DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment aub and piwil1

DIOPT Version :9

Sequence 1:	NP_476734.1	Gene:	aub / 34524	FlyBaseID:	FBgn0000146	Length:	866	Species:	Drosophila melanogaster
Sequence 2:	NP_899181.1	Gene:	piwil1 / 368200	ZFINID:	ZDB-GENE-030813-2	Length:	858	Species:	Danio rerio

Alignment Length:	885	Identity:	315/885 - (35%)
Similarity:	495/885 - (55%)	Gaps:	63/885 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    10 ARGRGRGRKPNNVEANRGFAPSLGQK------SDPSHSE----GNQ--ASGGNGGGGDAQVGPSI 62
            :||||||::|    |..|..|.:.|:      |.||..:    |.|  |.|........|:....
Zfish     9 SRGRGRGQEP----AAPGAQPPVSQEAAKPVVSTPSEGQLVGRGRQKPAPGAMSEEAMLQISAGF 69

  Fly    63 EKSSLSAVQMHKSEGDPR----GSVRGRRLITDLVYSRPPGMTSKKGVVGTHITVQANYFKVLKR 123
            ::     |::.:..|..|    ..|..|:|:..:       ..||.||.||.|.::||:.::|.|
Zfish    70 QQ-----VKIGERGGRRRDFHDSGVHTRQLMEHV-------KESKTGVSGTAIELRANFMRLLSR 122

  Fly   124 PNWTIYQYRVDFTPDVEATRLRRSFLYEHKGILG-GYIFDGTNMFCINQFKAVQDSPYVLELVTK 187
            |.|.:|||.||:.|.:|:.|||.:.|::|:..|| .:.|||..:|..|:.:..:     ..|.::
Zfish   123 PMWALYQYHVDYKPPMESRRLRSALLFQHEETLGKAHTFDGAILFLPNKLRNAE-----TVLCSE 182

  Fly   188 SRAGENIEIKIKAVGSVQSTDAEQFQVLNLILRRAMEGLDLKLVSRYYYDPQAKINLENFRMQLW 252
            :|.||.:||.:.....:..:.....|..|::.||.:..|:::.:.|:||:|....|:...|:.:|
Zfish   183 TRNGEKVEITVTLTNELPPSSPVCLQFYNILFRRILRILNMQQIGRHYYNPDDPFNIPQHRLTIW 247

  Fly   253 PGYQTSIRQHENDILLCSEICHKVMRTETLYNILSDAIRDSDD--YQSTFKRAVMGMVILTDYNN 315
            ||:.|:|.|:|:.|:|||::.|||:|:||:.:.:....:...|  :.....:.::|::|||.|||
Zfish   248 PGFMTTILQYESSIMLCSDVSHKVLRSETVLDFMYSLRQQCGDQRFPEACTKELVGLIILTKYNN 312

  Fly   316 KTYRIDDVDFQSTPLCKFKTNDGEISYVDYYKKRYNIIIRDLKQPLVMSR-----PTDKNIRGGN 375
            |||||||:.:..||...||..|.|||:.:|:|.:|.:.|.|..|.|::|.     |:.:...|
Zfish   313 KTYRIDDIAWDHTPNNTFKKGDTEISFKNYFKSQYGLDITDGNQVLLVSHVKRLGPSGRPPPG-- 375

  Fly   376 DQAIMIIPELARATGMTDAMRADFRTLRAMSEHTRLNPDRRIERLRMFNKRLKSCKQSVETLKSW 440
              ..|::||....||:||.|||||..::.::.||||:|::|..|:......:.........|.:|
Zfish   376 --PAMLVPEFCYLTGLTDKMRADFNIMKDLASHTRLSPEQREGRINRLISNINRNGDVQNELTTW 438

  Fly   441 NIELDSALVEIPARVLPPEKILFGNQKIFVCDARADWTNEFRTCSMFKNVHINRWYVITPSRNLR 505
            .:..::.|:.:..||||.|:|:.|.:........|||:.|.|...:...:.::.|.:....||..
Zfish   439 GLSFENKLLSLNGRVLPSERIIQGGRAFEYNPWTADWSKEMRGLPLISCMSLDNWLMFYTRRNAD 503

  Fly   506 ETQEFVQMCIRTASSMKMNICNPIYEEIPDDRNGTYSQAIDNAAANDPQIVMVVMRSPNEEKYSC 570
            ..|..:|...:.:..|.:.:...:..|. :||..:..:|:....|.:.|:|:|::.:..::||.|
Zfish   504 VAQSLLQTLNKVSGPMGIRMQRAVMIEY-EDRQESLLRALQQNVARETQMVVVILPTNRKDKYDC 567

  Fly   571 IKKRTCVDRPVPSQVVTLKVIAPRQQKPTGLMSIATKVVIQMNAKLMGAPWQVVIPLHGLMTVGF 635
            :||..|||.|.|||.|..:.|:    ||..||::|||:.:|||.|:.|..|.|.|||..||.||.
Zfish   568 VKKYLCVDCPTPSQCVVSRTIS----KPQALMTVATKIALQMNCKMGGELWSVEIPLRQLMIVGI 628

  Fly   636 DVCHSPKNKNKSYGAFVATMDQKESFRYFSTVNEHIKGQELSEQMSVNMACALRSYQEQHRSLPE 700
            |..|......:|.||.||:::|..| |:||......:|||:.:.:..::..||::|.:.:.|||.
Zfish   629 DCYHDTAAGKRSIGAMVASLNQGMS-RWFSKCVLQNRGQEIIDALKGSLQGALKAYLKYNNSLPS 692

  Fly   701 RILFFRDGVGDGQLYQVVNSEVNTLKDRLDEIYKSAGKQEGCRMTFIIVSKRINSRYFT---GH- 761
            ||:.:|||||||.|..||:.||..:...:    |:.|:....:::.::|.|||:||:|.   |.
Zfish   693 RIIVYRDGVGDGMLQSVVDYEVPQIMQSI----KTMGQDYEPKLSVVVVKKRISSRFFARIDGKI 753

  Fly   762 RNPVPGTVVDDVITLPERYDFFLVSQAVRIGTVSPTSYNVISDNMGLNADKLQMLSYKMTHMYYN 826
            .||.||||:|..:|.||.||||:||||||.|.|:||.|||:.||.||..|.:|.|:||:.|||||
Zfish   754 ANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYNVVFDNSGLKPDHMQRLTYKLCHMYYN 818

  Fly   827 YSGTIRVPAVCHYAHKLAFLVAESINRAPSAGLQNQLYFL 866
            :.|.:||||.|.|||||||||.:||::.|:..|.:.||:|
Zfish   819 WQGIVRVPAPCQYAHKLAFLVGQSIHKEPNMNLDDFLYYL 858

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
aub	NP_476734.1	ArgoN	105..219	CDD:293095	38/114 (33%)
		PAZ_piwi_like	272..392	CDD:239211	44/126 (35%)
		Piwi_piwi-like_Euk	400..849	CDD:240016	172/452 (38%)
piwil1	NP_899181.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..56		17/50 (34%)
		GAGE	17..104	CDD:283487	21/102 (21%)
		ArgoN	104..203	CDD:293095	36/103 (35%)
		PAZ	275..412	CDD:198017	51/140 (36%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	314..316		1/1 (100%)
		Piwi_piwi-like_Euk	398..841	CDD:240016	172/452 (38%)
		MID region. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	476..612		41/140 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580666
Domainoid	1	1.000	146	1.000	Domainoid score	I4499
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H37963
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	LDO	PTHR22891
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3665
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.750