DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment nub and Pou4f2

DIOPT Version :9

Sequence 1:	NP_001097153.1	Gene:	nub / 34669	FlyBaseID:	FBgn0085424	Length:	961	Species:	Drosophila melanogaster
Sequence 2:	NP_620394.2	Gene:	Pou4f2 / 18997	MGIID:	102524	Length:	411	Species:	Mus musculus

Alignment Length:	445	Identity:	138/445 - (31%)
Similarity:	194/445 - (43%)	Gaps:	103/445 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly   540 SGCGSTTPNSMAAAAAAAAAVASTM------------GSGISPLLALPGMSSPQAQLAAAGLGMN 592
            |...|.:|.|.|.||.:|::.:|:.            |.|        |.||..:...:.|.|..
Mouse    27 SALHSASPGSSAPAAPSASSPSSSSNAGGGGGGGGGGGGG--------GRSSSSSSSGSGGSGGG 83

  Fly   593 NPLLTGSLSPQDFAQFHQLLQQRQVALTQQFNSYMELLRSGSLGLAQDDPALTAQVAAAQFLMQS 657
            .    ||.:           .:|....|...|.:      |.|     |.:|.|:..|
Mouse    84 G----GSEA-----------MRRACLPTPPSNIF------GGL-----DESLLARAEA------- 115

  Fly   658 QLQALSQASQQLQALQKQQQRQVDEPLQLNHKMTQQPRSSTPHSIRSPIAIRSPASSPQQLHHHH 722
             |.|:...||..............:|....|.|...|.:|...|...||:..|..:.....||||
Mouse   116 -LAAVDIVSQSKSHHHHPPHHSPFKPDATYHTMNTIPCTSAASSSSVPISHPSALAGTHHHHHHH 179

  Fly   723 HH----PLQ---------ITPPSSAASLKLSGMLTP-----STPTSGTQMSQGTTTPQPKTVASA 769
            ||    |.|         ::|     .|.|..|..|     |||.....|:    |..|...|:.
Mouse   180 HHHHHQPHQALEGELLEHLSP-----GLALGAMAGPDGTVVSTPAHAPHMA----TMNPMHQAAL 235

  Fly   770 AAARAAGEPS-----PEETTDLEELEQFAKTFKQRRIKLGFTQGDVGLAMG--KLYG-NDFSQTT 826
            :.|.|.|.||     .:...|..:||.||:.|||||||||.||.|||.|:.  |:.| ...||:|
Mouse   236 SMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQST 300

  Fly   827 ISRFEALNLSFKNMCKLKPLLQKWLDDADRTIQATGGVFDPAALQATVSTPEII--GRRRKKRTS 889
            |.|||:|.||..||..|||:||.||::|::            :.:..::.||:.  ..:::||||
Mouse   301 ICRFESLTLSHNNMIALKPILQAWLEEAEK------------SHREKLTKPELFNGAEKKRKRTS 353

  Fly   890 IETTIRGALEKAFLANQKPTSEEITQLADRLSMEKEVVRVWFCNRRQKEKRINPS 944
            |....:.:||..|....:|:||:|..:|::|.::|.||||||||:|||:||:..|
Mouse   354 IAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYS 408

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nub	NP_001097153.1	DUF4472	315..>399	CDD:291409
		POU	791..855	CDD:197673	38/66 (58%)
		Homeobox	886..939	CDD:278475	26/52 (50%)
Pou4f2	NP_620394.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	29..95		18/88 (20%)
		Required for transcriptional activation. /evidence=ECO:0000269\|PubMed:15733064	93..239		41/173 (24%)
		POU-IV box	112..121		3/16 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	154..190		13/35 (37%)
		Nuclear speckle targeting signal. /evidence=ECO:0000250\|UniProtKB:Q12837	173..187		6/13 (46%)
		Required for DNA-binding and transcriptional repression. /evidence=ECO:0000269\|PubMed:15733064, ECO:0000269\|PubMed:7852360	240..411		77/181 (43%)
		POU	252..329	CDD:197673	41/76 (54%)
		Homeobox	350..403	CDD:278475	26/52 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3802
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810