Protein Alignment aub and Piwil1

DIOPT Version :10

• Sequence 1: NP_476734.1
• Gene: aub / 34524
• FlyBaseID: FBgn0000146
• Length: 866
• Species: Drosophila melanogaster
• Sequence 2: NP_067286.1
• Gene: Piwil1 / 57749
• MGIID: 1928897
• Length: 862
• Species: Mus musculus
• Alignment Length: 884
• Identity: 325/884 - (36%)
• Similarity: 498/884 - (56%)
• Gaps: 55/884 - (6%)

  Fly    10 ARGRGRGRKPNNVE-------ANRGFAPSLGQKSDPSHSE----GNQAS---GGNGGGGDAQVGP 60
            ||.|||.|....|:       ...|:.|...|:| |:..:    |.|..   |......:.|:..
Mouse     7 ARARGRARGQETVQHVGAAASQQPGYIPPRPQQS-PTEGDLVGRGRQRGMVVGATSKSQELQISA 70

  Fly    61 SIEKSSLSAVQMHKSEGDPRGSVRGRRLITDLVYSRPPGM----TSKKGVVGTHITVQANYFKVL 121
            ..::.||:          .||.  .||...||..:....:    .||.|..|..:.:..|:|::.
Mouse    71 GFQELSLA----------ERGG--RRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLT 123

  Fly   122 KRPNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILGG-YIFDGTNMFCINQFKAVQDSPYVLELV 185
            .||.|.:|||.:|:.|.:||.|||.:.|::|:.::|. :.||||.:|..   |.:|..  |.|:.
Mouse   124 SRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLP---KRLQHK--VTEVF 183

  Fly   186 TKSRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYYDPQAKINLENFRMQ 250
            :::|.||::.|.|.....:..|.....|..|:|.||.::.::|:.:.|.||:|...|::.|.|:.
Mouse   184 SQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLV 248

  Fly   251 LWPGYQTSIRQHENDILLCSEICHKVMRTETLYNILSDAIRDSDD--YQSTFKRAVMGMVILTDY 313
            :|||:.|||.|:||:|:||:::.|||:|:||:.:.:.:..:.:::  :|....:.::|:::||.|
Mouse   249 IWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKY 313

  Fly   314 NNKTYRIDDVDFQSTPLCKFKTNDG-EISYVDYYKKRYNIIIRDLKQPLVMSRPTDKNIRGGN-D 376
            ||||||:||:|:...|...||..|| |:|:::||:|:||..|.|||||:::|:|..:...||. .
Mouse   314 NNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLP 378

  Fly   377 QAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSWN 441
            ...|:||||...||:||.||.||..::.::.||||.|::|...:......:.........|:.|.
Mouse   379 GPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWG 443

  Fly   442 IELDSALVEIPARVLPPEKILFGNQKIFVCDARADWTNEFRTCSMFKNVHINRWYVITPSRNLRE 506
            :..||.|:....|:|..|||..|.:........|||:.|.|...:.....::.|.:|...||...
Mouse   444 LSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEA 508

  Fly   507 TQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSCI 571
            ....:|...:...:|.:.:...|..|: |||...|.:|:.....:|.|||:.::.|..::||..|
Mouse   509 ANSLIQNLFKVTPAMGIQMKKAIMIEV-DDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAI 572

  Fly   572 KKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGFD 636
            ||..|.|.|.|||.|..:.:..:|.    :|:||||:.:|||.|:.|..|:|.:||...|.||.|
Mouse   573 KKYLCTDCPTPSQCVVARTLGKQQT----VMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGID 633

  Fly   637 VCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPER 701
            ..|......:|...|||::::..: |:||......:||||.:.:.|.:..|||::...:..:|.|
Mouse   634 CYHDTTAGRRSIAGFVASINEGMT-RWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSR 697

  Fly   702 ILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYF--TGHR-- 762
            ::.:|||||||||..:||.||....|.|    ||.|:....|:|.|:|.||:|:|:|  :|.|
Mouse   698 VIVYRDGVGDGQLKTLVNYEVPQFLDCL----KSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQ 758

  Fly   763 NPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADKLQMLSYKMTHMYYNY 827
            ||:||||:|..:|.||.||||:||||||.|:||||.||||.|:.||..|.:|.|:||:.|:|||:
Mouse   759 NPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNW 823

  Fly   828 SGTIRVPAVCHYAHKLAFLVAESINRAPSAGLQNQLYFL 866
            .|.|||||.|.|||||||||.:||:|.|:..|.|:||:|
Mouse   824 PGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL 862

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
aub	NP_476734.1	PAZ_piwi_like	272..392	CDD:239211	49/123 (40%)
		Piwi_piwi-like_Euk	400..849	CDD:240016	175/452 (39%)
Piwil1	NP_067286.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48		12/41 (29%)
		GAGE	16..107	CDD:461753	20/103 (19%)
		D-box. /evidence=ECO:0000269|PubMed:23328397	218..225		2/6 (33%)
		ArgoL1	229..276	CDD:462567	22/46 (48%)
		PAZ	278..416	CDD:198017	55/137 (40%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269|PubMed:21237665	317..319		1/1 (100%)
		Piwi_piwi-like_Euk	402..845	CDD:240016	175/452 (39%)
		MID region. /evidence=ECO:0000305|PubMed:24757166	480..616		42/140 (30%)