DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sirt1 and Sirt1

DIOPT Version :9

Sequence 1:	NP_477351.1	Gene:	Sirt1 / 34708	FlyBaseID:	FBgn0024291	Length:	823	Species:	Drosophila melanogaster
Sequence 2:	NP_062786.1	Gene:	Sirt1 / 93759	MGIID:	2135607	Length:	737	Species:	Mus musculus

Alignment Length:	752	Identity:	296/752 - (39%)
Similarity:	395/752 - (52%)	Gaps:	156/752 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly    48 AEAEAEATATTTEPAT--SELAG-KANGEIKTKTLAAREEQEIGANLEHKTKNPTKSMGEDEDDE 109
            |.|..|| |:...||.  .|.|| .|:.|.:....|...:.:.|:.|..:.:     ..:|.||:
Mouse    57 AAAGCEA-ASAAAPAALWREAAGAAASAEREAPATAVAGDGDNGSGLRREPR-----AADDFDDD 115

  Fly   110 EEEEEDDEEEE--------EDD--------EEGITGTSNEDEDSSSNCSSSVEPDWKLR------ 152
            |.||||:....        .|:        ..|.....::|:|.:|:.|||   ||..|
Mouse   116 EGEEEDEAAAAAAAAAIGYRDNLLLTDGLLTNGFHSCESDDDDRTSHASSS---DWTPRPRIGPY 177

  Fly   153 -WLQREFYTGRVPRQVIASIMPHFATGLAGDTDDSVLWDYLAHLLNEPKRRNKLASVNTFDDVIS 216
             ::|:....|..||.::..::|.  |....:.||..||..:.::|:||.:|.|...:||.:|.:.
Mouse   178 TFVQQHLMIGTDPRTILKDLLPE--TIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVK 240

  Fly   217 LVKKSQKIIVLTGAGVSVSCGIPDFRSTNGIYARLAHDFPDLPDPQAMFDINYFKRDPRPFYKFA 281
            |:::.:|||||||||||||||||||||.:|||||||.||||||||||||||.||::|||||:|||
Mouse   241 LLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFA 305

  Fly   282 REIYPGEFQPSPCHRFIKMLETKGKLLRNYTQNIDTLERVAGIQRVIECHGSFSTASCTKCRFKC 346
            :|||||:||||.||:||.:.:.:|||||||||||||||:||||||:::|||||:||||..|::|.
Mouse   306 KEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRILQCHGSFATASCLICKYKV 370

  Fly   347 NADALRADIFAQRIPVCPQCQPNKEQSVDASVAVTEEELRQLVENGIMKPDIVFFGEGLPDEYHT 411
            :.:|:|.|||.|.:|.||:|.             .:|.|      .||||:||||||.||:::|.
Mouse   371 DCEAVRGDIFNQVVPRCPRCP-------------ADEPL------AIMKPEIVFFGENLPEQFHR 416

  Fly   412 VMATDKDVCDLLIVIGSSLKVRPVAHIPSSIPATVPQILINREQLHHLKFDVELLGDSDVIINQI 476
            .|..|||..||||||||||||||||.||||||..|||||||||.|.||.||||||||.|||||::
Mouse   417 AMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINEL 481

  Fly   477 CHRLSDNDDCWRQLCCDESVLTESKELMP-PEHSNHHLHH---HLLHHRHCSSESERQSQLDTDT 537
            ||||...   :.:|||:...|:|..|..| |:....||..   ..||....||..||
Mouse   482 CHRLGGE---YAKLCCNPVKLSEITEKPPRPQKELVHLSELPPTPLHISEDSSSPER-------- 535

  Fly   538 QSIKSNSSADYILGSAGTCSD-SGFESSTFSCGKRSTAAEAAAIERIKTDILVELNETTALSCDR 601
             ::..:||....|....|.:: :..|.|..||                    ||
Mouse   536 -TVPQDSSVIATLVDQATNNNVNDLEVSESSC--------------------VE----------- 568

  Fly   602 LGLEGPQTTVESYRHLSIDSSKDSGIEQCDNEATPSYVRPSNLVQETKTVAPSLTPIPQQRGKRQ 666
               |.|| .|::.|::     ::..:|..|.:|..|.....|   |..:||.::......|..::
Mouse   569 ---EKPQ-EVQTSRNV-----ENINVENPDFKAVGSSTADKN---ERTSVAETVRKCWPNRLAKE 621

  Fly   667 TAAERLQPGTFYSHTNNYSYVFPGAQVFWDNDYSDDDDEEEERSHNRHSDLFGNVGHNYKDDDED 731
            ..::||: |..|.......|:|.||:|     |||.:|:....|         :.|.|   .|..
Mouse   622 QISKRLE-GNQYLFVPPNRYIFHGAEV-----YSDSEDDVLSSS---------SCGSN---SDSG 668

  Fly   732 ACDLNAVPLSPLLPPSLEAHIVTDIVNGSNEPLPNSS 768
            .|.          .||||            |||.:.|
Mouse   669 TCQ----------SPSLE------------EPLEDES 683

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sirt1	NP_477351.1	DUF592	<202..228	CDD:282439	10/25 (40%)
Sirt1	NP_477351.1	SIRT1	222..476	CDD:238699	173/253 (68%)
Sirt1	NP_062786.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..56
		Interaction with H1-4. /evidence=ECO:0000250\|UniProtKB:Q96EB6	2..268		85/236 (36%)
		Interaction with CLOCK. /evidence=ECO:0000269\|PubMed:18662547	2..131		23/84 (27%)
		Nuclear localization signal	32..39
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	75..125		14/50 (28%)
		Interaction with CCAR2. /evidence=ECO:0000250\|UniProtKB:Q96EB6	135..533		218/408 (53%)
		Nuclear export signal	138..145		1/6 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	152..171		6/25 (24%)
		Nuclear localization signal	223..230		1/6 (17%)
		SIRT1	246..481	CDD:238699	155/234 (66%)
		Required for interaction with the sumoylated form of CCAR2. /evidence=ECO:0000250\|UniProtKB:Q96EB6	248..251		2/2 (100%)
		Nuclear export signal	425..431		3/5 (60%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	514..539		9/28 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	653..713		16/59 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850551
Domainoid	1	1.000	278	1.000	Domainoid score	I1705
eggNOG	1	0.900	-	-		E1_COG0846
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	423	1.000	Inparanoid score	I1760
Isobase	1	0.950	-	0	Normalized mean entropy	S1880
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D512963at33208
OrthoFinder	1	1.000	-	-		FOG0000643
OrthoInspector	1	1.000	-	-		oto93419
orthoMCL	1	0.900	-	-		OOG6_103854
Panther	1	1.100	-	-	LDO	PTHR48252
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R577
SonicParanoid	1	1.000	-	-		X3771
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.740