DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nub and Pou4f2

DIOPT Version :9

Sequence 1:	NP_001097153.1	Gene:	nub / 34669	FlyBaseID:	FBgn0085424	Length:	961	Species:	Drosophila melanogaster
Sequence 2:	NP_599182.1	Gene:	Pou4f2 / 171355	RGDID:	620075	Length:	412	Species:	Rattus norvegicus

Alignment Length:	447	Identity:	138/447 - (30%)
Similarity:	194/447 - (43%)	Gaps:	106/447 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly   540 SGCGSTTPNSMAAAAAAAAAVASTM--------------GSGISPLLALPGMSSPQAQLAAAGLG 590
            |...|.:|.|.|.||.:|::.:|:.              |.|        |.||..:...:.|.|
  Rat    27 SALHSASPGSSAPAAPSASSPSSSSNAGSGGGGGGGGGGGGG--------GRSSSSSSSGSGGGG 83

  Fly   591 MNNPLLTGSLSPQDFAQFHQLLQQRQVALTQQFNSYMELLRSGSLGLAQDDPALTAQVAAAQFLM 655
            ..     ||.:           .:|....|...|.:      |.|     |.:|.|:..|
  Rat    84 GG-----GSEA-----------MRRACLPTPPSNIF------GGL-----DESLLARAEA----- 116

  Fly   656 QSQLQALSQASQQLQALQKQQQRQVDEPLQLNHKMTQQPRSSTPHSIRSPIAIRSPASSPQQLHH 720
               |.|:...||..............:|....|.|...|.:|...|...||:..|..:.....||
  Rat   117 ---LAAVDIVSQSKSHHHHPPHHSPFKPDATYHTMNTIPCTSAASSSSVPISHPSALAGTHHHHH 178

  Fly   721 HHHH----PLQ---------ITPPSSAASLKLSGMLTP-----STPTSGTQMSQGTTTPQPKTVA 767
            ||||    |.|         ::|     .|.|..|..|     |||.....|:    |..|...|
  Rat   179 HHHHHHHQPHQALEGELLEHLSP-----GLALGAMAGPDGTVVSTPAHAPHMA----TMNPMHQA 234

  Fly   768 SAAAARAAGEPS-----PEETTDLEELEQFAKTFKQRRIKLGFTQGDVGLAMG--KLYG-NDFSQ 824
            :.:.|.|.|.||     .:...|..:||.||:.|||||||||.||.|||.|:.  |:.| ...||
  Rat   235 ALSMAHAHGLPSHMGCMSDVDADPRDLEAFAERFKQRRIKLGVTQADVGSALANLKIPGVGSLSQ 299

  Fly   825 TTISRFEALNLSFKNMCKLKPLLQKWLDDADRTIQATGGVFDPAALQATVSTPEII--GRRRKKR 887
            :||.|||:|.||..||..|||:||.||::|::            :.:..::.||:.  ..:::||
  Rat   300 STICRFESLTLSHNNMIALKPILQAWLEEAEK------------SHREKLTKPELFNGAEKKRKR 352

  Fly   888 TSIETTIRGALEKAFLANQKPTSEEITQLADRLSMEKEVVRVWFCNRRQKEKRINPS 944
            |||....:.:||..|....:|:||:|..:|::|.::|.||||||||:|||:||:..|
  Rat   353 TSIAAPEKRSLEAYFAIQPRPSSEKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYS 409

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nub	NP_001097153.1	DUF4472	315..>399	CDD:291409
		POU	791..855	CDD:197673	38/66 (58%)
		Homeobox	886..939	CDD:278475	26/52 (50%)
Pou4f2	NP_599182.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	29..96		18/90 (20%)
		Required for transcriptional activation. /evidence=ECO:0000250\|UniProtKB:Q63934	94..240		41/173 (24%)
		POU-IV box	113..122		3/16 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	123..191		19/67 (28%)
		Nuclear speckle targeting signal. /evidence=ECO:0000250\|UniProtKB:Q12837	174..188		6/13 (46%)
		Required for DNA-binding and transcriptional repression. /evidence=ECO:0000250\|UniProtKB:Q63934	241..412		77/181 (43%)
		POU	253..330	CDD:197673	41/76 (54%)
		Homeobox	351..404	CDD:278475	26/52 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3802
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810