DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Dlg5 and MAGI3

DIOPT Version :9

Sequence 1:	NP_609505.1	Gene:	Dlg5 / 34573	FlyBaseID:	FBgn0032363	Length:	1916	Species:	Drosophila melanogaster
Sequence 2:	NP_001136254.1	Gene:	MAGI3 / 260425	HGNCID:	29647	Length:	1481	Species:	Homo sapiens

Alignment Length:	1491	Identity:	283/1491 - (18%)
Similarity:	465/1491 - (31%)	Gaps:	463/1491 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly   467 CGVTSSSPACGKLKINDVICKVNNLDCQSLSKRMVLDEIRACAPRSLLLVSRTRHSK------RH 525
            |.|:..:|:.|     ||:.:||......|:.|..|..||..  |..:.:...:..|      ||
Human    58 CVVSGKAPSPG-----DVLLEVNGTPVSGLTNRDTLAVIRHF--REPIRLKTVKPGKVINKDLRH 115

  Fly   526 AYSVQLKTRDRDCPHGLQ--LDMGVFIAKIEQNSLAFYEPELDVGDRVLSINNKSMDSVQSIEEV 588
            ..|:|.:....|  |.||  :...:::..|...:.|..:.|:...|    .|..|::..:::||.
Human   116 YLSLQFQKGSID--HKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVD----YNFISVEQFKALEES 174

  Fly   589 MQLMNDPRSDGLNLFALKYVQDQLPPGMTTSSAQTDSIDSMQHVSSAGGGGGSGPSSATKHPSRF 653
            ..|:.....|| |.:...      .|....|..|.|.:|.:.
Human   175 GALLESGTYDG-NFYGTP------KPPAEPSPFQPDPVDQVL----------------------- 209

  Fly   654 AEFFFRKLKFSKPGTPEDNFEQEHD-----------DAIAALDSVLSENSSEKSKENLFNRKKRT 707
                               |:.|.|           ..:..:||.|.|...::.||.:.......
Human   210 -------------------FDNEFDAESQRKRTTSVSKMERMDSSLPEEEEDEDKEAINGSGNAE 255

  Fly   708 KKEKEASKSMGTWPRTNISHENPTGTMRGNEKKRALMSLFTAGPINVDKDDELMGEVGVPEKQPA 772
            .:|:.:..|  .|.:|..|:.....:|   :.:..:|...|..|:..:      .|:...:....
Human   256 NRERHSESS--DWMKTVPSYNQTNSSM---DFRNYMMRDETLEPLPKN------WEMAYTDTGMI 309

  Fly   773 ALIQDQLPPP--LPPQMSKPLAHIRGSNGGAIKTHPNRNSNPVSSGVSALFPPGPPNGMPNTSYP 835
            ..|.......  |.|::.|..........|.:                       |.|.....
Human   310 YFIDHNTKTTTWLDPRLCKKAKAPEDCEDGEL-----------------------PYGWEKIE-- 349

  Fly   836 THPRHSLYGVTAEEINQK-----PIQRAPLMGANARVKMPSQERYGTRPHSNHRLSLNITPSGDF 895
             .|::..|.|  :.:|||     |::.|      .|.|...|...|:......:......||
Human   350 -DPQYGTYYV--DHLNQKTQFENPVEEA------KRKKQLGQVEIGSSKPDMEKSHFTRDPS--- 402

  Fly   896 YQPKTSGQQAQQQVMNAS--STSAGFGVGSGSMVGG--------------TGPAAGEFPV----- 939
                    |.:..::.||  .::.|||.   :::||              .||||.:..:
Human   403 --------QLKGVLVRASLKKSTMGFGF---TIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDV 456

  Fly   940 ------------RKQQVYDVFHPPPLPKNSSGSNMFMPLHPTRGSHPPPVGQPP--DVVSLKSQN 990
                        ....|..:|...|:       |.::.|...||...|...:.|  |:|
Human   457 IVDINGNCVLGHTHADVVQMFQLVPV-------NQYVNLTLCRGYPLPDDSEDPVVDIV------ 508

  Fly   991 SIESILSAKSPAISEYGMYAKRHVPQAVRHVPKYPSDSESIGSGVHGGYGGFLQSNHMPGNRHTQ 1055
            :...:::.:|....|..|..:...|.|:.......|.....|.|::|......|...|..:..:|
Human   509 AATPVINGQSLTKGETCMNPQDFKPGAMVLEQNGKSGHTLTGDGLNGPSDASEQRVSMASSGSSQ 573

  Fly  1056 ----LFPTF-GPGGRGNRRSSPLTLPSPPPQQQLQQLPAATAPHDSVGIPTDLDYHPHHHTQTNA 1115
                ..|.. ||.|.|      ..:...|..|:::.:               ||.......|...
Human   574 PELVTIPLIKGPKGFG------FAIADSPTGQKVKMI---------------LDSQWCQGLQKGD 617

  Fly  1116 MPHPHPHPHAPYLDYGHGLGIGPYMGVGGGVSGVGGAIYEGGTFPRKKDNQRLRIPSNPSVASKS 1180
            :.....|.:...|.:...:.:.....||..|..:   |..||             |.:|:..:|.
Human   618 IIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLL---ILRGG-------------PPSPTKTAKM 666

  Fly  1181 SSMVKNSSGSID--HHYVTSTGP-----VSGGSMSASSS-----------DRAP----MSLMSSS 1223
            .:..|.::||::  :..:....|     :..||.....|           |:.|    :.:....
Human   667 KTDKKENAGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRK 731

  Fly  1224 IHNSYGANIAGGNGTGSSIGVGG----GGGGGSGR-----------GSPMP-QVHVEVL------ 1266
            ..:.:|..:.||:|...||.:|.    |.....||           |.|:. :.|.:||
Human   732 QESGFGFRVLGGDGPDQSIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTA 796

  Fly  1267 SHGG-----------------------------GGSGKRN-SNVPADFLCPGDLRRVTID-KRDK 1300
            :..|                             .||.:.| :.|||. ..|.:...|.:. |.::
Human   797 ARNGHVLLTVRRKIFYGEKQPEDDSSQAFISTQNGSPRLNRAEVPAR-PAPQEPYDVVLQRKENE 860

  Fly  1301 SLGITIQCNNN--GGGIF---VSTVADKSTAMRAG-LQVGDQLLEVCGINMRAATQEIAANVLRQ 1359
            ..|..|..:.|  ..|:.   :..|.:.|.|.|.| |:|||.:..|.|.::...:.:....:::.
Human   861 GFGFVILTSKNKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIVELSHDNIVQLIKD 925

  Fly  1360 CGDSFTMLVQYNPEKFPSIEYEGAHNLEPESPINHSGSPTPRNSPR-PPARNSLFPLPMQPQAPS 1423
            .|.:.|:.|....|                    |.|.|:..||.| .||   |...||.....:
Human   926 AGVTVTLTVIAEEE--------------------HHGPPSGTNSARQSPA---LQHRPMGQSQAN 967

  Fly  1424 TRPGSRAPLSHQSIKDQSFTDSLENQSDISSSQDMPSSAATTTTTTASATSTVYDEEPKPSLPPP 1488
            ..||.|:.|..:..||.| |....:.||       ....|...|...|...:.:::.
Human   968 HIPGDRSALEGEIGKDVS-TSYRHSWSD-------HKHLAQPDTAVISVVGSRHNQN-------- 1016

  Fly  1489 PASVPAETLRYVTLHMDKSKNLGIKLFGGNK--VGIYVHDVAVGSPSDHAG-IRKGDQILEYNGV 1550
            ....|.|..|       ..:..|..|.||.:  :|:::..:|...|:...| |..||||:|.||.
Human  1017 LGCYPVELER-------GPRGFGFSLRGGKEYNMGLFILRLAEDGPAIKDGRIHVGDQIVEINGE 1074

  Fly  1551 DLSGVTAEQAANEISKLTDTVTMLVQ--------------NKLHTLKQIKDE----PGDSFY--- 1594
            ...|:|..:|...|....:.|.:|::              |...:...|.||    |..|.:
Human  1075 PTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDINNPSSSNVIYDEQSPLPPSSHFASI 1139

  Fly  1595 ------------IRVGF------------DRTGELNEDDLRFVKDEVLYVDNT------------ 1623
                        :||..            ::...|||:... :|.:.|...|.
Human  1140 FEESHVPVIEESLRVQICEKAEELKDIVPEKKSTLNENQPE-IKHQSLLQKNVSKRDPPSSHGHS 1203

  Fly  1624 -------VFNGTFGLWRAW--KLDAMGHRKE-CGIIPSQMKVEEELR---------SGEVVDCDT 1669
                   |.||.....|:.  |..|..|.:| ...|||.:|...:.|         .||...|..
Human  1204 NKKNLLKVENGVTRRGRSVSPKKPASQHSEEHLDKIPSPLKNNPKRRPRDQSLSPSKGENKSCQV 1268

  Fly  1670 GT-ARRGSTSARRSFFRR---KKNQR---SSSRDSTE----------IASFSNTQLSFFPD 1713
            .| |..|....|:|..|.   ||.|:   |.:..:.|          ||.::.:.....||
Human  1269 STRAGSGQDQCRKSRGRSASPKKQQKIEGSKAPSNAEAKLLEGKSRRIAGYTGSNAEQIPD 1329

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Dlg5	NP_609505.1	AAA_23	<165..333	CDD:290211
		CENP-F_leu_zip	229..360	CDD:287450
		DUF1090	<326..408	CDD:284007
		PDZ_signaling	439..516	CDD:238492	14/48 (29%)
		PDZ_signaling	533..592	CDD:238492	12/60 (20%)
		PDZ_signaling	1290..1369	CDD:238492	19/85 (22%)
		PDZ_signaling	1497..1576	CDD:238492	23/81 (28%)
		SH3_DLG5	1594..1657	CDD:212794	20/111 (18%)
		Guanylate_kin	1737..1904	CDD:279019
		NK	<1773..1905	CDD:302627
MAGI3	NP_001136254.1	Interaction with ADRB1 and TGFA. /evidence=ECO:0000250	18..106		14/54 (26%)
		PDZ_signaling	24..101	CDD:238492	14/49 (29%)
		NK	123..>197	CDD:327404	17/86 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	182..273		21/141 (15%)
		WW	294..326	CDD:197736	5/37 (14%)
		WW	342..372	CDD:238122	9/34 (26%)
		Interaction with PTEN. /evidence=ECO:0000269\|PubMed:10748157	410..492		16/91 (18%)
		PDZ	413..493	CDD:214570	14/89 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	542..570		6/27 (22%)
		PDZ	575..656	CDD:214570	16/104 (15%)
		Interaction with ADGRB1. /evidence=ECO:0000269\|PubMed:10748157	726..808		16/81 (20%)
		PDZ_signaling	726..807	CDD:238492	16/80 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	821..843		5/21 (24%)
		PDZ_signaling	849..935	CDD:238492	19/85 (22%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000269\|PubMed:10748157, ECO:0000269\|PubMed:16904289	851..938		20/86 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..976		15/59 (25%)
		PDZ_signaling	1022..1100	CDD:238492	24/84 (29%)
		Red1	<1132..1461	CDD:311769	40/199 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1170..1481		37/161 (23%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0194
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810