Protein Alignment Mal-B2 and IMA1

DIOPT Version :9

• Sequence 1: NP_001188791.2
• Gene: Mal-B2 / 34598
• FlyBaseID: FBgn0032382
• Length: 583
• Species: Drosophila melanogaster
• Sequence 2: NP_011803.3
• Gene: IMA1 / 853204
• SGDID: S000003519
• Length: 589
• Species: Saccharomyces cerevisiae
• Alignment Length: 603
• Identity: 183/603 - (30%)
• Similarity: 296/603 - (49%)
• Gaps: 88/603 - (14%)

  Fly    34 WWPHTVFYQIYPRSFKDSNGDGIGDLKGITSKLRYLADTGITATWLSPIFQSPMIDFGYDISDYK 98
            ||....||||||.||||||.||.||:|||.|||.|:.:.|..|.|:||.:.||..|.||||::|:
Yeast    14 WWKEATFYQIYPASFKDSNDDGWGDMKGIASKLEYIKELGADAIWISPFYDSPQDDMGYDIANYE 78

  Fly    99 AIQPEYGTMQDFEELIDTAFELGIKVVLDFVPNHSSDQHEWFKKS-AAREPGYEDFYVW-----H 157
            .:.|.|||.:|...||:...:||:|.:.|.|.||.|.:|||||:| :::.....|::.|     :
Yeast    79 KVWPTYGTNEDCFALIEKTHKLGMKFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGY 143

  Fly   158 DGIVQENGTRVPPNNWPSVFYGSAWEWHEGREQYYLHQFTKEQPDLNYRNPKVVQAM-DDVLLFW 221
            |.    .|..:|||||.|.|.||||.:.|..:::||..|...|||||:.|....:|: :..:.:|
Yeast   144 DA----EGKPIPPNNWKSYFGGSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYW 204

  Fly   222 LNKGVAGFRIDAVNHLFEDESLKDEPLSGKTTDSLSYD-YTK-----HIYSRDLPE-VLEMIHHW 279
            |:.||.|||||..:...:...|.|.|:..|.:...|.| ||.     |.:.:::.: :...:...
Yeast   205 LDHGVDGFRIDVGSLYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHEFHQEMNQFIRNRVKDG 269

  Fly   280 RQLLDDFSAKHPERPTRIMMTEAYAGLTQLADYYEDSNGVRGSHLPFNFHFITDVKGD-----SD 339
            |:::.....:|....|:.:.|.  |...:|::.:..|:...|:...|.::.:.....|     ::
Yeast   270 REIMTVGEMQHASDETKRLYTS--ASRHELSELFNFSHTDVGTSPLFRYNLVPFELKDWKIALAE 332

  Fly   340 ARDYVYNVEKW-LIYMPRGHAANWVMGNHDNPRVASRFGP-------ASVDAMNMLLLTLPGVAV 396
            ...|:...:.| .||:.          |||.||..:|||.       .|...:::||..|.|...
Yeast   333 LFRYINGTDCWSTIYLE----------NHDQPRSITRFGDDSPKNRVISGKLLSVLLSALTGTLY 387

  Fly   397 TYNGEELGMVDYRELSWEETVDPPARNVGEKLYQE-----------------VSRDPVRTPFQWN 444
            .|.|:|||.::::....|:..|...||....:.:|                 :|||..|||.||:
Yeast   388 VYQGQELGQINFKNWPVEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQWS 452

  Fly   445 -NETNAGFS-TAAKTWLPVHPNYLE-LNLEAQKVANRSHYQVYKDLLELRKS-AIMRVGRFNIEP 505
             .|.||||| .:||.|..::.::.| :|:|.:.....|....:|:.|:.||: ..:.|..::.|.
Yeast   453 REEPNAGFSGPSAKPWFYLNDSFREGINVEDEIKDPNSVLNFWKEALKFRKAHKDITVYGYDFEF 517

  Fly   506 L---TRWVFAFKRSYPNFESIITVINVSDKEQLVDLSEFLSQPKKLVVEVSGVDSKYQ------P 561
            :   .:.:|:|.:.|.| :::...:|.|.     |.::|         ::...||.::      |
Yeast   518 IDLDNKKLFSFTKKYNN-KTLFAALNFSS-----DATDF---------KIPNDDSSFKLEFGNYP 567

  Fly   562 GQSLAASALLLAAREGLV 579
            .:.:.||:..|...||.:
Yeast   568 KKEVDASSRTLKPWEGRI 585

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Mal-B2	NP_001188791.2	AmyAc_maltase	33..502	CDD:200467	166/515 (32%)
		Malt_amylase_C	510..>541	CDD:351862	7/30 (23%)
IMA1	NP_011803.3	AmyAc_SI_OligoGlu_DGase	16..503	CDD:200472	161/502 (32%)
		Malt_amylase_C	514..585	CDD:413446	17/85 (20%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157342190
Domainoid	1	1.000	192	1.000	Domainoid score	I624
eggNOG	1	0.900	-	-		E1_COG0366
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	257	1.000	Inparanoid score	I641
Isobase	1	0.950	-	0.709012	Normalized mean entropy	S2995
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000282
OrthoInspector	1	1.000	-	-		mtm9159
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10357
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R680
SonicParanoid	1	1.000	-	-		X202
TreeFam	0	0.000	Not matched by this tool.
	12	11.870