DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment eRF3 and GSPT2

DIOPT Version :9

Sequence 1:	NP_001260415.1	Gene:	eRF3 / 34658	FlyBaseID:	FBgn0020443	Length:	619	Species:	Drosophila melanogaster
Sequence 2:	NP_060564.2	Gene:	GSPT2 / 23708	HGNCID:	4622	Length:	628	Species:	Homo sapiens

Alignment Length:	639	Identity:	369/639 - (57%)
Similarity:	439/639 - (68%)	Gaps:	71/639 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MAAQENTEISTKFS-TLNVNAVEFVPSFSYNSVVNVAEEAAAVVVAAAAAEETADPGPASGSATP 64
            :|..:...:|:.|| .|||||..|||:      |:.||..               |....|...|
Human    36 VAEAQREPLSSAFSRKLNVNAKPFVPN------VHAAEFV---------------PSFLRGPTQP 79

  Fly    65 ATTPDSVGSGGST-AGAAPPPPTAQGAGA-AAPSAQPPTSSSNSASPAPGSPATTPSAGAAAADP 127
            .|.|...||...| .||..|.....|.|| ..||.:.|..|...::.|.....:.|
Human    80 PTLPAGSGSNDETCTGAGYPQGKRMGRGAPVEPSREEPLVSLEGSNSAVTMELSEP--------- 135

  Fly   128 TPIDGLNPSDKIANNETDPA--DSWDVDDAVITPEDEEVEDAE---FTEGEATP----------- 176
                      .:.|.|.:.|  :||:        ..:||.:||   .:.|::.|
Human   136 ----------VVENGEVEMALEESWE--------HSKEVSEAEPGGGSSGDSGPPEESGQEMMEE 182

  Fly   177 --KVSKKKVVKVEENRSKREHVNVVFIGHVDAGKSTIGGQIMSLTGMVDKRTLEKYEREAREKSR 239
              ::.|.|.|.|.....|:|||||||||||||||||||||||.|||||||||||||||||:||:|
Human   183 KEEIRKSKSVIVPSGAPKKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNR 247

  Fly   240 ESWYLSWALDTNQEERDKGKTVEVGRAFFETDRKHFTILDAPGHKSFVPNMIGGAAQADLAVLVI 304
            |:|||||||||||||||||||||||||:|||:|||||||||||||||||||||||:|||||||||
Human   248 ETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVI 312

  Fly   305 SARKGEFETGFDRGGQTREHAMLAKTAGVKHLVVLVNKMDDPTVNWDQTRYNECKDKILPYLKKL 369
            |||||||||||::|||||||||||||||||||:||:||||||||||...||.|||:|::|:|||:
Human   313 SARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKV 377

  Fly   370 GFNPAKDLTFMPCSGLSGYGLKDQIPETLCPWYRGPAFIPFIDELPSLNRKSDGPFIMPIVDKYK 434
            ||:|.||:.|||||||:|..:|:|  ...||||.|..|||::|.||:.||..|||..:|||||||
Human   378 GFSPKKDIHFMPCSGLTGANIKEQ--SDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVDKYK 440

  Fly   435 DMGTVVMGKVESGTARKGQNLLVMPNRTQVAVDQLFSDDFEVTSVGPGENVKIKLKGIEEEDVSP 499
            ||||||:||:|||:..|||.|::|||:..|.|..:.|||.|...|.||||:||:|||||||::.|
Human   441 DMGTVVLGKLESGSIFKGQQLVMMPNKHNVEVLGILSDDTETDFVAPGENLKIRLKGIEEEEILP 505

  Fly   500 GFVLCDAANPIKTGKIFDAQVVILEHKSIICAGYSAVMHIHCAAEEVTVKALICLVDKKSGDKSK 564
            ||:|||.:|...:|:.||.|:||:|||||||.||:||:|||...|||.:.|||.|||||||:|||
Human   506 GFILCDPSNLCHSGRTFDVQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALISLVDKKSGEKSK 570

  Fly   565 TRPRFVKQDQVAIMRIECSGMICLEQFKLFPQMGRFTLRDENKTIAIGKVLKVV 618
            |||||||||||.|.|:..:|.||||.||.||||||||||||.||||||||||:|
Human   571 TRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLV 624

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_001260415.1	PAM2	12..28	CDD:284542	10/16 (63%)
		TEF1	191..619	CDD:227581	319/428 (75%)
		EF1_alpha	197..416	CDD:206670	178/218 (82%)
		eRF3_II	424..505	CDD:293906	50/80 (63%)
		eRF3_C_III	511..617	CDD:294003	77/105 (73%)
GSPT2	NP_060564.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..49		2/12 (17%)
		PAM2	47..64	CDD:284542	10/22 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	72..124		17/51 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	146..195		11/56 (20%)
		TEF1	201..625	CDD:227581	318/426 (75%)
		EF1_alpha	205..421	CDD:206670	177/217 (82%)
		G1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	210..217		6/6 (100%)
		G2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	266..270		3/3 (100%)
		G3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	287..290		2/2 (100%)
		G4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	349..352		2/2 (100%)
		G5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01059	391..393		1/1 (100%)
		eRF3_II	430..511	CDD:293906	50/80 (63%)
		eRF3_C_III	517..623	CDD:294003	77/105 (73%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	365	1.000	Domainoid score	I935
eggNOG	1	0.900	-	-		E1_COG5256
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	688	1.000	Inparanoid score	I702
Isobase	1	0.950	-	0	Normalized mean entropy	S365
OMA	1	1.010	-	-		QHG54149
OrthoDB	1	1.010	-	-		D1150082at2759
OrthoFinder	1	1.000	-	-		FOG0002347
OrthoInspector	1	1.000	-	-		otm40644
orthoMCL	1	0.900	-	-		OOG6_101550
Panther	1	1.100	-	-	O	PTHR23115
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X149
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.790