Protein Alignment CG5776 and Spast

DIOPT Version :10

• Sequence 1: NP_609585.1
• Gene: CG5776 / 34680
• FlyBaseID: FBgn0032450
• Length: 799
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 507
• Identity: 144/507 - (28%)
• Similarity: 235/507 - (46%)
• Gaps: 76/507 - (14%)

  Fly   327 VAEERSQGHVQLIRINSGEVYSKFLGETEQKLGAIFERAYNHYPHPTLLLIEDVHNLCPKQENSD 391
            :.||...|..:    .:.|.|.|.:.|.|:.:..|.......|.....|..:.:.||...::.
Mouse   131 IDEEEKAGQKE----QAVEWYKKGIEELEKGIAVIVTGQGEQYERARRLQAKMMTNLVMAKDR-- 189

  Fly   392 LVKRVSLAFLSLLDQLSSPSQLKGSKTFVLATSSQIDTLHPSIRRAGRLDNEVELGA------PS 450
                     |.||::|....|...|:|.|...|:.:..      |.|.|.:  |.||      |.
Mouse   190 ---------LQLLEKLQPVLQFSKSQTDVYNESTNLTC------RNGHLQS--ESGAVPKRKDPL 237

  Fly   451 SQARLEIVR--CLIKSVEHQLSDE----EVEHVASITHGYVGADLANLVYAAMLQAQPNPLQMPH 509
            :.|...:.|  .::||....||..    ....::.::....|...|...:..  ..:||....|.
Mouse   238 THASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKG--TPKPNRTNKPS 300

  Fly   510 LQAALTRIKPS--------------AMREVLIECPNVQWSDIGGQSELRLAMQQAIEWPLLHADK 560
            ......|.|..              .|.|::.....|::.||.||...:.|:|:.:..|.|..:.
Mouse   301 TPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVILPSLRPEL 365

  Fly   561 FQRLGIK-PPRGILMFGPPGCSKTMIAKALATESKLNFLSIKGPELFSMWVGESERAVREVFRKA 624
            |  .|:: |.||:|:|||||..|||:|||:|.||...|.:|....|.|.:|||.|:.||.:|..|
Mouse   366 F--TGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVA 428

  Fly   625 RQVAPAIVFFDEIDAIGGERSEGDGSSSGSSVKERVLTQLLTELDGVEAL--QNVTIVAATNRPD 687
            |::.|:|:|.||:|::..||.||:..:|     .|:.|:.|.|.|||::.  ..|.::.|||||.
Mouse   429 RELQPSIIFIDEVDSLLCERREGEHDAS-----RRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQ 488

  Fly   688 MIDKALLRPGRIDRILYVGLPQCEARREILKIKL--RAMPISNDVDMEKLVQLTEGYSGAEIQAV 750
            .:|:|:||  |..:.:||.||..|.|..:||..|  :..|::.. ::.:|.::|:||||:::.|:
Mouse   489 ELDEAVLR--RFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQK-ELAQLARMTDGYSGSDLTAL 550

  Fly   751 CHEAALRALEQ----------SFEAEDVKWTDFEHALKAVPPRTSPELLKLY 792
            ..:|||..:.:          :.|..:::.:||..:||.:....||:.|:.|
Mouse   551 AKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAY 602

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CG5776	NP_609585.1	CDC48	183..797	CDD:273521	144/507 (28%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298		39/191 (20%)
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192		14/75 (19%)
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194		15/77 (19%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198		17/81 (21%)
		MIT_spastin	114..193	CDD:239142	14/76 (18%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306		17/89 (19%)
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		119/389 (31%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326		7/59 (12%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310		1/2 (50%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		RecA-like_spastin	341..504	CDD:410932	70/171 (41%)
		AAA_lid_3	531..585	CDD:465537	13/53 (25%)
		Vps4_C	<578..610	CDD:462762	8/25 (32%)