DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment piwi and Piwil1

DIOPT Version :9

Sequence 1:	NP_001285825.1	Gene:	piwi / 34521	FlyBaseID:	FBgn0004872	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_067286.1	Gene:	Piwil1 / 57749	MGIID:	1928897	Length:	862	Species:	Mus musculus

Alignment Length:	857	Identity:	312/857 - (36%)
Similarity:	486/857 - (56%)	Gaps:	61/857 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly     6 GRGRRRPLNEDDSSTSRGSGDGPRVKVFRGSSSGDPRADPRIEASRERRALEEAPRREGGPTERK 70
            ||||:|.:....:|.|:                     :.:|.|..:..:|.|    .||  .|:
Mouse    48 GRGRQRGMVVGATSKSQ---------------------ELQISAGFQELSLAE----RGG--RRR 85

  Fly    71 PWGDQYDY-LNTRP-AELV--SKKGTDGVPVMLQTNFFRLKTKPEWRIVHYHVEFEPSIENPRVR 131
               |.:|. :|||. .:.|  ||.|:.|:.|.|.||.|||.::|:|.:..||:::.|.:|..|:|
Mouse    86 ---DFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLR 147

  Fly   132 MGVLSNHANLLGSGYLFDGLQLFTTRKFEQEITVLSGKSKLDIEYKISIKFVGFISCAEPRFLQV 196
            ..:|..|.:|:|..:.|||..||..::.:.::|.:..:::.....:|:|.....:....|..||.
Mouse   148 SALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQF 212

  Fly   197 LNLILRRSMKGLNLELVGRNLFDPRAKIEIREFKMELWPGYETSIRQHEKDILLGTEITHKVMRT 261
            .|:|.||.:|.:||:.:|||.::|...|:|...::.:|||:.|||.|:|.:|:|.|:::|||:|:
Mouse   213 YNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRS 277

  Fly   262 ETIYDIMRRCSHNPARH--QDEVRVNVLDLIVLTDYNNRTYRINDVDFGQTPKSTF-SCKGRDIS 323
            ||:.|.|.........|  |::|...::.|||||.|||:|||::|:|:.|.||||| ...|.::|
Mouse   278 ETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVS 342

  Fly   324 FVEYYLTKYNIRIRDHNQPLLISK-NRDKALKTNASELVVLIPELCRVTGLNAEMRSNFQLMRAM 387
            |:|||..:||..|.|..||:|:|: .|.:..........:||||||.:|||..:||::|.:|:.:
Mouse   343 FLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDL 407

  Fly   388 SSYTRMNPKQR---TDRLRAFNHRLQNTPESVKVLRDWNMELDKNVTEVQGRIIGQQNIVFHNGK 449
            :.:||:.|:||   ..||..:.|:..|..   :.||||.:..|.|:....|||:..:.|  |.|.
Mouse   408 AVHTRLTPEQRQREVGRLIDYIHKDDNVQ---RELRDWGLSFDSNLLSFSGRILQSEKI--HQGG 467

  Fly   450 VPAGEN---ADWQRHFRDQRMLTTPSDGLDRWAVIAPQRNSHELRTLLDSLYRAASGMGLRIRSP 511
            .....|   |||.:..|...:::...  ||.|.:|..:||.....:|:.:|::....||::::.
Mouse   468 KTFDYNPQFADWSKETRGAPLISVKP--LDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKK- 529

  Fly   512 QEFIIYDDRTGTYVRAMDDCVRSDPKLILCLVPNDNAERYSSIKKRGYVDRAVPTQVVTLKTT-K 575
            ...|..||||..|:||:...|.||.::::||:.::..::|.:|||....|...|:|.|..:|. |
Mouse   530 AIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGK 594

  Fly   576 NRSLMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQNST 640
            .:::|:||||||:|:|||:|...|.:::||...|.:|.|....|...:|:....:||:: :..:.
Mouse   595 QQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASIN-EGMTR 658

  Fly   641 YFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKDIIE 705
            :||..........|.:.|...:..|||.:...:..:|||::.|||||..|.||.|..:||...::
Mouse   659 WFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLD 723

  Fly   706 KLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNG----QNPPPGTIVDDVITLPERYDFYLVSQQV 766
            .||:......   |:|..|||.:.:|.|||...    |||.|||::|..:|.||.|||::|||.|
Mouse   724 CLKSVGRGYN---PRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAV 785

  Fly   767 RQGTVSPTSYNVLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSI 831
            |.|:||||.|||:|.|.||.|:.:|:||||:||:||||.|..||||.||||.|||.|||.::|..
Mouse   786 RSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHRE 850

  Fly   832 PQNALEKKFYYL 843
            |..:|..:.|||
Mouse   851 PNLSLSNRLYYL 862

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
piwi	NP_001285825.1	ArgoN	91..201	CDD:293095	33/109 (30%)
		PAZ	262..396	CDD:198017	56/137 (41%)
		Piwi_piwi-like_Euk	382..826	CDD:240016	170/454 (37%)
Piwil1	NP_067286.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48		312/857 (36%)
		GAGE	16..107	CDD:399085	22/88 (25%)
		D-box. /evidence=ECO:0000269\|PubMed:23328397	218..225		3/6 (50%)
		PAZ	278..416	CDD:198017	56/137 (41%)
		Required for binding 2'-O-methylated 3'-end of piRNAs. /evidence=ECO:0000269\|PubMed:21237665	317..319		1/1 (100%)
		Piwi_piwi-like_Euk	402..845	CDD:240016	170/454 (37%)
		MID region. /evidence=ECO:0000305\|PubMed:24757166	480..616		46/138 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837118
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG1042
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54391
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0003636
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100694
Panther	1	1.100	-	-	O	PTHR22891
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X548
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	10	9.710