Protein Alignment eRF3 and eIF5B

DIOPT Version :9

• Sequence 1: NP_001260415.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Sequence 2: NP_001246599.1
• Gene: eIF5B / 44261
• FlyBaseID: FBgn0026259
• Length: 1144
• Species: Drosophila melanogaster
• Alignment Length: 582
• Identity: 125/582 - (21%)
• Similarity: 206/582 - (35%)
• Gaps: 181/582 - (31%)

  Fly    87 AQGAGAAAPSAQPPTSSSNSASP--------------APGSPATTPSAGAAA------------- 124
            |||.....|:.:.||.......|              |..:.|...||.|||
  Fly   391 AQGLEIPDPNDKRPTRPGTRIRPNKKKGPKEEAAEEEAKAAEAEAQSAAAAAAAAAAEKAEEERV 455

  Fly   125 ----------ADPTPIDGLNPSDKIANNETDPADSWDVDDAVITPEDEEVEDAEFTEGEA--TPK 177
                      |:|.|.:  ..|....|.:|:.|:..:.|......:|::.:|::.::|::  :.:
  Fly   456 KESWDATDSEAEPEPEE--ETSQATNNGKTEAAEEENEDTDDDEDDDDDEDDSDDSDGDSDDSRE 518

  Fly   178 VS-----------------KKKVVKVEENRSKRE-HVNVV-FIGHVDAGKSTIGGQIMSLTGMVD 223
            ||                 .|:..:.|:.||..| ...|| .:||||.||:.|
  Fly   519 VSVLANDPESRRLRAEARILKRQAEAEKKRSTDELRAGVVCVLGHVDTGKTKI------------ 571

  Fly   224 KRTLEKYEREAREKSRESWYLSWALDTN---QEERDKGKTVEVGRAFFETDRKHFTILDAPGHKS 285
               |:|..|...:.|...........||   :..:::.|.|:.. |.||.......|:|.|||:|
  Fly   572 ---LDKLRRTHVQDSEAGGITQQIGATNVPIEAIKEQTKYVKAA-AGFEHRLPGLLIIDTPGHES 632

  Fly   286 FVPNMIGGAAQADLAVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNW 350
            |......|::..|:|:||:....| .|.      ||.|...|.|......:|.| ||: |...:|
  Fly   633 FSNLRNRGSSLCDIAILVVDIMHG-LEP------QTIESIQLLKKKKCPFIVAL-NKI-DRLYDW 688

  Fly   351 DQTRYNE-------------------CKDKILPYLKKLGFN---------PAKDLTFMPCSGLSG 387
            .|....:                   .||.||.:.:: |.|         |...::.:|.|.:||
  Fly   689 KQLARRDVRDVLKEQQSNTQLEFQQRTKDVILQFAEQ-GLNAALFYENTDPKTYISLVPTSAISG 752

  Fly   388 YGL-----------KDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIMPIVDKYKDMGTVVM 441
            .|:           ::.:.:.|.......|.:..:..||.|                   ||.:.
  Fly   753 EGMGNLLFMIADFCQNMLAKRLMYSEELQATVLEVKALPGL-------------------GTTID 798

  Fly   442 GKVESGTARKGQNLLVM----PNRTQVAVDQLFSDDFEVTSVGPGENVKIKLKGIEEEDVSPGFV 502
            ..:.:|..|:||.::|.    |..||:.         .:....|.:.:::|...:|.::|.
  Fly   799 AILINGKLREGQTMVVAGTDGPIVTQIR---------SLLMPQPMKELRVKNAYVEYKEVK---- 850

  Fly   503 LCDAANPIK-TGKIFDAQV----VILEHKS---IICAG------YSAVMHIHCAAEEVTVKA 550
               ||..:| ..|..:..:    :::.||.   .||..      .||:.||..|...|.|:|
  Fly   851 ---AAQGVKIAAKDLEKAIAGINLLIAHKPDEVEICTEEVARELKSALSHIKLAQTGVHVQA 909

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_001260415.1	PAM2	12..28	CDD:284542
		TEF1	191..619	CDD:227581	95/422 (23%)
		EF1_alpha	197..416	CDD:206670	61/261 (23%)
		eRF3_II	424..505	CDD:293906	14/84 (17%)
		eRF3_C_III	511..617	CDD:294003	14/54 (26%)
eIF5B	NP_001246599.1	PRK04004	553..1129	CDD:235195	93/418 (22%)
		IF2_eIF5B	557..769	CDD:206674	58/237 (24%)
		aeIF5B_II	779..889	CDD:293904	26/144 (18%)
		IF-2	890..993	CDD:288813	8/20 (40%)
		IF2_aeIF5B_IV	1016..1110	CDD:293911

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45464676
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	1	0.930