DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Mal-B2 and IMA5

DIOPT Version :9

Sequence 1:	NP_001188791.2	Gene:	Mal-B2 / 34598	FlyBaseID:	FBgn0032382	Length:	583	Species:	Drosophila melanogaster
Sequence 2:	NP_012319.1	Gene:	IMA5 / 853214	SGDID:	S000003752	Length:	581	Species:	Saccharomyces cerevisiae

Alignment Length:	577	Identity:	185/577 - (32%)
Similarity:	280/577 - (48%)	Gaps:	112/577 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    34 WWPHTVFYQIYPRSFKDSNGDGIGDLKGITSKLRYLADTGITATWLSPIFQSPMIDFGYDISDYK 98
            ||.....|||||.||||||.||.|||.||||||.|:.:.|:.|.|:.|.:.||..|.||||::|:
Yeast     9 WWKEATVYQIYPASFKDSNNDGWGDLAGITSKLDYVKELGVDAIWVCPFYDSPQEDMGYDIANYE 73

  Fly    99 AIQPEYGTMQDFEELIDTAFELGIKVVLDFVPNHSSDQHEWFKKS-AAREPGYEDFYVW-----H 157
            .:.|.|||.:|..::|:.|.:.||||::|.|.||.|::|||||:| :::.....|::.|     :
Yeast    74 KVWPRYGTNEDCFQMIEEAHKRGIKVIVDLVINHCSEEHEWFKESRSSKANPKRDWFFWRPPKGY 138

  Fly   158 DGIVQENGTRVPPNNWPSVFYGSAWEWHEGREQYYLHQFTKEQPDLNYRNPKVVQAM-DDVLLFW 221
            |    |.|..:|||||.|.|.||||.:.|...:::||.|...|||.|:.|.:..:|: |..:.:|
Yeast   139 D----EKGNPIPPNNWRSFFGGSAWRYDEKTGEFFLHVFALGQPDFNWENEECRKAIYDSSVGYW 199

  Fly   222 LNKGVAGFRIDAVNHLFEDESLKDEPLSGKTTDSLSYDYTK-----------HIYSRDLPE-VLE 274
            |...|.|||||..:...:.|.|.|.|::..|.     .|.|           |.|.:::.. :|.
Yeast   200 LRHNVDGFRIDVGSMYSKVEGLPDAPITDPTV-----PYQKGTEFFINGPRIHEYHKEMHNYMLS 259

  Fly   275 MIHHWRQLL----------DDF----SAKHPERPTRIMMTEAYAGLTQLADYYEDSNG----VRG 321
            .:...::::          |||    |||..|             |..:.::...|.|    .:.
Yeast   260 QVPEGKEIMTVGEVGIGNEDDFRVYTSAKEGE-------------LNMMFNFKHTSVGENPKCKY 311

  Fly   322 SHLPFNFHFITDVK-GDSDARDYVYNVEKW-LIYMPRGHAANWVMGNHDNPRVASRFG------- 377
            ..:||.   :.|.| ..:::..::.|.:.| .||:.          |||.||..||||
Yeast   312 ELIPFT---LKDFKLALAESFLFIENTDCWSTIYLE----------NHDQPRSVSRFGSDSPKWR 363

  Fly   378 PASVDAMNMLLLTLPGVAVTYNGEELGMVDYRELSWEE--------TVDPPARNVGE------KL 428
            ..|...:..|:::|.|....|.|:||||.:::....|:        |.....|:.||      |.
Yeast   364 EISSKMLATLIISLTGTVFIYQGQELGMPNFKNRKIEQIKCVEGTGTYAAIKRDYGEDSEKMKKF 428

  Fly   429 YQE---VSRDPVRTPFQWN-NETNAGFSTAAKTWLPVHPNYLE-LNLEAQKVANRSHYQVYKDLL 488
            ::.   :|||..||||.|: :|.:||||..||.|:.::.::.: :|.||:.....|.:..:|..|
Yeast   429 FEALALISRDHGRTPFPWSADEPSAGFSKDAKPWIDMNESFRDGINAEAELKDKNSVFFFWKKAL 493

  Fly   489 ELRK--SAIMRVG-RFNIEPLTR-WVFAF------KRSYP--NFESIITVINVSDKE 533
            ::||  ..|:..| .|....|.. .:|.|      |:.:.  ||.|..|..:|.|.|
Yeast   494 QVRKEHKDILVYGHNFQFIDLDNDKLFMFTKDTDNKKMFAVFNFSSDNTDFSVPDNE 550

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Mal-B2	NP_001188791.2	AmyAc_maltase	33..502	CDD:200467	173/535 (32%)
Mal-B2	NP_001188791.2	Malt_amylase_C	510..>541	CDD:351862	10/32 (31%)
IMA5	NP_012319.1	AmyAc_SI_OligoGlu_DGase	11..498	CDD:200472	168/521 (32%)
IMA5	NP_012319.1	Malt_amylase_C	509..>548	CDD:413446	10/38 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157342193
Domainoid	1	1.000	192	1.000	Domainoid score	I624
eggNOG	1	0.900	-	-		E1_COG0366
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	257	1.000	Inparanoid score	I641
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000282
OrthoInspector	1	1.000	-	-		mtm9159
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10357
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R680
SonicParanoid	1	1.000	-	-		X202
TreeFam	0	0.000	Not matched by this tool.
	11	10.920