Protein Alignment eRF3 and SUP35

DIOPT Version :10

• Sequence 1: NP_477259.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Sequence 2: NP_010457.3
• Gene: SUP35 / 851752
• SGDID: S000002579
• Length: 685
• Species: Saccharomyces cerevisiae
• Alignment Length: 583
• Identity: 261/583 - (44%)
• Similarity: 370/583 - (63%)
• Gaps: 47/583 - (8%)

  Fly    75 GSTAGAAPPPPTAQGAGAAAPSAQPPTSSSNSASPAPGSP-ATTPSAGAAAADPTPIDGLNPSDK 138
            |..||..|     |..|.:....|   .....|:|.|... ....|:|...|:.|...|..|::.
Yeast   112 GYQAGFQP-----QSQGMSLNDFQ---KQQKQAAPKPKKTLKLVSSSGIKLANATKKVGTKPAES 168

  Fly   139 IANNETDPADSWD-------VDDAVITPEDEEVEDAEFTEGEA-TPKVSKKKVVKVEENRSK--- 192
            ....|...|::.:       |::.| ..|::.|:..|.||.:: .|||...|:.:...|.:.
Yeast   169 DKKEEEKSAETKEPTKEPTKVEEPV-KKEEKPVQTEEKTEEKSELPKVEDLKISESTHNTNNANV 232

  Fly   193 -------------------------REHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYER 232
                                     ::||:::|:|||||||||:||.::.|||.|||||:|||||
Yeast   233 TSADALIKEQEEEVDDEVVNDMFGGKDHVSLIFMGHVDAGKSTMGGNLLYLTGSVDKRTIEKYER 297

  Fly   233 EAREKSRESWYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFVPNMIGGAAQA 297
            ||::..|:.|||||.:|||:|||:.|||:|||:|:|||:::.:|||||||||.:|..|||||:||
Yeast   298 EAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETEKRRYTILDAPGHKMYVSEMIGGASQA 362

  Fly   298 DLAVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQTRYNECKDKI 362
            |:.||||||||||:||||:|||||||||:||||.||..:||:|||||||||||.:.||::|...:
Yeast   363 DVGVLVISARKGEYETGFERGGQTREHALLAKTQGVNKMVVVVNKMDDPTVNWSKERYDQCVSNV 427

  Fly   363 LPYLKKLGFNPAKDLTFMPCSGLSGYGLKDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIM 427
            ..:|:.:|:|...|:.|||.||.||..|||.:....||||.||..:.::|.:..::|..:.||::
Yeast   428 SNFLRAIGYNIKTDVVFMPVSGYSGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHINAPFML 492

  Fly   428 PIVDKYKDMGTVVMGKVESGTARKGQNLLVMPNRTQVAVDQLFSD-DFEVTSVGPGENVKIKLKG 491
            ||..|.||:||:|.||:|||..:|||:.|:|||:|.|.:..:::: :.||.....||.||:::||
Yeast   493 PIAAKMKDLGTIVEGKIESGHIKKGQSTLLMPNKTAVEIQNIYNETENEVDMAMCGEQVKLRIKG 557

  Fly   492 IEEEDVSPGFVLCDAANPIKTGKIFDAQVVILEHKSIICAGYSAVMHIHCAAEEVTVKALICLVD 556
            :||||:||||||....||||:...|.||:.|:|.||||.||:|.|||:|.|.|||.:..|:..::
Yeast   558 VEEEDISPGFVLTSPKNPIKSVTKFVAQIAIVELKSIIAAGFSCVMHVHTAIEEVHIVKLLHKLE 622

  Fly   557 KKSGDKSKTRPRFVKQDQVAIMRIECSGMICLEQFKLFPQMGRFTLRDENKTIAIGKVLKVVE 619
            |.:..|||..|.|.|:....|..:|....:|:|.::.:||:|||||||:..||||||::|:.|
Yeast   623 KGTNRKSKKPPAFAKKGMKVIAVLETEAPVCVETYQDYPQLGRFTLRDQGTTIAIGKIVKIAE 685

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_477259.1	PAM2	12..28	CDD:429316
		EF1_alpha	197..416	CDD:206670	133/218 (61%)
		eRF3_II	424..505	CDD:293906	42/81 (52%)
		eRF3_C_III	511..617	CDD:294003	48/105 (46%)
SUP35	NP_010457.3	PRK10263	<30..>199	CDD:236669	21/95 (22%)
		TEF1	254..685	CDD:444074	230/430 (53%)