Protein Alignment EYS and Agrn

DIOPT Version :10

• Sequence 1: NP_001278938.1
• Gene: EYS / 346007
• HGNCID: 21555
• Length: 3165
• Species: Homo sapiens
• Sequence 2: XP_006538554.1
• Gene: Agrn / 11603
• MGIID: 87961
• Length: 2057
• Species: Mus musculus
• Alignment Length: 3105
• Identity: 582/3105 - (18%)
• Similarity: 876/3105 - (28%)
• Gaps: 1337/3105 - (43%)

Human    23 TCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNT-------------KIDTSGNQAV 74
            ||..:.:|. ..:.::.|:..|:.|.:.:|..:..:...|..             .:...||:.|
Mouse    32 TCPERALER-REEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDVVAQESLLDGGNKVV 95

Human    75 ------PQICPLQIQLGD--ILVISSEPSLQFP-------------EINLMNVSETSFVGCVQNT 118
                  |.||..|:..||  |..::..|...:|             .|.|.|:.|..|  ||::
Mouse    96 IGGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEF--CVED- 157

Human   119 TTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFC 183
                                   ..|.|:     .:.||
Mouse   158 -----------------------KPGIHF-----TAAPS-------------------------- 168

Human   184 SGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPF 248
                               .||         |.|....|                .:..|
Mouse   169 -------------------MPP---------DVCRGMLC----------------GFGAV----- 184

Human   249 TGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSS 313
                       |:|.|...|..|        |.|.:.    .|.....|          :|.:.:
Mouse   185 -----------CEPSVEDPGRAS--------CVCKKN----VCPAMVAP----------VCGSDA 216

Human   314 SAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPI----FTDLLCKSIQTSC 374
            |.|:.||....:..|.:..:......||.:...|..::......|.|.    ....||   .|:|
Mouse   217 STYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLC---PTTC 278

Human   375 ESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPG 439
            ...|  :...|.....|||                ..|:||...|.|:|   :|..:|...|.|
Mouse   279 FGAP--DGTVCGSDGVDYP----------------SECQLLRHACANQE---HIFKKFDGPCDP- 321

Human   440 C----------------TKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGS 488
            |                |::|...|:......||...||        |.|
Mouse   322 CQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICG--------DDG--------------- 363

Human   489 EGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYL 553
                                  .||.||       |.....|    .|.|.........|
Mouse   364 ----------------------VTYEND-------CVMSRIG----AARGLLLQKVRSGQ----- 390

Human   554 CFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRCSCSLSYIGRLCVVNVDYCLGNHSISVHG 618
            |           .|.|.....||..:||.....||.|||.
Mouse   391 C-----------QTRDQCPETCQFNSVCLSRRGRPHCSCD------------------------- 419

Human   619 LCLALSHNCNCSGLQRYERNICEID----TEDC--KSASCKNGTTSTHLRGYFFRKCVPGFKGTQ 677
                   ...|.|..   |.:|..|    ..||  :.|.|:...|            :|......
Mouse   420 -------RVTCDGAY---RPVCAQDGHTYDNDCWRQQAECRQQQT------------IPPKHQGP 462

Human   678 CEIDIDECASHPCKNGATCIDQPGNYFCQCVPPFKVVDGFSCLCNPGYVGIRCEQDIDDCILNAC 742
            |:.....|....|..||||..:.|...|:|          ..:|:.|
Mouse   463 CDQTPSPCRGAQCAFGATCTVKNGKAVCEC----------QRVCSGG------------------ 499

Human   743 EHNSTCKDLHLSYQCVCLSD--WEGNFCEQESNEC---------KMNPCKNNSTCTDLYKSYRCE 796
                        |..||.||  ..|:.||.||..|         :..||.            ||
Mouse   500 ------------YDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCD------------RC- 539

Human   797 CTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCR 861
                  ||             |..|.||...|  |:  |:||
Mouse   540 ------GQ-------------CRFGSLCEVET--GR--CVCP----------------------- 558

Human   862 NNSTCLALVDANQHCICREEFEGKNCEIDVKDC-----LFLS----CQDYGDCEDMVNNFRCICR 917
              |.|:.  .|...|..........||:.|..|     |:::    ||   .|.:.|..|..:|
Mouse   559 --SECVE--SAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQ---TCGETVCTFGAVC- 615

Human   918 PGFSGSLCEIEINECSSEPCKNNGTCVDLTNRFFC-NCEPEYHGPFCELDVNKCKISPC-LDEEN 980
                                 :.|.||       | .||....||.|..| ....:|.| |.|..
Mouse   616 ---------------------SAGQCV-------CPRCEHPPPGPVCGSD-GVTYLSACELREAA 651

Human   981 CVYRTDGYNCLCAPGYTGINCEINLDECLSEPCLHDGVCIDGINHYTCDCKSGFFGTHCETNAND 1045
            |                  ..::.::|..:.||            ...:|.||..|:. |.||
Mouse   652 C------------------QQQVQIEEARAGPC------------EPAECGSGGSGSG-EDNA-- 683

Human  1046 CLSNPC-LHGRC-TELINEYPCSCD-------------ADGT--STQCKIKINDCTS-------- 1085
            |....| .||.. .|...:.||.||             :||.  ||:|.:|...|.:
Mouse   684 CEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAA 748

Human  1086 ------------IPCMNE-------GFCQ---KSAHGF-------TCIC-PRGYTGAYCEKSIDN 1120
                        :|..:.       |.||   .:|.|.       ||.| |.|.....|:.....
Mouse   749 QGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQ 813

Human  1121 CAEPELNSVICLNGGICVDGPGHTFDCRCLPGF-----------SGQFCEININECSSSPCLHGA 1174
            |:              |..|.|.....||.|||           ||  |    ..||..|  .||
Mouse   814 CS--------------CRPGVGGLRCDRCEPGFWNFRGIVTDGHSG--C----TPCSCDP--RGA 856

Human  1175 ---DCEDHINGYVCKCQPGWSGHHCENELECIPNSCVHELCMENEPGSTCLCTPGFMTCSIGLLC 1236
               ||| .:.| :|.|:||.:|..|.   :|.....:..|..|.:|.:...|..  |.|..|..|
Mouse   857 VRDDCE-QMTG-LCSCRPGVAGPKCG---QCPDGQALGHLGCEADPTTPVTCVE--MHCEFGASC 914

Human  1237 GDEIRRITCLTPIFQRTDPISTQTYTIP-PSETLVSSFPSI---KATRIPAIMDTYPVDQGPKQT 1297
            .:|.....|:.|           |.|.| .:.|.|.....:   ...::..|.....:|...:..
Mouse   915 VEEAGFAQCVCP-----------TLTCPEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSL 968

Human  1298 GIVKHDILPTTGLATLRISTPLESYLLQELIVTRELSAKHSLLSSADVSSSRFLNFGIRDPAQIV 1362
            |..:..:.|.....:..::||..       |::|.|::.||.|..:              |:...
Mouse   969 GPCRESVAPGVSPTSASMTTPRH-------ILSRTLASPHSSLPLS--------------PSTTA 1012

Human  1363 QDKTSVSHMPIRTSAATLGFFFPDRRARTPFIMSSLMSDFIFPTQSLLFENCQTVALSATPTTSV 1427
            .|..:    |:.||..|: ...|...|.||..::||                         .|::
Mouse  1013 HDWPT----PLPTSPQTV-VGTPRSTAATPSDVASL-------------------------ATAI 1047

Human  1428 IRSIPGADIELNRQSLLSRGFLLIAASISATPVVSRGAQEDIEEYSADSLISRREHWRLLSPSMS 1492
            .|. .|:                           :.|:.:  ||.|.|...|......|..|..|
Mouse  1048 FRE-SGS---------------------------TNGSGD--EELSGDEEASGGGSGGLEPPVGS 1082

Human  1493 PIFPAKVIISKQVTILNSSALHRFSTKAFNPSEYQAITEASSNQRLTNIKSQAADSLRELSQTCA 1557
                        |.:.:...:.|.|.       |.:.....|:.:..::.|:.::
Mouse  1083 ------------VVVTHGPPIERASC-------YNSPLGCCSDGKTPSLDSEGSN---------- 1118

Human  1558 TCSMTEIKSSREFSDQVLHSKQSHFYETFWMNSAILASWYALMGAQTITSGHSFSSATEITPSVA 1622
             |..|:                                              :|....|:..
Mouse  1119 -CPATK----------------------------------------------AFQGVLELEG--- 1133

Human  1623 FTEVPSLF--PSKKSAKRTILSSSLEESITLSSNLDVNLCLDKTCLSIVPSQTISSDLM-NSDLT 1684
             .|...||  |.....|..:..   |.:.::.|.||                    ||. |||:.
Mouse  1134 -VEGQELFYTPEMADPKSELFG---ETARSIESTLD--------------------DLFRNSDVK 1174

Human  1685 SKMTTDELSVSENILKLLKIRQYGITMGPTEVLNQESLLDMEKSKGSHTLFKLHPSDSSLDFELN 1749
            ....:            :::|:    :||.:::  .:::|:.             .|.:..|:.
Mouse  1175 KDFWS------------IRLRE----LGPGKLV--RAIVDVH-------------FDPTTAFQA- 1207

Human  1750 LQIYPDVTLKTYSEITHANDFKNNLPPLTGSVPDFSEVTTNVAFYTVSATPALSIQTSSSMSVIR 1814
                |||......:|                              .||...||:::        |
Mouse  1208 ----PDVGQALLQQI------------------------------QVSRPWALAVR--------R 1230

Human  1815 P----------DW--PYFTDYMTSLKKEVKTSSEWSKWELQPSVQYQEFPTASRHLPFTRSLTLS 1867
            |          ||  .:||...|.....|.|:                           |:.|:|
Mouse  1231 PLREHVRFLDFDWFPTFFTGAATGTTAAVATA---------------------------RATTVS 1268

Human  1868 SLESILAPQRLMISDFSCVRYYGDSYLEFQNVALNPQNNISLEFQTFSSYGLLLYVKQDSNLVDG 1932
            .|.:.....|:                                   :.||
Mouse  1269 RLSASSVTPRV-----------------------------------YPSY--------------- 1283

Human  1933 FFIQLFIENGTLKYHFYCPGEAKFKSINTTVRVDNGQKYTLLIRQELDPCNAELTILGRNTQICE 1997
                                                                             
Mouse  1284 ----------------------------------------------------------------- 1283

Human  1998 SINHVLGKPLPKSGSVFIGGFPDLHGKIQMPVPVKNFTGCIEVIEINNWRSFIPSKAVKNYHINN 2062
                 ..:|:               |:...|:..:.
Mouse  1284 -----TSRPV---------------GRTTAPLTTRR----------------------------- 1299

Human  2063 CRSQGFMLSPTASFVDASDVTQGVDTMWTSVSPSVAAPSVCQQDVCHNGGTCHAIFLSSGIVSFQ 2127
                    .||        .|..:|...|. .|. ..|..|....|.:||||..:....|   |.
Mouse  1300 --------PPT--------TTASIDRPRTP-GPQ-RPPKSCDSQPCLHGGTCQDLDSGKG---FS 1343

Human  2128 CDCPLHFTGRFCEKDAGLFFPSFNGNSYLELPFLKFVLEKEHNRTVTIYLTIKTNSLNGTILYSN 2192
            |.|.....|..|||......|:|.|:|:|..|.|:..      .|:.:.|..:.....|.:|| |
Mouse  1344 CSCTAGRAGTVCEKVQLPSVPAFKGHSFLAFPTLRAY------HTLRLALEFRALETEGLLLY-N 1401

Human  2193 GNNCGKQFLHLFLVEGRPSVKYGCGNSQNILTVSANYSINTNAFTPITIRYTTPVGSPGVVCMIE 2257
            ||..||.||.|.|::|....::..|:...:||          :..|:         .||....:|
Mouse  1402 GNARGKDFLALALLDGHVQFRFDTGSGPAVLT----------SLVPV---------EPGRWHRLE 1447

Human  2258 ---------MTADGKPPVQKK----------DTEISHASQAYFESMFLGHIP----ANVQIHKKA 2299
                     ::.||:.||..:          ||:           :::|.:|    |.|......
Mouse  1448 LSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTK-----------LYVGGLPEEQVATVLDRTSV 1501

Human  2300 GPVYGFRGCILDLQVNNKEFFIIDEAR---HGKNIENCHVPWCAHHLCRNNGTCISDNENLF-CE 2360
            |  .|.:|||..|.:||::..:.|..|   ....:..|....|:.:.|.....|.:....:| |:
Mouse  1502 G--IGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQ 1564

Human  2361 CPRLYSGKLC--QFASCENNPCGNGATCVPKSGTDIVCLCPYGRSGPLCTDAINI--TQPRFSGT 2421
            ||....|..|  :...|:.|||...|.|...|.....|.||.||||..|...:..  ::|..:..
Mouse  1565 CPPGRFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADF 1629

Human  2422 DAFGYTSFLAYSRIS-DISFHYEFHLKFQLANNHSALQNNLIFFTGQKGHGLNGDDFLAVGLLNG 2485
            :.|.|.......... |:.......:.| ||...|    .|:.:.|||..|..  ||:::.|.|.
Mouse  1630 NGFSYLELKGLHTFERDLGEKMALEMVF-LARGPS----GLLLYNGQKTDGKG--DFVSLALHNR 1687

Human  2486 SVVYSYNLGSGIASIRS-EPLNLSLGVHTVHLGKFFQEGWLKVDD------HKNKSIIAPGRLVG 2543
            .:.:.|:||.|.|.||| ||:.|...|. |.|.:..::|.|:|.|      ...||...|..:
Mouse  1688 HLEFRYDLGKGAAIIRSKEPIALGTWVR-VFLERNGRKGALQVGDGPRVLGESPKSRKVPHTM-- 1749

Human  2544 LNVFSQFYVGGYSEYTPDLLPNGADFKNGFQGCIFTLQVRTEKDGH--------FRGLGNPEGHP 2600
            ||:....||||..:::.  |..||...:||.|.|..:.:|    ||        .|.:   :..|
Mouse  1750 LNLKEPLYVGGAPDFSK--LARGAAVASGFDGAIQLVSLR----GHQLLTQEHVLRAV---DVAP 1805

Human  2601 NAGRSVGQCHASPCSLMKCGNGGTCIESGTSVYCNCTTGWKGSFCTETVSTCDPEHDPPHHCSRG 2665
            .||....|...:||.     |||:||                                    .|.
Mouse  1806 FAGHPCTQAVDNPCL-----NGGSCI------------------------------------PRE 1829

Human  2666 ATCISLPHGYTCFCPLGTTGIYCEQALI--------------------LIVILEKPK----PAER 2706
            ||       |.|.||.|.:|::||:.::                    |..:.|...    ||..
Mouse  1830 AT-------YECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPE 1887

Human  2707 KVKKEALSISDPSFRSNELSWMSFASFHVRKKTHIQLQFQPLAADGILFYAAQHLKAQSGDFLCI 2771
            .:...|| .|:.:.:||                |.:|..:..|..|::.:..:  ..:..|::.:
Mouse  1888 TLDSRAL-FSEKALQSN----------------HFELSLRTEATQGLVLWIGK--VGERADYMAL 1933

Human  2772 SLVNSSVQLRYNLGDRTIILETLQKVTINGSTWHIIKAGRVGAEGYLDL-DGINVTEKASTKMSS 2835
            ::|:..:||.|:||.:.::|.:..||  |.:.|..::|.|...||.|.: :...||..:....:.
Mouse  1934 AIVDGHLQLSYDLGSQPVVLRSTVKV--NTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQ 1996

Human  2836 LDTNTDFYIGGVSSLNL--VNPMAIENEPVGFQGCIRQVIINNQELQLTE 2883
            |||:...::||:..|.:  ..|.|.   ..||.||:|.|::.:::|.|.|
Mouse  1997 LDTDGALWLGGLQKLPVGQALPKAY---GTGFVGCLRDVVVGHRQLHLLE 2043

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EYS	NP_001278938.1	EGF_CA	215..253	CDD:238011	4/37 (11%)
		EGF_CA	681..731	CDD:238011	11/49 (22%)
		EGF_CA	733..769	CDD:214542	6/37 (16%)
		EGF_CA	773..806	CDD:238011	7/41 (17%)
		EGF_CA	810..846	CDD:238011	9/35 (26%)
		EGF_CA	890..926	CDD:238011	9/44 (20%)
		EGF_CA	929..964	CDD:238011	8/35 (23%)
		EGF_CA	966..1002	CDD:238011	6/36 (17%)
		EGF_CA	1004..1040	CDD:238011	7/35 (20%)
		EGF_CA	1080..1115	CDD:238011	12/72 (17%)
		EGF_CA	1118..1159	CDD:238011	11/51 (22%)
		EGF_CA	1161..1197	CDD:238011	14/38 (37%)
		LamG	1886..2044	CDD:238058	5/157 (3%)
		LamG	2149..2315	CDD:238058	44/188 (23%)
		LamG	2459..2582	CDD:214598	43/129 (33%)
		EGF	2614..2644	CDD:394967	6/29 (21%)
		EGF_CA	2657..2689	CDD:238011	9/31 (29%)
		LamG	2740..2877	CDD:214598	37/139 (27%)
		LamG	2977..3138	CDD:238058
Agrn	XP_006538554.1	NtA	33..148	CDD:460825	21/115 (18%)
		KAZAL	198..244	CDD:197624	9/59 (15%)
		KAZAL	274..319	CDD:197624	16/68 (24%)
		Kazal_1	351..391	CDD:395004	15/100 (15%)
		KAZAL_FS	423..463	CDD:238052	11/54 (20%)
		KAZAL	491..536	CDD:197624	14/84 (17%)
		KAZAL	556..601	CDD:197624	11/71 (15%)
		KAZAL	621..666	CDD:197624	14/70 (20%)
		KAZAL	706..752	CDD:197624	9/45 (20%)
		Laminin_EGF	795..837	CDD:395007	14/55 (25%)
		Laminin_EGF	849..>885	CDD:395007	16/42 (38%)
		KAZAL	924..971	CDD:197624	9/57 (16%)
		SEA	1121..1245	CDD:214554	35/270 (13%)
		EGF	1322..1352	CDD:394967	10/32 (31%)
		Laminin_G_1	1389..1520	CDD:395008	38/163 (23%)
		Laminin_G_1	1657..1792	CDD:395008	50/150 (33%)
		EGF_CA	1814..1846	CDD:238011	16/79 (20%)
		Laminin_G_1	1910..2040	CDD:395008	35/136 (26%)