DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eRF3 and Gspt1

DIOPT Version :9

Sequence 1:	NP_001260415.1	Gene:	eRF3 / 34658	FlyBaseID:	FBgn0020443	Length:	619	Species:	Drosophila melanogaster
Sequence 2:	NP_001003978.1	Gene:	Gspt1 / 24420	RGDID:	2751	Length:	636	Species:	Rattus norvegicus

Alignment Length:	637	Identity:	376/637 - (59%)
Similarity:	445/637 - (69%)	Gaps:	78/637 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 AAQENTEISTKFS-TLNVNAVEFVPSFSYNSVVNVAEEAAAVVVAAAAAEETADPGPASGSATPA 65
            |.:||  :|..|| .|||||..|||:      |:.||...:.:           .|||....:||
  Rat    54 AQREN--LSAAFSRQLNVNAKPFVPN------VHAAEFVPSFL-----------RGPAQPPLSPA 99

  Fly    66 TTPDSVGSGGSTAGAAPPPPTAQGAGAAAPSAQPPTSSSNSASPAPGSPATTPSAGAAAADPTPI 130
                  |:.|...||        |:.|..||  .|..||...|...||.:|   .....::|.
  Rat   100 ------GAAGGDHGA--------GSSAGGPS--EPVESSQEQSLCEGSNST---VSMELSEPV-- 143

  Fly   131 DGLNPSDKIANNETD--PADSWDVDDAVITPEDEEVEDAE----FTEGEATP------------- 176
                    :.|.||:  |.:||:        ..||:.:||    .:.|:..|
  Rat   144 --------VENGETEMSPEESWE--------HKEEISEAEPAGGGSSGDGRPPEESTQEMMEEEE 192

  Fly   177 KVSKKKVVKVEENRSKREHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSRES 241
            ::.|.|.|.......|:|||||||||||||||||||||||.|||||||||||||||||:||:||:
  Rat   193 EIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRET 257

  Fly   242 WYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFVPNMIGGAAQADLAVLVISA 306
            |||||||||||||||||||||||||:|||::||||||||||||||||||||||:|||||||||||
  Rat   258 WYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISA 322

  Fly   307 RKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQTRYNECKDKILPYLKKLGF 371
            |||||||||::|||||||||||||||||||:||:||||||||||...||.|||:|::|:|||:||
  Rat   323 RKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLIPFLKKVGF 387

  Fly   372 NPAKDLTFMPCSGLSGYGLKDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIMPIVDKYKDM 436
            ||.||:.|||||||:|..||:|  ...||||.|..|||::|.||:.||..|||..:|||||||||
  Rat   388 NPKKDIHFMPCSGLTGANLKEQ--SDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDM 450

  Fly   437 GTVVMGKVESGTARKGQNLLVMPNRTQVAVDQLFSDDFEVTSVGPGENVKIKLKGIEEEDVSPGF 501
            ||||:||:|||:..|||.|::|||:..|.|..:.|||.|..||.||||:||:|||||||::.|||
  Rat   451 GTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGF 515

  Fly   502 VLCDAANPIKTGKIFDAQVVILEHKSIICAGYSAVMHIHCAAEEVTVKALICLVDKKSGDKSKTR 566
            :|||..|...:|:.||||:||:|||||||.||:||:|||...|||.:.|||||||||||:|||||
  Rat   516 ILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTR 580

  Fly   567 PRFVKQDQVAIMRIECSGMICLEQFKLFPQMGRFTLRDENKTIAIGKVLKVV 618
            |||||||||.|.|:..:|.||||.||.||||||||||||.||||||||||:|
  Rat   581 PRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLV 632

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_001260415.1	PAM2	12..28	CDD:284542	10/16 (63%)
		TEF1	191..619	CDD:227581	323/428 (75%)
		EF1_alpha	197..416	CDD:206670	179/218 (82%)
		eRF3_II	424..505	CDD:293906	51/80 (64%)
		eRF3_C_III	511..617	CDD:294003	79/105 (75%)
Gspt1	NP_001003978.1	PAM2	62..79	CDD:284542	10/22 (45%)
		TEF1	209..633	CDD:227581	322/426 (76%)
		EF1_alpha	213..429	CDD:206670	178/217 (82%)
		eRF3_II	438..519	CDD:293906	51/80 (64%)
		eRF3_C_III	525..631	CDD:294003	79/105 (75%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	364	1.000	Domainoid score	I906
eggNOG	1	0.900	-	-		E1_COG5256
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H68226
Inparanoid	1	1.050	694	1.000	Inparanoid score	I657
OMA	1	1.010	-	-		QHG54149
OrthoDB	1	1.010	-	-		D1150082at2759
OrthoFinder	1	1.000	-	-		FOG0002347
OrthoInspector	1	1.000	-	-		otm44782
orthoMCL	1	0.900	-	-		OOG6_101550
Panther	1	1.100	-	-	LDO	PTHR23115
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X149
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	14	13.880