DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment piwi and PIWIL1

DIOPT Version :9

Sequence 1:	NP_001285825.1	Gene:	piwi / 34521	FlyBaseID:	FBgn0004872	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_004755.2	Gene:	PIWIL1 / 9271	HGNCID:	9007	Length:	861	Species:	Homo sapiens

Alignment Length:	862	Identity:	312/862 - (36%)
Similarity:	484/862 - (56%)	Gaps:	72/862 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERK 70
            ||||:|       .|:.|:.....:::..|                    .:|....|.|...|
Human    48 GRGRQR-------GTAGGTAKSQGLQISAG--------------------FQELSLAERGGRRR- 84

  Fly    71 PWGDQYDY-LNTRP-----AELVSKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPR 129
               |.:|. :|||.     .|  ||.|:.|:.|.|.||.|||.::|:|.:..||:::.|.:|..|
Human    85 ---DFHDLGVNTRQNLDHVKE--SKTGSSGIIVRLSTNHFRLTSRPQWALYQYHIDYNPLMEARR 144

  Fly   130 VRMGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFL 194
            :|..:|..|.:|:|..:.|||..||..::.:|::|.:..|::...:.:|:|.....:....|..|
Human   145 LRSALLFQHEDLIGKCHAFDGTILFLPKRLQQKVTEVFSKTRNGEDVRITITLTNELPPTSPTCL 209

  Fly   195 QVLNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVM 259
            |..|:|.||.:|.:||:.:|||.::|...|:|...::.:|||:.|||.|:|..|:|.|:::|||:
Human   210 QFYNIIFRRLLKIMNLQQIGRNYYNPNDPIDIPSHRLVIWPGFTTSILQYENSIMLCTDVSHKVL 274

  Fly   260 RTETIYDIMRRCSHNPARH--QDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTF-SCKGRD 321
            |:||:.|.|....|....|  |::|...::.|:|||.|||:|||::|:|:.|.||||| ...|.:
Human   275 RSETVLDFMFNFYHQTEEHKFQEQVSKELIGLVVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSE 339

  Fly   322 ISFVEYYLTKYNIRIRDHNQPLLISK-NRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMR 385
            :||:|||..:||..|.|..||:|:|: .|.:..........:||||||.:|||..:||::|.:|:
Human   340 VSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMK 404

  Fly   386 AMSSYTRMNPKQR---TDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHN 447
            .::.:||:.|:||   ..||..:.|:..|..   :.||||.:..|.|:....|||:..:.|  |.
Human   405 DLAVHTRLTPEQRQREVGRLIDYIHKNDNVQ---RELRDWGLSFDSNLLSFSGRILQTEKI--HQ 464

  Fly   448 GKVPAGEN---ADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIR 509
            |......|   |||.:..|...:::...  ||.|.:|..:||.....:|:.:|::....||:::|
Human   465 GGKTFDYNPQFADWSKETRGAPLISVKP--LDNWLLIYTRRNYEAANSLIQNLFKVTPAMGMQMR 527

  Fly   510 SPQEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTT 574
            . ...|..||||..|:|.:...|.:|.::::||:.::..::|.:|||....|...|:|.|..:|.
Human   528 K-AIMIEVDDRTEAYLRVLQQKVTADTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTL 591

  Fly   575 -KNRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQN 638
             |.:::|:||||||:|:|||:|...|.:::||..:|.:|.|........:|:....:||:: :..
Human   592 GKQQTVMAIATKIALQMNCKMGGELWRVDIPLKLVMIVGIDCYHDMTAGRRSIAGFVASIN-EGM 655

  Fly   639 STYFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDI 703
            :.:||..........|.:.|...:..|||.:...:..:||||:.|||||..|.||.|..:||...
Human   656 TRWFSRCIFQDRGQELVDGLKVCLQAALRAWNSCNEYMPSRIIVYRDGVGDGQLKTLVNYEVPQF 720

  Fly   704 IEKLKT---EYARVQLSPPQLAYIVVTRSMNTRFFLNG----QNPPPGTIVDDVITLPERYDFYL 761
            ::.||:   .|      .|:|..|||.:.:|||||...    |||.|||::|..:|.||.|||::
Human   721 LDCLKSIGRGY------NPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFI 779

  Fly   762 VSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGT 826
            |||.||.|:||||.|||:|.:.||.|:.:|:||||:||:||||.|..||||.||||.|||.|||.
Human   780 VSQAVRSGSVSPTHYNVIYDNSGLKPDHIQRLTYKLCHIYYNWPGVIRVPAPCQYAHKLAFLVGQ 844

  Fly   827 NLHSIPQNALEKKFYYL 843
            ::|..|..:|..:.|||
Human   845 SIHREPNLSLSNRLYYL 861

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_001285825.1	ArgoN	91..201	CDD:293095	35/109 (32%)
		PAZ	262..396	CDD:198017	56/137 (41%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	170/457 (37%)
PIWIL1	NP_004755.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..64		7/22 (32%)
		GAGE	16..106	CDD:310430	20/90 (22%)
		D-box. /evidence=ECO:0000269\|PubMed:28552346	217..224		3/6 (50%)
		ArgoL1	227..273	CDD:312283	18/45 (40%)
		PAZ	277..415	CDD:198017	56/137 (41%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269\|PubMed:21193640	316..318		1/1 (100%)
		Piwi_piwi-like_Euk	401..844	CDD:240016	170/457 (37%)
		MID region. /evidence=ECO:0000250\|UniProtKB:Q9JMB7	479..615		45/138 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165147172
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	1	1.010	-	-		D220258at2759
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.720