DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment piwi and AGO2

DIOPT Version :9

Sequence 1:	NP_001285825.1	Gene:	piwi / 34521	FlyBaseID:	FBgn0004872	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_174413.2	Gene:	AGO2 / 840016	AraportID:	AT1G31280	Length:	1014	Species:	Arabidopsis thaliana

Alignment Length:	1021	Identity:	221/1021 - (21%)
Similarity:	388/1021 - (38%)	Gaps:	271/1021 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly     4 DQGRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDP------RADPRIEASRERRALE--EAP 60
            :|||||    ..:....:||.|.|.: :.||..|...|      |...:.:..|.:.|.:  :||
plant    40 EQGRGR----GSERGGGNRGQGRGEQ-QDFRSQSQRGPPPGHGGRGTTQFQQPRPQVAPQPSQAP 99

  Fly    61 R----REGGPTERKPWGDQ-----------------------YDYLNTRPA---------ELVSK 89
            .    ..||...|..||.:                       .:.:|.:|:         |.:.:
plant   100 ASYAGSVGGVAGRGAWGRKPQVPSDSASPSTSTTVVSEPVRVAEVMNLKPSVQVATSDRKEPMKR 164

  Fly    90 KGTDGV----PVMLQTNFFRLKTKPEWRIVHYHVEFEPSI--------ENPRVRMGVLSNHANLL 142
            ....||    .|.|..|.:::...||..|.||.||.:..|        |...||..|.:::.:..
plant   165 PDRGGVVAVRRVNLYVNHYKVNFNPESVIRHYDVEIKGEIPTKKVSRFELAMVRDKVFTDNPDEF 229

  Fly   143 --------GSGYLFDGLQLFT-TRKFEQEIT-VLSGKSKLDIEYKISIKFVGFISCAEPRFLQVL 197
                    |...:|..::|.| :.|.|...| .:.|:|     |..:||.|..:...:
plant   230 PLAMTAYDGQKNIFSAVELPTGSYKVEYPKTEEMRGRS-----YTFTIKQVNVLKLGD------- 282

  Fly   198 NLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWP-------GYETSIRQHEKD------IL 249
                      |...:.||:.|:||..::..:..|:..|       |.....|:.|.|      ::
plant   283 ----------LKEYMTGRSSFNPRDVLQGMDVVMKEHPSKCMITVGKSFFTRETEPDEDFRFGVI 337

  Fly   250 LGTEITHKVMRTE------TIYDIM--RRC--------------SHNPARHQD--------EVRV 284
            ......|.:..|.      ..|.::  |:.              .....|.:|        :|.|
plant   338 AAKGYRHTLKPTAQGLSLCLDYSVLAFRKAMSVIEYLKLYFNWSDMRQFRRRDVEEELIGLKVTV 402

  Fly   285 N------VLDLIVLTDYNNRTYRIN--DVDFGQTPKSTFSCKGRDISFVEYYLTKYNIRIRDHNQ 341
            |      .|.::.|:..|.:..:.:  |.:..:.|:.|        |.|||:..||...|...:.
plant   403 NHRKNKQKLTIVGLSMQNTKDIKFDLIDQEGNEPPRKT--------SIVEYFRIKYGRHIVHKDI 459

  Fly   342 PLL-ISKNRDKALKTNASELVVLIP-ELCRVTGLNAEMRSNFQLMRA--MSSYTRMNPKQR---T 399
            |.| :.||.    :.|      .:| |.|.:.......:.|.....|  :...:.:||:||   .
plant   460 PCLDLGKNG----RQN------FVPMEFCDLVEGQIYPKDNLDKDSALWLKKLSLVNPQQRQRNI 514

  Fly   400 DRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNI-VFHNGKVPAGE-----NADW 458
            |::    .:.:|.|...:::.::.:::|.|:|.|:||::...:: :...|:|...|     |..|
plant   515 DKM----IKARNGPSGGEIIGNFGLKVDTNMTPVEGRVLKAPSLKLAERGRVVREEPNPRQNNQW 575

  Fly   459 QRHFRDQRMLTTPSDGLDRWAVI---APQRNSHELRTLLDSLYRAASGMGLRIRSPQEFIIYDD- 519
            ..    .:...|....:..|||:   |.:|.:......:|:|......:|:::.:|   |:|..
plant   576 NL----MKKGVTRGSIVKHWAVLDFTASERFNKMPNDFVDNLIDRCWRLGMQMEAP---IVYKTS 633

  Fly   520 --RTGTYVRAMDDCVRS------------DPKLILCLVPNDNAERYSSIK-----KRGYVDRAVP 565
              .|.:...|:::.:||            .|.|:||.:...: :.|.::|     |.|.|.:...
plant   634 RMETLSNGNAIEELLRSVIDEASRKHGGARPTLVLCAMSRKD-DGYKTLKWIAETKLGLVTQCFL 697

  Fly   566 TQVVTLKTTKNRSLMSIATKIAIQLNCKLGYTPWMIEL--------PLSGLMTIGFDI-AKSTRD 621
            |...|....:.|:      .:|:::|.|:|.:  .:||        ....:|.||.|: ..:.||
plant   698 TGPATKGGDQYRA------NLALKMNAKVGGS--NVELMDTFSFFKKEDEVMFIGADVNHPAARD 754

  Fly   622 R-KRAYGALIASMDLQQNSTYFSTV-------TECSAFDVLANTLWPMIAKALRQYQHEHRKLPS 678
            : ..:..|::.:::..:.:.|.:.|       .|...|.       ....:.::.:.....|.|:
plant   755 KMSPSIVAVVGTLNWPEANRYAARVIAQPHRKEEIQGFG-------DACLELVKAHVQATGKRPN 812

  Fly   679 RIVFYRDGVSSGSLKQLFEFEVKDIIEKL---KTEYARVQLSPPQLAYIVVTRSMNTRFF--LNG 738
            :||.:|||||......:...|:.|:  ||   |..|      .|::..||..:...||||  .|.
plant   813 KIVIFRDGVSDAQFDMVLNVELLDV--KLTFEKNGY------NPKITVIVAQKRHQTRFFPATNN 869

  Fly   739 Q-----NPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMC 798
            .     |.|.||:||..:..|..|||||.|.....||..||.|..|:..:|.:.:::|||.::||
plant   870 DGSDKGNVPSGTVVDTKVIHPYEYDFYLCSHHGGIGTSKPTHYYTLWDELGFTSDQVQKLIFEMC 934

  Fly   799 HLYYNWSGTTR----VPAVCQYAKKLATLVGTNLHSIPQNALEKKF 840
               :.::..|:    ||.|  |...:....|...|.  .::.||.|
plant   935 ---FTFTRCTKPVSLVPPV--YYADMVAFRGRMYHE--ASSREKNF 973

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_001285825.1	ArgoN	91..201	CDD:293095	30/131 (23%)
		PAZ	262..396	CDD:198017	31/175 (18%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	117/508 (23%)
AGO2	NP_174413.2	PLN03202	137..957	CDD:215631	193/899 (21%)
		ArgoN	164..306	CDD:293095	36/163 (22%)
		ArgoL1	317..366	CDD:285861	7/48 (15%)
		PAZ	383..488	CDD:280352	26/122 (21%)
		Piwi_ago-like	528..963	CDD:240015	110/470 (23%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	4	3.880