEIATLCLDGIRCAIRIACIFHMSLERD 883
            |:|||||.||:|||||||:.|||.|||:|:|||:|||.|:|:|||:|||||:||||||.|.||||
  Rat   885 KAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERD 949

  Fly   884 AYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCISQLELAQLIGT 948
            |||||||||:||.|:|.|.|||.||||||||||.||||||||||:||.:|:||||||||||||||
  Rat   950 AYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGT 1014

  Fly   949 GVRPQFLSGAQTTLKDSLN---------------------------PSVKEHIGETSSQSVVVAV 986
            ||:.::|||:....:.||.                           .|.:|.:||||||||||||
  Rat  1015 GVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNLVSGGVDKRQMASFQESVGETSSQSVVVAV 1079

  Fly   987 DRIFTGSMRLDGDAIVDFVKALCQVSVDEL-QQQQPRMFSLQKIVEISYYNMERIRLQWSRIWQV 1050
            |||||||.||||:||||||:.||.||:||| ....|||||||||||||||||.||||||||||.|
  Rat  1080 DRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHV 1144

  Fly  1051 LGEHFNAVGCNSNEEISFFALDSLRQLSMKFMEKGEFSNFRFQKDFLRPFEHIMKKNASPAIRDM 1115
            :|:|||.||||.||:::.||:||||||||||:||||.:|||||||||||||||||||.||.||||
  Rat  1145 IGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDM 1209

  Fly  1116 VVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDNEEPIVELAFQTTGKIIGDLYKRQFAIMVDSF 1180
            |:|||||||:|||.||||||||||::||.||.|::..||||||||||.|:..:::..|...:|||
  Rat  1210 VIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVSTIFQHHFPAAIDSF 1274

  Fly  1181 QDAVKCLSEFA-TARFPDTSMEAIRLVRTCAQCVHEAPQLFAEHAGMENDASVAEEDRVWVRGWF 1244
            ||||||||||| .|.|||||||||||:|.|.:.|.|.|::..|:.  .:|.:||..|||||||||
  Rat  1275 QDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYT--SDDMNVAPGDRVWVRGWF 1337

  Fly  1245 PMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPHWWKDLFNVIFRIFDNMKLPEHVTEKS 1309
            |:||.|||::||||||||||.|||:|||:|:||.:|..|||:|||.::|||||||||||..:|||
  Rat  1338 PILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSEKS 1402

  Fly  1310 EWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCVQQSNEQLARSGTNCLENLVISNGF 1374
            ||||||||||||||.|||||:::.|..:||.::||||.|||:|.||||||||||||||||||||.
  Rat  1403 EWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGE 1467

  Fly  1375 KFNESTWDKTCQCILDIFNATLPQDLLSWRPKAHSSNNIPQEH---------------NHFE--- 1421
            ||:.:.||:||.|:||||..|:|..||:||| |.....:...|               |..|
  Rat  1468 KFSPAVWDETCNCMLDIFRTTIPHVLLTWRP-AGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQ 1531

  Fly  1422 -----------------------ALHIRCVVQLELIQTMDNIVFFPATSRKEDAETLAQAAADLT 1463
                                   :|.|:||||||||||:|||||:||||:|||||.:..|..|..
  Rat  1532 SQLSNPTDDSWKGAPYANQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTL 1596

  Fly  1464 GGRSGSQSQLLECQREEQGMYGYLRTRQLLTLADCLMQSHRFAKRFNADHDQRSLLWRAGFKGSV 1528
            ...       :..:.|.||||.::.::.|..|.|||.:||.|:|.||::::||::||||||||..
  Rat  1597 DAD-------IHIETENQGMYKFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKS 1654

  Fly  1529 KPNLLKQETSSLACVLRIFFKMYGDENRRSDWPGIEQELVQVCKEALGYYLSLQSEAHRDAWTSL 1593
            ||||||||||||||.|||.|:||.|||||..|..|:|.|:.||.|||.|::::.||:||:|||||
  Rat  1655 KPNLLKQETSSLACCLRILFRMYVDENRRDSWGEIQQRLLTVCSEALAYFITVNSESHREAWTSL 1719

  Fly  1594 LLLILTRLLKMSDARFATHVSNYYSLLCEMMCFDLKPELRSVLRRVFMRIGPVFNI 1649
            |||:||:.||::|.:|..|.|.||..|||||.|||.||||:|||:.|:|||.|:.|
  Rat  1720 LLLLLTKTLKINDEKFKAHASVYYPYLCEMMQFDLIPELRAVLRKFFLRIGLVYKI 1775

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sec71	NP_609675.2	PLN03076	13..1580	CDD:215560	1030/1733 (59%)
		DCB	30..212	CDD:292830	119/181 (66%)
		Sec7_N	312..465	CDD:289549	127/152 (84%)
		Sec7	582..767	CDD:279680	127/184 (69%)
		DUF1981	1080..1161	CDD:286414	64/80 (80%)
Arfgef2	NP_851597.2	DCB, DCB:DCB domain and DCB:HUS domain interaction. /evidence=ECO:0000250	2..224		142/231 (61%)
		PLN03076	11..1718	CDD:215560	1036/1745 (59%)
		DCB	28..198	CDD:292830	119/181 (66%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	208..292		14/84 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	311..350		8/41 (20%)
		Sec7_N	377..531	CDD:289549	128/153 (84%)
		HUS, DCB:HUS domain interaction. /evidence=ECO:0000250	515..535		16/19 (84%)
		Sec7	650..834	CDD:279680	127/184 (69%)
		DUF1981	1174..1255	CDD:286414	64/80 (80%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166342761
Domainoid	1	1.000	613	1.000	Domainoid score	I179
eggNOG	1	0.900	-	-		E1_COG5307
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2090	1.000	Inparanoid score	I39
OMA	1	1.010	-	-		QHG54931
OrthoDB	1	1.010	-	-		D50759at33208
OrthoFinder	1	1.000	-	-		FOG0001583
OrthoInspector	1	1.000	-	-		otm45422
orthoMCL	1	0.900	-	-		OOG6_100729
Panther	1	1.100	-	-	LDO	PTHR10663
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X714
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.770