Protein Alignment eRF3 and HBS1

DIOPT Version :10

• Sequence 1: NP_477259.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Sequence 2: NP_013010.3
• Gene: HBS1 / 853959
• SGDID: S000001792
• Length: 611
• Species: Saccharomyces cerevisiae
• Alignment Length: 550
• Identity: 170/550 - (30%)
• Similarity: 277/550 - (50%)
• Gaps: 68/550 - (12%)

  Fly   117 TPSAGAAAADPTPIDGLNPSDKIAN---NETDPADSWDVDDAVITPEDEEVEDAEFTEGEATPKV 178
            ||....|||:    ...|.:.|:||   ::..|.|.  :.|.:...|.|...:.|....|.|.:.
Yeast    83 TPKKPIAAAN----GSANVTQKLANISISQQRPNDR--LPDWLDEEESEGERNGEEANDEKTVQR 141

  Fly   179 SKKKVVKVEEN---------RSKREHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREA 234
            ..|..|..:..         :|...|::.|.:|||||||||:.|:::....:|::..|.|.:||:
Yeast   142 YYKTTVPTKPKKPHDISAFVKSALPHLSFVVLGHVDAGKSTLMGRLLYDLNIVNQSQLRKLQRES 206

  Fly   235 REKSRESWYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFVPNMIGGAAQADL 299
            ....:.|:..:|.:|...|||::|.||.:..:.|.|.|.:|||:|||||:.||||.|.|.:|||:
Yeast   207 ETMGKSSFKFAWIMDQTNEERERGVTVSICTSHFSTHRANFTIVDAPGHRDFVPNAIMGISQADM 271

  Fly   300 AVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQTRYNECKDKILP 364
            |:|.:......||:|||..|||:||.:||.:.|:.:|::.:||||:  |:|.|.|:.|.|.|:||
Yeast   272 AILCVDCSTNAFESGFDLDGQTKEHMLLASSLGIHNLIIAMNKMDN--VDWSQQRFEEIKSKLLP 334

  Fly   365 YLKKLGFNPAKDLTFMPCSGLSGYGL-KDQIPETLCPWYRGPAFIPFID--------ELPSLNRK 420
            ||..:||. ..::.::|.||.||.|: |.:..:.:..||.||..:..::        |...:|:
Yeast   335 YLVDIGFF-EDNINWVPISGFSGEGVYKIEYTDEVRQWYNGPNLMSTLENAAFKISKENEGINK- 397

  Fly   421 SDGPFIMPIVD-----KYKDMGTVVMGKVESGTARKGQNLLVMPNRTQVAVDQL-------FSDD 473
             |.||:..:::     |..:...:|.||:|||:.:.|::|.:.|:.....||::       .|.:
Yeast   398 -DDPFLFSVLEIIPSKKTSNDLALVSGKLESGSIQPGESLTIYPSEQSCIVDKIQVGSQQGQSTN 461

  Fly   474 FEVTSVG-PGENVKIKLKGIEEEDVSPGFVLCDAANPIKTGKIFDAQVVILEHKSI-----ICAG 532
            .|.|.|. .|:.|.:||:....||:..|    |.|..:....|..||..:||..:.     :..|
Yeast   462 HEETDVAIKGDFVTLKLRKAYPEDIQNG----DLAASVDYSSIHSAQCFVLELTTFDMNRPLLPG 522

  Fly   533 YSAVMHIHCAAEEVTVKALICLVDKKSGDKSKTRPRFVKQDQVAIMRIECSGMICLEQFKLFP-- 595
            ...::.|....:...:|.||..:| |....||.:.|.:...|.|.:.||     .:|..:..|
Yeast   523 TPFILFIGVKEQPARIKRLISFID-KGNTASKKKIRHLGSKQRAFVEIE-----LIEVKRWIPLL 581

  Fly   596 ------QMGRFTLRDENKTIAIGKVLKVVE 619
                  ::||..||.:.:|||.||:.::.:
Yeast   582 TAHENDRLGRVVLRKDGRTIAAGKISEITQ 611

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_477259.1	PAM2	12..28	CDD:429316
		EF1_alpha	197..416	CDD:206670	90/227 (40%)
		eRF3_II	424..505	CDD:293906	25/93 (27%)
		eRF3_C_III	511..617	CDD:294003	30/118 (25%)
HBS1	NP_013010.3	HBS1_N	5..73	CDD:462642
		TEF1	166..611	CDD:444074	150/459 (33%)
		EF1_alpha	169..383	CDD:206670	89/216 (41%)