DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sec71 and arfgef2

DIOPT Version :9

Sequence 1:	NP_609675.2	Gene:	Sec71 / 34785	FlyBaseID:	FBgn0028538	Length:	1653	Species:	Drosophila melanogaster
Sequence 2:	XP_002666513.2	Gene:	arfgef2 / 100334572	ZFINID:	ZDB-GENE-090312-50	Length:	1846	Species:	Danio rerio

Alignment Length:	1839	Identity:	1084/1839 - (58%)
Similarity:	1293/1839 - (70%)	Gaps:	221/1839 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     5 STKTKEMFIVRALEKILADKDIRRSHHSQLKKSCDSALEQIKAELISAGQIAEGNELPCAALPLP 69
            |..||.||:.||||||||||:::||.|:||:|:|..||::||.||   .:..:|..:|      |
Zfish    18 SLSTKSMFLSRALEKILADKEVKRSQHNQLRKACQVALDEIKHEL---EKQKDGTVVP------P 73

  Fly    70 KNDAASIINAETYFLPFELACKSRSPRIVVTALDCLQKLIAYGHLTGSIQDSANPGHLLIDRIVV 134
            :   |:.|.|:.|.|||||||:|:|||||.|:||||||||||||:||:..|||.||..||||:|.
Zfish    74 R---ANYIEADKYVLPFELACQSKSPRIVSTSLDCLQKLIAYGHITGNAPDSAAPGKRLIDRLVE 135

  Fly   135 TIYGCFSGPQTDEAVQLQIIKALLTVVTSQHVEIHEFTLLQAVRTCYDIYLSSKNLVNQTTARAT 199
            ||..||.||||||.|||||||||||.|||.|:||||.|:|..|||||:|||:|:||:|||||:||
Zfish   136 TICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILLTVRTCYNIYLASRNLINQTTAKAT 200

  Fly   200 LTQMLNVIFARMENQ----------------------VYELPPP-NSNPTN-----------GSI 230
            |||||||||.|||||                      |...|.| |.||:.           |..
Zfish   201 LTQMLNVIFTRMENQAALEAQEAEKERQRLQLRNPSPVSSNPSPVNQNPSQTPGRGSGSPWMGRA 265

  Fly   231 HSEDCNGS--------------------------------------------------------- 238
            .||. |||
Zfish   266 CSEQ-NGSPCLSPPGVPSTPSTPQDSSSINSRPEEEFATDKQSTKDITHPDEELQESDQTTVDPS 329

  Fly   239 ------GEESLRDSDEVIASELLAEI-----ISAAYNEAMKDQESVGE---PEPT-------LNG 282
                  |||:...|:....||:|.|.     :..:..|...||.|..|   |.|.       :||
Zfish   330 VTGGVEGEETHPQSETEAGSEVLEETEPDSGLGDSSVEGGLDQHSDSETRMPPPVVRVDTQQMNG 394

  Fly   283 --NDYSSHSDHDSVELHSENDAVVTAKFTHILQKDAFLVFRALCKLSMKPLPDGHPDPKSHELRS 345
              :|..|.|..|.::..|.:.:...|:|:||||||||||||:|||||||||.||.||||||||||
Zfish   395 MVDDRGSESSTDMLDAESIHGSQTAARFSHILQKDAFLVFRSLCKLSMKPLADGPPDPKSHELRS 459

  Fly   346 KVLSLHLLLLILQNAGPVFRSNEMFIMAIKQYLCVALSNNGVSLVPEVFELSLSIFVALLSNFKV 410
            ||:||.|||.:||.||||||::|||:.|||||||||||.||||.|||||||||:||:.|||:|||
Zfish   460 KVVSLQLLLSVLQGAGPVFRTHEMFVNAIKQYLCVALSKNGVSSVPEVFELSLAIFLTLLSHFKV 524

  Fly   411 HLKRQIEVFFKEIFLNILEANSSSFEHKWMVIQALTRICADAQSVVDIYVNYDCDFSAANLFERL 475
            |||.||||||:||||.|||.:||||||||||||.|||||||||.|||||||||||.:|||:||||
Zfish   525 HLKMQIEVFFREIFLTILETSSSSFEHKWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERL 589

  Fly   476 VNDLSKIAQGRQALELGANPMQEKSMRIRGLECLVSILKCMVEWSKDLYVNPNMPVPPMQVQSPT 540
            |||||||||||...|||..|:||.|:|.:||||||||||||||||:|:|||||:..   .:....
Zfish   590 VNDLSKIAQGRSGQELGMTPLQELSLRKKGLECLVSILKCMVEWSRDMYVNPNLQA---NLGQER 651

  Fly   541 STEQDQADTTIQ---TMHSGSSHSLNSN-----QEQLQDLPEALEERKMRKEVMETGIELFNRKP 597
            ..|.|.||..:.   :....|..||:|.     |:...|.||..|..|.:||::|.||||||:||
Zfish   652 PAEGDSADVKLSEHLSSRRDSVSSLDSTVSSGVQQSQPDHPEQYEVIKQQKEIIEHGIELFNKKP 716

  Fly   598 QKGVQFLQEKQLLGATCGDIARWLHEDERLDKTVIGNYIGENDDHSKEVMCAYIDAFDFRQMEVV 662
            ::|:|:|||:.:||.:..|||::|.::||||.|.:|.::|||...:||||..|:|..||...:.|
Zfish   717 KRGLQYLQEQGMLGTSPEDIAQFLQQEERLDTTQVGEFLGENVKFNKEVMYCYVDRLDFCGKDFV 781

  Fly   663 AALRFLLEGFRLPGEAQKIDRLMEKFASRYCECNPKNQLFQSADTVYVLAFSIIMLTTDLHSPQV 727
            :|||..|||||||||||||||||||||:||.|||....||.||||.||||:||||||||||||||
Zfish   782 SALRAFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQV 846

  Fly   728 KHKMTKEQYIKMNRGISDSKSDLPEEYLSSIYDEISEHEIKMKNNSGMLQQAKPTGKQAFITEKR 792
            |:||||||||||||||:||| ||||||||||||||:..:|.||.:.......| :.||....||:
Zfish   847 KNKMTKEQYIKMNRGINDSK-DLPEEYLSSIYDEIAGKKIAMKESKEYSITPK-SSKQNVANEKQ 909

  Fly   793 RKLLWNMEMEVISLTATNLMQSVSHVKSPFTSAKHLEHVRPMFKMAWTPFLAAFSVGLQDCDDPE 857
            |:||:|||||.::.||..||::|||.::||.||.||||||||||:||||.|||||||||||||.:
Zfish   910 RRLLYNMEMEQMAKTAKALMEAVSHAQAPFFSATHLEHVRPMFKLAWTPLLAAFSVGLQDCDDQD 974

  Fly   858 IATLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINEMKAKNIDTIKTLIMVAHTD 922
            :|:|||:||||||||||||:|.||||||:|||||||||.|:|.|.|||.||||||||||||||||
Zfish   975 VASLCLEGIRCAIRIACIFNMQLERDAYIQALARFTLLTASSSITEMKQKNIDTIKTLIMVAHTD 1039

  Fly   923 GNYLGSSWLDIVKCISQLELAQLIGTGVRPQ--------------FLSGAQTTLKDSLN------ 967
            |||||:||.:|::|||||||||||||||:.:              |.||.:..:...|.
Zfish  1040 GNYLGNSWHEILRCISQLELAQLIGTGVKMRISGVVRDQGGGIKGFPSGGEEFMPLGLGTLVGGP 1104

  Fly   968 -----PSVKEHIGETSSQSVVVAVDRIFTGSMRLDGDAIVDFVKALCQVSVDEL-QQQQPRMFSL 1026
                 ..::|.:|||||||||||||||||||.||||:||||||:.||.||:||| ...|||||||
Zfish  1105 DKRQMAHIQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASAHQPRMFSL 1169

  Fly  1027 QKIVEISYYNMERIRLQWSRIWQVLGEHFNAVGCNSNEEISFFALDSLRQLSMKFMEKGEFSNFR 1091
            |||||||||||.||||||||||||:|:|||.||||.||:::.||:||||||||||:||||.:|||
Zfish  1170 QKIVEISYYNMNRIRLQWSRIWQVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFR 1234

  Fly  1092 FQKDFLRPFEHIMKKNASPAIRDMVVRCIAQMVNSQAHNIRSGWKNIFSIFHLAAGDNEEPIVEL 1156
            ||||||||||||:|||.||.|||||:||:||||||||.|||||||||||:||.||.|::|.||:|
Zfish  1235 FQKDFLRPFEHIVKKNRSPTIRDMVIRCVAQMVNSQAANIRSGWKNIFSVFHQAASDHDENIVDL 1299

  Fly  1157 AFQTTGKIIGDLYKRQFAIMVDSFQDAVKCLSEF-ATARFPDTSMEAIRLVRTCAQCVHEAPQLF 1220
            ||||||.|:.:.:::.||..:||||||||||||| ..|.|||||||||||:|.||:.|.|.||..
Zfish  1300 AFQTTGHIVMNTFQQHFAAAIDSFQDAVKCLSEFVCNAAFPDTSMEAIRLIRHCAKYVSERPQAL 1364

  Fly  1221 AEHAGMENDASVAEEDRVWVRGWFPMLFSLSCVVNRCKLDVRTRALTVLFEIVKTYGESFKPHWW 1285
            .|:.  .:|.:||..||||||||||:||.|||::||||||||||.|||:|||:|:||.:|:.|||
Zfish  1365 REYT--SDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWW 1427

  Fly  1286 KDLFNVIFRIFDNMKLPEHVTEKSEWMTTTCNHALYAIIDVFTQYFDVLGHLLLEELFAQLHWCV 1350
            .|||.:||||||||||||..|||:||||||||||||||.|||||:::.|..:||.::||||.|||
Zfish  1428 HDLFRIIFRIFDNMKLPEQQTEKTEWMTTTCNHALYAICDVFTQFYEPLSEVLLADIFAQLQWCV 1492

  Fly  1351 QQSNEQLARSGTNCLENLVISNGFKFNESTWDKTCQCILDIFNATLPQDLLSWRP---------- 1405
            :|.|||||||||||||||||.||.||:...||.||.|:||||..|.|..||:|||
Zfish  1493 KQDNEQLARSGTNCLENLVILNGEKFSPEVWDVTCSCMLDIFQTTSPHALLTWRPAGQEEEVGEG 1557

  Fly  1406 ---------------------KAHS-------------SNNIPQEHNHFEALHIRCVVQLELIQT 1436
                                 :.||             |:....:|..|..|.|:||||||||||
Zfish  1558 KHMDVEVDSQSQSSFERTLSERGHSQMSTASDETWKGKSHTRVSDHRLFAGLLIKCVVQLELIQT 1622

  Fly  1437 MDNIVFFPATSRKEDAETLAQAAADLTGGRSGSQSQLLECQRE-EQGMYGYLRTRQLLTLADCLM 1500
            :|||||:||||::||||.:|.|..|       :..|..|.:.| :||||.:|.::.|..|.|||:
Zfish  1623 IDNIVFYPATSKREDAENMAAAQRD-------ALEQAEEGEAEVDQGMYRHLSSQHLFKLLDCLL 1680

  Fly  1501 QSHRFAKRFNADHDQRSLLWRAGFKGSVKPNLLKQETSSLACVLRIFFKMYGDENRRSDWPGIEQ 1565
            :||||||.||::::||:.||||||||..||||||||||||||.|||.|:||.|...|..||.|:.
Zfish  1681 ESHRFAKDFNSNNEQRTALWRAGFKGKSKPNLLKQETSSLACSLRILFRMYSDTQLRDSWPDIQT 1745

  Fly  1566 ELVQVCKEALGYYLSLQSEAHRDAWTSLLLLILTRLLKMSDARFATHVSNYYSLLCEMMCFDLKP 1630
            .|:.||.|||.|::||.||:||:||||||||:|||.|::.|.:|..|.|.||..|||||.|||.|
Zfish  1746 RLLHVCSEALAYFISLTSESHREAWTSLLLLLLTRTLRLPDDKFKPHASCYYHHLCEMMQFDLIP 1810

  Fly  1631 ELRSVLRRVFMRIGPVFNI 1649
            |||:||||.|:|||.||:|
Zfish  1811 ELRAVLRRFFLRIGSVFHI 1829

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sec71	NP_609675.2	PLN03076	13..1580	CDD:215560	1032/1760 (59%)
		DCB	30..212	CDD:292830	115/181 (64%)
		Sec7_N	312..465	CDD:289549	127/152 (84%)
		Sec7	582..767	CDD:279680	125/184 (68%)
		DUF1981	1080..1161	CDD:286414	64/80 (80%)
arfgef2	XP_002666513.2	PLN03076	16..1760	CDD:215560	1037/1768 (59%)
		DCB	38..213	CDD:292830	117/186 (63%)
		Sec7_N	426..580	CDD:289549	128/153 (84%)
		Sec7	701..885	CDD:279680	125/184 (68%)
		DUF1981	1223..1304	CDD:286414	64/80 (80%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170582833
Domainoid	1	1.000	612	1.000	Domainoid score	I183
eggNOG	1	0.900	-	-		E1_COG5307
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	2089	1.000	Inparanoid score	I41
OMA	1	1.010	-	-		QHG54931
OrthoDB	1	1.010	-	-		D50759at33208
OrthoFinder	1	1.000	-	-		FOG0001583
OrthoInspector	1	1.000	-	-		otm26363
orthoMCL	1	0.900	-	-		OOG6_100729
Panther	1	1.100	-	-	LDO	PTHR10663
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1314
SonicParanoid	1	1.000	-	-		X714
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800