DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eRF3 and HBS1

DIOPT Version :9

Sequence 1:	NP_001260415.1	Gene:	eRF3 / 34658	FlyBaseID:	FBgn0020443	Length:	619	Species:	Drosophila melanogaster
Sequence 2:	NP_001286906.1	Gene:	HBS1 / 117365	FlyBaseID:	FBgn0042712	Length:	670	Species:	Drosophila melanogaster

Alignment Length:	543	Identity:	188/543 - (34%)
Similarity:	284/543 - (52%)	Gaps:	32/543 - (5%)

- Green bases have known domain annotations that are detailed below.

  Fly    85 PTAQGAGAAAPSAQPPTSSSNSASPAPGSPATTPSAGAAAADPTPIDGLNPSDKIANNETDPADS 149
            |......|.|....|.|.|....:|.|......|..|.....|..     ||..:.:....|.|.
  Fly   149 PAVNKMKAPAAPVLPKTVSKTVPTPPPKISLKEPRRGFEIPSPKV-----PSSPVVSGRNTPVDI 208

  Fly   150 WDVDDAVITPEDEEVEDAEFTEGEATP-KVSKKKVVK------VEENRSKREHVNVVFIGHVDAG 207
            ...||              .:...||. ||||::.|:      .:|...::.|::::.|||||||
  Fly   209 SAGDD--------------ISRSSATVFKVSKEQAVRNARQLYEKERADQKSHIHMIVIGHVDAG 259

  Fly   208 KSTIGGQIMSLTGMVDKRTLEKYEREAREKSRESWYLSWALDTNQEERDKGKTVEVGRAFFETDR 272
            |||:.|.::..||.|.:|.:.|:|:|:::..::|:..:|.||...|||.:|.|::||::..||..
  Fly   260 KSTLMGHLLYDTGNVSQRVMHKHEQESKKLGKQSFMYAWVLDETGEERARGITMDVGQSRIETKT 324

  Fly   273 KHFTILDAPGHKSFVPNMIGGAAQADLAVLVISARKGEFETGFDRGGQTREHAMLAKTAGVKHLV 337
            |..|:|||||||.|:||||.||.|||:|:||:.|.:||||:||:.||||||||:|.::.||..|.
  Fly   325 KIVTLLDAPGHKDFIPNMISGATQADVALLVVDATRGEFESGFELGGQTREHAILVRSLGVNQLG 389

  Fly   338 VLVNKMDDPTVNWDQTRYNECKDKILPYLKKLGFNPAKDLTFMPCSGLSGYGLKDQIPE-TLCPW 401
            |::||:|  ||.|.|.|:.|...|:..:||..||..: |::|.|||||:|..|..:..| .|..|
  Fly   390 VVINKLD--TVGWSQDRFTEIVTKLKSFLKLAGFKDS-DVSFTPCSGLTGENLTKKAQEPALTNW 451

  Fly   402 YRGPAFIPFIDELPSLNRKSDGPFIMPIVDKYKDMGT--VVMGKVESGTARKGQNLLVMPNRTQV 464
            |.|...:..|:......|..|.|..|.:.|.||..|:  .:.|:||:|.......:||..:|.|.
  Fly   452 YSGRHLLDVIENFKIPERAIDRPLRMSVSDIYKGTGSGFCISGRVETGVLCLNDKVLVGASREQA 516

  Fly   465 AVDQLFSDDFEVTSVGPGENVKIKLKGIEEEDVSPGFVLCDAANPIKTGKIFDAQVVILEHKSII 529
            .|..|..::|..|.|..|:.|.:.|..::..:|:.|.::.|...||.....|.|::::...|..|
  Fly   517 QVKSLTMNEFPQTCVFAGDQVSVTLPALDINNVTVGCIISDPQTPIPVTTRFQARIIVFNVKVPI 581

  Fly   530 CAGYSAVMHIHCAAEEVTVKALICLVDKKSGDKSKTRPRFVKQDQVAIMRIECSGMICLEQFKLF 594
            ..|:..::|.....|...|..|...:.|.:|:..|.:||.:..:..|::.:|.|..||:|::..|
  Fly   582 TMGFPVLLHHQSLIEPAVVCKLTASIHKSTGEVVKKKPRCLGNNSCALVELETSRPICIERYADF 646

  Fly   595 PQMGRFTLRDENKTIAIGKVLKV 617
            .::||..||....|||.|.|.|:
  Fly   647 KELGRVMLRVAGVTIAAGMVTKI 669

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eRF3	NP_001260415.1	PAM2	12..28	CDD:284542
		TEF1	191..619	CDD:227581	163/430 (38%)
		EF1_alpha	197..416	CDD:206670	102/219 (47%)
		eRF3_II	424..505	CDD:293906	24/82 (29%)
		eRF3_C_III	511..617	CDD:294003	30/105 (29%)
HBS1	NP_001286906.1	HBS1_N	83..145	CDD:286079
		TEF1	241..669	CDD:227581	162/430 (38%)
		EF1_alpha	249..468	CDD:206670	102/221 (46%)
		HBS1-like_II	474..557	CDD:293912	24/82 (29%)
		HBS1_C_III	561..669	CDD:294008	32/107 (30%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45464670
Domainoid	1	1.000	131	1.000	Domainoid score	I241
eggNOG	1	0.900	-	-		E1_COG5256
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	153	1.000	Inparanoid score	I210
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1150082at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm51361
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	P	PTHR23115
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	8	7.900