Protein Alignment eRF3 and sup35

DIOPT Version :10

• Sequence 1: NP_477259.1
• Gene: eRF3 / 34658
• FlyBaseID: FBgn0020443
• Length: 619
• Species: Drosophila melanogaster
• Sequence 2: NP_588225.1
• Gene: sup35 / 2539479
• PomBaseID: SPCC584.04
• Length: 662
• Species: Schizosaccharomyces pombe
• Alignment Length: 659
• Identity: 272/659 - (41%)
• Similarity: 385/659 - (58%)
• Gaps: 68/659 - (10%)

  Fly     9 ISTKFSTLNVNAVEFVPSFSYNSVVNVAEEAAAVVVAAAAAEETADPGPASGSATPA-------- 65
            :|.|..|....|..|:|||.....|.|...|.....|....:     |..|.|..|.
pombe    24 MSAKAPTFTPKAAPFIPSFQRPGFVPVNNIAGGYPYAQYTGQ-----GQNSNSPHPTKSYQQYYQ 83

  Fly    66 -TTPDSVGSGGSTA-------GAAPPPPT-------AQGAGAAAPSAQPPTSSSNSASPAPGSPA 115
             .|.::|....|..       ...||.|.       :.|...:||.:..|.|.|...:.|   |.
pombe    84 KPTGNTVDEDKSRVPDFSKKKSFVPPKPAIPKGKVLSLGGNTSAPKSTKPISISLGGTKA---PT 145

  Fly   116 TTPSAGAAAADPTPIDGLNPSDKIANNETDPADSWDVDDAVITPEDEEVE--DAEFTEGEATPKV 178
            ||..|..||...|.    .|:.|:.:..|..      :.|...|::...:  |||..:..:.|..
pombe   146 TTKPAAPAAQSKTE----TPAPKVTSESTKK------ETAAPPPQETPTKSADAELAKTPSAPAA 200

  Fly   179 SKKKVVKVEE---------------------NRSKREHVNVVFIGHVDAGKSTIGGQIMSLTGMV 222
            :.||..:..|                     :...:||||:||||||||||||:||.|:.|||||
pombe   201 ALKKAAEAAEPATVTEDATDLQNEVDQELLKDMYGKEHVNIVFIGHVDAGKSTLGGNILFLTGMV 265

  Fly   223 DKRTLEKYEREAREKSRESWYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFV 287
            ||||:||.||||:|..:||||||||||:..|||:||||||||||:|||:.:.|::|||||||.:|
pombe   266 DKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYV 330

  Fly   288 PNMIGGAAQADLAVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQ 352
            .|||.||:|||:.|||||||:||||.||:|||||||||:||:|.|:.||||::||||:|:|.|.:
pombe   331 TNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSE 395

  Fly   353 TRYNECKDKILPYLKKL-GFNPAKDLTFMPCSGLSGYGLKDQIPETLCPWYRGPAFIPFIDELPS 416
            .||.||.||:..:|::: |:|...|:.:||.|..:|..:||::..::||||:||:.:.::|.:..
pombe   396 ERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAYTGQNVKDRVDSSVCPWYQGPSLLEYLDSMTH 460

  Fly   417 LNRKSDGPFIMPIVDKYKDMGTVVMGKVESGTARKGQNLLVMPNRTQVAVDQLFSD-DFEVTSVG 480
            |.||.:.||||||..||||:||::.||:|:|:.:|..|:||||....:.|..::.: |.|::|..
pombe   461 LERKVNAPFIMPIASKYKDLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSI 525

  Fly   481 PGENVKIKLKGIEEEDVSPGFVLCDAANPIKTGKIFDAQVVILEHKSIICAGYSAVMHIHCAAEE 545
            .|:.|:::::| ::.||..|:||....||:.....|.||:.|||..||:..|||.|||||.|.||
pombe   526 CGDQVRLRVRG-DDSDVQTGYVLTSTKNPVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEE 589

  Fly   546 VTVKALICLVDKKSGDKSKTRPRFVKQDQVAIMRIECSGMICLEQFKLFPQMGRFTLRDENKTIA 610
            |:...|:..:| |:..|||..|.|..:....|..:|....:|:|:|:.:..||||||||:..|:|
pombe   590 VSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVA 653

  Fly   611 IGKVLKVVE 619
            :|||:|:::
pombe   654 VGKVVKILD 662

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_477259.1	PAM2	12..28	CDD:429316	6/15 (40%)
		EF1_alpha	197..416	CDD:206670	135/219 (62%)
		eRF3_II	424..505	CDD:293906	34/81 (42%)
		eRF3_C_III	511..617	CDD:294003	46/105 (44%)
sup35	NP_588225.1	PHA03247	72..>219	CDD:223021	33/159 (21%)
		EF1_alpha	240..462	CDD:206670	135/221 (61%)
		eRF3_II_like	468..549	CDD:293899	34/81 (42%)
		eRF3_C_III	555..660	CDD:294003	46/105 (44%)