Protein Alignment CG5776 and SPAST

DIOPT Version :10

• Sequence 1: NP_609585.1
• Gene: CG5776 / 34680
• FlyBaseID: FBgn0032450
• Length: 799
• Species: Drosophila melanogaster
• Sequence 2: NP_055761.2
• Gene: SPAST / 6683
• HGNCID: 11233
• Length: 616
• Species: Homo sapiens
• Alignment Length: 628
• Identity: 155/628 - (24%)
• Similarity: 266/628 - (42%)
• Gaps: 171/628 - (27%)

  Fly   196 RVFYKDSLIHMNFFNKSLTFRL-------------ERWQSTVEEALAGLSLDSKPLQFIQVTNVT 247
            |||:|.:..::     |:..|:             |.::..:||...|:::
Human   117 RVFHKQAFEYI-----SIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAV-------------- 162

  Fly   248 KLHLITDDANQQEEEQKISHRITKCQIGGLDRQLQLVEESMEYALGFRTLPAGLRVSRGLLLYGA 312
               ::|....|.|..:::..::....:...|| |||:|: |:..|.|
Human   163 ---IVTGQGEQCERARRLQAKMMTNLVMAKDR-LQLLEK-MQPVLPF------------------ 204

  Fly   313 TGCGKSMVLEAMCAVAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLI 377
                             .:||..|    .|.....:...|..:.:.||:.:|     ..|    :
Human   205 -----------------SKSQTDV----YNDSTNLACRNGHLQSESGAVPKR-----KDP----L 239

  Fly   378 EDVHNLCPKQENSDLVKRVSLAFLS---LLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGR 439
            ....|..|:   |..|.:...|.||   .....|..|.:.|.|    ..|....|.|....:..|
Human   240 THTSNSLPR---SKTVMKTGSAGLSGHHRAPSYSGLSMVSGVK----QGSGPAPTTHKGTPKTNR 297

  Fly   440 LDNEVELGAPSSQARLEIVRCLIKSVEHQLSDEEVEHVASITHGYVGADLANLVYAAMLQAQPNP 504
            .:..   ..|::..|               ..:::::..:     |.::||||:
Human   298 TNKP---STPTTATR---------------KKKDLKNFRN-----VDSNLANLI----------- 328

  Fly   505 LQMPHLQAALTRIKPSAMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADKFQRLGIK-P 568
                             |.|::.....|::.||.||...:.|:|:.:..|.|..:.|  .|:: |
Human   329 -----------------MNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELF--TGLRAP 374

  Fly   569 PRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKARQVAPAIVF 633
            .||:|:|||||..|||:|||:|.||...|.:|....|.|.:|||.|:.||.:|..||::.|:|:|
Human   375 ARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIF 439

  Fly   634 FDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEAL--QNVTIVAATNRPDMIDKALLRP 696
            .||:|::..||.||:..:|     .|:.|:.|.|.|||::.  ..|.::.|||||..:|:|:||
Human   440 IDEVDSLLCERREGEHDAS-----RRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLR- 498

  Fly   697 GRIDRILYVGLPQCEARREILKIKL--RAMPISNDVDMEKLVQLTEGYSGAEIQAVCHEAALRAL 759
             |..:.:||.||..|.|..:||..|  :..|::.. ::.:|.::|:||||:::.|:..:|||..:
Human   499 -RFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQK-ELAQLARMTDGYSGSDLTALAKDAALGPI 561

  Fly   760 EQ----------SFEAEDVKWTDFEHALKAVPPRTSPELLKLY 792
            .:          :.|..:::.:||..:||.:....||:.|:.|
Human   562 RELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAY 604

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG5776	NP_609585.1	CDC48	183..797	CDD:273521	155/628 (25%)
SPAST	NP_055761.2	Required for interaction with RTN1. /evidence=ECO:0000269|PubMed:16602018	1..300		46/261 (18%)
		Required for midbody localization. /evidence=ECO:0000269|PubMed:18997780	1..194		16/99 (16%)
		Required for interaction with ATL1. /evidence=ECO:0000269|PubMed:16339213, ECO:0000269|PubMed:16815977	1..80
		Required for nuclear localization. /evidence=ECO:0000269|PubMed:15147984	1..50
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..43
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000269|PubMed:15269182	50..87
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:16026783	59..67
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000269|PubMed:18997780	112..196		18/101 (18%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:15269182	114..200		20/106 (19%)
		MIT_spastin	116..195	CDD:239142	17/100 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	224..266		11/53 (21%)
		Sufficient for microtubule severing. /evidence=ECO:0000269|PubMed:15269182	228..616		128/454 (28%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000269|PubMed:15269182	270..328		14/84 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	278..312		7/55 (13%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021, ECO:0000269|PubMed:15147984	309..312		1/17 (6%)
		Required for interaction with microtubules. /evidence=ECO:0000269|PubMed:23272056	310..312		0/1 (0%)
		RecA-like_spastin	343..506	CDD:410932	70/171 (41%)
		AAA_lid_3	533..587	CDD:465537	13/53 (25%)
		Vps4_C	<580..612	CDD:462762	8/25 (32%)