Protein Alignment Mal-B2 and IMA4

DIOPT Version :10

• Sequence 1: NP_723693.3
• Gene: Mal-B2 / 34598
• FlyBaseID: FBgn0032382
• Length: 583
• Species: Drosophila melanogaster
• Sequence 2: NP_012314.1
• Gene: IMA4 / 853235
• SGDID: S000003757
• Length: 589
• Species: Saccharomyces cerevisiae
• Alignment Length: 614
• Identity: 186/614 - (30%)
• Similarity: 291/614 - (47%)
• Gaps: 110/614 - (17%)

  Fly    34 WWPHTVFYQIYPRSFKDSNGDGIGDLKGITSKLRYLADTGITATWLSPIFQSPMIDFGYDISDYK 98
            ||.....|||||.||||||.||.||:|||.|||.|:.:.|..|.|:||.:.||..|.||||::|:
Yeast    14 WWKEATIYQIYPASFKDSNNDGWGDMKGIASKLEYIKELGTDAIWISPFYDSPQDDMGYDIANYE 78

  Fly    99 AIQPEYGTMQDFEELIDTAFELGIKVVLDFVPNHSSDQHEWFKKS-AAREPGYEDFYVW-----H 157
            .:.|.|||.:|...||:...:||:|.:.|.|.||.|.:|||||:| :::.....|::.|     :
Yeast    79 KVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGY 143

  Fly   158 DGIVQENGTRVPPNNWPSVFYGSAWEWHEGREQYYLHQFTKEQPDLNYRNPKVVQAM-DDVLLFW 221
            |.    .|..:|||||.|.|.||||.:.|..:::||..|...|||||:.|....:|: :..:.:|
Yeast   144 DA----EGKPIPPNNWRSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYW 204

  Fly   222 LNKGVAGFRIDAVNHLFEDESLKDEPLSGKTTD-SLSYDYTK-----HIYSRDLPE-VLEMIHHW 279
            |:.||.|||||..:...:...|.|.|:..:.:. .||..:|.     |.:.:::.: :...:...
Yeast   205 LDHGVDGFRIDVGSLYSKVAGLPDAPVIDENSKWQLSDPFTMNGPRIHEFHQEMNKFIRNRVKDG 269

  Fly   280 RQLLDDFSAKHPERPTRIMMTEAYA-GLTQLADYYEDSNGVRGSHLPFNFHFITDVKGDSDARDY 343
            |:::.....:|....|:.:.|.|.. .|::|                |||.. |||......|..
Yeast   270 REIMTVGEMRHATDETKRLYTSASRHELSEL----------------FNFSH-TDVGTSPKFRQN 317

  Fly   344 V--YNVEKWLIYMPRGHAANWVMG----------NHDNPRVASRFGP-------ASVDAMNMLLL 389
            :  |.::.|.:.:  .....:|.|          |||.||..:|||.       .|...:::||:
Yeast   318 LIPYELKDWKVAL--AELFRYVNGTDCWSTIYLENHDQPRSITRFGDDSPKNRVISGKLLSVLLV 380

  Fly   390 TLPGVAVTYNGEELGMVDYRELSWEETVDPPARNVGEKLYQE-----------------VSRDPV 437
            :|.|....|.|:|||.::::....|:..|...||..:.:.:|                 :|||..
Yeast   381 SLSGTLYVYQGQELGEINFKNWPIEKYEDVEVRNNYDAIKEEHGENSKEMKRFLEAIALISRDHA 445

  Fly   438 RTPFQWN-NETNAGFS-TAAKTWLPVHPNYLE-LNLEAQKVANRSHYQVYKDLLELRKS-AIMRV 498
            |||.||: .|.||||| ..||.|..::.::.| :|.|.:.....|....:|:.|..||: ..:.|
Yeast   446 RTPMQWSREEPNAGFSGPNAKPWFYLNESFREGINAEDESKDPNSVLNFWKEALRFRKAHKDITV 510

  Fly   499 GRFNIEPL---TRWVFAFKRSYPNFESIITVINVSDKEQLVDLS----------EFLSQPKKLVV 550
            ..::.|.:   .:.:|:|.:.|.| :::...:|.|...  :|.:          ||.:.|:
Yeast   511 YGYDFEFIDLDNKKLFSFTKKYDN-KTLFAALNFSSDS--IDFTIPNNSSSFKLEFGNYPR---- 568

  Fly   551 EVSGVDSKYQPGQSLAASALLLAAREGLV 579
              |.||          ||:..|...||.:
Yeast   569 --SEVD----------ASSRTLKPWEGRI 585

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mal-B2	NP_723693.3	AmyAc_maltase	33..502	CDD:200467	167/522 (32%)
IMA4	NP_012314.1	AmyAc_SI_OligoGlu_DGase	16..503	CDD:200472	162/509 (32%)
		Malt_amylase_C	514..585	CDD:435493	19/89 (21%)